Formation of N-pyroglutamyl peptides from N-Glu and N-Gln precursors in Aplysia neurons.
J Neurochem
; 72(2): 676-81, 1999 Feb.
Article
em En
| MEDLINE
| ID: mdl-9930740
ABSTRACT
Matrix-assisted laser desorption/ionization with time-of-flight mass spectrometry is used to examine the formation of N-pyroglutamate (pGlu) in single, identified neurons from Aplysia. Six pGlu peptides are identified in the R3-14 and the R15 neurons that result from in vivo processing of peptides containing either Glu or Gln at their respective N-termini. Moreover, we show that Glu-derived pGlu is not a sample collection or measurement artifact. The pGlu peptides are detected in isolated cell bodies, regenerated neurites in culture, interganglionic connective nerves, cell homogenates, and collected releasates. We also demonstrate that R3-14 cells readily convert a synthetic N-Glu peptide to its pGlu analogue, indicating the presence of novel enzymatic activity.
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Base de dados:
MEDLINE
Assunto principal:
Ácido Pirrolidonocarboxílico
/
Ácido Glutâmico
/
Glutamina
/
Neurônios
Idioma:
En
Ano de publicação:
1999
Tipo de documento:
Article