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Comprehensive RNA dataset of AGO2 associated RNAs in Jurkat cells following miR-21 over-expression.

Carissimi, Claudia; Colombo, Teresa; Azzalin, Gianluca; Cipolletta, Emanuela; Laudadio, Ilaria; Macino, Giuseppe; Fulci, Valerio.
Data Brief ; 7: 604-6, 2016 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-27054165
We set out to identify miR-21 targets in Jurkat cells using a high-throughput biochemical approach (10.1016/j.biochi.2014.09.021[1]). Using a specific monoclonal antibody raised against AGO2, RISC complexes were immunopurified in Jurkat cells over-expressing miR-21 following lentiviral trasduction as well as in Jurkat control cells lines. A parallel immunoprecipitation using isotype-matched rat IgG was performed as a control. AGO2 associated mRNAs were profiled by microarray (GEO GSE37212). AGO2 bound miRNAs were profiled by RNA-seq.