Dispersal of remnant endangered trees in a fragmented and disturbed forest by frugivorous birds.

Most endangered plant species in a fragmented forest behave as a unique source population, with a high dependence on frugivorous birds for recruitment and persistence. In this study, we combined field data of dispersal behavior of birds and GIS information of patch attributes to estimate how frugivorous birds could affect the effective dispersal pattern of Chinese yew (Taxus chinensis) in a fragmented and disturbed forest. Nine bird species were observed to visit T. chinensis trees, with Urocissa erythrorhyncha, Zoothera dauma and Picus canus being the most common dispersers. After foraging, six disperser species exhibited different perching patterns. Three specialist species, P. canus, Turdus hortulorum, and Z. dauma stayed in the source patch, while three generalist species, U. erythrorhyncha, Hypsipetes mcclellandii, and H. castanonotus, could perch in bamboo patches and varied in movement ability due to body size. As a consequence of perching, dispersers significantly contributed to the seed bank, but indirectly affected seedling recruitment. Moreover, the recruitment of T. chinensis was also affected by patch attributes in a fragmented forest (distances to source patch, patch type, size). Our results highlighted the ability of unique source population regeneration of T. chinensis in a fragmented forest, with high dependence on both frugivorous birds and patch attributes, which should be considered in future planning for forest management and conservation.

Development and characterization of polymorphic microsatellite markers (SSRs) for an endemic plant, Pseudolarix amabilis (Nelson) Rehd. (Pinaceae).

Pseudolarix (Pinaceae) is a vulnerable (sensu IUCN) monotypic genus restricted to southeastern China. To better understand levels of genetic diversity, population structure and gene flow among populations of P. amabilis, we developed five compound SSR markers and ten novel polymorphic expressed sequence tags (EST) derived microsatellites. The results showed that all 15 loci were polymorphic with the number of alleles per locus ranging from two to seven. The expected and observed heterozygosities varied from 0.169 to 0.752, and 0.000 to 1.000, respectively. The inbreeding coefficient ranged from -0.833 to 1.000. These markers will contribute to research on genetic diversity and population genetic structure of P. amabilis, which in turn will contribute to the species conservation.

[Ultra-Fine Pressed Powder Pellet Sample Preparation XRF Determination of Multi-Elements and Carbon Dioxide in Carbonate].

The main analysis error of pressed powder pellet of carbonate comes from particle-size effect and mineral effect. So in the article in order to eliminate the particle-size effect, the ultrafine pressed powder pellet sample preparation is used to the determination of multi-elements and carbon-dioxide in carbonate. To prepare the ultrafine powder the FRITSCH planetary Micro Mill machine and tungsten carbide media is utilized. To conquer the conglomeration during the process of grinding, the wet grinding is preferred. The surface morphology of the pellet is more smooth and neat, the Compton scatter effect is reduced with the decrease in particle size. The intensity of the spectral line is varied with the change of the particle size, generally the intensity of the spectral line is increased with the decrease in the particle size. But when the particle size of more than one component of the material is decreased, the intensity of the spectral line may increase for S, Si, Mg, or decrease for Ca, Al, Ti, K, which depend on the respective mass absorption coefficient . The change of the composition of the phase with milling is also researched. The incident depth of respective element is given from theoretical calculation. When the sample is grounded to the particle size of less than the penetration depth of all the analyte, the effect of the particle size on the intensity of the spectral line is much reduced. In the experiment, when grounded the sample to less than 8 µm(d95), the particle-size effect is much eliminated, with the correction method of theoretical α coefficient and the empirical coefficient, 14 major, minor and trace element in the carbonate can be determined accurately. And the precision of the method is much improved with RSD < 2%, except Na2O. Carbon is ultra-light element, the fluorescence yield is low and the interference is serious. With the manual multi-layer crystal PX4, coarse collimator, empirical correction, X-ray spectrometer can be used to determine the carbon dioxide in the carbonate quantitatively. The intensity of the carbon is increase with the times of the measurement and the time delay even the pellet is stored in the dessicator. So employing the latest pressed powder pellet is suggested.

[Microplastic Characteristics and Risk Assessment in Multigate Dam-type River].

Microplastics pose a serious ecological threat to rivers in China, and the construction of a large number of dams has complicated this problem. Ten dams of the Shaying River were chosen to investigate the abundance and composition of microplastics in surface water and sediments of the reservoir and upstream river. Ecological risk was evaluated using species sensitive distribution （SSD） and pollution load index （PLI）. The results showed that the Shaying River was exposed to a severe risk of microplastics from upstream to downstream. The construction of dams did not significantly affect the distribution of microplastics in the river. River sediments became a sink for microplastics in the surface water； however, the ecological risk posed by microplastics in the surface water was greater, and the comparison of the two assessment methods showed that the species sensitivity distribution assessment better reflected the accumulation and feeding behavior of organisms to pollutants compared to the pollution load index.

Polycyclic aromatic hydrocarbons in surface sediments of the Jialu River.

The Jialu River, an important branch of the Huaihe River in China, was seriously polluted because of rapid economic growth and urbanization. In order to evaluate the potential for serious environmental consequences as a result of anthropogenic contamination, the distribution of polycyclic aromatic hydrocarbons (PAHs) has been investigated in surface sediment samples collected in connection with field surveys of 19 sites along the Jialu River. The total concentration of the 16 USEPA priority PAHs ranged from 466.0 to 2605.6 ng/g dry weight with a mean concentration of 1363.2 ng/g. Sediment samples with the highest PAH concentrations were from the upper reaches of the river, where Zhengzhou City is located; the PAH levels in the middle and lower reaches were relatively low. According to the observed molecular indices, PAHs originated largely from the high-temperature pyrolytic process. According to the numerical effect-based sediment quality guidelines (SQGs) of the United States, the levels of PAHs in the Jialu River should not exert adverse biological effects. The total benzo[a]pyrene toxicity equivalent (TEQ) values calculated for samples varied from 50.4 to 312.8 ng/g dry weight with an average of 167.4 ng/g. The relationships between PAHs and environmental factors, including chemical properties of sediments, water quality, aquatic organisms, hydrological conditions, and anthropogenic activities, are also discussed. PAHs exerted a potential negative impact on the benthos. Settlement percentage, population density and industrial GDP per capita had a significant influence on the distribution of PAHs.

Polycyclic aromatic hydrocarbons and ecotoxicological characterization of sediments from the Huaihe River, China.

The distribution, source, ecological risk and ecotoxicity of polycyclic aromatic hydrocarbons (PAHs) of sediments from 7 sampling sites, named as Xinyang (XY), Huainan (HN), Bengbu (BB), Xuyi (XuY), Fuyang (FY), Mengcheng (MC) and Zhengzhou (ZZ), in the Huaihe River basin, China, have been investigated. The total concentrations of 16 USEPA priority PAHs ranged from 62.9 to 2232.4 ng g⁻¹ dry weight (d.w.) with a mean concentration of 1056.8 ng g⁻¹ d.w. Through the assessment of ecological risk, we found that the levels of PAHs in the Huaihe River should not exert adverse biological effects. The total benzo[a]pyrene toxicity equivalent (TEQ) values calculated for samples varied from 0.01 to 194.1 ng g⁻¹ d.w., with an average of 65.9 ng g⁻¹. The toxicity data were accordant with the chemical analysis results in this study. HN, BB and ZZ showed the greatest pollution extent both in the chemical analysis and the study of ecotoxicological effects.

Synthesis, biological evaluation and molecular docking studies of amide-coupled benzoic nitrogen mustard derivatives as potential antitumor agents.

A series of amide-coupled benzoic nitrogen mustard derivatives as potential EGFR and HER-2 kinase inhibitors were synthesized and reported for the first time. Some of them exhibited significant EGFR and HER-2 inhibitory activity. Of all the studied compounds, compounds 5b and 5t exhibited the most potent inhibitory activity, which was comparable to the positive control erlotinib. Docking simulation was performed to position compounds 5b and 5t into the EGFR active site to determine the probable binding model. Antiproliferative assay results indicated that some of the benzoic nitrogen mustard derivatives possessed high antiproliferative activity against MCF-7. In particular, compounds 5b and 5t with potent inhibitory activity in tumor growth inhibition may function as potential antitumor agents.

Photo-degradation of C.I. Disperse Blue 56 by UV irradiation combined with manganese minerals.

Photo-degradation of C.I. Disperse Blue 56 (DB56) by UV irradiation combined with manganese minerals (UV/MM) was investigated in this paper. Through comparative study with the UV/titanium dioxide (TiO2) process, it was found that the UV/MM method had similar photodegradation efficiency to UV/TiO2 and was slightly more effective in removing the toxicity. In the conditions of DB56 concentration = 400 mg/L, pH = 9.0, manganese minerals dosage = 20 g/L, room temperature (25 degrees C), dissolved oxygen (O2) = 50 L/h, and reaction time = 1 hour, the color and chemical oxygen demand removal percentages were up to approximately 94 and 51%, respectively. The results of this study indicate that UV/MM is an alternative approach that should be explored further in the treatment of dyeing wastewaters.

Degradation of Active Brilliant Red X-3B by a microwave discharge electrodeless lamp in the presence of activated carbon.

Degradation of Active Brilliant Red X-3B (X-3B) in aqueous solution by a microwave discharge electrodeless lamp (MDEL) in the presence of activated carbon was investigated. The preliminary results proved this method could effectively degrade X-3B in aqueous solution. The removal percentages of colour and chemical oxygen demand were up to approximately 99% and 66%, respectively, at the conditions of 0.8 g/L dye concentration, 20 g/L activated carbon, pH 7.0 and 8 min microwave irradiation time. The degradation basically belonged to first-order reaction kinetics and its rate constant was 0.42 min(-1). No aromatic organics were detected in the final treated solution, indicating that the mineralization was relatively complete. By studying the change in solution properties, it could be concluded that MDEL-assisted oxidation was the dominant reaction mechanism. In addition, the influence of operational parameters and reuse of activated carbon were also discussed.

Treatment of simulated wastewater containing Reactive Red 195 by zero-valent iron/activated carbon combined with microwave discharge electrodeless lamp/sodium hypochlorite.

A comparative study of treatment of simulated wastewater containing Reactive Red 195 using zero-valent iron/activated carbon (ZVI/AC), microwave discharge electrodeless lamp/sodium hypochlorite (MDEL/NaClO) and the combination of ZVI/AC-MDEL/NaClO was conducted. The preliminary results showed the two steps method of ZVI/AC-MDEL/NaClO had much higher degradation efficiency than both single steps. The final color removal percentage was nearly up to 100% and the chemical oxygen demand reduction percentage was up to approximately 82%. The effects of operational parameters, including initial pH value of simulated wastewater, ZVI/AC ratio and particle size of ZVI were also investigated. In addition, from the discussion of synergistic effect between ZVI/AC and MEDL/NaClO, we found that in the ZVI/AC-MEDL/NaClO process, ZVI/AC could break the azo bond firstly and then MEDL/NaClO degraded the aromatic amine products effectively. Reversing the order would reduce the degradation efficiency.

Seasonal variation and correlation with environmental factors of photosynthesis and water use efficiency of Juglans regia and Ziziphus jujuba.

Both the photosynthetic light curves and CO(2) curves of Juglans regia L. and Ziziphus jujuba Mill. var. spinosa in three seasons were measured using a LI-6400 portable photosynthesis system. The maximal net photosynthetic rate (A(max)), apparent quantum efficiency(phi), maximal carboxylation rate (V(cmax)) and water use efficiency (WUE) of the two species were calculated based on the curves. The results showed that A(max) of J. regia reached its maximum at the late-season, while the highest values of A(max) of Z. jujuba occurred at the mid-season. The A(max) of J. regia was more affected by relative humidity (RH) of the atmosphere, while that of Z. jujuba was more affected by the air temperature. Light saturation point (LSP) and Light compensation point (LCP) of J. regia had a higher correlation with RH of the atmosphere, those of Z. jujuba, however, had a higher correlation with air temperature. V(cmax) of both J. regia and Z. jujuba had negative correlation with RH of the atmosphere. WUE of J. regia would decrease with the rise of the air temperature while that of Z. jujuba increased. Thus it could be seen that RH, temperature and soil moisture had main effect on photosynthesis and WUE of J. regia and Z. jujuba. Incorporating data on the physiological differences among tree species into forest carbon models will greatly improve our ability to predict alterations to the forest carbon budgets under various environmental scenarios such as global climate change, or with differing species composition.

Investigation of a high-pressure pressed powder pellet technique for the analysis of coal by wavelength dispersive X-ray fluorescence spectroscopy.

Using the proposed high-pressure pressed powder pellet technique, a coal sample was pressed into an ideal pellet without binders, which provides a solution to the poor self-binding quality of coal for the determination by X-ray fluorescence spectroscopy (XRF). The pellet produced by this method was more compact and smoother, which was particularly meaningful for spectrometer, where the sample is placed over the tube window. Additionally, the high-pressure sample preparation technique effectively eliminated the effect of falling powder and contamination in the Measuring chamber of the spectrometer. Wavelength Dispersive X-ray Fluorescence Spectroscopy (WDXRF) was applied successfully to the determination of carbon, nitrogen and ash content in coal samples. This could provide an alternative method for the rapid analysis of carbon, nitrogen and ash content in the coal rather than the combustion method or the high-frequency infrared absorption with a slow ashing method. Furthermore, WDXRF could provide simultaneous determination of other major, minor and trace elements by X-ray fluorescence spectroscopy. The XRF results indicated that the sensitivity, precision, and limit of detection for most components were improved when the coal sample was pressed at 1600kN compared with preparation at 400kN.

Impact of multiple bird partners on the seed dispersal effectiveness of China's relic trees.

Frugivorous birds generally exhibit an unequal contribution to dispersal effectiveness of plant species as a function of their habitat adaptation and body size. In our study, we compared the effectiveness of multiple bird species that contribute to the dispersal of the endangered relic Chinese yew, Taxus chinensis. Seven bird species dispersed T. chinensis seeds, with Picus canus, Turdus hortulorum, and Urocissa erythrorhyncha being the main dispersers. The quantity part of dispersal effectiveness was strongly influenced by two inherent characteristics of disperser species: body size and habitat adaptation. However, the quality part of dispersal effectiveness was only influenced by disperser type. For instance, small generalist birds and large specialist birds removed more seeds than other type dispersers. Moreover, small birds and specialist birds contributed slightly more to the dispersal quality of T. chinensis than large birds and generalist birds respectively; however, these differences were not significant. Our results suggest that dispersal effectiveness is affected by variety in the body size and habitat adaptation of different dispersers. Therefore, such variation should be incorporated into spatial and temporal management actions of relic plant species in patchy, human-disturbed habitats.

[Water purification of four aquatic plant species with the presence of iron-carbon interior electrolytic substrates.] / 铁-ç¢³å† ç”µè§£è´¨ä¸‹4种水生植物的净水效果.

To compare water purification of common aquatic plants in wetland ecosystem, four common aquatic plant species (Eichhornia crassipes, Nymphaea tetragona, Acorus calamus and Phragmites australis) were used as study species. The effect of aquatic plants on the change of sewage ammonium nitrogen, COD, TP content during different experimental time (1-5 d) with the presence of iron-carbon interior electrolytic substrates were analyzed in small scale experimental apparatus. The results showed that single and combined aquatic plants could effectively remove ammonium nitrogen, COD, TP from the sewage compared with the no-plant control group, but the efficiencies were significantly different among the different species. Ammonium nitrogen removal up to 100% was achieved with E. crassipes in two days, and A. calamus in three days. Each plant combination performed well on ammonium nitrogen removal. Concentration of the sewage COD approached zero with E. crassipes in three days, A. calamus performed secondly. The concentration of COD in combination of E. crassipes and A. calamus water decreased by 85.1% to a minimum of 4.33 mg·L-1. The concentration of TP was the lowest with E. crassipes in four days, and second with P. australis. The lowest concentration of TP was found with the combination of E. crassipes and A. calamusin two days. The combination effect of interior electrolyte and plant was better than that of pure interior electrolyte on the purification of sewage. Plant configuration should be optimized according to the level of pollutants.

Buddlin, a new compound from Buddleja asiatica.

A new compound, named buddlin (1), was isolated from the whole plant of Buddleja asiatica. Its structure was elucidated from spectral evidence.

Differential contribution of frugivorous birds to dispersal patterns of the endangered Chinese yew (Taxus chinensis).

The contribution of forest generalists and specialists to the dispersal pattern of tree species is not well understood. Specialists are considered low-quality dispersers because their dispersal distance is often short. However, disregard for seed deposition site may result in underestimation of the dispersal quality of specialists. The present study estimated the contribution of generalist and specialist species to the dispersal patterns of the endangered Chinese yew (Taxus chinensis) in a subtropical patchy forest in Southeast China. A relatively diverse assemblage of frugivorous birds visited T. chinensis source trees, and specialist Hypsipetes leucocephalus and generalist Urocissa erythrorhyncha were by far the highest-quantity dispersers. Considering dispersal effectiveness, the quantity aspect of effectiveness differed between the specialist assemblage and generalist assemblage; the contribution of specialists to the quantity part of effectiveness was significantly higher than that of generalists despite the relatively low diversity of specialists. After foraging, both specialist H. leucocephalus and generalist U. erythrorhyncha significantly contributed to the number of seedlings, and their contributions to seedling recruitment did not differ with regard to quality. Our results highlight the ability of T. chinensis to recruit an effective disperser assemblage in patchy habitats, thus increasing its persistence in this disturbed habitat.

Species and biogeochemical cycles of organic phosphorus in sediments from a river with different aquatic plants located in Huaihe River Watershed, China.

The results of phosphorus fractionation in the sediments from a contaminated river containing different aquatic plants, analyzed by solution 31P-NMR for Organic Phosphorus, showed that the concentration of Inorganic Phosphorus dominated in all species and Organic Phosphorus accounted for over 20% of Total Phosphorus. In general, orthophosphate was dominant in all the sampling sites. The proportion of Organic Phosphorus accounting for the Total Phosphorus in the sediments with different plant decreased in the following order: Paspalum distichum>Typha orientalis>Hydrilla verticillata. Phosphorus-accumulation ability of Paspalum distichum was obviously stronger than Typha orientalis and Hydrilla verticillata. The Organic Phosphorus was in aquatic plants dominated by humic-associated P (Hu-P), which converted to Inorganic Ohosphorus more significantly in submerged plants than in emerged plants. The sediment dominated by Paspalum distichum abundantly accumulated Organic Phosphorus in the orthophosphate monoester fraction. The degradation and mineralization of orthophosphate monoester was the important source of high Inorganic Phosphorus concentration and net primary productivity in Suoxu River. The Organic Phosphorus derived from Typha orientalis and Hydrilla verticillata was dramatically converted to Inorganic Phosphorus when the environmental factors varied.

Seasonal, spatial, and interspecific variation in quercetin in Apocynum venetum and Poacynum hendersonii, Chinese traditional herbal teas.

Quercetin is of particular importance as it has been found to have functions of suppressing tumors, reducing blood pressure, and scavenging free radicals. It is one of the major flavonoids in Apocynum venetum and Poacynum hendersonii, whose leaves have long been used as traditional herbal teas in China and Japan. Both species are also cultivated as fiber plants because of their outstanding quality of phloem fiber in stems. To obtain high output of both quercetin and fiber, it is necessary to optimize harvesting time for their leaves. Thus, understanding the developmental patterns of quercetin in leaves and fiber in stems is crucial to achieving this goal. In the present study, temporal and interspecific variations in quercetin in the leaves between A. venetum and P. hendersonii and spatial variation among P. hendersonii populations were studied by HPLC during the period from April to October in 1999. The results show that the content of quercetin in both species reached its highest level in summer and its lowest in autumn. The quercetin content in the leaves of P. hendersonii was generally higher than that of A. venetum no matter when their leaves were harvested. There was significant difference in quercetin content among three geographical populations of P. hendersonii, which might be the result of climatic difference-cooler climate might favor accumulation of quercetin in the leaves of P. hendersonii. Furthermore, the developmental patterns of total phenolics in the leaves of the two species were the same as that of the quercetin, that is, summer is an optimal harvesting season for both quercetin and other phenolics. The results obtained here suggest that P. herdersonii is a better material for herbal tea or pharmaceutical purposes, and that the best harvest time of its leaves should be summer.

Sediment type affects competition between a native and an exotic species in coastal China.

Different types of sediments in salt marsh have different physical and chemical characters. Thus sediment type plays a role in plant competition and growth in salt marsh ecosystems. Spartina anglica populations have been increasingly confined to upper elevation gradients of clay, and the niche sediment has changed. Because the niches of S. anglica and the native species Scirpus triqueter overlap, we conducted a greenhouse experiment to test the hypothesis that plant competition has changed under different types of sediments. Biomass and asexual reproduction were analyzed, and inter- and intraspecific competition was measured by log response ratio for the two species in both monoculture and combination under three sediment types (sand, clay and mixture of sand and clay). For S. anglica, biomass, ramet number and rhizome length in combination declined significantly compared with those in monoculture, and the intensity of interspecific competition was significantly higher than that of intraspecific competition under all sediments. For S. triqueter, the intensities of intra- and interspecific competition were not significantly different. This indicates that S. triqueter exerts an asymmetric competitive advantage over S. anglica across all sediments, but especially clay. Thus the sediment type changes competition between S. anglica and S. triqueter.

[Microbial biomass and its correlations with carbon, nitrogen, and phosphorus in the sediments of Taihu Lake].

To explore the responses and feedbacks of the microbes in the sediments of Taihu Lake to the sediment nutrients, an investigation was made on the microbial biomass carbon (MB(C)), microbial biomass nitrogen (MB(N)), microbial biomass phosphorus (MB(P)), and their correlations with the total organic carbon (TOC), total nitrogen (TN), and total phosphorus (TP) in the sediments. The microbial biomass in the sediments was 184.66 mg x kg(-1), being higher at the lakeside than in the mid-lake region. The MB(C) was higher in the western coastal region, Zhushan Bay, and Meiliang Bay, with an average of 127.57 mg x kg(-1), MB(N) was higher in Meiliang Bay, Gonghu Bay, mid-lake region close to Meiliang Bay and Gonghu Bay, and eastern costal region, with an average of 19.25 mg x kg(-1), and MB(P) was higher in the eastern region and parts of the mid-lake region, with an average was 19.09 mg x kg(-1). The TOC high value zone (> or = 2.30 g x kg(-1)) was mainly in Zhushan Bay, western coastal region, Meiliang Bay, and Gonghu Bay, with an average of 1.59 g x kg(-1), TN high value zone (> or = 0.30 g x kg(-1)) was mainly in the Gonghu Bay, Meiliang Bay, Zhushan Bay, and western costal region, with an average of 0.21 g x kg(-1), and TP high value zone (> or = 1.20 g x kg(-1)) was mainly in the eastern coastal region and parts of the mid-lake region, with an average of 0.55 g x kg(-1). The TOC/TN ratio in the sediments was 7-19, with an average of 8.97, which showed that the organic substances in the sediments had obvious dual sources, among which, terrestrial organisms were mainly in the west side of the lake. The microbial biomass in the sediments was significantly positively correlated with sediment TOC and TN but had less correlation with sediment TP, and the MB(C)/MB(N) was significantly correlated with sediment TOC/TN, suggesting that the microbes in the sediments of Taihu Lake were mainly affected by the sediment TOC and TN, and the changes of the TOC/TN had significant effects on the microbial community structure.