[Histological research on the effect of viruses on wound healing]. / Histologische Untersuchungen über den Einfluss von Viren auf die Wundheilung

The influence of pure virus solution on wound healing was studied in animals. The rate of disturbed wound healing was proportional to the concentration of the virus solution. Severe necrosis without inflammatory reaction could be demonstrated histologically.

[The effect of various suture materials on wound healing. An experimental study in animals (author's transl)]. / Einfluss von unterschiedlichem Nahtmaterial auf die Wundheilung. Eine tierexperimentelle Studie.

The reactions of connective tissue and fibers to silk, catgut, chromic catgut and polyglucolic acid fibers buried in the subcutis and to which a solution of vaccinia (WHO Elstree strain) or a control solution was added, were tested in 7 rabbits. Of the fibers tested, the histological picture on the 9th postoperative day showed that polygluconic acid fibers were by far the best, catgut fibers were particularly poor.

[Iron absorption after gastrectomy following oral application of tardyferon]. / Eisenresorption nach Gastrektomie unter Tardyferon-Medikation.

Iron absorption was studied in 35 volunteers following oral application of Tardyferon during 21 days. In 25 patients a total gastrectomy and in 5 patients a subtotal gastrectomy have been performed, whereas 5 persons served as controls. The following parameters have been determined: Serum iron, iron binding capacity, hemoglobin, hematocrit, red blood cells and reticulocytes. The following results were obtained: Serum iron is markedly reduced in the patients with total gastrectomy during the first postoperative year, increases steadily and reaches normal values after the third postoperative year. Following oral application of Tardyferon an improved absorption is observed during the postoperative course. Because of the excellent absorption of Tardyferon it can be recommended for the oral application during the first two postoperative years.

[Studies of gastric motility following SPV and pyloroplasty in dogs (author's transl)]. / Veränderung der Magenmotilität durch SPV mit Pyloroplastik-Untersuchungen am Hundemagen.

UNLABELLED: In unanesthetized dogs, the mechanical activity of the antrum and pylorus was studied by means of strain gauge force transducers during fasting condition, after feeding, and following application of metoclopramide (Paspertin), before and after SPV without pyloroplasty, and after SPV with pyloroplasty respectively. RESULTS: 1) The mechanical activity during fasting condition differs from that after feeding. 2) During fasting, the contractile force is decreased for about 15%-20% after SPV without pyloroplasty and for about 50% after SPV with pyloroplasty. The frequency remains unchanged. 3) After feeding, the contractile force is decreased by about 50%, the frequency shows an increase of 100%. The same results can be obtained after SPV with or without pyloroplasty. 4) After metoclopramide the contractile force is increased up to 30%, during fasting, after feeding, and also before and after SPV. The frequency, however, shows no significant change. 5) The postoperative findings remained unchanged throughout the entire observation period (up to the 20th postoperative month).

[The noninvasive investigation of duodenogastric reflux before and after pyloroplasty (author's transl)]. / Experimentelle Untersuchungen zur nichtinvasiven Messung des duodenogastralen Refluxes vor und nach Pyloroplastik.

In five anesthetized cats the excretion of Tc-99m-DAIDA into the gallbladder and the small bowel and the d.g. reflux were visualized by a gamma camera and analyzed by a digital computer. A d.g. reflux index was calculated following stimulation of the gallbladder by ceruletide and test meals, before and after a Heineke-Mikulicz pyloroplasty. It could be demonstrated that tracing of bile with TC-99m-DAIDA can be used as a noninvasive method for the evaluation of the d.g. reflux. Gastric intubation increases the d.g. reflux rate. Ceruletide is a much more effective choleretic agent than test meals. Despite a wide pyloroplasty neither an augmented d.g. reflux nor gastritis was seen, probably due to the preserved antral innervation.

[Effect of various vagal functional conditions on the blood flow of abdominal organs]. / Der Einfluss verschiedener vagaler Funktionszustände auf die Durchblutung der Bauchorgane.

In 21 mongrel dogs blood flow was measured in different gastrointestinal organs under fasting conditions, vagal stimulation with 2-desoxy-D-glucose and vagotomy. The examinations were performed in anaesthesia with the microsphere method. Basal blood flow was found in corpus, fundus and antrum below 0,5 ml/g X min, whereas in the region of the small curvature values of 0,7 ml/g X min were observed. Vagotomy decreases flowrates especially in the mucosa of the stomach except in the region of the antrum. There a significant increase was measured. The stimulated bloodflow shows a similar effect by a vagotomy, but on an elevated lovel. Observations over the time of 3 weeks revealed that bloodflow changes due to vagotomy are not long lasting over that time. Only a decrease can be observed in the region of the antrum. Also in the other abdominal organs bloodflow changes due to vagotomy are terminated to a short time interval. Except the bloodflow changes in the gallbladder lasts longer than 4 weeks. From this investigation the conclusion can be drawn that vagotomy does not cause long lasting and radical bloodflow changes in the abdomen.

[Non-resecting surgery for gastroduodenal ulcer. III. Clinical results (author's transl)]. / Die nicht-resezierende Chirurgie des Gastro-Duodenal-Ulkus. III. Klinische Resultate

During the last 12 years, 1489 vagotomies were performed at the Chirurgische Poliklinik of Munich University; 1339 of them were selective proximal vagotomies (SPV). The main indication was duodenal ulcer (n = 915) and gastric ulcer (n = 188), including emergency operations for extensive bleeding. Further indications were: sliding hiatal hernia, erosive gastritis and achalasia of the cardia. The SPA was combined in all cases with a pyloroplasty based on form and function. The results are shown in detail related to mortality (elective 0.5%), recurrency (1.6%) and functional results (good 88.2%, fair 7.2%, poor 4.6%). The combined operation of SPV with pyloroplasty is, in our opinion, an operative procedure which allows non resectioning surgical treatment of GDU without selection, i.e. based on form and function.

Fracture healing under factor XIII medication.

Following application of factor XIII for 3 weeks bone healing was studied in Sprague-Dawley rats on the 20th postoperatively day qualitatively (radiographs, scintigraphy) and quantitatively (determination of callus thickness, of bone mineral content, and scintillation counting). No significant difference could be detected between animals treated with factor XIII and animals which served as controls. Therefore, a stimulating effect of factor XIII upon bone healing can not be expected in case of a normal factor XIII serum-level. The application of factor XIII is indicated only in high risk patients with a low factor XIII serum-level.

Are bloodflow changes after selective proximal vagotomy responsible for a necrosis in the gastric wall?

Animal experiments were performed to investigate bloodflow distribution before and after selective vagotomy to explain gastric mucosa necrosis found in some patients after vogtomy. Flow measurements were made using the microsphere technique. Changes in the microcirculation were determined under basal and stimulated conditions (250 mg 2-desoxy-D-glucose). After selective vagotomy a reduction in bloodflow of up to 50% could be observed in all layers of the stomach. There were no differences in the bloodflow along either the lesser or greater curvature under basal conditions. In the stimulated stomach, however, the increase in bloodflow along the lesser curvature was higher, although the vessels of this region were mostly altered by SPV operative procedure. From these results it can be concluded that a reducton of arterial input after selective proximal vagotomy is probably compensated for by intramucosal or submucosal anastomoses. Therefore, necrosis in the gastric wall is not due to SPV but probably to other accompanying diseases.

[Inhibition of stimulated gastric secretion with simultaneous administration of cimetidine and somatostatin]. / Hemmung der stimulierten Magensekretion durch simultane Gabe von Cimetidin und Somatostatin.

In four gastric fistula dogs we studied the effect of cimetidine (5 mg/kg/h) and somatostatin (10 microgram/kg/h), alone or in combination, on gastric secretion stimulated by histamine (160 microgram/kg/h) or pentagastrin (6 microgram/kg/h). Acid and pepsin output after histamine stimulation was more reduced by cimetidine than after pentagastrin stimulation. Somatostatin showed an inhibitory effect only after pentagastrin stimulation. The most effective reduction, both on the degree of inhibition and on duration after cessation of the inhibitors, was seen after the combination of cimetidine/somatostatin. These results give some clinical aspects for the use of these two agents.

[Model for the study of influences on fracture healing. Animal experiments]. / Modell zum Studium von Einflüssen auf die Frakturheilung. Tierexperimentelle Untersuchungen.

Primary and secondary bone healing were studied for 30 and 60 days, respectively, by means of X rays, scintigraphy, determination of callus thickness, scintillation counter, and determination of bone mineral content. Primary bone healing was terminated after 30 days, secondary healing after 60 days. Scintillation counting proved to be a quantitative method, the other methods serving as controls. The different influences on bone healing can be quantitatively studied by means of this model.

[Experimental studies on the effect of thyrocalcitonin on secondary bone healing (author's transl)]. / Experimentelle Untersuchungen zum Einfluss von Calcitonin auf die sekundäre Knochenbruchheilung.

The effect of thyrocalcitonin on secondary bone healing was studied in 180 Sprague-Dawley-rats. The right tibia of all the rats was osteotomized. Half of the animals was treated with thyrocalcitonin. Until the 60th postoperative day, the increase in 99mTc-methylendiphosphonate activity was measured and significant differences in ratios were noted. Between the postoperative days 10 to 25, there was a significant difference in the impulse quotients between the thyrocalcitonin and untreated rats (P less than 0.05). These results indicate that thyrocalcitonin may aid in secondary bone healing.