Liver kinase B1 (LKB1) regulates the epigenetic landscape of mouse pancreatic beta cells.

Liver kinase B1 (LKB1/STK11) is an important regulator of pancreatic ß-cell identity and function. Elimination of Lkb1 from the ß-cell results in improved glucose-stimulated insulin secretion and is accompanied by profound changes in gene expression, including the upregulation of several neuronal genes. The mechanisms through which LKB1 controls gene expression are, at present, poorly understood. Here, we explore the impact of ß cell-selective deletion of Lkb1 on chromatin accessibility in mouse pancreatic islets. To characterize the role of LKB1 in the regulation of gene expression at the transcriptional level, we combine these data with a map of islet active transcription start sites and histone marks. We demonstrate that LKB1 elimination from ß-cells results in widespread changes in chromatin accessibility, correlating with changes in transcript levels. Changes occurred in hundreds of promoter and enhancer regions, many of which were close to neuronal genes. We reveal that dysregulated enhancers are enriched in binding motifs for transcription factors (TFs) important for ß-cell identity, such as FOXA, MAFA or RFX6, and we identify microRNAs (miRNAs) that are regulated by LKB1 at the transcriptional level. Overall, our study provides important new insights into the epigenetic mechanisms by which LKB1 regulates ß-cell identity and function.

Parental Burnout During the Second Year of the COVID-19 Pandemic: Exploring the Role of Parenting Stressors and Coparenting Support.

The COVID-19 pandemic brought about many changes in family routines and introduced new stressors for parents. While stressors can lead to parental burnout, coparenting support may mitigate the effects of parental stress on parental burnout. The current study explored the effects of parental stress, COVID-19 stress, and coparenting support on parental burnout during the second year of the pandemic. Participants consisted of one hundred fifty-five parents in the USA (M = 39.6, SD = 7.38; female = 94.8%). Results suggested parental stress was positively associated with parental burnout while coparenting support was negatively associated with parental burnout. These findings highlight the importance of addressing parental stress and support to minimize the risk of parental burnout.

A Soft Multi-Axis High Force Range Magnetic Tactile Sensor for Force Feedback in Robotic Surgical Systems.

This paper presents a multi-axis low-cost soft magnetic tactile sensor with a high force range for force feedback in robotic surgical systems. The proposed sensor is designed to fully decouple the output response for normal, shear and angular forces. The proposed sensor is fabricated using rapid prototyping techniques and utilizes Neodymium magnets embedded in an elastomer over Hall sensors such that their displacement produces a voltage change that can be used to calculate the applied force. The initial spacing between the magnets and the Hall sensors is optimized to achieve a large displacement range using finite element method (FEM) simulations. The experimental characterization of the proposed sensor is performed for applied force in normal, shear and 45° angular direction. The force sensitivity of the proposed sensor in normal, shear and angular directions is 16 mV/N, 30 mV/N and 81 mV/N, respectively, with minimum mechanical crosstalk. The force range for the normal, shear and angular direction is obtained as 0-20 N, 0-3.5 N and 0-1.5 N, respectively. The proposed sensor shows a perfectly linear behavior and a low hysteresis error of 8.3%, making it suitable for tactile sensing and biomedical applications. The effect of the material properties of the elastomer on force ranges and sensitivity values of the proposed sensor is also discussed.

Integration of ZnO nanorods with MOS capacitor for self-powered force sensors and nanogenerators.

In this work, we present a novel force-sensing device with zinc oxide nanorods (ZnO NRs) integrated with a metal-oxide-semiconductor (MOS) capacitor and encapsulated with Kapton tape. The details of the fabrication process and working principle of the integrated ZnO NRs-MOS capacitor as a force sensor and nanogenerator have been discussed. The fabricated ZnO-MOS device is tested for both the open-circuit and resistor-connected mode. For an input force in the range of 1-32 N, the open-circuit output voltage of the device is measured to be in the range of 60-100 mV for different device configurations. In the resistor-connected mode, the maximum output power of 0.6 pW is obtained with a 1 MΩ external resistor and input force of 8 N. In addition, the influence of different seed layers (Ag and ZnO) and the patterning geometry of the ZnO nanorods on the output voltage of ZnO-MOS device have been investigated by experiments. An equivalent circuit model of the device has been developed to study the influence of the geometry of ZnO NRs and Kapton tape on the ZnO-MOS device voltage output. This study could be an example of integrating piezoelectric nanomaterials on traditional electronic devices and could inspire novel designs and fabrication methods for nanoscale self-powered force sensors and nanogenerators.

Understanding adolescents' perceived social responsibility: The role of family cohesion, interdependent self-construal, and social trust.

INTRODUCTION: Positive family interactions contribute to the development of social responsibility among adolescents. Interdependent self-construal and social trust, which reflect the perceived relatedness and beliefs towards others, may explain the relation between family cohesion and social responsibility. The present study tested the mediating mechanisms between family cohesion and adolescents' social responsibility via adolescents' interdependent self-construal and social trust. METHODS: A total of 386 Chinese children in Hong Kong (52.07% girls, Mage = 13.64 years) and their parents completed self-report questionnaires twice at 12 months apart. Family cohesion was measured by mothers', fathers', and adolescents' reports to provide a comprehensive representation of the family environment. A structural equation modeling was conducted to investigate the mediation effect. RESULTS: Findings based on structural equation modeling revealed that family cohesion was positively associated with interdependent self-construal and social trust. In addition, adolescents' interdependent self-construal and social trust were positively associated with social responsibility. Bootstrapping analysis showed that interdependent self-construal and social trust were mediators between family cohesion and social responsibility. CONCLUSION: Based on these findings, the study added new evidence to the literature by demonstrating the mediating role of interdependent self-construal and social trust between family cohesion and social responsibility. Future studies could examine potential cultural variabilities in Western and other Chinese contexts.

PDMS-ZnO Piezoelectric Nanocomposites for Pressure Sensors.

The addition of piezoelectric zinc oxide (ZnO) fillers into a flexible polymer matrix has emerged as potential piezocomposite materials that can be used for applications such as energy harvesters and pressure sensors. A simple approach for the fabrication of PDMS-ZnO piezoelectric nanocomposites based on two ZnO fillers: nanoparticles (NP) and nanoflowers (NF) is presented in this paper. The effect of the ZnO fillers' geometry and size on the thermal, mechanical and piezoelectric properties is discussed. The sensors were fabricated in a sandwich-like structure using aluminium (Al) thin films as top and bottom electrodes. Piezocomposites at a concentration of 10% w/w showed good flexibility, generating a piezoelectric response under compression force. The NF piezocomposites showed the highest piezoelectric response compared to the NP piezocomposites due to their geometric connectivity. The piezoelectric compound NF generated 4.2 V while the NP generated 1.86 V under around 36 kPa pressure. The data also show that the generated voltage increases with increasing applied force regardless of the type of filler.

Emotion dysregulation between mothers, fathers, and adolescents: Implications for adolescents' internalizing problems.

INTRODUCTION: The present study calls attention to the longitudinal relations between mothers', fathers', and adolescents' emotion dysregulation and adolescents' internalizing problems. To this end, we tested the associations between family members' emotion dysregulation and adolescents' internalizing problems over time. METHODS: Over a 12-month period, 386 Chinese families from Hong Kong involving mothers, fathers, and adolescent children (children at 12-17 years of age; boys = 185, girls = 201) completed a set of questionnaires twice. RESULTS: Multi-group path analysis revealed unidirectional effects of mothers' emotion dysregulation on fathers' and adolescents' emotion dysregulation over time. Adolescents' emotion dysregulation was also related to their subsequent internalizing problems. The associations did not differ as a function of adolescents' gender. CONCLUSION: The present findings underscore the significance of mothers' emotion dysregulation on fathers' and adolescents' emotion dysregulation. As a risk factor, adolescents' emotion dysregulation was also predictive of their internalizing problems 12 months later. Taken together, this study serves to inform prevention and intervention efforts in promoting emotion regulation as a family asset associated with fewer adolescents' internalizing problems.

Why does acculturative stress elevate depressive symptoms? A longitudinal study with emotion regulation as a mediator.

Acculturative stress has repeatedly been shown to heighten depressive symptoms. However, the explanatory process between acculturative stress and depressive symptoms has been understudied, particularly in the Chinese context. This prospective study aims to investigate emotion regulation difficulties as a mechanism between acculturative stress and depressive symptoms. A sample of 154 Mainland Chinese female university students (Mage = 21.10; SD = 2.13) were recruited in Hong Kong 3 times, with a 4-month lag between assessment points. Mediation findings converged to suggest between-person indirect effect of acculturative stress on symptoms of depression via emotion regulation difficulties, above and beyond established mediators including self-stigma and social support. Specifically, greater acculturative stress was related to a higher level of emotion regulation difficulties. In turn, greater emotion regulation difficulties were related to a higher level of depressive symptoms. The stress-generating effect of depression via emotion regulation was not simultaneously supported. These findings are informative to researchers and practitioners aiming to alleviate depressive symptoms, particularly in the Chinese context of higher education that often involves intercultural contact and stress. (PsycInfo Database Record (c) 2020 APA, all rights reserved).

The transcription factor Pax6 is required for pancreatic ß cell identity, glucose-regulated ATP synthesis, and Ca2+ dynamics in adult mice.

Heterozygous mutations in the human paired box gene PAX6 lead to impaired glucose tolerance. Although embryonic deletion of the Pax6 gene in mice leads to loss of most pancreatic islet cell types, the functional consequences of Pax6 loss in adults are poorly defined. Here we developed a mouse line in which Pax6 was selectively inactivated in ß cells by crossing animals with floxed Pax6 alleles to mice expressing the inducible Pdx1CreERT transgene. Pax6 deficiency, achieved by tamoxifen injection, caused progressive hyperglycemia. Although ß cell mass was preserved 8 days post-injection, total insulin content and insulin:chromogranin A immunoreactivity were reduced by ∼60%, and glucose-stimulated insulin secretion was eliminated. RNA sequencing and quantitative real-time PCR analyses revealed that, although the expression of key ß cell genes, including Ins2, Slc30a8, MafA, Slc2a2, G6pc2, and Glp1r, was reduced after Pax6 deletion, that of several genes that are usually selectively repressed ("disallowed") in ß cells, including Slc16a1, was increased. Assessed in intact islets, glucose-induced ATP:ADP increases were significantly reduced (p < 0.05) in ßPax6KO versus control ß cells, and the former displayed attenuated increases in cytosolic Ca2+ Unexpectedly, glucose-induced increases in intercellular connectivity were enhanced after Pax6 deletion, consistent with increases in the expression of the glucose sensor glucokinase, but decreases in that of two transcription factors usually expressed in fully differentiated ß-cells, Pdx1 and Nkx6.1, were observed in islet "hub" cells. These results indicate that Pax6 is required for the functional identity of adult ß cells. Furthermore, deficiencies in ß cell glucose sensing are likely to contribute to defective insulin secretion in human carriers of PAX6 mutations.

Trivariate Modeling of Interparental Conflict and Adolescent Emotional Security: An Examination of Mother-Father-Child Dynamics.

Recognizing the significance of interacting family subsystems, the present study addresses how interparental conflict is linked to adolescent emotional security as a function of parental gender. A total of 272 families with a child at 12.60 years of age (133 boys, 139 girls) were invited to participate each year for three consecutive years. A multi-informant method was used, along with trivariate models to test the associations among mothers, fathers, and their adolescent children's behaviors. The findings from separate models of destructive and constructive interparental conflict revealed intricate linkages among family members. In the model of destructive interparental conflict, mothers and fathers predicted each other's conflict behaviors over time. Moreover, adolescents' exposure to negativity expressed by either parent dampened their emotional security. Consistent with child effects models, adolescent emotional insecurity predicted fathers' destructive conflict behaviors. As for the model of constructive interparental conflict, fathers predicted mothers' conflict behaviors over time. Adolescents' exposure to fathers' constructive conflict behaviors also enhanced their sense of emotional security. Consistent with child effects models, adolescent emotional security predicted mothers' and fathers' constructive conflict behaviors. These findings extended the family and the adolescent literature by indicating that family processes are multiidirectional, involving multiple dyads in the study of parents' and adolescents' functioning. Contributions of these findings to the understanding of interparental conflict and emotional security in adolescence are discussed.

Vasostatin-1 antigenic epitope mapping for induction of cellular and humoral immune responses to chromogranin A autoantigen in NOD mice.

The chromogranin A (ChgA) 29-42 sequence is the antigenic epitope for the BDC2.5 CD4(+) T-cell receptor in NOD mice (H-2(g7) ). We have now characterized the binding register of the ChgA 29-42 peptide for the I-A(g7) molecule. Truncation of the peptide demonstrated that the KCVLEVISD sequence 34-42 is the binding register and extension of this sequence by flanking residues increased its binding affinity and antigenic capacity. We employed anti-ChgA peptide antibodies generated against different fragments of ChgA for immunostaining of pancreatic islet sections from NOD mice. A strong immuno-staining pattern was observed for the ChgA 17-38 peptide antibodies that overlap with the ChgA 29-42 sequence. Moreover, sera from diabetic NOD mice showed elevated titers of autoantibodies to the ChgA 29-42 peptide. These findings indicate that peptides from the N-terminal region of ChgA are able to induce cellular and humoral immune responses in NOD mice.

Self-compassion and grit mediated the relation between mindfulness and mind wandering based on cross-sectional survey data.

Previous research suggests that mindfulness and mind wandering are opposing constructs. However, little is known about why and how they are negatively related. Through a process-oriented approach, this cross-sectional study tested self-compassion and grit as mediators for the relation between mindfulness and mind wandering. A total of 487 self-identified meditators were recruited from the UK (241 female, 49.49%). Participants reported a mean age of 38.98 years (SD = 10.03), with an average of 2.26 h of meditation practice per week (SD = 4.47). Upon informed consent, the participants completed a self-report questionnaire that assessed the core variables under study. Path analysis indicated that mindfulness was related to self-compassion. Greater self-compassion was, in turn, related to greater grit, which was then related to lower mind wandering. Bootstrapping analysis further indicated that self-compassion and grit as mediators between mindfulness and mind wandering, above and beyond age, gender, hours of meditation, income, and education as covariates. The cross-sectional findings provided initial evidence of mediation by showing that mindfulness and mind wandering were related through self-compassion and grit.

Social-Emotional Competence among School-Aged Children in the Chinese Context: Validation of the Washoe County School District Social-Emotional Competency Assessment.

The present study aims to validate the 40-item and the brief 17-item Washoe County School District Social-Emotional Competency Assessment (WCSD-SECA), a self-report measure of social-emotional competencies, among Chinese school-aged children residing in Hong Kong. A total of 349 children (M = 9.86 years, SD = 1.22; 45.82% girls) and their parents (77.84% mothers) completed a set of questionnaires independently. The factor structure of both versions of the WCSD-SECA was assessed via confirmatory factor analyses. Structural equation models were then conducted to examine the predictive validity of the WCSD-SECA. The findings indicated that both the 40-item and the 17-item versions of the WCSD-SECA fit the data adequately. Both versions were also associated with self-reported positive and negative affect and parent-reported internalizing problems and externalizing problems. Additionally, social-emotional competencies as measured by the 40-item version were significantly associated with parent-reported prosocial behavior, whereas those as measured by the 17-item version were associated with prosocial behavior with a marginal significance. The findings demonstrated an adequate factor structure and predictive validity of the full version and the brief version of the WCSD-SECA in assessing social-emotional competencies. Hence, they serve as a useful tool for researchers, educators, and mental health practitioners to evaluate school-aged children's social-emotional competencies in the Chinese context.

The role of family conflict and cohesion in adolescents' social responsibility: Emotion regulation ability as a mediator.

The social context is crucial for the adolescent development of self-regulatory skills and social responsibility. To understand the role of social context in adolescent development, the present study examined family predictors (i.e., family cohesion and conflict) of social responsibility, with emotion regulation ability as a mediating process. A total of 828 Chinese adolescents (35.6% female; mean age = 13.92 years, SD = 1.34) were recruited from major Chinese cities, including Hong Kong and Macau. Path analysis revealed that emotion regulation ability mediated the relation between family factors (i.e., family cohesion and family conflict) and social responsibility. That is, the ability to regulate emotions serves as a process between family factors and social responsibility. More specifically, family cohesion was positively associated with emotion regulation ability, whereas family conflict was negatively associated with emotion regulation ability. In turn, emotion regulation ability was positively associated with social responsibility. The results suggested that the family environment and adolescent's emotion regulation ability are important contextual and intrapersonal factors contributing to their development of social responsibility. As an implication, policymakers and practitioners might allocate resources to enrich positive family interactions and cultivate emotional competency to support adolescents' development of social responsibility.

Tailoring structural and optical properties of ZnO system through elemental Mn Doping through First-principles calculations.

In this study, band structure and optical properties of Manganese (Mn) doped ZnO are investigated adopting first-principles study calculations. It is observed that, by addition of Mn in ZnO crystal, the electrical properties like conductivity and dielectric function of material have been improved. The elastic constants for the elements are also calculated which shows that the element is stable after addition of dopant. The computational study is done on CASTEP and Material Studio. The ZnO system is simulated and atoms of Mn has been added replacing Zn atoms. The properties that studied are band structure and optics including conductivity, reflectivity, dielectric function, absorption and refractive index. Furthermore, this study also includes calculation of Elastic constants, XRD Spectra, Phonon dispersion and Temperature profile of doped ZnO systems. The computational study produced promising results and experimental approach can be adopted to reinforce the outcomes of this study.

Parental self-control facilitates adolescent psychological adjustment sequentially through parents' perceived stress/mindful parenting and adolescent self-control.

Adolescence is a unique developmental period marked with significant changes and challenges. As such, maintaining optimal psychological adjustment is crucial for young people, especially during the COVID-19 pandemic when their adjustment became more challenging. Self-control is a vital ability assisting individuals to navigate difficulties and stay well-adjusted during turbulent times. While the associations between adolescent self-control and adjustment have been well-documented, parental self-control has been considered to play a more fundamental role in adolescent adjustment. However, this consideration has received scant research. Drawing on the intergenerational transmission model of self-regulation, we examined an understudied yet plausible idea that parental self-control facilitates adolescent adjustment through parents' lower levels of perceived stress/better mindful parenting and adolescents' improved self-control. A two-wave survey study, spanning 1 year apart, was conducted among 426 Chinese adolescents (Mage = 11.6 years, 53.5% boys) and their parents. Parents rated their self-control, perceived stress, and mindful parenting at T1, while adolescents rated their self-control and adjustment (i.e., psychological difficulties and life satisfaction) at T1 and T2. The results of chain mediation model showed that after controlling for demographic covariates and baseline levels of adolescent self-control and adjustment, T1 paternal self-control facilitated T2 adolescent adjustment through fathers' lower levels of perceived stress and adolescents' improved self-control. By contrast, T1 maternal self-control facilitated T2 adolescent adjustment through mothers' better mindful parenting and adolescents' improved self-control. These findings advance our understanding of how self-control is transmitted from parents to offspring and clarify the processes of how parental self-control facilitates adolescent adjustment. (PsycInfo Database Record (c) 2024 APA, all rights reserved).

Liver kinase B1 (LKB1) regulates the epigenetic landscape of mouse pancreatic beta cells.

Liver kinase B1 (LKB1/STK11) is an important regulator of pancreatic ß-cell identity and function. Elimination of Lkb1 from the ß-cell results in improved glucose-stimulated insulin secretion and is accompanied by profound changes in gene expression, including the upregulation of several neuronal genes. The mechanisms through which LKB1 controls gene expression are, at present, poorly understood. Here, we explore the impact of ß cell- selective deletion of Lkb1 on chromatin accessibility in mouse pancreatic islets. To characterize the role of LKB1 in the regulation of gene expression at the transcriptional level, we combine these data with a map of islet active transcription start sites and histone marks. We demonstrate that LKB1 elimination from ß-cells results in widespread changes in chromatin accessibility, correlating with changes in transcript levels. Changes occurred in hundreds of promoter and enhancer regions, many of which were close to neuronal genes. We reveal that dysregulated enhancers are enriched in binding motifs for transcription factors important for ß-cell identity, such as FOXA, MAFA or RFX6 and we identify microRNAs (miRNAs) that are regulated by LKB1 at the transcriptional level. Overall, our study provides important new insights into the epigenetic mechanisms by which LKB1 regulates ß-cell identity and function.

Prospective relations between parental depression, negative expressiveness, emotional insecurity, and children's internalizing symptoms.

Building on the conceptual framework of emotional security theory (Davies and Cummings in Psychol Bull 116:387-411, 1994), this study longitudinally examined multiple factors linking parental depressive symptoms and child internalizing symptoms. Participants were 235 children (106 boys, 129 girls) and their cohabiting parents. Assessments included mothers' and fathers' depressive symptoms when children were in kindergarten, parents' negative expressiveness when children were in first grade, children's emotional insecurity 1 year later, and children's internalizing symptoms in kindergarten and second grade. Findings revealed both mothers' and fathers' depressive symptoms were related to changes in children's internalizing symptoms as a function of parents' negative emotional expressiveness and children's emotional insecurity. In addition to these similar pathways, distinctive pathways as a function of parental gender were identified. Contributions are considered for understanding relations between parental depressive symptoms and children's development.

Professional support, efficacy beliefs, and compassion fatigue in principals during the COVID-19 pandemic.

Guided by the social cognitive theory and job demands-resources model, we used multiple regression analyses to examine the concurrent and interactive influences of professional support and efficacy beliefs on compassion fatigue during COVID-19 among 231 school principals in California. Controlling for principals' individual- and school-level demographic factors, professional support and their district collective efficacy (but not self-efficacy) were significantly and negatively associated with compassion fatigue. The negative association between professional support and compassion fatigue was moderated by both collective and self-efficacy. Professional support had a significantly negative association with compassion fatigue only among principals with both lower collective and self-efficacy beliefs. In addition, female principals reported significantly higher compassion fatigue than their male counterparts. Principals who oversee schools with larger student populations (i.e., 500-1,000 students) reported significantly less compassion fatigue than those who lead smaller schools (i.e., less than 200 students). Findings highlighted the importance of promoting principals' efficacy beliefs and increasing professional support to address their compassion fatigue. Findings also indicated that professional support and efficacy beliefs interact with each other in a compensatory way to influence the principal's compassion fatigue concurrently. Practical implications for school psychologists' capacity to support school leaders' compassion fatigue are discussed. (PsycInfo Database Record (c) 2023 APA, all rights reserved).

Do Bloggers Have Better Mental Health? The Social, Cognitive, and Psychological Benefits of Blogging in Emerging Adulthood.

BACKGROUND: The purpose of this study was to investigate the longitudinal processes by which blogging-related disclosure is linked to mental health. It was hypothesized that blogging had both social and cognitive benefits, including greater perceived social support and fewer memory slips, which were then associated with better mental health. METHODS: A total of 194 emerging adults were recruited three times at approximately three months apart. Participants filled out a self-report about their blogging activities and perceived benefits, social support, memory, and mental health at each time point. RESULTS: Path analysis indicated that perceived blogging-related benefits, needs, and traits mediated the relation between frequency of blogging and social support and memory slips, respectively. Moreover, social support marginally predicted greater mental health, whereas memory slips predicted poorer mental health, after controlling for baseline mental health, age, and gender. CONCLUSIONS: This study established the longitudinal associations between blogging and its benefits that may be vital for emerging adults' mental health.