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1.
Angew Chem Int Ed Engl ; 63(11): e202320036, 2024 Mar 11.
Article in English | MEDLINE | ID: mdl-38191990

ABSTRACT

The striking aesthetic appeal of fullerene-like clusters has captured the interest of researchers. Nevertheless, the assembly of fullerene-like polyoxovadanadate (POV) cages remains a significant challenge due to the scarcity of suitable pentagonal motif. Herein, we have successfully synthesized the first fullerene-like all-inorganic POV cage, {(V2 O)V30 Nb12 O102 (H2 O)12 } (V30 Nb12 ), by introducing Nb into the POVs. V30 Nb12 is assembled by 12 heterometallic {(Nb)V5 } pentagons through sharing V centers with Ih symmetry, reminiscent of C60 . To our knowledge, the fullerene-like V30 Nb12 not only represents the highest-nuclearity POV cage but also stands as the first niobovanadate cluster. Notably, V30 Nb12 exhibits excellent solution stability, as confirmed by ESI-MS, FT-IR and UV/Vis spectra. As there is no protection organic ligand on its outer surface, V30 Nb12 can be further modified with Cu-complexes to form a fullerene-like cluster based zigzag chain (Cu-V30 Nb12 ).

2.
Childs Nerv Syst ; 39(2): 553-556, 2023 02.
Article in English | MEDLINE | ID: mdl-35861856

ABSTRACT

Meningioma originating from the oculomotor nerve without dural attachment in children has been rarely reported. A 6-year-old patient presented ptosis of the right eye for 5 years. MRI indicated an occupying lesion in the right cavernous sinus. A tumor originating from the oculomotor nerve without dural attachment was found during subsequent surgery and confirmed as meningioma by pathology. Subsequently, the tumor was removed completely, and the oculomotor nerve was reconstructed using the sural nerve. The patient's symptoms were relieved partially after 3 months. The findings of this case suggested that the mechanisms underlying meningioma involve ectopic arachnoid cap cells within the nerve sheath. Thus, the tumor should be removed completely; also, nerve reconstruction is suggested.


Subject(s)
Cavernous Sinus , Meningeal Neoplasms , Meningioma , Humans , Child , Meningioma/surgery , Oculomotor Nerve/pathology , Cavernous Sinus/pathology , Neurosurgical Procedures , Meningeal Neoplasms/surgery
3.
Childs Nerv Syst ; 39(3): 671-676, 2023 03.
Article in English | MEDLINE | ID: mdl-36572815

ABSTRACT

INTRODUCTION: Type I Chiari malformation (CM-I) is a common congenital malformation, often accompanied by syringomyelia. Obstruction of CSF circulation in the foramen magnum is the reason for the formation of the syringomyelia. Fourth ventricle stenting (FVS) could ensure the CSF flow from the fourth ventricle, which might represent an effective approach to achieve syringomyelia relief. METHODS: We have reviewed four cases of CM-I children who received FVS implantation from November 2021 to July 2022. Each of the patients underwent FMD. The ventricular catheter of an Ommaya reservoir (Medtronic, Inc.) was used as a stent. Duraplasty was performed with autologous fascia. Cervical MRI rechecks were routinely done 3 months later, and the clinical symptoms were followed up. RESULTS: Four children received FVS, including one boy and three girls, aged from 12 months to 10 years, with a mean age of 6.8 years. No major complication occurred. The syringomyelia or hydrocephalus in each patient were all attenuated, as observed on the reexamination MRI images. During the follow-up time with a mean of 10.8 months, no patient had new symptoms. CONCLUSION: FVS is an effective and safe method for operation of CM-1 in children. The indications of FVS include redo-FMD, difficulties to ensure post-FMD CSF circulation patency at the foramen magnum and with obstructive hydrocephalus. A long-term follow-up duration is necessary.


Subject(s)
Arnold-Chiari Malformation , Hydrocephalus , Syringomyelia , Male , Female , Humans , Child , Syringomyelia/diagnostic imaging , Syringomyelia/surgery , Syringomyelia/complications , Fourth Ventricle/diagnostic imaging , Fourth Ventricle/surgery , Decompression, Surgical/methods , Arnold-Chiari Malformation/diagnostic imaging , Arnold-Chiari Malformation/surgery , Arnold-Chiari Malformation/complications , Magnetic Resonance Imaging/methods , Hydrocephalus/diagnostic imaging , Hydrocephalus/etiology , Hydrocephalus/surgery , Stents/adverse effects , Treatment Outcome
4.
Childs Nerv Syst ; 39(6): 1663-1666, 2023 06.
Article in English | MEDLINE | ID: mdl-36759369

ABSTRACT

PURPOSE: Spinal cord diffuse midline glioma (DMG) with H3 K27-alteration is a group of spinal cord high-grade glioma with poor outcome. We present a case with rare onset symptom pattern of pediatric spinal DMG, contributing to the understanding of the clinical presentations and natural history of pediatric spinal cord DMG. METHODS AND RESULTS: A 7-year-old boy was admitted due to symptoms of intracranial hypertension without obvious spinal cord-related symptoms. Head radiological examinations, blood and cerebral spinal fluid tests did not support intracranial lesion, infection, or autoimmune diseases. Spinal magnetic resonance imaging revealed intraspinal occupying lesion with leptomeningeal dissemination. Pathology of the lesion verified DMG with H3 K27M-alteration. CONCLUSION: Pediatric DMG with leptomeningeal dissemination could present with initial symptoms of intracranial hypertension without obvious spinal cord-related symptoms. Spinal cord examinations in cases of intracranial hypertension with negative head radiological examination results could be valuable in finding the etiology.


Subject(s)
Glioma , Intracranial Hypertension , Spinal Cord Neoplasms , Male , Humans , Child , Spinal Cord Neoplasms/complications , Spinal Cord Neoplasms/diagnostic imaging , Glioma/complications , Glioma/diagnostic imaging , Hospitalization , Intracranial Hypertension/diagnostic imaging , Intracranial Hypertension/etiology
5.
Childs Nerv Syst ; 39(5): 1109-1110, 2023 05.
Article in English | MEDLINE | ID: mdl-36917269

ABSTRACT

We made a reply to the letter to editor regarding "Fourth ventricle stent placement for treatment of type I Chiari malformation in children" by Prof. Afshari. He gave some comments on the primary cause of low-lying cerebellar tonsils and the risk of the fourth ventricle stent (FVS). We make further explanation of our opinion on Chiari malformation and the value of FVS.


Subject(s)
Arnold-Chiari Malformation , Fourth Ventricle , Male , Humans , Child , Fourth Ventricle/surgery , Arnold-Chiari Malformation/surgery , Stents
6.
Childs Nerv Syst ; 38(10): 1987-1991, 2022 10.
Article in English | MEDLINE | ID: mdl-35608661

ABSTRACT

BACKGROUND: The cavernous sinus, which has several important structures, can be affected by various lesions, including tumor, vascular, infection, and inflammation. CASE REPORT: We reported a rare case of abscess of the cavernous sinus in a child presenting with headache and abducens paralysis. Exploratory surgery was performed via the Dolenc approach, and the patient recovered from abducens paralysis 3 months later. CONCLUSION: Abscess of the cavernous sinus is rare.  Inspection of cavernous sinus can confirm the characteristics of the lesion and decompress the cavernous sinus, which may be beneficial for nerve function recovery.


Subject(s)
Abducens Nerve Diseases , Cavernous Sinus , Abducens Nerve Diseases/etiology , Abducens Nerve Diseases/pathology , Abducens Nerve Diseases/surgery , Abscess/pathology , Cavernous Sinus/diagnostic imaging , Cavernous Sinus/pathology , Cavernous Sinus/surgery , Child , Headache/complications , Humans , Paralysis
7.
Childs Nerv Syst ; 38(8): 1557-1566, 2022 08.
Article in English | MEDLINE | ID: mdl-35635570

ABSTRACT

INTRODUCTION: Spontaneous spinal epidural hematoma (SSEH) is a rare neurosurgical emergency, presenting as sudden onset of back pain and weakness of lower extremities. Many patients have no definite cause. Some cases of SSEH caused by vascular malformation have been reported. The treatment strategy remains controversial. This study aimed to analyze the causes of SSEH and proposed a treatment strategy according to clinical outcomes of patients at a single institution. METHODS: A total of 25 cases of SSEH under 18 years of age treated between March 2004 and July 2021 were retrospectively analyzed. RESULTS: The mean age of the first SSEH onset was 7.1 years. The most common location was cervicothorax. Nine patients suffered from multiple episodes. Twenty-three patients underwent spinal digital subtraction angiography (DSA), of which seven (30.4%) patients had positive findings: three cases had epidural artery venous fistula (AVF), two cases had epidural artery venous malformation (AVM), and two cases had abnormal concentration of contrast agent. Seventeen patients received surgery. Eleven patients (44%) were diagnosed as vascular malformation by either DSA or pathology. The follow-up rate was 80%, with 20 patients (80%) achieving satisfactory clinical outcome. Risk factors for poor clinical outcome included multiple episodes (p = 0.028) and higher Aminoff-Logue score (p = 0.005). CONCLUSION: Spinal epidural vascular malformation is a significant cause of SSEH. Spinal DSA is necessary. Surgery should be recommended for patients with multiple episodes, positive findings on DSA, or severe neurological deficits. Conservation therapy can be considered for other patients, but long-time follow-up is necessary.


Subject(s)
Hematoma, Epidural, Spinal , Vascular Malformations , Adolescent , Child , Hematoma, Epidural, Spinal/diagnostic imaging , Hematoma, Epidural, Spinal/etiology , Hematoma, Epidural, Spinal/surgery , Humans , Laminectomy/adverse effects , Magnetic Resonance Imaging/adverse effects , Retrospective Studies , Vascular Malformations/complications
8.
Childs Nerv Syst ; 38(11): 2141-2148, 2022 11.
Article in English | MEDLINE | ID: mdl-36190523

ABSTRACT

OBJECTIVE: The cause and mechanism of epilepsy after endoscopic third ventriculostomy (ETV) have still remained elusive. This single-center study aimed to explore and analyze the risk factors of post-operative seizure in pediatric patients with hydrocephalus undergoing ETV. METHODS: Data of pediatric patients with hydrocephalus who were treated with ETV from October 1, 2015, to November 31, 2021, were retrospectively analyzed. Basic demographic characteristics, etiology of hydrocephalus, surgical details, and laboratory measurements were collected. An early postoperative seizure was defined as the occurrence of at least one clinical seizure within 24 h of ETV. RESULTS: A total of 50 participants were included in the study, of whom 5 (10.00%) cases were in postoperative epilepsy group and 45 (90.00%) cases were in non-epilepsy group. Epilepsy patients were younger than those without epilepsy, while no statistically significant difference was found (P = 0.0836). In the age subgroup, children with epilepsy were younger than 2 years old. All patients with epilepsy received Ringer's solution intraoperatively. The mean postoperative serum calcium and potassium concentrations were significantly lower in patients with epilepsy than in those without epilepsy (Pcalcium = 0.0429; Ppotassium = 0.0250). Moreover, a faster decrease of serum potassium and calcium levels was found in children with epilepsy compared with those without epilepsy after ETV. CONCLUSION: The decrease of serum calcium and potassium levels, younger age, and using Ringer's solution as irrigation fluid were risk factors for epilepsy after ETV.


Subject(s)
Hydrocephalus , Neuroendoscopy , Third Ventricle , Humans , Child , Infant , Child, Preschool , Ventriculostomy/adverse effects , Third Ventricle/surgery , Retrospective Studies , Calcium , Ringer's Solution , Neuroendoscopy/adverse effects , Treatment Outcome , Hydrocephalus/surgery , Hydrocephalus/etiology , Seizures/surgery , Seizures/complications , Risk Factors , Potassium
9.
Pediatr Neurosurg ; 57(6): 422-433, 2022.
Article in English | MEDLINE | ID: mdl-35973405

ABSTRACT

INTRODUCTION: Pediatric meningiomas (PMs) are rare tumors; they differ from their adult counterparts by their atypicality of location, higher rates of malignant change, male preponderance, recurrence, and sometimes, their association with neurofibromatosis. This case series analyzes the clinical behavior, pathological presentation, location, and its association with neurofibromatosis type 2 (NF2). METHODS: This case series consists of pediatric patients between the ages of 4 and 16 years who were hospitalized in the neurosurgical department of our hospital from 2012 to 2021 with different neurological symptoms and a literature review using the PubMed/MEDLINE database. RESULTS: Sixty percent of the patients were males, while 40% were females. The most common neurological manifestations were signs of increased intracranial pressure. NF2 was absent in all patients. The predominant histopathology subtypes are atypical and WHO grade II, representing 30% and 40%, respectively. CONCLUSION: This study supports the relationship between NF2 and pediatric cerebral meningioma but at a lower concomitant rate from 0 to 13%, taking into consideration our original data and the literature review, contrasting some reported cases, which suggest rates as high as 33%, 50%, and 100% in a very small number of patients. Gross total resection without postoperative radiation therapy for nonmalignant and non-NF2-associated PM proved to be a sufficient and a good treatment option.


Subject(s)
Intracranial Hypertension , Meningeal Neoplasms , Meningioma , Neurofibromatosis 2 , Adult , Female , Child , Humans , Male , Child, Preschool , Adolescent , Meningioma/diagnostic imaging , Meningioma/surgery , Neurofibromatosis 2/complications , Neurofibromatosis 2/pathology , Meningeal Neoplasms/diagnostic imaging , Meningeal Neoplasms/surgery , Intracranial Hypertension/complications
10.
Acta Biochim Biophys Sin (Shanghai) ; 50(3): 238-245, 2018 Mar 01.
Article in English | MEDLINE | ID: mdl-29385406

ABSTRACT

C-reactive protein (CRP) acts as a biomarker reflecting different degrees of inflammation. Accumulating reports have suggested that there is a close relationship between CRP and various cancers. However, the influence of CRP on the development of tongue squamous cell carcinoma (TSCC) remains unclear. The purpose of this study was to investigate the role of CRP in TSCC. The results of immunohistochemical staining and statistical analyses showed that CRP expression was associated with TSCC tumor size, lymph node metastasis and pathological differentiation. Cell Counting Kit-8 (CCK-8) assay revealed that CRP could enhance TSCC cell proliferation in a dose- and time-dependent manner. Moreover, with CRP stimulation, proliferating cell nuclear antigen (PCNA) expression patterns presented a notable time-dependent up-regulation. In addition, CRP could enhance the invasion and migration of TSCC cells, as revealed by transwell and wound-healing assays, respectively. Annexin V-FITC/PI staining showed that CRP could protect TSCC cells from starvation- and drug-induced apoptosis. With CRP stimulation, the protein expression levels of phosphorylated protein kinase B (pAkt), phosphorylated mammalian target of rapamycin (pmTOR) and phosphorylated S6 ribosomal protein (pS6) were significantly increased, as demonstrated by western blot analysis. Our data suggest that CRP may play an important role in the development of TSCC. Moreover, the biological effects of CRP on TSCC cells might be related to Akt, mTOR, and S6.


Subject(s)
C-Reactive Protein/biosynthesis , Carcinoma, Squamous Cell/metabolism , Tongue Neoplasms/metabolism , Apoptosis/drug effects , C-Reactive Protein/pharmacology , Carcinoma, Squamous Cell/pathology , Cell Line, Tumor , Cell Movement/drug effects , Cell Proliferation/drug effects , Female , Humans , Lymphatic Metastasis , Male , Middle Aged , Phosphorylation/drug effects , Proto-Oncogene Proteins c-akt/metabolism , TOR Serine-Threonine Kinases/metabolism , Tongue Neoplasms/pathology
12.
J Asthma ; 54(3): 300-307, 2017 Apr.
Article in English | MEDLINE | ID: mdl-27435534

ABSTRACT

CONTEXT: Reslizumab is a humanised anti-interleukin 5 monoclonal antibody that disrupts eosinophil maturation and promotes programmed cell death. OBJECTIVE: We carried out a systematic review and meta-analysis to assess the efficacy and safety of the drug in patients with inadequately controlled, eosinophilic asthma. DATA SOURCES: The search included the following databases: MEDLINE, EMBASE, and the Cochrane Controlled Trials Register. STUDY SELECTION: A literature review was performed to identify all published randomized double-blind, placebo-controlled trials of reslizumab for the treatment of inadequately controlled, eosinophilic asthma. DATA EXTRACTION: Two reviewers independently extracted and verified pre-defined data fields. RESULTS: Four publications including 5 RCTs that compared reslizumab with placebo. For the comparison of reslizumab with placebo, asthma exacerbation (odds ratio (OR) = 0.46, 95% confidence interval (CI) = 0.35 to 0.59, p <0.00001); a forced expiratory volume in 1 s (FEV1) (the standardized mean difference (SMD) = 0.16, 95%CI = 0.10 to 0.23, p <0.00001); Asthma Control Questionnaire (ACQ) score (the SMD = -0.26, 95%CI= -0.36 to -0.16, p <0.00001); blood eosinophil counts (the SMD = -475.62, 95%CI = -528.41 to -422.83, p <0.00001). Safety assessments included the proportion of individuals who withdrawn due to adverse event (AE) (OR = 0.60 95%CI = 0.38 to 1.17, p = 0.16) indicated that reslizumab was well tolerated. LIMITATIONS: The article didn't research the safety, efficacy of reslizumab with longer term. CONCLUSIONS: This meta-analysis indicates that reslizumab to be an effective and safe treatment for inadequately controlled, eosinophilic asthma.


Subject(s)
Anti-Asthmatic Agents/therapeutic use , Antibodies, Monoclonal, Humanized/therapeutic use , Asthma/drug therapy , Asthma/epidemiology , Eosinophilia/epidemiology , Quality of Life , Anti-Asthmatic Agents/administration & dosage , Anti-Asthmatic Agents/adverse effects , Antibodies, Monoclonal, Humanized/administration & dosage , Antibodies, Monoclonal, Humanized/adverse effects , Double-Blind Method , Humans , Randomized Controlled Trials as Topic , Respiratory Function Tests
13.
Childs Nerv Syst ; 33(9): 1583-1587, 2017 Sep.
Article in English | MEDLINE | ID: mdl-28664277

ABSTRACT

OBJECT: Congenital arteriovenous fistula involving the external carotid system is rare. METHODS: This paper reports a case of congenital external carotid artery-external jugular vein arteriovenous fistula admitted to Xuanwu Hospital, and reviews the literature. RESULTS: The patient was a boy, 9 years old, with a history of pulsatile mass and thrill in the right neck since his birth. External carotid artery-external jugular vein fistula was confirmed by the digital subtraction angio-graphy. And coil embolization was done later. Postoperative immediate angiography confirmed the complete occlusion of the fistula, and partial branch of the external carotid artery can be seen. The abnormal clinical manifestation disappeared after the procedure without any complications. CONCLUSION: This case and relevant literatures remind us that congenital external carotid artery-external jugular vein arteriovenous fistula has its unique features, and it can be treated by coil embolization safely and effectively.


Subject(s)
Arteriovenous Fistula/therapy , Carotid Artery, External/abnormalities , Embolization, Therapeutic/methods , Jugular Veins/abnormalities , Child , Humans , Male
14.
J Infect ; 88(5): 106143, 2024 May.
Article in English | MEDLINE | ID: mdl-38548243

ABSTRACT

BACKGROUND: Next-generation sequencing (NGS) might aid in the identification of causal pathogens. However, the optimal approaches applied to cerebrospinal fluid (CSF) for detection are unclear, and studies evaluating the application of different NGS workflows for the diagnosis of intracranial infections are limited. METHODS: In this multicenter, prospective observational cohort study, we described the diagnostic efficacy of pathogen-targeted NGS (ptNGS) and metagenomic NGS (mNGS) compared to that of composite microbiologic assays, for infectious meningitis/encephalitis (M/E). RESULTS: In total, 152 patients diagnosed with clinically suspected M/E at four tertiary hospitals were enrolled; ptNGS and mNGS were used in parallel for pathogen detection in CSF. Among the 89 patients who were diagnosed with definite infectious M/E, 57 and 39 patients had causal microbial detection via ptNGS and mNGS, respectively. The overall accuracy of ptNGS was 65.1%, with a positive percent agreement (PPA) of 64% and a negative percent agreement (NPA) of 66.7%; and the overall accuracy of mNGS was 47.4%, with a PPA of 43.8% and an NPA of 52.4% after discrepancy analysis. There was a significant difference in the detection efficiency between these two methods both for PPA (sensitivity) and overall accuracy for pathogen detection (P < 0.05). CONCLUSIONS: NGS tests have provided new information in addition to conventional microbiologic tests. ptNGS seems to have superior performance over mNGS for common causative pathogen detection in CSF for infectious M/E.


Subject(s)
High-Throughput Nucleotide Sequencing , Metagenomics , Humans , High-Throughput Nucleotide Sequencing/methods , Prospective Studies , Female , Male , Adult , China , Middle Aged , Metagenomics/methods , Encephalitis/diagnosis , Encephalitis/microbiology , Encephalitis/cerebrospinal fluid , Young Adult , Aged , Meningitis/diagnosis , Meningitis/microbiology , Meningitis/cerebrospinal fluid , Sensitivity and Specificity , Adolescent , Meningitis, Bacterial/diagnosis , Meningitis, Bacterial/microbiology , Meningitis, Bacterial/cerebrospinal fluid
15.
Hum Cell ; 36(1): 223-233, 2023 Jan.
Article in English | MEDLINE | ID: mdl-36352311

ABSTRACT

Cl-amidine has been reported to have anti-inflammatory properties in a variety of diseases. However, the role of Cl-amidine in periodontal disease remains unclear. Here, the purpose of this study was to investigate the effect of Cl-amidine on lipopolysaccharide (LPS)-induced inflammation in human gingival fibroblasts (HGFs). The cytotoxic effect of Cl-amidine was measured with the Cell Counting Kit-8 (CCK-8) assay and Annexin V-FITC/PI staining. The protein levels of IL-6 and IL-8 in culture supernatants were measured with enzyme-linked immunosorbent assay (ELISA). The mRNA levels of inflammatory cytokines, TLR4 and MyD88 were assessed by quantitative real-time polymerase chain reaction (qRT-PCR) analysis. The expression patterns of IL-6, TNF-ɑ, and IL-1ß in HGFs were tested with western blot. The levels of NF-κB, MAPK, and Nrf2 pathway-related proteins were detected by western blot. Immunofluorescence (IF) staining was used to examine the nuclear translocation of NF-κB p65. Moreover, a rat gingivitis model was established to further clarify the role of Cl-amidine. Our results showed that Cl-amidine suppressed LPS-induced gingival inflammation both in vitro and in vivo. Mechanistically, Cl-amidine inhibited LPS-induced MyD88 expression, NF-κB activation, and JNK phosphorylation. Additionally, Cl-amidine upregulated Nrf2 and Ho-1 expression both with and without LPS stimulation but did not alter ROS levels or Keap1 expression. Overall, our data suggest that Cl-amidine acts as an inhibitor of LPS-induced gingival inflammation via the JNK/MAPK, NF-κB, and Nrf2 signalling pathways.


Subject(s)
Lipopolysaccharides , NF-kappa B , Humans , Rats , Animals , NF-kappa B/metabolism , Lipopolysaccharides/toxicity , Kelch-Like ECH-Associated Protein 1/metabolism , NF-E2-Related Factor 2/genetics , NF-E2-Related Factor 2/metabolism , Interleukin-6/metabolism , Myeloid Differentiation Factor 88/metabolism , Myeloid Differentiation Factor 88/pharmacology , Inflammation/chemically induced , Inflammation/drug therapy , Fibroblasts/metabolism
16.
Sci Total Environ ; 864: 161116, 2023 Mar 15.
Article in English | MEDLINE | ID: mdl-36566852

ABSTRACT

In this study, the hydration mechanism and environmental impacts of blended cements with the co-combustion ash of rice husk and sewage sludge (CCA) were investigated and compared to those of blended cements with sewage sludge ash (SSA). CCA possesses lower phosphate contents than SSA, leading to lower inhibition effects on early hydration of cement clinker. Moreover, the pozzolanic activity of CCA is higher than that of SSA. Thus, more hydration products from the pozzolanic reaction of CCA are generated in CCA-based blended cements. Compared to the matrix of SSA-based blended cements, that of their CCA-based counterpart is filled with more hydration products, which promotes porosity refinement and strength development of CCA-based blended cements at later ages. CCA-based blended cements exhibit greater environmental benefits than SSA-based blended cements because fossil consumption and toxic substance emissions during the co-combustion of rice husk and sewage sludge is lower than that during the mono-combustion of sewage sludge.


Subject(s)
Oryza , Sewage , Environment , Coal Ash
17.
Materials (Basel) ; 16(2)2023 Jan 05.
Article in English | MEDLINE | ID: mdl-36676268

ABSTRACT

In this paper, preferential enrichment (PE) is described for three pairs of novel amino acid Schiff base Cu(II)/Cu(I) complexes. Single crystal X-ray diffraction indicated that 1-S/R are one-dimensional coordination polymers (CPs) with helical structures, and 2-S/R and 3-S/R are one-dimensional CPs with auxiliary ligands. By tuning the pH, the solvent and second ligands, the 1-S/R, 3-S/R underwent polymorphic transitions, resulting in enantioselective liberation of excess enantiomers into solution, until the deposited crystals were slightly enriched with the opposite enantiomer, thereby successfully exhibiting PE. However, under the effects of Cu(II), the solvent and low pH, 2-S/R did not exhibit PE and resulted in enrichment of racemic compounds, which was attributed to amino acid Schiff base chiral complex mechanisms of PE. The three pairs of Cu complex structures were characterized by UV-vis, MS and X-ray photoelectron spectroscopy (XPS). All chiral properties were studied by circular dichroism (CD) in the solid and liquid.

18.
Hum Cell ; 36(4): 1485-1500, 2023 Jul.
Article in English | MEDLINE | ID: mdl-36964413

ABSTRACT

Drug resistance is a great obstacle to the clinical application of paclitaxel (PTX) in breast cancer treatment. Chemoresistance can be either primary or acquired. Multifarious factors are related to drug resistance. Among these factors, drug-induced autophagy has been shown to contribute to acquired chemoresistance in cancer cells. Additionally, cancer stem cells (CSCs) drive primary chemoresistance. Recent advances regarding TIPE2 demonstrate that TIPE2 enhances osteosarcoma and non-small cell lung cancer cell sensitivity to cisplatin. However, the role of TIPE2 in PTX resistance in breast cancer cells has not been elucidated. Here, the in vitro and in vivo study demonstrated that TIPE2 sensitized breast cancer cells to PTX by suppressing drug-induced autophagy and CSC properties. Mechanistically, we found that TIPE2 activated the AKT/mTOR signalling pathway and inhibited the TAK1/MAPK signalling pathway to suppress drug-induced autophagy. Moreover, TIPE2 inhibited TAK1/NF-κB activation to reduce breast CSC properties. Collectively, our results first elucidated the inhibitory role of TIPE2 in breast cancer chemoresistance. Thus, TIPE2 may be a new target for breast cancer chemotherapy.


Subject(s)
Bone Neoplasms , Breast Neoplasms , Carcinoma, Non-Small-Cell Lung , Lung Neoplasms , Humans , Female , Paclitaxel/pharmacology , Breast Neoplasms/drug therapy , Breast Neoplasms/genetics , Intracellular Signaling Peptides and Proteins/genetics , Cell Line, Tumor , Autophagy/genetics , Neoplastic Stem Cells , Drug Resistance, Neoplasm/genetics
19.
Childs Nerv Syst ; 26(7): 889-96, 2010 Jul.
Article in English | MEDLINE | ID: mdl-20107995

ABSTRACT

OBJECTIVE: The objective of this study was to review our pediatric series of perimedullary arteriovenous fistula (PMAVF), investigating the clinical characteristics, angioarchitecture, treatment strategy, and outcome of PMAVF in pediatric patients. METHODS AND MATERIALS: Nineteen children with PMAVF treated in Xuanwu Hospital between 1986 and 2007 were reviewed. The demographic data, clinical symptoms, angiographical characteristics, classification, treatment modality, and outcome were compiled and analyzed. Clinical status was evaluated according to the modified Aminoff and Logue scale. RESULTS: In our pediatric series of PMAVF, the mean age of diagnosis was 8.1 years (range, 0.8-13.7 years). Male predominance was found in our series (M/F ratio, 3.75:1). Eleven children (57.9%) presented with acute onset of symptoms, and eight cases (42.1%) had progressive evolution of the diseases. In 13 cases (68.4%), the fistulas were classified as type III, with type II in six cases. Four lesions (21.1%) were located in the cervical region, with 15 lesions (78.9%) in thoracolumbar region. Ten cases (II = 2, III = 8) were treated with embolization, and nine (II = 4, III = 5) with combined embolization and complementary surgery. According to the criteria of therapeutic effectiveness, 31.6% of cases (6/19) were cured, and nine patients (47.4%) had improved. CONCLUSIONS: PMAVFs in pediatric population exhibit many characteristics in clinical and angiographical aspects, need for special considerations. Most of PMAVFs in pediatric patients are high-flow fistulas, and endovascular embolization should be the first choice of treatment. After appropriate therapy, most of children can get satisfactory outcomes.


Subject(s)
Arteriovenous Fistula/pathology , Arteriovenous Fistula/therapy , Arteriovenous Malformations/pathology , Arteriovenous Malformations/therapy , Spinal Cord/pathology , Adolescent , Arteriovenous Fistula/epidemiology , Arteriovenous Malformations/epidemiology , Child , Child, Preschool , Disease Progression , Embolization, Therapeutic , Female , Follow-Up Studies , Humans , Infant , Magnetic Resonance Angiography , Magnetic Resonance Imaging , Male , Neurosurgical Procedures , Prognosis , Sex Factors , Spinal Cord/abnormalities , Treatment Outcome
20.
J Int Med Res ; 48(8): 300060520934254, 2020 Aug.
Article in English | MEDLINE | ID: mdl-32776855

ABSTRACT

OBJECTIVE: Long noncoding (lnc)RNAs regulate multiple biological processes including cancer. Oral squamous cell carcinoma (OSCC) is a common malignancy with poor prognosis. We aimed to identify the function of lncRNA HOXA10 antisense RNA (HOXA10-AS) and its clinical significance. METHODS: We used differential expression analysis to identify aberrantly expressed lncRNAs associated with OSCC. We identified key genes related to HOXA10-AS and their biological functions using bioinformatics tools and functional enrichment analyses. We predicted the function of HOXA10-AS using gene set enrichment and variation analyses and analyzed proliferation markers at the mRNA and protein levels. Finally, we silenced HOXA10-AS using antisense oligonucleotide and assessed proliferation ability using a cell counting kit (CCK8) and clone formation assays. RESULTS: In total, 506 aberrantly expressed lncRNAs were identified. HOXA10-AS was identified as a risk factor for OSCC and its expression was positively associated with tumor grade. We identified hub genes involved in regulating proliferation and predicted that HOXA10-AS is associated with an active cell cycle and increased proliferation. Silencing HOXA10-AS decreased proliferation in OSCC cell lines. CONCLUSIONS: HOXA10-AS is involved in cell proliferation and silencing it decreases proliferation. Thus, HOXA10-AS could serve as prognostic biomarker and therapeutic target for OSCC.


Subject(s)
Carcinoma, Squamous Cell , Mouth Neoplasms , RNA, Long Noncoding , Carcinoma, Squamous Cell/genetics , Cell Line, Tumor , Cell Proliferation/genetics , Gene Expression Regulation, Neoplastic , Homeobox A10 Proteins , Humans , Mouth Neoplasms/diagnosis , Mouth Neoplasms/genetics , Prognosis , RNA, Antisense/genetics , RNA, Long Noncoding/genetics
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