Subject(s)
Antibodies, Monoclonal , Cyclosporins/blood , Kidney Transplantation , Biotransformation , Chromatography, High Pressure Liquid , Cross Reactions , Cyclosporins/adverse effects , Cyclosporins/metabolism , Cyclosporins/therapeutic use , Graft Rejection , Humans , Kidney Diseases/chemically induced , Radioimmunoassay/standardsSubject(s)
Cyclosporins/pharmacokinetics , Radioimmunoassay/methods , Animals , Antibodies, Monoclonal , Cell Line , Chromatography, High Pressure Liquid , Cross Reactions , Cyclosporins/therapeutic use , Humans , Interleukin-2/biosynthesis , Lymphocyte Activation/drug effects , Mice , Structure-Activity RelationshipABSTRACT
We evaluated a new IRMA developed commercially for the measurement of corticotropin (ACTH) in human plasma. The assay involves purified polyclonal goat capture antibodies specific for ACTH 26-39 and an 125I-labeled monoclonal signal antibody specific for ACTH 1-17. CVs for intraassay and total precision at ACTH concentrations between 9 and 801 ng/L ranged from 2.5% to 4.7% and from 3.3% to 9.3%, respectively, with an assay detection limit of 1.7 ng/L. The reference interval determined for adults with the new method (16-52 ng/L) differed significantly (P < 0.05) from that for an established ACTH IRMA (9-54 ng/L). Method comparison with clinical samples (n = 179) revealed a correlation coefficient of 0.970 and a best-fit equation of y (new IRMA) = (1.011 +/- 0.019)x + (4.17 +/- 3.31) with Sylx = 40.2. Both methods showed equivalent clinical sensitivity in evaluating Cushing disease, adrenal tumors, ectopic ACTH-producing tumors, hypopituitarism, steroid suppression, surgical adrenalectomy, Nelson syndrome, Addison disease, and corticotropin-releasing hormone stimulation. We conclude that the new IRMA is technically simple to perform and provides a specific and sensitive method for evaluating of adrenocortical function.