Effect of graded levels of soapnut (Sapindus mukorossi) shell powder on reproductive performance in broiler breeders.

OBJECTIVE: This study investigated the effects of soapnut (Sapindus mukorossi) shell powder (SSP) on serum hormone level, egg quality, semen characteristics and reproductive performance of broiler breeders fed with a maize-soybean meal based diet. METHODS: Ninety six female and twenty four male CARIBRO-VISHAL broiler breeders, 38-week old, were individually caged and randomly allocated to four treatment groups (24 female breeders/treatment and 6 male breeders/treatment): an un-supplemented control (T1) and three groups with 0.0176% SSP (group T2), 0.026% SSP (group T3) and 0.0528% SSP (group T4), to have supplementary saponin at 0, 50, 75, and 150 ppm, respectively, for 42 days. RESULTS: The results indicated that serum (p<0.001) and seminal plasma (p<0.05) testosterone level, semen volume (p<0.001), mass motility (p<0.001), and live spermatozoa count (p<0.001) was increased in groups T3 and T4 compared to T2 and control groups. Compared with control group, total sperm count was increased (p<0.001) and dead spermatozoa count was decreased (p<0.001) in SSP supplemented groups. Supplementation of SSP did not affected the quality of egg lay. Compared with control group, fertility (p<0.01) and hatchability (total eggs set and fertile eggs set) (p<0.001) were significantly improved in SSP supplemented groups with the highest improvement in T3 treatment group. Embryonic death was decreased (p< 0.001) in SSP supplemented groups compared to control; lowest embryonic death was recorded in T3 treatment group. CONCLUSION: Thus, it was concluded that dietary supplementation of 0.026% SSP (saponin equivalent 75 ppm) improved the reproductive performance of broiler breeders.

Purification and dose-dependent toxicity study of abrin in swiss albino male mice.

Abrin, a phytotoxin obtained from the seeds of the Abrus precatorius plant, is highly toxic with an estimated human fatal dose of 0.1­1 µg/kg. In this study, abrin was purified and characterized through SDS PAGE and mass spectrometry analysis; further study on toxicity was carried out to investigate the alteration in biochemical, and hematological variables through histopathological observations in mice. The intraperitoneal LD50 value of purified abrin for mice was found to be 0.91µg/kg of body weight. Mice were exposed to 0.4 and 1.0 LD50 abrin doses intraperitoneally and observed on days 1, 3, and 7. Plasma GOT and GPT levels increased significantly at both doses. At 1.0 LD50 dose, alkaline phosphatase, bilirubin, urea, uric acid, and creatinine levels increased, whereas albumin, total protein, glucose and cholesterol levels decreased significantly. Abrin intoxication also altered the hemoglobin, WBC, and RBC counts significantly at 1.0 LD50 dose. Liver GSH levels decreased while lipid peroxidation increased significantly in a dose­dependent manner. Biochemical changes were supported by the histological investigation, which also showed the degenerative changes in organs. In conclusion, abrin intoxication caused toxic effects and severe damages on studied organs mediated through alteration in biochemical and hematological variables, lipid peroxidation, and degeneration.

Effect of Zinc Nanoparticles on Milk Yield, Milk Composition and Somatic Cell Count in Early-Lactating Barbari Does.

Nano minerals have better bioavailability, efficient utilization, smaller dose rate, and stable interactions within the biological system. Efficacy of dietary supplementation of nano zinc particles on milk yield, milk composition, and somatic cell count was studied in periparturient Barbari does. Twenty-four Barbari goats (mean body weight 35 ± 1.5 kg) of about 4 months of pregnancy were divided into four groups (Gr I, Gr II, Gr III, and Gr IV) of six animals each. All the does were fed a common basal diet (containing 38.96 mg Zn/kg DM) of concentrate pellet and Bengal gram (Cicer arietinum) straw. No zinc was supplemented in does of Gr I (control group), while Gr II, Gr III, and Gr IV does were supplemented with 20 ppm inorganic zinc oxide, 10 ppm of nano zinc, and 20 ppm of nano zinc respectively. The experimental feeding was done for 75 days (30 days before kidding to 45 days after kidding). Results showed that yield and composition of milk in different groups of lactating does were not significantly (P < 0.05) affected by zinc supplementation. The percentages of solid-nonfat, fat, protein, lactose, and total solid were comparable (P > 0.05) among different groups of lactating does. The milk mineral levels of Zn, Cu, Na, Ca, and K were statistically similar (P > 0.05) in all the groups of goats. There was significantly (P < 0.05) lower somatic cell count (SCC) in zinc-supplemented groups (Gr II, Gr III, and Gr IV) as compared with the control (Gr I) group of does. SCC was lowest in Gr IV followed by Gr III, Gr II, and Gr I. The present study concluded that supplementation of inorganic zinc @ 20 ppm and nano zinc @ 10 and 20 ppm had no significant effect on milk yield/composition but reduced milk somatic cell count. The efficacy of nano zinc was found to be better as compared with normal inorganic form.

Gallstone Bezoar Following Cholecystogastric Fistula: a Rare Sequelae of Cholelithiasis.

Cholecystogastric fistula is a rare biliary-enteric fistula with a variable clinical presentation. Despite modern diagnostic tools, a high index of suspicion is needed to diagnose it preoperatively. Stone migration into stomach forming gallstone bezoar is very rare. Stones more than 2.5 cm are likely to cause obstruction. We report a case of gallstone bezoar of size 9 × 5 cm lying in the stomach with a small fistulous opening in the prepyloric region of the stomach. Patient was thoroughly investigated and successfully treated with retrieval of bezoar and cholecystectomy.

Evaluation of wound-healing formulation against sulphur mustard-induced skin injury in mice.

Sulphur mustard (SM) is a bifunctional alkylating agent that causes cutaneous blisters in human and animals. Remedies to SM-induced dermatotoxicity are still in experimental stage. Due to inevitable requirement of a wound-healing formulation against SM-induced skin lesions, efficacy of formulations including povidone iodine, Aloe vera gel, betaine or framycetin sulphate was evaluated in present study. SM was applied percutaneously (5 mg/kg) once on back region of Swiss albino mice; and after 24 hours, DRDE/WH-02 (Defence Research and Development Establishment/ Wound Healant- 02, containing polyvinylpyrrolidone [PVP], A. vera gel and betaine), Ovadine, Soframycin or A. vera gel were applied topically, daily for 3 or 7 days in different groups. Skin sections were subjected to histopathology, histomorphologic grading, tissue leukocytosis, terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL) assay and immunohistochemistry of inflammatory-reparative biomarkers. DRDE/WH-02 treated mice received highest score on the basis of histomorphologic scale and lowest number of TUNEL-positive cells compared to other groups. DRDE/WH-02 showed better wound healing as evidenced by widespread re-epithelialization, homogenous fibroplasias well supported by the expression of transforming growth factor-α, endothelial nitric oxide synthase (eNOS) and fibroblast growth factor. Upregulation of interleukin 6 in DRDE/WH-02-treated mice skin resulted in increased tissue leukocytosis and an early removal of tissue debris that initiated reparative process at faster rate compared to other groups. In conclusion, DRDE/WH-02 provided better healing effect and can be recommended as an effective wound healant against SM-induced skin injury.