Structural and functional study for tRNA cleavage by Glycine o-phenanthroline CuII complex, [CuCl(phen)(gly)]∙4H2O.

The o-phenanthroline gly Cu(II) complex, [CuCl(phen)(gly)]∙4H2O 1, has been prepared and structurally characterized. The transfer RNA binding and degradation properties of complex 1 have been investigated by UV-vis spectroscopy, cyclic voltammetry (CV), capillary electrophoresis (CE) and atomic force microscopy (AFM) methods. The results showed that 1 can efficiently cleave tRNA in the physiological conditions (pH 7.0, and 37 °C), and has a digestion coefficient nearly up to 100% within 75 h. AFM image for 1/RNA exhibited arrayed tandem repetitions of tRNA segments. This study is targeting the destruction of the high-order structures of genetic biomacromolecules which paves an important way to novel materials for the decontamination of microorganisms (e.g., bacteria and viruses).

Altered spontaneous brain activity in Cushing's disease: a resting-state functional MRI study.

CONTEXT AND OBJECTIVE: Cushing's disease (CD) provides a unique and naturalist model for studying the influence of hypercortisolism on the human brain and the reversibility of these effects after resolution of the condition. This cross-sectional study used resting-state fMRI (rs-fMRI) to investigate the altered spontaneous brain activity in CD patients and the trends for potential reversibility after the resolution of the hypercortisolism. We also aim to determine the relationship of these changes with clinical characteristics and cortisol levels. SUBJECTS AND METHODS: Active CD patients (n = 18), remitted CD patients (n = 14) and healthy control subjects (n = 22) were included in this study. Amplitude of low-frequency fluctuation (ALFF) and regional homogeneity (ReHo) values were calculated to represent spontaneous brain activity. RESULTS: Our study resulted in three major findings: (i) active CD patients showed significantly altered spontaneous brain activity in the posterior cingulate cortex (PCC)/precuneus (PCu), occipital lobe (OC)/cerebellum, thalamus, right postcentral gyrus (PoCG) and left prefrontal cortex (PFC); (ii) trends for partial restoration of altered spontaneous brain activity after the resolution hypercortisolism were found in several brain regions; and (iii) active CD patients showed a significant correlation between cortisol levels and ALFF/ReHo values in the PCC/PCu, a small cluster in the OC and the right IPL. CONCLUSIONS: This study provides a new approach to investigating brain function abnormalities in patients with CD and enhances our understanding of the effect of hypercortisolism on the human brain. Furthermore, our explorative potential reversibility study of patients with CD may facilitate the development of future longitudinal studies.

Volumetric magnetic resonance imaging analysis in patients with short-term remission of Cushing's disease.

OBJECTIVE: The data on patients with short-term remission of Cushing's disease (CD) might provide information that is not available from previous long-term remission studies. We aimed to investigate structural changes in the brain in these patients and to examine whether these changes were associated with clinical characteristics. DESIGN: A cross-sectional study was performed. METHODS: Thirty-four patients with CD (14 with CD in short-term remission and 20 with active CD) and 34 controls matched for age, sex and education underwent clinical evaluation and magnetic resonance imaging brain scans. Biometric measurements, disease duration and remission duration data were collected. Grey matter volumes in the whole brain were examined using voxel-based morphometry (VBM). RESULTS: No differences were observed in the grey matter volumes of the medial frontal gyrus (MFG) and cerebellum between the patients with remitted CD and healthy controls, whereas patients with active CD had smaller grey matter volumes in these two regions compared with controls and patients with remitted CD. Furthermore, significant correlations were found between remission time and grey matter values in these regions in short-term remission patients with CD. Additionally, greater grey matter volumes in the bilateral caudate of short-term remission patients with CD were observed. CONCLUSIONS: Trends for structural restoration were found in CD patients with short-term remission. This finding was associated with the number of days elapsed since curative surgery and the current age of the patients. This study enhances our understanding of potential reversibility after the resolution of hypercortisolism in CD patients.

5,6-Diphenyl-3-(3-pyrid-yl)-1,2,4-triazine.

In the mol-ecule of the title compound, C(20)H(14)N(4), the triazine ring is attached to two phenyl rings and one pyridine ring. In the crystal, mol-ecules are linked by inter-molecular C-H⋯N hydrogen bonds. The crystal packing is also stabilized by C-H⋯π inter-actions.

4-[Bis(3,4-dimethoxy-phen-yl)meth-yl]pyridine ethanol monosolvate.

In the title compound, C(22)H(23)NO(4)·C(2)H(6)O, the pyridyl ring is aligned at 89.39 (2) and 87.41 (2)° with respect to the benzene rings, and the three rings connected to the methine C atom are arranged in a propeller-like conformation. The heterocycle is linked to the solvent mol-ecule by an O-H⋯N hydrogen bond.

1-[4-(Dimethyl-amino)benzyl-idene]-4-methyl-thio-semicarbazide.

In the title compound, C(11)H(16)N(4)S, an intra-molecular N-H⋯N hydrogen bond generates an S(5) ring. In the crystal, inversion dimers linked by pairs of N-H⋯S bonds occur, generating an R(2) (2)(8) loop.

N'-(4-Methyl-benzyl-idene)thio-phene-2-carbohydrazide.

In the title compound, C(13)H(12)N(2)OS, the dihedral angle between the aromatic rings is 14.84 (17)°. In the crystal, inversion dimers linked by pairs of N-H⋯O hydrogen bonds generate R(2) (2)(8) loops.

4-Ethyl-1-(4-methyl-benzyl-idene)thio-semicarbazide.

In the title compound, C(11)H(15)N(3)S, an intra-molecular N-H⋯N hydrogen bond generates an S(5) ring. In the crystal, inversion dimers linked by pairs of N-H⋯S bonds occur, generating an R(2) (2)(8) loop.

(E)-N'-(4-Methoxy-benzyl-idene)thio-phene-2-carbohydrazide.

In the title compound, C(13)H(12)N(2)O(2)S, the dihedral angle between the aromatic rings is 15.20 (11)°. In the crystal, inversion dimers linked by pairs of N-H⋯O hydrogen bonds generate R(2) (2)(8) loops.

N'-(4-Cyano-benzyl-idene)furan-2-carbohydrazide monohydrate.

In the title compound, C(13)H(9)N(3)O(2)·H(2)O, the dihedral angle between the aromatic rings is 10.7 (4)° and an intra-molecular N-H⋯O hydrogen bond occurs. In the crystal, the components are linked by N-H⋯O, O-H⋯N and O-H⋯O hydrogen bonds.

4-Ethyl-1-(4-meth-oxy-benzyl-idene)thio-semicarbazide.

In the title compound, C(11)H(15)N(3)OS, the dihedral angle between the aromatic ring and the thio-urea unit is 4.28 (7)° and an intra-molecular N-H⋯N hydrogen bond generates an S(5) ring. In the crystal, mol-ecules are linked into (001) sheets by N-H⋯S hydrogen bonds.

4-Ethyl-1-[4-(methyl-sulfan-yl)benzyl-idene]thio-semicarbazide.

There are four independent mol-ecules in the asymmetric unit of the title compound, C(11)H(15)N(3)S(2), with different conformations: the dihedral angles between the benzene rings and thio-urea units are 16.85 (9), 0.56 (10), 8.05 (12) and 4.56 (8)°. Each mol-ecule contains an intra-molecular N-H⋯N hydrogen bond, generating an S(5) ring. The crystal structure is stabilized by inter-molecular N-H⋯S hydrogen bonds.

N'-Benzyl-idene-furan-2-carbohydrazide.

In the title compound, C(12)H(10)N(2)O(2), the dihedral angle between the benzene ring and the furan ring is 24.6 (2)°. In the crystal, mol-ecules are linked by N-H⋯O hydrogen bonds, generating C(4) chains propagating in [010].

N'-(3,4-Dimethyl-benzyl-idene)furan-2-carbohydrazide.

The title compound, C(14)H(14)N(2)O(2), was prepared by the reaction of 3,4-dimethyl-benzaldehyde and furan-2-carbohydrazide. The dihedral angle between the aromatic rings is 35.48 (14)°. In the crystal, mol-ecules are linked by N-H⋯O hydrogen bonds, generating C(4) chains propagating in [010].

(E)-N'-[4-(Methyl-sulfan-yl)benzyl-idene]furan-2-carbohydrazide monohydrate.

In the title compound, C(13)H(12)N(2)O(2)S·H(2)O, the dihedral angle between the aromatic rings is 35.34 (19)° and an intra-molecular N-H⋯O hydrogen bond generates an S(5) ring. In the crystal, mol-ecules are linked by N-H⋯O and O-H⋯O hydrogen bonds, generating (001) sheets.

Methyl 3-(4-methyl-benzyl-idene)carbazate.

The title compound, C(10)H(12)N(2)O(2), was prepared by the reaction of methyl carbazate and 4-methyl-benzaldehyde. The dihedral angle between the benzene ring and the carbazate fragment is 20.86 (10)°. In the crystal structure, mol-ecules are linked by inter-molecular N-H⋯O hydrogen bonds.

N'-(4-Cyano-benzyl-idene)thio-phene-2-carbohydrazide.

The title compound, C(13)H(9)N(3)OS, was prepared by the reaction of thio-phene-2-carbohydrazide and 4-formyl-benzonitrile. The dihedral angle between the benzene and thio-phene rings is 11.9 (1)°. In the crystal structure, mol-ecules are linked into centrosymmetric dimers by pairs of N-H⋯O hydrogen bonds.

Bis(2,2'-bipyrid-yl)(dichloro-acetato)copper(II) dichloro-acetate dihydrate.

In the title compound, [Cu(C(2)HCl(2)O(2))(C(10)H(8)N(2))(2)](C(2)HCl(2)O(2))·2H(2)O, the Cu(II) ion is bonded to two N,N'-bidentate 2,2'-bipyridyl ligands and one O-monodentate 2,2-dichloro-acetate anion in a distorted CuON(4) trigonal-bipyramidal geometry, with the O atom occupying an equatorial site. In the crystal, the components are linked by O-H⋯O and O-H⋯Cl hydrogen bonds.

Ethyl 3-(4-methyl-benzyl-idene)carbazate.

There are two mol-ecules in the asymmetric unit of the title compound, C(11)H(14)N(2)O(2), which have similar conformations. In the crystal, the mol-ecules are linked by N-H⋯O hydrogen bonds, generating C(4) chains propagating in [001].

N'-(4-Hy-droxy-benzyl-idene)thio-phene-2-carbohydrazide.

In the title compound, C(12)H(10)N(2)O(2)S, the dihedral angle between the benzene and thio-phene rings is 23.34 (16)°. In the crystal structure, mol-ecules are linked by N-H⋯O and O-H⋯O hydrogen bonds, forming (100) sheets.