ABSTRACT
The prion diseases are neurodegenerative disorders characterized by the conversion of the PrPc (normal cellular prion) to the PrPsc (misfolded isoform). The accumulation of PrPsc within the central nervous system (CNS) leads to neurocytotoxicity by increasing oxidative stress. In addition, many neurodegenerative disorders including prion, Parkinson's and Alzheimer's diseases may be regulated by cholesterol homeostasis. The effects of cholesterol balance on prion protein-mediated neurotoxicity and ROS (reactive oxygen species) generation were the focus of this study. Cholesterol treatment inhibited PrP (106-126)-induced neuronal cell death and ROS generation in SH-SY5Y neuroblastoma cells. In addition, the PrP (106-126)-mediated increase of p53, p-p38, p-ERK and the decrease of Bcl-2 were blocked by cholesterol treatment. These results indicated that cellular cholesterol enrichment is a key regulator of PrP-106-126-mediated oxidative stress and neurotoxicity. Taken together, the results of this study suggest that modulation of cellular cholesterol appears to prevent the neuronal cell death caused by prion peptides.
Subject(s)
Apoptosis , Cholesterol/metabolism , Neurons/physiology , Peptide Fragments/metabolism , Prions/metabolism , Cell Line , Cell Line, Tumor , Cholesterol/pharmacology , Humans , Neurons/drug effects , Peptide Fragments/pharmacology , Prions/pharmacology , Reactive Oxygen Species/metabolismABSTRACT
OBJECTIVES: The aim of this study was to prevent metastatic myocardial calcification and hypertension following chronic renal failure (CRF). METHODS: A total of 50 male Sprague-Dawley rats were allocated to one of five groups: the control group (sham), the NxNT group (nephrectomized rats receiving no treatment), the NxFuro group (nephrectomized rats treated with furosemide), the NxCap group (nephrectomized rats treated with captopril) and the NxFuroCap group (nephrectomized rats treated with furosemide and captopril). Surgery (5/6 nephrectomy) was performed to induce CRF. Oral treatment with furosemide (20 mg/kg) and/or captopril (0.05 mg/kg) was given twice daily for 5 weeks. Parameters were studied after 5 weeks. RESULTS: In the NxNT group, arterial blood pressure was significantly increased compared with the controls. Monotherapy with furosemide or captopril and both in combination maintained blood pressure to near or below control. Myocardial and remnant-kidney calcification was detected in NxNT rats, but calcification was absent in the NxFuroCap rats. Cardiac hypertrophy and fibrosis was observed in the NxNT group but not in treatment groups. Both plasma inorganic phosphate and Ca(2+) significantly increased in the NxNT group, but the difference was not significant in the treatment groups. CONCLUSION: Furosemide, either alone or in combination with captopril, is capable to prevent myocardial calcification, cardiac hypertrophy and hypertension, maintaining blood Ca(2+) and phosphate levels by slowing CRF.
Subject(s)
Antihypertensive Agents/therapeutic use , Calcinosis/prevention & control , Cardiomyopathies/prevention & control , Diuretics/therapeutic use , Hypertension/prevention & control , Kidney Failure, Chronic/drug therapy , Analysis of Variance , Animals , Calcinosis/etiology , Captopril/therapeutic use , Cardiomyopathies/etiology , Disease Models, Animal , Disease Progression , Drug Therapy, Combination , Euthanasia, Animal , Furosemide/therapeutic use , Hemodynamics/drug effects , Hypertension/etiology , Kidney Failure, Chronic/complications , Kidney Failure, Chronic/pathology , Male , Myocardium/pathology , Nephrectomy , Rats , Rats, Sprague-DawleyABSTRACT
Intravascular oxidation is a respiratory assist method used to treat acute respiratory distress syndrome (ARDS). However intravascular oxidation through higher gas exchange is needed for successful clinical applications. In this study, an attempt was made to improve the gas exchange of an intravascular lung assist device by decreasing the level of damage to the blood through the microencapsulation of hemoglobin. The results showed that a hemosome 0.8 microm in diameter could be produced by microencapsulating the hemoglobin extracted from fresh bovine blood with the phospholipids extracted from egg yolk. The oxygen saturation curve of hemosome was S-shaped, which is similar to that found in normal blood, and the P50 was 24 mmHg. The oxygen saturation in the mixed solution of hemosome and blood at a 1:4 (v/v%) ratio was similar to that of normal blood. The gas exchange of the blood-hemosome mixed solution was more effective than whole blood. Therefore, the hemosome solution is expected to improve oxygen transfer.
Subject(s)
Blood Substitutes/chemical synthesis , Hemoglobins/chemistry , Membranes, Artificial , Oxygenators, Membrane , Pulmonary Gas Exchange , Animals , Cattle , Drug Compounding , Equipment Design , Oxidation-ReductionABSTRACT
3-D surgical planning for restorative osteotomy is costly and time-consuming because surgeons need to be helped from commercial companies to get 3-D printed bones. However, practitioners can save time and keep the cost to a minimum by utilizing free software and establishing their 3-D printers locally. Surgical planning for the corrective osteotomy of antebrachial growth deformities (AGD) is challenging for several reasons (the nature of the biapical or multiapical conformational abnormalities and lack of a reference value for the specific breed). Pre-operative planning challenges include: a definite description of the position of the center of rotation of angulation (CORA) and proper positioning of the osteotomies applicable to the CORA. In the present study, we demonstrated an accurate and reproducible bone-cutting technique using patient-specific instrumentations (PSI) 3-D technology. The results of the location precision showed that, by using PSIs, the surgeons were able to accurately replicate preoperative resection planning. PSI results also indicate that PSI technology provides a smaller standard deviation than the freehand method. PSI technology performed in the distal radial angular deformity may provide good cutting accuracy. In conclusion, the PSI technology may improve bone-cutting accuracy during corrective osteotomy by providing clinically acceptable margins.
ABSTRACT
Lipopolysaccharide (LPS) can cause damage to the epithelia of the respiratory tract. However, taurine can protect the lung tissue from such oxidant-induced inflammation. This study examined the effects of a LPS treatment on the intracellular calcium levels ([Ca(2+)]i) as well as the specific mechanisms of LPS-induced cell death in pneumocytes. In addition, the effects of taurine on the LPS-induced increase in the accumulation of reactive oxygen species (ROS) in pneumocytes were investigated. The [Ca(2+)]i in cultured pneumocytes was determined using microfluorescence techniques. The level of activation of the mitogen-activated protein kinases (MAPKs) and Bax protein were measured by Western blotting. LPS at 10 and 100 ng/ml induced cell death and decreased the viability of MRC-5 cells. Moreover, the intracellular Ca(2+) and ROS levels were increased by LPS. The LPS treatment led to the phosphorylation of ERK1/2, JNK and the activation of Bax. A pretreatment with 20 mM taurine reduced the LPS-induced production of ROS and MARK activity. These results show that a LPS treatment induces cell death in MRC-5 cells by increasing the intracellular ROS and Ca(2+) levels. The increase in the intracellular level of ROS promotes MAPKs activation and Bax translocation. Overall, LPS induces lung cell death by activating MAPKs. Furthermore, taurine decreased the LPS-induced generation of ROS and activation of MAPK and Bax.
Subject(s)
Alveolar Epithelial Cells/drug effects , Antioxidants/pharmacology , Lipopolysaccharides/antagonists & inhibitors , Lung/cytology , Mitogen-Activated Protein Kinases/metabolism , Reactive Oxygen Species/metabolism , Taurine/pharmacology , bcl-2-Associated X Protein/metabolism , Calcium Signaling/drug effects , Cell Survival/drug effects , Cells, Cultured , Coloring Agents , Enzyme Activation/drug effects , Fibroblasts/drug effects , Humans , Lipopolysaccharides/pharmacology , Lung/drug effects , Tetrazolium Salts , ThiazolesABSTRACT
AIMS: Previous studies reported that FK506 influences bone mineralizing and hypomagnesemia, and also has immune modifying properties. This study examined whether or not the function of Mg2+ in bone metabolism plays a role in the loss of bone volume caused by immunosuppressants. MAIN METHODS: The effects of the FK506 treatment on the intracellular magnesium and lactate dehydrogenase (LDH) activity were examined in cultured human osteoblasts (HOB) cells. The magnesium concentration was determined using microfluorescence techniques and atomic absorption spectrophotometry. Western blotting was used to measure the level of extracellular signal-regulated kinases 1/2 (ERK 1/2) activation. KEY FINDINGS: FK506 (0.1 microM) did not affect cell death in HOB cells after a 24 hour treatment but decreased the level of ERK 1/2 activation. In HOB cells, the mean [Mg2+]i after exposure to a 1 mM extracellular Mg2+ ([Mg2+]o) buffer was 0.53+/-0.01 mM (n=25). Exposure to 100 nM FK506 produced a significant decrease in [Mg2+]i (0.41+/-0.01 mM). The ERK inhibitor (PD98059) and FK506 produced similar effects but they were not cumulative. SIGNIFICANCE: This study examined the role of ERK1/2 activation on the regulation of magnesium in HOB. These results suggest that the inhibition of ERK phosphorylation is an essential intermediate in the effects of FK506 on magnesium. Overall, FK506 causes bone disorders partly by decreasing [Mg2+]i accompanied by the inhibition of ERK 1/2.
Subject(s)
Extracellular Signal-Regulated MAP Kinases/antagonists & inhibitors , Immunosuppressive Agents/pharmacology , MAP Kinase Signaling System/drug effects , Magnesium/metabolism , Osteoblasts/drug effects , Tacrolimus/pharmacology , Cells, Cultured , Humans , Osteoblasts/metabolismABSTRACT
OBJECTIVES: Panax ginseng (PG) widely used for its various pharmacological activities, including effects on diabetes and its complications. This study aims to investigate the effect of PG on mortality-related hypomagnesemia, hyperlactatemia, metabolic acidosis, and other diabetes-induced abnormalities. MATERIALS AND METHODS: Type 1 diabetes was induced by IV injection of alloxan monohydrate 110 mg/kg into New Zealand white rabbits weighing 2-2.5 kg. PG was supplied in drinking water for 20 weeks. The effects of the PG treatment on diabetes were evaluated through hematological and biochemical analysis including ELISA assays for insulin and glycated haemoglobin A1c (HBA1c) before and after PG extract was supplied. RESULTS: The serum glucose, insulin, and HBA1c levels were significantly improved after the PG treatment compared to those found before PG treatment. In addition, Mg2+, lactate, and base deficit, and acidosis was significantly enhanced in treated rabbits. Moreover, PG showed hepato- and renoprotective effect. Likewise, electrolytes, lipid and protein profile were improved. CONCLUSION: The biochemical and hematological analysis data demonstrate that the PG is effective to alleviate the diabetes serious signs.
ABSTRACT
Bee venom (BV) has been used in Oriental medicine to treat inflammatory diseases, such as tendonitis, bursitis, and rheumatoid arthritis, despite the sensitivity of the victims and toxicity of the venom. This study examined the mechanisms for the effects of BV on the cardiovascular system in rats. The arterial pressure and heart rate (HR) were measured in anesthetized rats. In addition, the left ventricular development pressure (LVDP) and total magnesium efflux ([Mg]e) in isolated perfused hearts, the vascular tonic responses in the isolated aorta, and the blood ionic and biochemical changes were determined simultaneously. In the anesthetized rats, the mean arterial pressure, systolic pressure, and pulse pressure were reduced by BV in a dose-dependent manner, even though the HR was increased. BV had no effects on the relaxation of phenylephrine- or KCl-induced contraction of the aortic rings. In the isolated hearts, BV generated a reversible decrease in the LVDP and velocity with changes in pressure, which were accompanied by increases in the HR and [Mg]e. BV increased the plasma ionized and total magnesium concentrations, and decreased the total magnesium level in the red blood cells. The ratio of ionized calcium/ionized magnesium was also decreased by the BV treatment. BV caused a detectable increase in blood creatine kinase, glutamic oxaloacetic transaminase, and lactic dehydrogenase, as well as a decrease in the blood total protein albumin and globulin levels. These results suggest that BV induces cardiovascular depression by decreasing the cardiac pressure and increasing the ionized magnesium concentration in the blood.
Subject(s)
Bee Venoms/pharmacology , Calcium/metabolism , Cardiovascular System/drug effects , Magnesium/metabolism , Ventricular Function, Left/drug effects , Animals , Blood Pressure/drug effects , Blood Pressure/physiology , Cardiovascular System/metabolism , Cardiovascular System/physiopathology , Heart Rate/drug effects , Heart Rate/physiology , Male , Phenylephrine/pharmacology , Potassium Chloride/pharmacology , Rats , Rats, Sprague-Dawley , Vasoconstriction/drug effects , Vasoconstriction/physiology , Vasoconstrictor Agents/pharmacology , Vasodilation/drug effects , Vasodilation/physiology , Ventricular Function, Left/physiologyABSTRACT
Taurine has been reported to influence bone metabolism, and its specific transport system, the taurine transporter, is expressed in osteoblasts. The mean [Mg2+]i was 0.51+/-0.01 mM in normal culture media. Taurine caused an increase in [Mg(2+)]i by 0.72+/-0.04 mM in human osteoblast (HOB) cells. This increment in [Mg2+]i was inhibited significantly by PD98059, nifedipine, lidocaine, and imipramine. Taurine was also shown to stimulate the activation of ERK 1/2. This taurine-stimulated ERK 1/2 activation was inhibited by PD98059. In the present study, taurine was shown to increase cell proliferation and generate an increase in [Mg2+]i accompanied by ERK 1/2 activation in HOB cells.
Subject(s)
Magnesium/metabolism , Mitogen-Activated Protein Kinase 1/metabolism , Mitogen-Activated Protein Kinase 3/metabolism , Osteoblasts/cytology , Osteoblasts/enzymology , Taurine/pharmacology , Cell Proliferation/drug effects , Cells, Cultured , Enzyme Activation/drug effects , Humans , L-Lactate Dehydrogenase/metabolism , Osteoblasts/drug effects , p38 Mitogen-Activated Protein Kinases/metabolismABSTRACT
A change in pH can alter the intracellular concentration of electrolytes such as intracellular Ca2+and Na2+ ([Na+]i) that are important for the cardiac function. For the determination of the role of pH in the cardiac magnesium homeostasis, the intracellular Mg2+ concentration ([Mg2+]i), membrane potential and contraction in the papillary muscle of guinea pigs using ion-selective electrodes changing extracellular pH ([pH]o) or intracellular pH ([pH]i) were measured in this study. A high CO2-induced low [pH]o causes a significant increase in the [Mg2+]i and [Na+]i, which was accompanied by a decrease in the membrane potential and twitch force. The high [pH]o had the opposite effect. These effects were reversible in both the beating and quiescent muscles. The low [pH]o-induced increase in [Mg2+]i occurred in the absence of [Mg2+]o. The [Mg2+]i was increased by the low [pH]i induced by propionate. The [Mg2+]i was increased by the low [pH]i induced by NH4Cl-prepulse and decreased by the recovery of [pH]i induced by the removal of NH4Cl. These results suggest that the pH can modulate [Mg2+]i with a reverse relationship in heart, probably by affecting the intracellular Mg2+ homeostasis, but not by Mg2+ transport across the sarcolemma.
Subject(s)
Magnesium/metabolism , Papillary Muscles/metabolism , Sodium/metabolism , Animals , Cations, Divalent , Female , Guinea Pigs , Heart Ventricles/metabolism , Hydrogen-Ion Concentration , In Vitro Techniques , Ion Transport/physiology , Ion-Selective Electrodes/veterinary , Male , Membrane Potentials/physiology , Propionates/pharmacologyABSTRACT
Human skin diseases are various and induce chronic inflammatory disorders, including psoriasis, atopic dermatitis and certain forms of ichthyosis. Psoriasis is a chronic inflammatory skin disease characterized by circumscribed, red, thickened plaques. Regulation of the balance between growth, differentiation and death is critical to keratinocytes; when altered, epidermal keratinocytes undergo hyperproliferation, abnormal differentiation and inflammatory infiltration. In the present study, we focused on the effects of resveratrol, found in red wine and peanuts, on the cell death of keratinocytes. We additionally studied the mechanism of resveratrol on Sirt1, a class III histone deacetylase, and Akt phosphorylation. Resveratrol caused apoptosis and increased Sirt1 expression in human HaCaT keratinocytes, following a decrease in the p62 protein level. Inhibition of Sirt1 by Sirt1 inhibitor restored cell viability and protein levels. Furthermore, we showed that resveratrol-induced Sirt1 blocked Akt phosphorylation. The present results indicated that resveratrol inhibited the Akt pathways by inducing Sirt1, thus leading to cell death. These data suggest that resveratrol-mediated activation of Sirt1 histone deacetylase may be a potential therapeutic target for skin diseases including psoriasis.
Subject(s)
Keratinocytes/drug effects , Sirtuin 1/metabolism , Stilbenes/toxicity , Apoptosis , Cell Line , Gene Expression Regulation/drug effects , Humans , Keratinocytes/cytology , Keratinocytes/metabolism , Phosphorylation/drug effects , Proto-Oncogene Proteins c-akt/metabolism , RNA-Binding Proteins/metabolism , ResveratrolABSTRACT
OBJECTIVE: To investigate the protective effects of Nigella sativa seed extract (NSSE) against acetaminophen (APAP)-induced hepatotoxicity in TIB-73 cells and rats. METHODS: Toxicity in TIB-73 cells was induced with 10 µmol/L APAP and the protective effects of NSSE were evaluated at 25, 50, 75, 100 µg/mL. For in vivo examination, a total of 30 rats were equally divided into five experimental groups; normal control (vehicle), APAP (800 mg/kg body weight single IP injection) as a hepatotoxic control, and three APAP and NS pretreated (2 weeks) groups (APAP + NSSE 100 mg; APAP + NSSE 300 mg and APAP + NSSE 900 mg/kg). RESULTS: TIB-73 cell viability was drastically decreased by (49.0 ± 1.9)% after the 10 µmol/LAPAP treatment, which also increased reactive oxygen species production. Co-treatment with NSSE at 25, 50, 75, and 100 µg/mL significantly improved cell viability and suppressed reactive oxygen species generation. In vivo, the APAP induced alterations in blood lactate levels, pH, anionic gap, and ion levels (HCO3(-), Mg(2+) and K(+)), which tended to normalize with the NSSE pretreatment. The NSSE also significantly decreased elevated serum levels of alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase, and alkaline phosphatase induced by APAP, which correlated with decreased levels of hepatic lipid peroxidation (malondialdehyde), increased superoxide dismutase levels, and reduced glutathione concentrations. Improved hepatic histology was also found in the treatment groups other than APAP group. CONCLUSIONS: The in vitro and in vivo findings of this study demonstrated that the NSSE has protective effects against APAP-induced hepatotoxicity and metabolic disturbances by improving antioxidant activities and suppressing both lipid peroxidation and ROS generation.
ABSTRACT
The objective of this study was to investigate the therapeutic efficacies of crude yam (Dioscorea batatas) powder (PY), water extract of yam (EY), and allantoin (the active constituent of yam) in streptozotocin (STZ)-induced diabetic rats with respect to glucose, insulin, glucagon-like peptide-1 (GLP-1), C-peptide, glycated hemoglobin (HbAlc), lipid metabolism, and oxidative stress. For this purpose, 50 rats were divided into five groups: normal control (NC), diabetic control (STZ), and STZ plus treatment groups (STZ + PY, STZ + EY, and STZ + allantoin). After treatment for one-month, there was a decrease in blood glucose: 385 ± 7 in STZ, 231 ± 3 in STZ + PY, 214 ± 11 in STZ + EY, and 243 ± 6 mg/dL in STZ + allantoin, respectively. There were significant statistical differences (p < 0.001) compared to STZ (100%): 60% in STZ + PY, 55% in STZ + EY, and 63% in STZ + allantoin. With groups in the same order, there were significant decreases (p < 0.001) in HbAlc (100% as 24.4 ± 0.6 ng/mL, 78%, 75%, and 77%), total cholesterol (100% as 122 ± 3 mg/dL, 70%, 67%, and 69%), and low-density lipoprotein (100% as 29 ± 1 mg/dL, 45%, 48%, and 38%). There were also significant increases (p < 0.001) in insulin (100% as 0.22 ± 0.00 ng/mL, 173%, 209%, and 177%), GLP-1 (100% as 18.4 ± 0.7 pmol/mL, 160%, 166%, and 162%), and C-peptide (100% as 2.56 ± 0.10 ng/mL, 129%, 132%, and 130%). The treatment effectively ameliorated antioxidant stress as shown by a significant decrease (p < 0.001) in malondialdehyde (100% as 7.25 ± 0.11 nmol/mL, 87%, 86%, and 85%) together with increases (p < 0.01) in superoxide dismutase (100% as 167 ± 6 IU/mL, 147%, 159%, and 145%) and reduced glutathione (100% as 167 ± 6 nmol/mL, 123%, 141%, and 140%). The results indicate that yam and allantoin have antidiabetic effects by modulating antioxidant activities, lipid profiles and by promoting the release of GLP-1, thereby improving the function of ß-cells maintaining normal insulin and glucose levels.
Subject(s)
Allantoin/therapeutic use , Blood Glucose/metabolism , Diabetes Mellitus, Experimental/drug therapy , Dioscorea/chemistry , Insulin/blood , Phytotherapy , Plant Extracts/therapeutic use , Allantoin/pharmacology , Animals , Antioxidants/metabolism , Antioxidants/pharmacology , Antioxidants/therapeutic use , C-Peptide/metabolism , Diabetes Mellitus, Experimental/blood , Glucagon-Like Peptide 1/blood , Glycated Hemoglobin/metabolism , Hypoglycemic Agents/pharmacology , Hypoglycemic Agents/therapeutic use , Lipoproteins, LDL/blood , Male , Oxidative Stress/drug effects , Plant Extracts/pharmacology , Rats, Sprague-Dawley , Superoxide Dismutase/metabolismABSTRACT
Magnesium (Mg) plays a central role in neuronal activity, cardiac excitability, neuromuscular transmission, muscular contraction, vasomotor tone, and blood pressure, all of which are significantly related to physical performance. To date, the available data about detection of blood total Mg (tMg; free-ionized, protein-bound, and anion-complex forms) are inconsistent, and there is limited information on blood free-ionized Mg (Mg(2+)) in relation to physical exercise. The aim of this study was to determine the biochemical changes related to energy metabolism after acute exhaustive swimming exercise (AESE) in rats in an attempt to correlate the role of blood Mg(2+) with metabolites/enzymes related to energy production. After AESE, blood Mg(2+), tMg, K(+), partial pressure of carbon dioxide, lactate, total protein (T-PRO), high-density lipoprotein (HDL), creatinine (CRE), blood urea nitrogen (BUN), uric acid (UA), alanine aminotransferase (ALT), aspartate aminotransferase (AST), alanine phosphatase (ALP), lactate dehydrogenase (LDH), and creatinine kinase (CK) were significantly increased, whereas pH, partial pressure of oxygen, oxygen saturation, the Mg(2+)/tMg and Ca(2+)/Mg(2+) ratios, HCO3 (-), glucose, triglyceride (TG), and low-density lipoprotein (LDL) were significantly decreased. During AESE, lactate, T-PRO, albumin, AST, ALP, LDH, CK, CRE, BUN, and UA showed significant positive correlations with changes in blood Mg(2+), while glucose, TG, and LDL correlated to Mg(2+) in a negative manner. In conclusion, AESE induced increases in both blood Mg(2+) and tMg, accompanied by changes in blood metabolites and enzymes related to energy metabolism due to increased metabolic demands and mechanical damages.
Subject(s)
Energy Metabolism/physiology , Enzymes/blood , Magnesium/blood , Physical Conditioning, Animal/physiology , Swimming/physiology , Alanine Transaminase/blood , Animals , Aspartate Aminotransferases/blood , Blood Glucose/metabolism , Blood Urea Nitrogen , Calcium/blood , Carbon Dioxide/blood , L-Lactate Dehydrogenase/blood , Lactic Acid/blood , Lipoproteins, LDL/blood , Male , Oxygen/blood , Rats, Sprague-Dawley , Triglycerides/blood , Uric Acid/bloodABSTRACT
BACKGROUND: In addition to being used to treat mental disorders, a serious complication of cancer, antidepressants have been reported to improve cancer patient immunity, inhibit cell growth and have an antitumor effect on various cancer cell lines. We investigated the apoptotic effect of fluoxetine against the Hep3B human hepatocellular carcinoma cell line. MATERIALS AND METHODS: After treatments of Hep3B cells with fluoxetine, we measured cell viability, reactive oxygen species (ROS), mitochondrial membrane potential (MMP) and activation of mitogen-activated protein kinases (MAPK). RESULTS: Fluoxetine reduced the viability of cancer cells, induced loss of MMP and formation of ROS, reduced expression of extracellular signal-regulated kinase 1/2 and increased expression of c-JUN N-terminal kinase and p38 MAPK. N-Acetylcysteine, an oxidant-scavenger, and 1,2-bis (o-aminophenoxy) ethane-N,N,N',N'-tetraacetic acid (BAPTA-AM), an intracellular Ca(2+) chelator, prevented fluoxetine-induced modulation of MAPK. CONCLUSION: Fluoxetine appears to exhibit an apoptotic effect against Hep3B cells through the loss of MMP, formation of ROS and modulation of MAPK activities.
Subject(s)
Apoptosis/drug effects , Carcinoma, Hepatocellular/pathology , Fluoxetine/pharmacology , Liver Neoplasms/pathology , Selective Serotonin Reuptake Inhibitors/pharmacology , Carcinoma, Hepatocellular/enzymology , Carcinoma, Hepatocellular/metabolism , Cell Line, Tumor , Humans , Liver Neoplasms/enzymology , Liver Neoplasms/metabolism , Matrix Metalloproteinases/metabolism , Mitogen-Activated Protein Kinases/metabolism , Reactive Oxygen SpeciesABSTRACT
In this study, we have used computational fluid dynamics to investigate the blood flow in the stenosed blood vessels. The numerical simulation using commercial software ADINA 8.6 were solved about the stenosed blood vessel according to the percent of stenosis and Reynolds number. The blood flow in the normal and stenosed blood vessel was grasped for the validity of the model. The characteristic of the pulsatile flow changed through the steady state flow and analysis of the pulsatile flow according to the time was grasped. The computational model with the characteristics of the fluid-structure interaction is introduced to investigate the wall shear stress, pressure distribution and axial flow velocity. The results show that axial flow velocity and wall shear stress in the region of stenosis was increased by increasing percent of stenosis and Reynolds number. Also, we can know that in the stenosed blood vessel the possibility of the generation of the aneurysm was increased by increasing Reynolds number and percent of stenosis.
Subject(s)
Aneurysm, Ruptured/physiopathology , Arterial Occlusive Diseases/physiopathology , Arteries/physiopathology , Models, Cardiovascular , Rheology/methods , Aneurysm, Ruptured/etiology , Animals , Arterial Occlusive Diseases/complications , Arterial Pressure , Blood Flow Velocity , Computer Simulation , Humans , Shear Strength , Vascular ResistanceABSTRACT
Panax ginseng (P. ginseng) has anti-cancer effects in several cancer models. Ginsenosides are the main bioactive components in P. ginseng. Korean red ginseng (KRG) extract can potently kill various cancer cells and ginsenosides Rg3 (GRg3) and Rh2 (GRh2) are the primary ginsenosides in KRG. This study was carried out to examine whether KRG and its primary ginsenosides (GRg3 and GRh2) affect apoptosis of human hepatocellular carcinoma cells (Hep3B). KRG, GRg3 and GRh2 have obvious cytotoxic and apoptotic effects in Hep3B cells as evidenced by a decrease in cell viability and mitochondria membrane potential, but an increase in LDH release. In the mitochondria-mediated apoptosis pathway, KRG, GRg3 and GRh2 have the ability to stimulate the release of mitochondrial cytochrome c, activation of caspase-3 and Bax protein, inhibition of Bcl-2 protein and production of intracellular reactive oxygen species in Hep3B cells. These results suggest that KRG, GRg3 and GRh2 may induce apoptosis by direct activation of the mitochondrial pathway.
Subject(s)
Apoptosis/drug effects , Carcinoma, Hepatocellular/metabolism , Ginsenosides/pharmacology , Mitochondria, Liver/drug effects , Reactive Oxygen Species/metabolism , Signal Transduction/drug effects , Blotting, Western , Carcinoma, Hepatocellular/pathology , Cell Line, Tumor , Humans , Liver Neoplasms/metabolism , Liver Neoplasms/pathology , Mitochondria, Liver/metabolismABSTRACT
Our previous study revealed that resveratrol blocks prion protein peptide PrP(106-126)-induced neurotoxicity. However, the mechanism of resveratrol-mediated neuroprotection in prion diseases is not clear. Resveratrol initiates neuroprotective effects via the activation of autophagy, which protects organelles, cells, and organisms against misfolded protein-disorders, including Alzheimer's disease and Parkinson's disease via regulation of mitochondrial homeostasis. Thus, we focused on elucidating the mechanisms responsible for resveratrol-mediated neuroprotection related to mitochondrial homeostasis as a result of autophagy activation. Resveratrol prevented PrP(106-126)-induced neuronal cell death by activating autophagy. Moreover, resveratrol-induced autophagy prevented the PrP(106-126)-induced reduction in mitochondrial potential and translocation of Bax to the mitochondria and cytochrome c release. Our results indicate that treatment with resveratrol appears to protect against neurotoxicity caused by prion protein peptides and the neuroprotection is induced by resveratrol-mediated autophagy signals.
Subject(s)
Autophagy/drug effects , Neuroprotective Agents/pharmacology , Peptide Fragments/toxicity , Prions/toxicity , Stilbenes/pharmacology , Autophagy/physiology , Cell Line, Tumor , Humans , Mitochondria/drug effects , Mitochondria/physiology , Peptide Fragments/antagonists & inhibitors , Prions/antagonists & inhibitors , ResveratrolABSTRACT
ETHNOPHARMACOLOGICAL RELEVANCE: Panax ginseng (P. ginseng) is one of the most widely used medicinal plants due to its wide spectrum of medicinal effects. Among the currently available Panax ginseng products, Korea red ginseng (KRG) has been shown to exhibit a variety of antioxidative and hepatoprotective action. AIM OF THE STUDY: Our aim was to investigate the effects of KRG and its primary ginsenosides (Rg3 and Rh2) on EtOH-induced injury to mouse hepatocytes (TIB-73). MATERIALS AND METHODS: We investigated the effects of KRG and its primary ginsenoside on EtOH-induced injury to TIB-73 cells and evaluated MAPKs signals as a possible mechanism of action. Hepatocytic injury was evaluated by biochemical assays as cell viability, lactate dehydrogenase (LDH), aspartate aminotransferase (AST), ROS and mitochondria membrane potential (MMP) level in TIB-73 cells. The levels of MAPK activation were analyzed by Western blots. RESULTS: The results showed that exposure of EtOH to TIB-73 cells led to cell death and membrane damage, accompanied by a decrease in cell viability, MMP, and Mg(2+) concentrations, but an increase in LDH, AST, ROS and MAPK activation. KRG and its primary ginsenosides reduced EtOH-induced generation of ROS and the activation of ERK and JNK, and increased Mg(2+) concentrations. CONCLUSION: These results suggest that KRG and its primary ginsenosides inhibit EtOH-induced oxidative injury by suppression of the MAPK pathway in TIB-73 cells.
Subject(s)
Antioxidants/pharmacology , Chemical and Drug Induced Liver Injury/metabolism , Ethanol/adverse effects , Ginsenosides/pharmacology , Mitogen-Activated Protein Kinases/antagonists & inhibitors , Panax , Animals , Cell Line , Cell Survival/drug effects , Chemical and Drug Induced Liver Injury/pathology , Chemical and Drug Induced Liver Injury/physiopathology , L-Lactate Dehydrogenase/metabolism , Magnesium/metabolism , Membrane Potential, Mitochondrial/drug effects , Mice , Mitogen-Activated Protein Kinases/metabolism , Oxidative Stress/drug effects , Plant Extracts/pharmacology , Reactive Oxygen Species/metabolismABSTRACT
BACKGROUND: Fructose-induced hypertension was used to test the hypothesis that taurine supplementation and/or exercise can prevent hypertension and increase exercise capacity. METHODS: Five groups of 15 Sprague-Dawley rats were allocated and designated as control, high fructose-fed (fructose), high fructose-fed plus exercise (FE), high fructose-fed plus 2% taurine supplement (FT) and high fructose-fed plus 2% taurine supplement and exercise (FET) groups. Noninvasive systolic blood pressure (SBP) was recorded weekly and invasive arterial blood pressure (ABP) was recorded at the end of the 4-week trial. Three consecutive swimming tests were performed in the selected rats from each group and the plasma biomarkers were measured in the remaining rats. RESULTS: Noninvasive SBP differed significantly (P < 0.001) from week 3, both noninvasive and invasive ABP increased significantly (P < 0.001), and exercise capacity significantly decreased (P < 0.001) in the fructose group compared with the control group. The individual effects of swimming and taurine supplementation were incapable of preventing the development of hypertension and SBP significantly (P < 0.001) increased in the FE and FT groups; exercise capacity in those groups remained similar to control. The combined effects of exercise and taurine alleviated hypertension and significantly increased exercise capacity in the FET group. Insulin resistance increased significantly and plasma nitric oxide (NO) decreased significantly in the F, FE, and FT groups. Both parameters remained similar to control values in the FET group with an increasing antioxidant activity. CONCLUSION: Taurine supplementation in combination with exercise prevents hypertension and increases exercise capacity by possibly antioxidation and maintaining NO concentrations.