ABSTRACT
We studied the role of calcium-regulating structures of slow- (m. soleus, SOL) and fast-twitch (m. extensor digitorum longus, EDL) skeletal muscles of rats during adaptation to exhausting physical activity and the possibility of modulating this adaptation with decaffeinated green tea extract. It was established that EDL adaptation is mainly aimed at Са2+ elimination from the sarcoplasm by Са-ATPase and its retention in the reticulum by calsequestrin. Administration of green tea extract increased endurance due to involvement of slow-twitch muscles whose adaptation is associated with enhanced expression of all the studied genes responsible for the regulation of Ca2+ balance.
Subject(s)
Calcium/metabolism , Muscle Fibers, Slow-Twitch/metabolism , Plant Extracts/pharmacology , Transcriptional Activation/drug effects , Animals , Camellia sinensis/chemistry , Drug Evaluation, Preclinical , Male , Metabolic Networks and Pathways , Muscle Fibers, Slow-Twitch/drug effects , Physical Conditioning, Animal , Rats, Wistar , TranscriptomeABSTRACT
Myocarditis is defined as myocardial inflammation, followed by cardiomyocyte necrosis. Diagnostics of myocarditis is based on unsafe and complicated method of endomyocardial biopsy (EMB). Development of myocarditis might alter gene expression not only in cardiac but in peripheral blood cells (PBC) as well. So, transcription profiles can be considered as possible biomarkers for the given pathology. At the moment, there are no reference genes defined for expression analysis in myocarditis studies. In this study, we analyzed mRNA content of six housekeeping genes in EMB and PBC samples. An algorithm for processing qPCR results obtained under the limited amount of sample is proposed. Set of GAPDH and HPRT1 genes has been selected for normalization of gene expression profiles in cardiac tissue and blood cells under the studies of myocarditis.