Measurement of the Pion Form Factor with CMD-3 Detector and Its Implication to the Hadronic Contribution to Muon (g-2).

The cross section of the process e^{+}e^{-}→π^{+}π^{-} has been measured in the center-of-mass energy range from 0.32 to 1.2 GeV with the CMD-3 detector at the electron-positron collider VEPP-2000. The measurement is based on an integrated luminosity of about 88 pb^{-1}, of which 62 pb^{-1} represent a complete dataset collected by CMD-3 at center-of-mass energies below 1 GeV. In the dominant region near the ρ resonance a systematic uncertainty of 0.7% was achieved. The implications of the presented results for the evaluation of the hadronic contribution to the anomalous magnetic moment of the muon are discussed.

Isolation and Characterization of Salmonella Bacteriophages as Potential Agents for Phage Therapy of Antibiotic-Resistant Intestinal Infections.

Two bacteriophages specifically active against to pathogenic strains of the Salmonella genus were isolated. The morphology of phage colonies (size, transparency, and shape of the plaque edge, and halo) and the spectrum of their lytic activity and interaction with microbial cells (adsorption rate, duration of the latency, and reproductive efficiency) were examined. Using genome-wide sequencing, we determined the taxonomic position of bacteriophages and verified the absence of unwanted genes encoding toxins, adhesins, and invasins, as well as pathogenicity islands responsible for antibiotic resistance. In addition, phage stability under different physical conditions and their productivity were studied.

[FEATURES OF THE DEVELOPMENT OF THE REPRODUCTIVE SYSTEM BY USING GENETICALLY MODIFIED SOURCES (EXPERIMENTAL STUDY)].

The rapid development of molecular biology and genetic engineering contributes to the creation of plants with desired properties in a short time. One of the aspects of the study of genetically modified organisms (GMO) and genetically modified products (GMP) is the study of their impact on humans, animals and the environment. Subject of research - changes in the morph functional indicators of the reproductive system of mice. The relevance of the chosen topic is due to the importance of the reproductive system for the reproduction of a healthy generation, capable of developing normally and continuing its race. Purpose of the study - to identify the effect of GMOs on the reproductive system of mice. Cultivation of three groups of laboratory mice using transgenic feed (genetically modified soybean meal) and obtaining biological material for research. Determination of the presence of genetically modified sources (GMS), genetically modified organisms (GMO) in food and feed products using the polymerase chain reaction (PCR). Morphometric study of the obtained material and biometric data processing. It has been shown that the consumption of feed by animals prepared on the basis of genetically modified plants does not affect the reproductive functions of the parental generation; but at the same time there was an inhibition of the growth rate and the process of formation of the gonads of the descendants of the first and, especially, the second generation; the second generation of offspring, eating only soybean meal, had defective reproductive qualities and high mortality. Second-generation mice eating genetically modified soybean meal are at greater risk than second-generation mice eating traditional diets.

[Endoscopic treatment of benign gastrointestinal fistulas]. / Vozmozhnosti éndoskopicheskogo metoda v lechenii svishchei zheludochno-kishechnogo trakta neopukholevogo geneza.

OBJECTIVE: To evaluate the possibility and safety of modified endoscopic stent in the treatment of benign intestinal fistulas. MATERIAL AND METHODS: Analysis of the experience of Sklifosovsky Research Institute for Emergency Care and recent numerous foreign reports confirms that staged treatment followed by delayed radical surgery is the most perspective approach. Modified endoscopic treatment of intestinal fistulas successfully used in 10 patients is reported in the article. RESULTS: Endoscopic stenting of various parts of gastrointestinal tract is a minimally invasive treatment of this pathology and not followed by complications and mortality. An important advantage is early closure of fistula that reduces duration of treatment and improves further social and labor rehabilitation of patients.

THE INFLUENCE OF THE PHYSICAL ACTIVITY OF A MODERN STUDENT ON THE CHARACTERISTICS OF THE CARDIOVASCULAR AND RESPIRATORY SYSTEMS AND THEIR RESISTANCE TO STRESS DURING EDUCATIONAL PROCESS.

Many students in the educational process are subject to emotional, cognitive, motivational stress. A little physical activity allows them to get out of this state. One aspect of the research of adaptation of the students to the educational and physical loads is the investigation of their cardiovascular and respiratory systems state. There were investigated 100 students (educational directions: "Physical culture", "Music", "General Medicine") (age 19-24) from Kazakhstan, Russia, Turkey with different levels of physical activity in the process of their adaptation to educational process Anthropometric measurements were used to assess the parameters of physical development. The method of variational pulsometry by R.M. Baevskiy and spirometry were used to assess the functional state of the both systems. Stress resistance to learning and physical loads was determined due to assess the indices of general aerobic and speed-strength anaerobic endurance, survey among students. It was shown that students with a low level of physical activity are characterized by a pronounced predominance of the sympathoadrenal chain of heart rhythm regulation, which indicates a significant stress of adaptation mechanisms. The effectiveness of the regulation of heart rate in students of the educational direction "Physical Culture" is higher, which indirectly proves the positive impact of systematic physical activity on the functioning of the cardiovascular system and the body as a whole. It is advisable to solve the problem of the lack of physical activity of students by including various forms of physical education in their educational everyday activities that can be regulated daily by teachers. At the same time, the systemic nature of the physical impact on the student body is ensured by the interconnection of all forms of physical activity and types of motor activity, the content and direction of which is constantly updated considering the hierarchy of goals.

Spin Dependence of η Meson Production in Proton-Proton Collisions Close to Threshold.

Taking advantage of the high acceptance and axial symmetry of the WASA-at-COSY detector, and the high polarization degree of the proton beam of COSY, the reaction p[over →]p→ppη has been measured close to threshold to explore the analyzing power A_{y}. The angular distribution of A_{y} is determined with the precision improved by more than 1 order of magnitude with respect to previous results, allowing a first accurate comparison with theoretical predictions. The determined analyzing power is consistent with zero for an excess energy of Q=15 MeV, signaling s-wave production with no evidence for higher partial waves. At Q=72 MeV the data reveal strong interference of Ps and Pp partial waves and cancellation of (Pp)^{2} and Ss^{*}Sd contributions. These results rule out the presently available theoretical predictions for the production mechanism of the η meson.

Use of nanotechnology in the creation of moden medicines-governmental funds and their targeting systems (literature review).

Properties of nanoscale carriers recommended by the European Pharmacopeia for use in the creation of drugs and their complexes with physiologically active substances are given. Ihe authors present general descriptions of the methods of creation and especially of carriers identified in the accumulation of experience in their use for creating drugs with desired properties of penetration of biological barriers and length of service of the therapeutic effect.

Exploring photochemistry of p-bromophenylsulfonyl, p-tolylsulfonyl and methylsulfonyl azides by ultrafast UV-pump-IR-probe spectroscopy and computations.

The photochemistry of p-bromophenylsulfonyl azide (BsN3), p-tolylsulfonyl azide (TsN3) and methylsulfonyl azide (MsN3) was studied by femtosecond time-resolved infrared spectroscopy with CH2Cl2 and CCl4 as solvents along with quantum chemical calculations. The photolysis of these azides after 267 nm light excitation leads to the population of each respective azide S1 excited state. Decay of the S1 excited state gives rise to singlet nitrene formation. In the case of BsN3, the decay was found to correlate with the formation of a pseudo-Curtius photoproduct (PCP) BrC6H4NSO2. Transient electronic ground states of the three azides on their way to singlet nitrenes and PCPs were shown by locating the corresponding transition states on the potential energy surfaces. The lifetime of singlet (1)(BsN) and (1)(TsN) nitrenes is τ(S) = ∼20 ps in CH2Cl2 and ∼700 ps in CCl4. Singlet (1)(MsN) was not detected. Due to fast intersystem crossing (ISC), singlet nitrenes are converted into the triplet spin isomers lying lower in energy, the formation time constants being equal to the corresponding singlet nitrene lifetime. The formation of (3)(MsN) was shown and the formation time constant in CH2Cl2 was found to be τ(ISC) = 34 ± 3 ps. Internal conversion of the S1 excited state to the ground state of the azide was low (Φ ≈ 0.15) for BsN3 and TsN3 and was not found in the case of MsN3.

[Influence of the antihypoxantic therapy on children with generalized peritonitis]. / Vliyanie antigipoksantov na rezul'taty lecheniya rasprostranennogo peritonita u detei.

AIM: To improve the results of treatment of a widespread purulent peritonitis in children by optimizing fluid therapy includes the use of combined treatment: reamberin and remaxol. MATERIAL AND METHODS: We studied 269 patients aged 1 to 15 years with a widespread purulent peritonitis treated at the children's surgical departments in Samara from 2001 to 2015. The study group included 179 children who used the optimized infusion therapy. In the study group was allocated to 2 groups: 69 children in infusion therapy which used reamberin and 110 patients in which treatment was applied reamberin and remaxol. The surgical treatment used laparoscopic sanation of the abdomen. Comprehensive survey included a study of dynamics of the white blood cell count, leukocyte index Kalf-Caliph, erythrocyte sedimentation rate, temperature, total albumin concentration, transaminase levels. Catamnesis studied 48 patients with the definition of complex intima-media thickness in the projection of basilar, brachial and femoral arteries. RESULTS: A study compared indicators of both groups, revealed a more rapid reduction of intoxication symptomps (leukocytosis, LII, body temperature), the disappearance of enteric disease, recovery of protein-synthetic function of the liver, decrease of cytolytic and mesenchymal-inflammatory syndromes in the main group, especially in the subgroup in which treatment was included remaxol. CONCLUSION: The use of reamberin and remaxol in infusion therapy led to improvement of the results of the treatment of common purulent peritonitis in children. Study catamnesis with the study of the intima-media revealed that children undergoing widespread purulent peritonitis further develop signs of endothelial dysfunction. The developed clinical recommendations to significantly reduce the risk of developing signs of endothelial dysfunction, thereby reducing the possible appearance of vascular pathology in patients who underwent childhood widespread purulent peritonitis.

[Treatment of diffuse peritonitis in children].

THE PURPOSE OF THE STUDY: Improving the treatment of advanced peritonitis via use in therapy antihypoxant Reamberin and hepatoprotector Remaxol, nutritional support, sanitation laparoscopic abdominal cavity. SUBJECTS: A total of 232 children aged 1 to 15 years with generalized purulent peritonitis treated at the children's surgical departments of Samara from 2001 to 2014. A study group comprised 148 patients who used the optimized pathogenetic therapy. In the study group was allocated two groups: 64 patients in the pathogenetic therapy that used antihypoxant reamberin, and 84 children in the treatment of which reamberin and hepatoprotector remaxol. All the children of the main group received nutritional support (trophic feedings), used in the surgical treatment of abdominal laparoscopic sanitation. Comprehensive survey includes the study of the dynamics of the level of white blood cells, leukocyte index on Kalf-Caliph, erythrocyte sedimentation rate, temperature, total albumin concentration, transaminase levels. RESULTS: Comparison of the studied parameters in the study and control groups, showed a more rapid decrease in the symptoms and signs of intoxication (leukocytosis, LII, body temperature), relief of enteric disease, recovery of protein-synthetic function of the liver, a decrease of cytolytic and mesenchymal-inflammatory syndrome in the study group, especially in the subgroup in which therapy was included remaxol. CONCLUSIONS: Optimization of treatment involving the application of the combined drugs--antihypoxant reamberin, hepatoprotector remaxol, nutritional support and implementation of laparoscopic abdominal sanitation led to improved results of therapy common purulent peritonitis in children.

[TESTING THE PROTECTIVE PROPERTIES OF ARGON DURING SUBACUTE EXPOSURE TO HYPOXIC HYPOXIA].

Previously it has been shown that argon has a stimulating effect on biological processes including, specifically, strong protective properties during exposure to acute (1-4 hrs.) hypoxic hypoxia. Experiments with animals under hypoxia gradually growing over 3 to 7 days may give insight into mechanisms of the argon protective action. Purpose of the work was to explore character and severity of developing disorders, to attempt their prognosis and to test potentiality of argon as a protector from the effects of subacute hypoxic exposure. In two series of experiments Wistar male rats were housed in pressure chambers with different rates of O2 reduction without argon and with argon in concentration of 32-38%. Oxygen reduction from 20.9 to 9.0% in 3 days led to the death of 50% animals. Oxygen reduction to 12.0% in 7 days and one day in low-oxygen atmosphere was harmless for animals. Argon in air makes the response to hypoxia almost twice as sharp on virtually every level (red blood system, physiological, morphological and biochemical shifts) validating the concept that argon acts as a stimulator. In context of the most critical processes of adaptation to hypoxia--activation of blood formation and lipid metabolism--this sharpening of the response results in depletion of body resources and instigates irreversible shifts.

Neodymium glass laser with a phase conjugate mirror producing 220 J pulses at 0.02 Hz repetition rate.

For pumping multipetawatt Ti:sapphire laser facilities we developed a compact repetitively pulsed laser based on neodymium phosphate glass with pulse energy of 220 J, pulse repetition rate of 0.02 Hz, beam diameter of 43 mm, aperture fill factor of 0.8, and FWHM pulse duration of 30 ns. The phase distortions of laser radiation were compensated by optical phase conjugation via stimulated Brillouin scattering. The depolarization was reduced to 0.4% using linear compensation methods. The beam quality was 2.5 x diffraction limit (150 µrad).

Diagnostic and remedial capability of transcervical falloposcopy in conjunction with laparoscopy.

STUDY OBJECTIVE: Tubal factor accounts for 25-30% of cases of female infertility. Laparoscopy "Gold Standard" for tubal evaluation. However, it is known that during the initial infection of the fallopian tube mucosal damage occurs, a condition which plays a decisive role in reproduction. MATERIALS AND METHODS: In this prospective randomized study, 468 infertile women with evidence of fallopian tube disease were included. In this, for 256 patients (group 1) after laparoscopic salpingolysis, salpingostomy we performed an additional step operation transcervical falloposcopy tubal dilatation (TFTD). 212 patients (group 2) produced only laparoscopic salpingolysis, salpingostomy. RESULTS: As a result, TFTD patency of the fallopian tubes for coaxial catheter was restored in 50 (78%) of 64 tubes with bilateral total occlusion, in 238 (93%) of 254 with partial occlusion of the bilateral, in 14 (58%) of 24 total unilateral occlusion and 26 (92%) of 28 with partial unilateral occlusion. Total number of pregnancies for one year in the first group of patients was 152 (59.3%), in the second 57 (27.1%), of which in the first group 147 -intrauterine pregnancies (57.4%) and in the second - 46 (21.7%). CONCLUSION: Falloposcopy surgeon provides good opportunities for the diagnosis and treatment of intralumen lesions. Significant increase in the frequency of uterine pregnancy in the case of an additional step - TFTD during salpingolysis, salpingostomy in patients with tubal factor infertility.

[The history of the institute of higher and additional professional education of the Endocrinology Reserch Centre].

The Endocrinology Reserch Centre is proud not only of its achievements in the area of personalized approach to the examination and treatment of patients in accordance with the principles of evidence-based medicine, the use of modern diagnostic and treatment technologies, but also of its rich history of training scientific and medical personnel. For many years the Center has been attracting the best and most talented graduates of higher medical institutions, becoming the "alma mater" for young doctors - endocrinologists, pediatric endocrinologists and nutritionists. Specialists, graduates of NMRC, make a significant contribution to the development of medicine, conducting research, creating innovative methods of treatment and helping patients not only in our country, but also abroad. However, the State Research Center of the Russian Federation FGBU «NMRC Endocrinology¼ is famous not only for its high-quality and comprehensive training, but also for its experienced, dedicated teaching staff, giving students unique opportunities for professional growth. The purpose of this article is to reflect the most important milestones in the development of education at NMIC Endocrinology, which began its formation more than a century ago, during the most difficult period of transition for our country.

Synchrotron single-crystal XRD, IR-, Raman spectroscopy and high pressure study of synthetic krieselite.

Spontaneous crystals of krieselite (Ge analogue of topaz) with the chemical formula Al2[(Ge0.75Si0.25)O4](F1.63OH0.37) were synthesized using a thermo-gradient hydrothermal method at a temperature of 600/650 °C and pressure of 100 MPa. The unit cell parameters are: a = 8.9732(8) Å, b = 8.4823(7) Å, c = 4.7379(5) Å, V = 360.62(6) Å3, space group Pnma. The F-/OH- content of the samples was refined by FTIR spectroscopy method. Raman spectroscopy showed the main differences between the spectra of krieselite and topaz at the ambient conditions. The assignment of observed and calculated Ag bands (cm-1) for non-polarized Raman spectra was carried out. Using in situ Raman spectroscopy at high pressures, the dependence of the shift in the position of the main bands of the krieselite Raman spectrum on the pressure was established, and the corresponding paths of pressure induced distortion of crystal structure was assumed. According to the data of Raman spectroscopy, it was revealed that krieselite does not undergo the phase transitions up to 30 GPa. The probable way of crystal structure distortion within the space group Pnma was proposed based on simulation of high-pressure Raman spectra.

Effects of reversible inhibition of cholinesterase and nicotine on mouse mortality and blood levels of proinflammatory cytokines during the early phase of sepsis.

Experiments on outbred albino mice have shown that proserine (reversible cholinesterase inhibitor) and nicotine (nicotinic receptor agonist) in a equivalent dose of 0.2 DL(50)injected 2 h before sepsis induction significantly reduced animal mortality from experimental infection due to reduction of blood concentrations of proinflammatory cytokines TNF-α, IL-1ß, and IL-6.

Role of nicotinic and muscarinic cholinoreceptors in the realization of the cholinergic anti-inflammatory pathway during the early phase of sepsis.

Stimulation of nicotinic and muscarinic cholinoreceptors (nAChR, mAChR) in outbred albino mice with nicotine and aceclidine, respectively, in single equilethal doses 0.5 DL(50)6 h before sepsis induction significantly reduced animal mortality due to a decrease in blood concentrations of proinflammatory cytokines IL-1ß, IL-6, and MIP-2. Stimulation of mAChR (injection of aceclidine) stimulated the neutrophilic phagocytic and metabolic activity. Realization of the cholinergic anti-inflammatory pathway (stimulation of the peripheral nicotinic cholinoreceptors (α7nAChR) and central muscarinic cholinoreceptors (mAChR) was modulated by stimulation of the muscarinic cholinoreceptors of the phagocytic monocytic system cells.

Structural insights into light-driven anion pumping in cyanobacteria.

Transmembrane ion transport is a key process in living cells. Active transport of ions is carried out by various ion transporters including microbial rhodopsins (MRs). MRs perform diverse functions such as active and passive ion transport, photo-sensing, and others. In particular, MRs can pump various monovalent ions like Na+, K+, Cl-, I-, NO3-. The only characterized MR proposed to pump sulfate in addition to halides belongs to the cyanobacterium Synechocystis sp. PCC 7509 and is named Synechocystis halorhodopsin (SyHR). The structural study of SyHR may help to understand what makes an MR pump divalent ions. Here we present the crystal structure of SyHR in the ground state, the structure of its sulfate-bound form as well as two photoreaction intermediates, the K and O states. These data reveal the molecular origin of the unique properties of the protein (exceptionally strong chloride binding and proposed pumping of divalent anions) and sheds light on the mechanism of anion release and uptake in cyanobacterial halorhodopsins. The unique properties of SyHR highlight its potential as an optogenetics tool and may help engineer different types of anion pumps with applications in optogenetics.

Synthetic brunogeierite Fe2GeO4: XRD, Mössbauer and Raman high-pressure study.

We present complex spectroscopic data of the synthetic brunogeierite (Fe2+)2Ge4+O4 with space group Fd3¯m of spinel structure: a = 8.3783 (6) Å, V = 588.12 (13) Å3. Brunogeierite crystals up to 200 µm in size were crystallized by the interaction of a boric acid solution on a metal iron wire in the presence of germanium oxide (GeO2) at 600/650 °C and 100 MPa. Mössbauer spectrum of synthetic brunogeierite consists of the symmetric doublet with the parameters IS = 1.104(1) mm/s and QS = 2.845(1) mm/s, that corresponds to the octahedral position of iron ions ([VI]Fe2+). The Raman spectra of Fe2GeO4 crystal consist of an intense main band at 756 cm-1 and four less intense bands at ∼644, 472, 302, and ∼205 cm-1 at ambient conditions. All five bands inherent in the spectrum of cubic spinel are present and gradual change in high pressure spectra up to 30 GPa. The color of the crystal changes from brown-orange to reddish at the center at 22.7 GPa and became opaque black up to 30.2 GPa. Herewith, in the high pressure spectra, we observed the splitting of some bands and the appearance of additional bands in a wide pressure range (from 1.6 to 30 GPa). The factor group analysis with the lattice dynamics calculation of potential crystal structure distortions shows the decreasing of the structure symmetry to tetragonal or rhombohedral in this pressure range.