Association of high-fat diet with neuroinflammation, anxiety-like defensive behavioral responses, and altered thermoregulatory responses in male rats.

Overweight and obesity are a worldwide pandemic affecting billions of people. These conditions have been associated with a chronic low-grade inflammatory state that is recognized as a risk factor for a range of somatic diseases as well as neurodevelopmental disorders, anxiety disorders, trauma- and stressor-related disorders, and affective disorders. We previously reported that the ingestion of a high-fat diet (HFD; 45% fat kcal/g) for nine weeks was capable of inducing obesity in rats in association with increased reactivity to stress and increased anxiety-related defensive behavior. In this study, we conducted a nine-week diet protocol to induce obesity in rats, followed by investigation of anxiety-related defensive behavioral responses using the elevated T-maze (ETM), numbers of FOS-immunoreactive cells after exposure of rats to the avoidance or escape task of the ETM, and neuroinflammatory cytokine expression in hypothalamic and amygdaloid nuclei. In addition, we investigated stress-induced cutaneous thermoregulatory responses during exposure to an open-field (OF). Here we demonstrated that nine weeks of HFD intake induced obesity, in association with increased abdominal fat pad weight, increased anxiety-related defensive behavioral responses, and increased proinflammatory cytokines in hypothalamic and amygdaloid nuclei. In addition, HFD exposure altered avoidance- or escape task-induced FOS-immunoreactivity within brain structures involved in control of neuroendocrine, autonomic, and behavioral responses to aversive stimuli, including the basolateral amygdala (BLA) and dorsomedial (DMH), paraventricular (PVN) and ventromedial (VMH) hypothalamic nuclei. Furthermore, rats exposed to HFD, relative to control diet-fed rats, responded with increased tail skin temperature at baseline and throughout exposure to an open-field apparatus. These data are consistent with the hypothesis that HFD induces neuroinflammation, alters excitability of brain nuclei controlling neuroendocrine, autonomic, and behavioral responses to stressful stimuli, and enhances stress reactivity and anxiety-like defensive behavioral responses.

Nutritional evaluation of the legume Macrotyloma axillare using in vitro and in vivo bioassays in sheep.

This study consisted of two experiments with the following objectives: to evaluate the effects of tannins from the tropical legume macrotiloma (Macrotyloma axillare) on total gas and methane (CH4 ) production, as well as on ruminal fermentation parameters by performing an in vitro bioassay, with samples incubated with and without polyethylene glycol (PEG) in a semi-automatic system; and secondly in a 17 day in vivo experiment, to determine apparent total tract digestibility (ATTD) of dietary nutrients and ruminal fermentation parameters of 12 intact 8- to 9-month-old Santa Inês (averaging 24.95 ± 1.8 kg body weight) ewes fed tropical grass hay supplemented with macrotiloma hay. The ewes were divided into two treatment groups depending on their diet: chopped aruana grass hay (Panicum maximum cv. Aruana) (control-CON); and aruana grass hay supplemented with chopped macrotiloma hay (macrotiloma-MAC). The animals were kept for 5 consecutive days in metabolic cages for the ATTD assay, and at the end of this period, samples of rumen fluid were collected from each ewe to determine ammoniacal nitrogen (NH3 -N) and short-chain fatty acid (SCFA) production, and protozoa count. For the in vitro assay, a decrease in total gas and CH4 production was observed for samples incubated without PEG (p < .05). No differences were observed for the other parameters evaluated (p > .05). In the in vivo experiment, increased intake and ATTD of crude protein were observed for the animals fed MAC when compared to CON (p < .05). For rumen fermentation parameters, increased NH3 -N, total SCFA and isobutyrate concentrations, as well as reduced protozoa count were observed for MAC when compared to CON (p < .05). The results observed here indicated the potential of macrotiloma for use as a ruminant feed, and antimethanogenic potential of this plant was noted.

Wheat gluten intake increases weight gain and adiposity associated with reduced thermogenesis and energy expenditure in an animal model of obesity.

BACKGROUND/OBJECTIVES: The association between gluten and body weight is inconsistent. Previously, we showed that a gluten-free diet reduces weight gain without changing food intake in mice fed high-fat diets. In the present study, we investigated the effects of gluten intake on fat metabolism, thermogenesis and energy expenditure in mice fed a standard or high-fat diet. METHODS: Mice were fed four different experimental diets during 8 weeks: a control-standard diet (CD), a CD added with 4.5% of wheat gluten (CD-G), a high-fat diet (HFD) and a HFD added with 4.5% of wheat gluten (HFD-G). After 8 weeks, the mice received (99m)Tc-radiolabeled gluten orally to study gluten absorption and biodistribution or they underwent indirect calorimetry. After killing, subcutaneous and brown adipose tissues (SAT and BAT) were collected to assess thermogenesis-related protein expression. Lipid metabolism was studied in adipocyte cultures from the four groups. RESULTS: Despite having had the same energy intake, CD-G and HFD-G mice exhibited increased body weight and fat deposits compared with their respective controls. (99m)Tc-GLU or its peptides were detected in the blood, liver and visceral adipose tissue, suggesting that gluten can even reach extraintestinal organs. Uncoupling protein-1 expression was reduced in the BAT of HFD-G and in the SAT of CD-G and HFD-G mice. Indirect calorimetry showed lower oxygen volume consumption in CD-G and HFD-G groups compared with their controls. In HFD mice, daily energy expenditure was reduced with gluten intake. Gluten also reduced adiponectin, peroxisome proliferator-activated receptor (PPAR)-α and PPARγ and hormone-sensitive lipase in cultures of isolated adipocytes from HFD mice, whereas in the CD-G group, gluten intake increased interleukin-6 expression and tended to increase that of tumor necrosis factor. CONCLUSIONS: Wheat gluten promotes weight gain in animals on both HFD and CD, partly by reducing the thermogenic capacity of adipose tissues.

Abdominal TRPV1 channel desensitization enhances stress-induced hyperthermia during social stress in rats.

AIMS: In rats, stress-induced hyperthermia caused by social interaction depends on brown adipose tissue (BAT) thermogenesis and peripheral vasoconstriction. However, the peripheral mechanisms responsible for regulating the level of hyperthermia during social stress are still unknown. The transient receptor potential vanilloid 1 (TRPV1) subfamily, expressed in sensory and visceral neurons, can serve as a thermoreceptor. Here, we tested the hypothesis that the abdominal TRPV1 is essential in regulating stress-induced hyperthermia during social stress. MAIN METHODS: Male Wistar rats received an intraperitoneal injection of Resiniferatoxin (RTX) - an ultra-potent capsaicin analog, (i.e., to desensitize the TRPV1 channels) or vehicle. Seven days later, we evaluated the effects of abdominal TRPV1 channels desensitization on core body temperature (CBT), brown adipose tissue (BAT) temperature, tail skin temperature, and heart rate (HR) of rats subjected to a social stress protocol. KEY FINDINGS: We found abdominal TRPV1 desensitization increased CBT and BAT temperature but did not change tail skin temperature and HR during rest. However, under social stress, we found that abdominal TRPV1 desensitization heightened the increase in CBT and BAT caused by stress. Also, it abolished the increase in tail skin temperature that occurs during and after social stress. TRPV1 desensitization also delayed the HR recovery after the exposure to the social stress. SIGNIFICANCE: These results show that abdominal TRPV1 channels desensitization heightens stress-induced hyperthermia, causing heat dissipation during and after social stress, enabling optimal thermal control during social encounters.

Sequential method for fast neural population activity reconstruction in the cortex from incomplete noisy measurements.

During recent years there has been a growing interest in stochastic dynamic neural fields employed for modeling and predictions in biomedical and technical systems. In this paper, given some incomplete noisy data available from sensors, we propose and explore a state estimation method for fast restorations of membrane potential in the cortex based on such measurements and the Amari equation used for simulations of neural population activity in a stochastic setting. Our novel technique relies upon a Galerkin-type spectral approximation utilized within the conventional state-space approach. Translating a stochastic system into its state-space form creates a straightforward and fruitful way to the data-driven parameter estimation, filtering, prediction and smoothing. The present study is particularly focused on establishing a nonlinear stochastic Galerkin-spectral-approximation-induced system of large size, which is further estimated by the traditional extended Kalman filter (EKF). The efficiency of calculations is the main purpose of our research. That is why the fast filtering solution devised is based on processing the incoming data incrementally, that is, by processing measurements one at a time, rather than handling them as a unified high-dimensional vector. Such sequential filters suit well for dealing with large data sets as well as with real-time on-line computations. Also, their derivation and substantiation is of great interest in the context of neural network training because of large stochastic systems arisen there. In comparison to the batch filtering, our novel algorithm reduces the computational cost of membrane potential reconstructions in terms of the amount of grid nodes N accepted in the underlying spacial discretization, significantly. Apart from its computation efficiency, this sequential method is more robust to round-off errors committed within a computer-based finite precision arithmetics than the classical EKF because of the (N × N)-matrix inversion elimination from such membrane potential calculations. The superior performance of our technique is examined and confirmed in comparison to the batch one on two known scenarios in the dynamic neural field modeling.

Application of Teledermoscopy in the Diagnosis of Pigmented Lesions.

BACKGROUND: Dermatology, due to the peculiar characteristic of visual diagnosis, is suitable for the application of modern telemedicine techniques, such as mobile teledermoscopy. OBJECTIVES: To evaluate the feasibility and reliability of the technique for the diagnosis of pigmented lesions. METHODS: Through the storage and routing method, 41 pigmented lesions were analyzed. After the selection of the lesions during the outpatient visit, the clinical and dermatoscopic images were obtained by the resident physician through the cellphone camera and sent to the assistant dermatologist by means of an application for exchange of messages between mobile platforms. Firstly, the assistant dermatologist described the visualized dermatoscopic structures and defined its diagnosis and conduct, based solely on the evaluation of the clinical and dermatoscopic images, without having the knowledge of the anamnesis data. Afterwards, the same assistant dermatologist evaluated the patient face to face, defining the dermatoscopic structures, diagnosis, and conduct. The data obtained through teledermoscopy and face-to-face assessments were compared and accuracy was defined as the concordance between the diagnoses. RESULTS: A match rate of 90% between teledermoscopic and face-to-face diagnosis was demonstrated (McNemar's statistical analysis, whose p value was 0.1366, showed no evidence to support the inferiority of the teledermoscopic method).

A goal-driven neural propositional interpreter.

This work presents ARQ-PROP-II, the propositional version of a neural engine for finding proofs by refutation using the Resolution Principle. This neural architecture does not require special arrangements or modules to do forward or backward reasoning, being driven by the goal posed to it. ARQ-PROP-II is capable of integrated monotonic reasoning with complete and incomplete knowledge. The neural mechanism presented herein is the first to our knowledge that does not require that the knowledge base be either pre-encoded or learnt.

Interleukin-6 expression and gene polymorphism are associated with severity of periodontal disease in a sample of Brazilian individuals.

Interleukin (IL)-6 is an inflammatory mediator involved in bone resorption. G/C polymorphism at position -174 of the IL-6 gene has been reported to influence IL-6 expression, with the G allele associated with higher expression levels. The aims of this study were to investigate the expression of IL-6 as well as the incidence of IL-6 (-174) gene polymorphism and their correlation to the severity of periodontitis in Brazilians. Peripheral blood mononuclear cells were collected from 12 non-smoker individuals with periodontitis for evaluation of IL-6 expression using flow cytometry. We observed a positive correlation between the mean clinical attachment loss and intensity of expression of IL-6, in which the greater the attachment loss, the higher the expression of IL-6 (P=0 x 007, R2=0 x 52). Also, patients with severe periodontitis displayed a higher intensity of IL-6 expression compared to moderate periodontitis (P=0 x 04). To determine the occurrence of IL-6 gene polymorphism, DNA was obtained from oral swabs of 209 Brazilian individuals with and without periodontitis. Polymerase chain reaction, restriction endonuclease digestion and electrophoresis were performed, allowing for detection of the IL-6 (-174) polymorphism. We observed that non-smokers with moderate periodontitis (P=0 x 05) and control (P=0 x 04) groups displayed a higher incidence of the G genotype when compared to severe periodontitis. This suggests that the G genotype may represent a protective role in severity of periodontitis. Thus, the increased expression of IL-6 and IL-6 (-174) polymorphism are associated with periodontal disease severity in Brazilian individuals.

Estudo clínico multicêntrico com duas doses diárias de 500 mg de naproxeno no tratamento de 172 pacientes portadores de osteoartrose / Multicentric clinic trial with 2 diary doses of 500 mg of naproxen in the treatment of 172 patients with osteoarthritis

Os autores apresentam os resultados obtidos num ensaio multicêntrico com o uso do ácido d-2(6'metoxi-2'naftil) propiônico - naproxeno - no tratamento de 172 pacientes portadores de osteoartrose, na dose de 1,0 g diária, fracionada em duas tomadas de 500 mg a intervalos de 12 horas. A duraçäo do estudo foi de 6 semanas, sendo as avaliaçöes clínicas individuais realizadas a cada duas semanas. Os parâmetros clínicos observados foram a dor, a limitaçäo dos movimentos e o edema articular. Todos os pacientes apresentavam dor, 149 tinham limitaçäo dos movimentos e 47 tinham edema articular. A resposta terapêutica no controle da dor foi excelente em 57 pacientes (33,14%), muito boa em 64 pacientes (37,21%), boa em 21 (12,21%), regular em 11 (6,40%) e insatisfatória em 19 (11,04%); a reduçäo da limitaçäo dos movimentos articulares foi excelente em 52 pacientes (34,90%), muito boa em 34 (22,82%), boa em 28 (18,79%), regular em 10 (6,71%) e insatisfatória em 25 (16,78%) e os resultados quanto à reduçäo do edema articular foram de 23 casos excelentes (48,94%), 12 casos muito bons (25,53%), bons em 7 14,89%), 1 regular (2,13%) e 4 insatisfatórios (8,51%). A avaliaçäo final dos resultados pelo investigador e pelo paciente ocorreu sem discrepância significativa: houve 46 resultados excelentes (26,76%), 59 muito bons (34,30%), 29 bons (16,87%), 14 regulares (8,13%) e 14 insatisfatórios (8,13%). Os resultados terapêuticos beneficiaram 134 pacientes (77,93%). Das 42 citaçöes de efeitos colaterais, 23 foram leves, 9 moderados e intensos em 10 pacientes (5,81%), que determinaram a suspensäo do medicamento. Mais freqüentes foram as manifestaçöes digestivas como náuseas, vômitos, dores abdominais e pirose. Os autores concluem ser o naproxeno muito eficaz no tratamento da osteoartrose, por apresentar excelente atividade antiinflamatória e analgésica, com muito boa tolerância