ABSTRACT
The sensitivity of enzyme kinetic substrate determinations can be improved with the aid of competitive inhibitors. As an example, the determination of glucose dehydrogenase in the presence of potassium thiocyanate is described. The method has the advantage of rapid operation with satisfactory precision.
Subject(s)
Alcohol Oxidoreductases/antagonists & inhibitors , Blood Glucose/analysis , Evaluation Studies as Topic , Hexokinase/metabolism , Humans , Kinetics , Microchemistry , Spectrophotometry, Ultraviolet/methods , ThiocyanatesABSTRACT
For fixed-time rate analysis, the turbidity curve, which is the reference system of immunturbidimetric protein determination, depends on the time of first measurement, on the subsequent measurement time interval and on the composition of the reaction medium. The IgG/anti-IgG system has been chosen as an example to demonstrate how the reaction parameters can be optimized. In this context particular consideration has been given to some of the analyzers commonly used in larger laboratories (ABA-100, CentrifiChem, Cobas Bio, GEMSAEC, Gilford 3500).