ABSTRACT
IBD (inflammatory bowel disease), where CD (Crohn's disease) and UC (ulcerative colitis) represent the two main forms, are chronic inflammatory conditions of the intestine. Macrophages play a central role in IBD pathogenesis and are regulated by major differentiation factors such as CSF-1 (colony-stimulating factor 1) in homoeostasis and inflammation. IL (interleukin)-34 has recently been discovered as a second ligand for CSF-1R (CSF-1 receptor). However, expression and involvement of IL-34 in IBD remain unknown. In the present paper, we investigated the expression of IL34, CSF1 and their shared receptor CSF1R in normal human ileum and colon, in inflamed and non-inflamed tissues of CD and UC patients, and in a mouse model of experimental colitis. We found distinct expression patterns of IL34 and CSF1 in ileum and colon, with higher IL34 in ileum and, in contrast, higher CSF1 in colon. Furthermore, IL34 and CSF1 expression was increased with inflammation in IBD patients and in experimental colitis. In humans, infiltrating cells of the lamina propria and intestinal epithelial cells expressed IL-34, and TNF-α (tumour necrosis factor α) regulated IL-34 expression in intestinal epithelial cells through the NF-κB (nuclear factor κB) pathway. These data demonstrate the expression pattern of IL-34 in ileum and colon and suggest IL-34 as a new modulator of inflammation in IBD.
Subject(s)
Colitis, Ulcerative/metabolism , Inflammatory Bowel Diseases/metabolism , Interleukins/metabolism , Animals , Colitis, Ulcerative/immunology , Humans , Inflammatory Bowel Diseases/immunology , Macrophages/metabolism , Mice , NF-kappa B/metabolism , Tumor Necrosis Factor-alpha/metabolismABSTRACT
The purpose of the present study was to evaluate the effects of maternal protein and energy restriction during lactation on the bodyweight and skull dimensions of pups at weaning. At parturition, Wistar rat dams were randomly assigned to the following groups: (i) control group (C), free access to a standard laboratory diet containing 23% protein; (ii) protein-energy-restricted group (PER), free access to an isoenergetic, protein-restricted diet containing 8% protein; and (iii) energy-restricted group (ER), restricted amounts of a standard laboratory diet. The dimensions of excised pup skulls were measured directly using pre-established anatomical points. Morphometrical analysis of the skulls showed that most of the measurements in the ER and PER groups were significantly lower than in the control group, with the greatest reductions occurring in the PER group. These results show that protein and energy restriction during lactation have an important influence on pup skull development.
Subject(s)
Animals, Newborn/growth & development , Lactation/physiology , Malnutrition/physiopathology , Maternal Nutritional Physiological Phenomena/physiology , Skull/growth & development , Animals , Animals, Newborn/physiology , Body Weight/physiology , Diet, Protein-Restricted/adverse effects , Energy Metabolism/physiology , Female , Male , Models, Animal , Rats , Rats, WistarABSTRACT
BACKGROUND: Long-chain polyunsaturated fatty acids omega-3 and omega-6 (LC-PUFA n-3 and n-6) can function as important inflammatory modulators and also have a strong effect in the proresolving inflammatory processes. The aim of the authors is to analyze the serum levels of docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA), docosapentaenoic acid (DPA), and arachidonic acid (AA) in patients with generalized chronic periodontitis (GCP) and compare these results with serum levels of patients with gingivitis only. METHODS: Twenty-one patients with untreated GCP (mean age: 46.0 ± 8.8 years) and 16 patients with gingivitis only (mean age: 31.5 ± 7.5 years) were investigated. The clinical examination included probing depth (PD), clinical attachment level, bleeding on probing, and plaque index. Blood samples were analyzed for the presence of DHA, EPA, DPA, and AA using gas chromatography. RESULTS: Significantly higher levels of DHA, DPA, EPA, and AA were observed in patients with GCP when compared with patients with gingivitis (P = 0.007, P = 0.004, P = 0.033, and P = 0.001, respectively). The differences were still significant even after the adjustments for age and sex. The PD showed a significant positive correlation with DHA (r = 0.5; P = 0.003), DPA (r = 0.6; P <0.001), and AA (r = 0.6; P <0.001). CONCLUSION: The present findings suggest that serum levels of LC-PUFA n-3 and n-6 may be affected by the severity of periodontal disease.