ABSTRACT
Course treatment with IFN-α2b immobilized on polyethylene glycol stimulates phagocytic activity of peritoneal macrophages and neutrophils, enhances humoral immune response in CBA/CaLac mice, stimulates IL-4 synthesis, and suppresses IFN-γ production by mitogenstimulated splenocytes from experimental animals.
Subject(s)
Adjuvants, Immunologic/administration & dosage , Drug Carriers/administration & dosage , Interferon-alpha/administration & dosage , Macrophages, Peritoneal/drug effects , Polyethylene Glycols/administration & dosage , Animals , Antigen Presentation/drug effects , Cells, Cultured , Drug Evaluation, Preclinical , Immunity, Cellular/drug effects , Immunity, Humoral/drug effects , Interferon alpha-2 , Macrophages, Peritoneal/immunology , Male , Mice, Inbred CBA , Neutrophils/drug effects , Neutrophils/immunology , Phagocytosis/drug effects , Spleen/drug effects , Staphylococcus aureus/immunologyABSTRACT
The effects of extracts from licorice (Glycyrrhisa glabra), great nettle (Urtica dioica), common burdock (Arctium lappa), and bur marigold (Bidens tripartite) on the humoral and cellular immune response and nonspecific resistance in mice were studied. Burdock and bur marigold extracts stimulated the humoral immune response, nettle and licorice extracts stimulated cellular response and nonspecific resistance, their effects being superior to those of pharmacopoeial Echinacea purpurea tincture.
Subject(s)
Immune System/drug effects , Immunity, Humoral/drug effects , Plant Extracts/pharmacology , Animals , Antibody-Producing Cells/drug effects , Antibody-Producing Cells/immunology , Antigens/immunology , Arctium/chemistry , Bidens/chemistry , Cell Count , Erythrocytes/immunology , Glycyrrhiza/chemistry , Male , Mice , Mice, Inbred CBA , Sheep , Spleen/cytology , Spleen/drug effects , Spleen/immunology , Urtica dioica/chemistryABSTRACT
Immune reactions of the progeny of female Wistar rats injected with paclitaxel a single MPD of 1 and 3 months before mating with intact males were studied. Thymic hyperplasia was detected in the progeny of female rats mated 1 month after treatment. Disorders in the antibody-specific and antibody-nonspecific mechanisms of the immune response were detected in the progeny of females mated with intact males 3 months after cytostatic treatment.
Subject(s)
Immunity/drug effects , Paclitaxel/toxicity , Animals , Animals, Newborn , Cytostatic Agents/toxicity , Female , Hyperplasia/chemically induced , Immunity/immunology , Male , Pregnancy , Prenatal Exposure Delayed Effects , Rats , Rats, Wistar , Thymus Gland/drug effects , Thymus Gland/pathology , Time FactorsABSTRACT
Quantitative composition and functional activity of immunocompetent cells differ in mice of different strains. The counts of T cells in the bone marrow and spleen, proliferative activity of T cells in the spleen, levels of IL-2 and IL-10 production by splenic T cells, number of antigen-specific T cells and their functional activity are low in C57Bl/6, BALB/c, and CC57W mice and high in CBA/CaLac, DBA/2, and C3H animals. Low phagocytic activity of peritoneal macrophages was detected in BALB/c and CC57W mice and high activity in C3H animals. The content of antibody-producing cells in the spleens of C57Bl/6, BALB/c, and CC57W mice is higher than in CBA/CaLac, DBA/2, C3H, A/SN, and AKR/JY mice. Functional activity of B cells is lower in BALB/c and CC57W compared to CBA/CaLac and DBA/2 mice.
Subject(s)
Immune System/physiology , Animals , CD4-Positive T-Lymphocytes/metabolism , CD8-Positive T-Lymphocytes/metabolism , Macrophages/metabolism , Macrophages, Peritoneal/metabolism , Mice , Mice, Inbred BALB C , Mice, Inbred C3H , Mice, Inbred C57BL , Mice, Inbred CBA , Mice, Inbred DBA , Phagocytosis , Species Specificity , Spleen/metabolismABSTRACT
Single injection of antineoplastic drugs cyclophosphamide and cisplatin (Platidiam) in maximum permissible doses to CBA/CaLac mice impaired morphological composition of hemopoietic and lymphoid organs (thymus and spleen). Drug-induced changes persisted for 6 months. Cyclophosphamide caused more severe disturbances in the lymphoid stem in the bone marrow, thymus, and spleen and more strongly stimulated erythropoiesis in the spleen compared to cisplatin. Immunization of mice with thymus-dependent antigen (sheep erythrocytes) after injection of cytostatics accelerated regenerative processes in hemopoietic and lymphoid organs. However, cell disorganization 3 and 6 months after immunization did not differ from that caused by antineoplastic drugs and was even more pronounced in the thymus by the 6th month of observations.
Subject(s)
Antigens/immunology , Antineoplastic Agents/pharmacology , Cisplatin/pharmacology , Cyclophosphamide/pharmacology , Hematopoietic Stem Cells/drug effects , Spleen/immunology , Thymus Gland/immunology , Animals , Erythrocytes/immunology , Hematopoietic Stem Cells/cytology , Hematopoietic Stem Cells/immunology , Humans , Immunization , Male , Mice , Mice, Inbred CBA , Spleen/cytology , Spleen/drug effects , Thymus Gland/cytology , Thymus Gland/drug effects , Time FactorsABSTRACT
Single intraperitoneal injection of cyclophosphamide in a maximum permissible dose (250 mg/kg) followed by immunization wih thymus-dependent antigen (sheep erythrocytes) markedly suppressed the formation of antibody-producing cells in the spleen, especially at early stages of cytostatic disease, and delayed the synthesis of specific antibodies in male CBA/CaLac mice. Platidiam (cisplatin) injected in comparable doses 4 days before immunization practically did not suppress the formation of antibody-producing cells and their functional activity, but being injected 30 days before immunization reduced the number of antibody-producing cells.
Subject(s)
Antibody Formation/drug effects , Antineoplastic Agents/adverse effects , Cyclophosphamide/adverse effects , Animals , Antineoplastic Agents/immunology , Cyclophosphamide/administration & dosage , Cyclophosphamide/immunology , Male , MiceABSTRACT
Mice of different strains can be classified by the intensity of humoral immune reaction to thymus-dependent antigen as high, moderate, and low responders. High and moderate responders (CBA/CaLac, DBA/1, BALB/c) are characterized by high sensitivity of the productive phase of humoral immune response and phagocytic activity of macrophages to immobilization stress. In low responders (C57BL/6, CC57W) stress only slightly affected the productive phase of the humoral immune response, but activity of peritoneal macrophages decreased. These differences in the reactions of the immune system of inbred mice after immobilization stress reflect different reactions of the immune system to extreme factors.
Subject(s)
Immobilization , Animals , Catecholamines/metabolism , Immune System , Macrophages/metabolism , Mice , Mice, Inbred BALB C , Mice, Inbred C57BL , Mice, Inbred CBA , Mice, Inbred DBA , Mice, Inbred Strains , Phagocytosis , Species Specificity , Stress, Physiological , Thymus Gland/pathology , Time FactorsABSTRACT
The responses of immunocompetent cells to thymus-dependent antigen differ in mice of different strains. Immunization stimulated phagocytic activity of peritoneal macrophages in CBA/CaLac, DBA/2, and BALB/c mice and suppressed it in CC57W mice. By the formation of antibody-producing cells in the spleen in response to thymus-dependent antigen DBA/2 and CBA/CaLac mice can be classified as high responders, BALB/c mice as medium-responders, and C57Bl/6 and CC57W mice as low responders.
Subject(s)
Mice, Inbred Strains/immunology , Thymus Gland/immunology , Animals , Antibody Formation , Antibody-Producing Cells/immunology , Genotype , Immunization , Macrophages, Peritoneal/immunology , Mice , Mice, Inbred C57BL/immunology , Mice, Inbred CBA/immunology , Mice, Inbred DBA/immunology , Species Specificity , Spleen/immunologyABSTRACT
T cells were most sensitive to cyclophosphamide in DBA/2 mice, while in C57Bl/6 mice both T and B cells were sensitive. The formation of antibody-producing cells and the production of specific antibodies were delayed in DBA/2 mice immunized after pretreatment with antitumor drug. Accumulation of antibody-producing cells in the spleen was more active in immunized C57Bl/6 mice treated with cyclophosphamide compared to animals not treated with cyclophosphamide.
Subject(s)
Antineoplastic Agents, Alkylating/pharmacology , B-Lymphocytes/drug effects , Cyclophosphamide/pharmacology , Macrophages/drug effects , T-Lymphocytes/drug effects , Animals , Antigen-Presenting Cells/drug effects , Bone Marrow Cells/drug effects , Bone Marrow Cells/immunology , Mice , Mice, Inbred C57BL , Mice, Inbred DBA , Spleen/cytology , Spleen/immunologyABSTRACT
We revealed differences in quantitative composition and functional activity of lymphoid cells in intact mice of different strains. Cellularity and counts of lymphoid elements in hemopoietic and lymphoid organs, proliferative activity of T and B lymphocytes, and counts of CD4+ and CD8+ lymphocytes in the spleen were minimum in CC57W and Balb/c mice and maximum in CBA/CaLac and DBA/2 mice. The highest absolute content of lymphoid elements in the spleen was detected in Balb/c mice, while CC57W mice had the highest content of these elements in the thymus.