ABSTRACT
Radially and azimuthally polarized beams in a single transverse mode are generated from a commercially available vertical cavity surface emitting laser (VCSEL) in an external cavity with a birefringent rutile lens, of which the c axis is parallel to the optical axis of the cavity, to select favorable polarization. Additionally, a vector Bessel-Gaussian beam is generated from a VCSEL, which is fabricated to oscillate with a linear polarization in a fixed direction in free running, in the same way. These results clearly show the potential ability of VCSELs to generate vector beams, which will be essential to space-division multiplexing in the future optical communication.
ABSTRACT
Microfluidic systems have significant implications in the field of cell separation since they could provide platforms with inexpensive, disposable and sterile structures. Here, we present a novel strategy to integrate microfluidic sorters into a single chip for high throughput sorting. Our parallel sorter consists of a microfluidic chip with a three-dimensional channel network that utilizes flow switching by a heat-induced sol-gel transition of thermoreversible gelation polymer. The 8 parallel sheathed sample flows were realized by injecting sample and buffer solutions into only 2 inlets. The sheathed flows enabled disposal of unwanted sample waste without laser irradiation, and collection of wanted sample upon irradiation. As an application of the sorter, two kinds of fluorescent microspheres were separated with recovery ratio and purity of 70% or 90% at throughputs of about 100 or 20 particles per second, respectively. Next, Escherichia coli cells expressing green fluorescent protein were separated from those expressing DsRed with recovery ratio and purity of 90% at a throughput of about 20 cells per second.