ABSTRACT
Herbicide use, mainly glyphosate, has been intense in worldwide agriculture, including in the Brazilian Amazon region. This study aimed to validate a method for determining glyphosate and its degradation product, AMPA, and glufosinate by HPLC-FL in 58 water samples collected at the Santarém plateau region (Planalto Santareno), in the western of Pará state, Brazil. The method involves filtration and direct injection in the HPLC-FL for AMPA analysis, or previous concentration (10×) by lyophilization for glufosinate and glyphosate analysis. Analytes were oxidized and complexed with o-phthalaldehyde and 2-mercaptoethanol in a post-column reaction before fluorescence detection. LOQs for AMPA, glyphosate and glufosinate were established at 0.5, 0.2 and 0.3 µg L-1, respectively. A total of 58 samples were collected. Glyphosate and glufosinate were not detected in any of the 30 surface water samples collected in 2015 (Subject(s)
Aminobutyrates/analysis
, Chromatography, High Pressure Liquid/methods
, Glycine/analogs & derivatives
, Water Pollutants, Chemical/analysis
, Water/chemistry
, alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid/analysis
, Brazil
, Environmental Monitoring
, Fluorescence
, Freeze Drying
, Glycine/analysis
, Herbicides/analysis
, Limit of Detection
, Reproducibility of Results
, Glyphosate