ABSTRACT
The embryonic mouse cortex displays a striking low caudo-medial and high rostro-lateral graded expression of the homeoprotein transcription factor Pax6, which presents both cell autonomous and direct noncell autonomous activities. Through the genetic induction of anti-Pax6 single-chain antibody secretion, we have analyzed Pax6 noncell autonomous activity on the migration of cortical hem- and septum-derived Cajal-Retzius (CR) neurons by live imaging of flat mount developing cerebral cortices. Blocking extracellular Pax6 disrupts tangential CR cell migration patterns by decreasing the distance traveled and changing both directionality and depth at which CR cells migrate. Tracking of single CR cells in mutant cortices revealed that extracellular Pax6 neutralization enhances contact repulsion in medial regions yet reduces it in lateral regions. This study demonstrates that secreted Pax6 controls neuronal migration and distribution and suggests that it acts as a bona fide morphogen at an early stage of cerebral cortex development.
Subject(s)
Cell Movement , Neocortex/growth & development , Neurons/physiology , PAX6 Transcription Factor/physiology , Animals , Mice, Inbred C57BL , Mice, TransgenicABSTRACT
Accumulation of non-cell autonomous Otx2 homeoprotein in postnatal mouse visual cortex (V1) has been implicated in both the onset and closure of critical period (CP) plasticity. Here, we show that a genetic point mutation in the glycosaminoglycan recognition motif of Otx2 broadly delays the maturation of pivotal parvalbumin-positive (PV+) interneurons not only in V1 but also in the primary auditory (A1) and medial prefrontal cortex (mPFC). Consequently, not only visual, but also auditory plasticity is delayed, including the experience-dependent expansion of tonotopic maps in A1 and the acquisition of acoustic preferences in mPFC, which mitigates anxious behavior. In addition, Otx2 mis-localization leads to dynamic turnover of selected perineuronal net (PNN) components well beyond the normal CP in V1 and mPFC. These findings reveal widespread actions of Otx2 signaling in the postnatal cortex controlling the maturational trajectory across modalities. Disrupted PV+ network function and deficits in PNN integrity are implicated in a variety of psychiatric illnesses, suggesting a potential global role for Otx2 function in establishing mental health.
Subject(s)
Auditory Cortex/physiology , Neuronal Plasticity/genetics , Otx Transcription Factors/genetics , Prefrontal Cortex/physiology , Animals , Auditory Cortex/metabolism , Cell Line , Extracellular Matrix/metabolism , Gene Knock-In Techniques , Glycosaminoglycans/metabolism , Interneurons/physiology , Mice , Mice, Inbred Strains , Neuronal Plasticity/physiology , Otx Transcription Factors/metabolism , Parvalbumins/metabolism , Point Mutation , Prefrontal Cortex/metabolism , Protein Domains , Visual Cortex/metabolismABSTRACT
The concept of homeoprotein transduction as a novel signaling pathway has dramatically evolved since it was first proposed in 1991. It is now well established in several biological systems from plants to mammals. In this review, the different steps that have led to this unexpected observation are recalled and the developmental and physiological models that have allowed us (and a few others) to consolidate the original hypothesis are described. Because homeoprotein signaling is active in plants and animals it is proposed that it has predated the separation between animals and plants and is thus very ancient. This may explain why the basic phenomenon of homeoprotein transduction is so minimalist, requiring no specific receptors or transduction pathways beside those offered by mitochondria, organelles present in all eukaryotic cells. Indeed complexity has been added in the course of evolution and the conservation of homeoprotein transduction is discussed in the context of its synergy with bona fide signaling mechanism that may have added robustness to this primitive cell communication device. The same synergy possibly explains why homeoprotein signaling is important both in embryonic development and in adult functions fulfilled by signaling entities (e.g. growth factors) themselves active throughout development and in the adult. The cell biological mechanism of homeoprotein transfer is also discussed. Although it is clear that many questions are still in want of precise answers, it appears that the sequences responsible both for secretion and internalization are in the DNA-binding domain and very highly conserved among most homeoproteins. On this basis, it is proposed that this signaling pathway is likely to imply as many as 200 proteins that participate in a myriad of developmental and physiological pathways.
ABSTRACT
Some proteins are internalized by live cells by a process that does not involve classical endocytosis and thus gain direct access to the cytoplasm and nucleus. These same proteins are often secreted, despite the absence of a signal peptide. Recent studies of this unexpected mode of intercellular signaling have opened the way for biotechnological developments.
Subject(s)
Gene Products, tat/metabolism , Homeodomain Proteins/metabolism , Amino Acid Sequence , Humans , Molecular Sequence Data , Signal TransductionABSTRACT
Internalization of exogenous macromolecules by live cells provides a powerful approach for studying cellular functions. Understanding the mechanism of transfer from the extracellular milieu to the cytoplasm and nucleus could also contribute to the development of new therapeutic approaches. This article summarizes the unexpected properties of penetratins, a class of peptides with translocating properties and capable of carrying hydrophilic compounds across the plasma membrane. This unique system allows direct targeting of oligopeptides and oligonucleotides to the cytoplasm and nucleus, is non-cell-type specific and highly efficient, and therefore has several applications of potential cell-biology and clinical interest.
Subject(s)
Cell Nucleus/metabolism , Cytoplasm/metabolism , Homeodomain Proteins/chemistry , Homeodomain Proteins/metabolism , Amino Acid Sequence , Animals , Biological Transport/physiology , Homeodomain Proteins/genetics , Molecular Sequence DataABSTRACT
The influence of J1/tenascin adsorbed to polyornithine-conditioned plastic (substrate-bound J1/tenascin) and J1/tenascin present in the culture medium (soluble J1/tenascin) on neurite outgrowth was studied with cultured single cells from hippocampus and mesencephalon of embryonic rats. Neurons at low density grew well on J1/tenascin substrates and extended neurites that were approximately 40% longer than on the polyornithine control substrate after 24 h in vitro. The neurite outgrowth promoting effect of substrate bound J1/tenascin was largely abolished in the presence of mAb J1/tn2, but not by mAb J1/tn1. In contrast to the neurite growth-promoting effects of substrate bound J1/tenascin, neurite outgrowth on polyornithine, laminin, fibronectin, or J1/tenascin as substrates was inhibited by addition of soluble J1/tenascin to the cultures. Neither of the two mAbs neutralized the neurite outgrowth-inhibitory properties of soluble J1/tenascin. In contrast to their opposite effects on neurite outgrowth, both substrate-bound and soluble J1/tenascin reduced spreading of the neuronal cell bodies, suggesting that the neurite outgrowth-promoting and antispreading effects are mediated by two different sites on the molecule. This was further supported by the inability of the mAb J1/tn2 to neutralize the antispreading effect. The J1/tn2 epitope localizes to a fibronectin type III homology domain that is presumably distinct from the putative Tn68 cell-binding domain of chicken tenascin for fibroblasts, as shown by electronmicroscopic localization of antibody binding sites. We infer from these experiments that J1/tenascin contains a neurite outgrowth promoting domain that is distinguishable from the cell-binding site and presumably not involved in the inhibition of neurite outgrowth or cell spreading. Our observations support the notion that J1/tenascin is a multifunctional extracellular matrix molecule.
Subject(s)
Axons/physiology , Cell Adhesion Molecules, Neuronal/pharmacology , Extracellular Matrix Proteins/pharmacology , Hippocampus/physiology , Mesencephalon/physiology , Neurons/physiology , Animals , Antibodies, Monoclonal , Axons/drug effects , Axons/ultrastructure , Binding Sites, Antibody , Cell Adhesion/drug effects , Cells, Cultured , Embryo, Mammalian , Epitopes/analysis , Extracellular Matrix/physiology , Hippocampus/ultrastructure , Kinetics , Mesencephalon/ultrastructure , Microscopy, Electron , Neurons/drug effects , Neurons/ultrastructure , Rats , TenascinABSTRACT
The amyloid precursor protein (APP) is a transmembrane protein expressed in several cell types. In the nervous system, APP is expressed by glial and neuronal cells, and several lines of evidence suggest that it plays a role in normal and pathological phenomena. To address the question of the actual function of APP in normal developing neurons, we undertook a study aimed at blocking APP expression using antisense oligonucleotides. Oligonucleotide internalization was achieved by linking them to a vector peptide that translocates through biological membranes. This original technique, which is very efficient and gives direct access to the cell cytosol and nucleus, allowed us to work with extracellular oligonucleotide concentrations between 40 and 200 nM. Internalization of antisense oligonucleotides overlapping the origin of translation resulted in a marked but transient decrease in APP neosynthesis that was not observed with the vector peptide alone, or with sense oligonucleotides. Although transient, the decrease in APP neosynthesis was sufficient to provoke a distinct decrease in axon and dendrite outgrowth by embryonic cortical neurons developing in vitro. The latter decrease was not accompanied by changes in the spreading of the cell bodies. A single exposure to coupled antisense oligonucleotides at the onset of the culture was sufficient to produce significant morphological effects 6, 18, and 24 h later, but by 42 h, there were no remaining significant morphologic changes. This report thus demonstrates that amyloid precursor protein plays an important function in the morphological differentiation of cortical neurons in primary culture.
Subject(s)
Amyloid/genetics , Cerebral Cortex/physiology , Down-Regulation , Neurites/physiology , Protein Precursors/genetics , Amyloid/isolation & purification , Animals , Base Sequence , Cells, Cultured , Dose-Response Relationship, Drug , Immunohistochemistry , Molecular Sequence Data , Neurites/drug effects , Oligonucleotides, Antisense/metabolism , Oligonucleotides, Antisense/pharmacology , Prions , Protein Precursors/isolation & purification , RatsABSTRACT
Spinal motoneuron development is regulated by a variety of intrinsic and extrinsic factors. Among these, a possible role for homeoproteins is suggested by their expression in the motoneuron at relatively late stages. To investigate their possible involvement in motoneuron growth, we adapted a novel technique recently developed in this laboratory, based on the ability of the 60 amino acid-long homeobox of Antennapedia (pAntp) to translocate through the neuronal membrane and to accumulate in the nucleus (Joliot, A. H., C. Pernelle, H. Deagostini-Bazin, and A. Prochiantz. 1991. Proc. Natl. Acad. Sci. USA. 88:1864-1868; Joliot, A. H., A. Triller, M. Volovitch, C. Pernelle, and A. Prochiantz. 1991. New Biol. 3:1121-1134). Motoneurons from E5 chicken spinal cord were incubated with pAntp, purified by panning on SC1 antibody and plated on polyornithine/laminin substrata without further addition of pAntp. After 24 h, neurite outgrowth was already extensive in controls. In cultures of motoneurons that had been preincubated with 10(-7) M pAntp, neurite length was doubled; a similar effect was obtained using postnatal muscle extracts. Morphological analysis using a neurofilament marker specific for axons indicated that the homeobox peptide enhances primarily axonal elongation and branching. To test the hypothesis that the biological activity of pAntp involves its specific attachment to cognate homeobox binding sites present in the genome, we generated a mutant of pAntp called pAntp40P2, that was still able to translocate through the motoneuron membrane and to reach the nucleus, but had lost the specific DNA-binding properties of the wild-type peptide. Preincubation of pAntp40P2 with purified motoneurons failed to increase neurite outgrowth. This finding raises the possibility that motoneuron growth is controlled by homeobox proteins.
Subject(s)
Cell Differentiation/drug effects , Cell Division/drug effects , DNA-Binding Proteins/pharmacology , Homeodomain Proteins , Motor Neurons/cytology , Neurites/ultrastructure , Nuclear Proteins , Spinal Cord/cytology , Transcription Factors , Animals , Antennapedia Homeodomain Protein , Axons/drug effects , Axons/ultrastructure , Cell Survival/drug effects , Cells, Cultured , Chick Embryo , DNA-Binding Proteins/genetics , Motor Neurons/drug effects , Mutagenesis, Site-Directed , Neurites/drug effects , Recombinant Proteins/pharmacology , Tubulin/analysisABSTRACT
The rab genes code for small GTP binding proteins that share with p21ras the ability to bind and hydrolyze GTP. They present significant sequence homologies with the products of YPT1 and SEC4, two small GTP binding proteins involved in the regulation of secretion in the yeast. Several rab genes are expressed in the developing and adult mouse brain. To test directly the possible involvement of these genes in neuronal differentiation, purified rab proteins produced in E. coli were introduced into neurons dissociated from E15 rat midbrain. The most striking effects were obtained with rab2 protein (rab2p). Compared with untreated cells, neurons loaded with rab2p presented an enhanced adhesion to the culture substratum. This phenomenon was visible 3 hr after seeding and was followed within 24 hr by a dramatic increase in neurite growth. Loading the same population of neurons with the products of four other rab genes either decreased neuronal adhesion and neurite growth or had no effect. These experiments suggest that the expression of rab2p plays an important role in neuronal differentiation.
Subject(s)
Cell Adhesion Molecules, Neuronal/pharmacology , GTP-Binding Proteins/pharmacology , Neurons/cytology , Animals , Cell Adhesion/drug effects , Cell Adhesion Molecules, Neuronal/genetics , Cell Adhesion Molecules, Neuronal/physiology , Cell Differentiation/drug effects , Cells, Cultured , GTP-Binding Proteins/genetics , GTP-Binding Proteins/physiology , Mesencephalon/cytology , Mesencephalon/drug effects , Mesencephalon/metabolism , Neurons/drug effects , Neurons/physiology , RatsABSTRACT
It was previously shown that newly formed oligodendrocytes depend on axons for their survival, but the nature of the axon-derived survival signal(s) remained unknown. We show here that neuregulin (NRG) supports the survival of purified oligodendrocytes and aged oligodendrocyte precursor cells (OPCs) but not of young OPCs. We demonstrate that axons promote the survival of purified oligodendrocytes and that this effect is inhibited if NRG is neutralized. In the developing rat optic nerve, we provide evidence that delivery of NRG decreases both normal oligodendrocyte death and the extra oligodendrocyte death induced by nerve transection, whereas neutralization of endogenous NRG increases the normal death. These results suggest that NRG is an axon-associated survival signal for developing oligodendrocytes.
Subject(s)
Axons/metabolism , Cell Survival/physiology , Neuregulin-1/metabolism , Oligodendroglia/metabolism , Optic Nerve/metabolism , Animals , Axotomy , COS Cells , Cell Count , Cell Survival/drug effects , Cells, Cultured , Cellular Senescence/drug effects , Coculture Techniques , Dose-Response Relationship, Drug , Ganglia, Spinal/cytology , Ganglia, Spinal/drug effects , Ganglia, Spinal/metabolism , Neuregulin-1/genetics , Neuregulin-1/pharmacology , Oligodendroglia/cytology , Oligodendroglia/drug effects , Optic Nerve/cytology , Optic Nerve/drug effects , Optic Nerve/growth & development , Rats , Rats, Sprague-Dawley , Signal Transduction/drug effects , Stem Cells/cytology , Stem Cells/drug effects , TransfectionABSTRACT
BACKGROUND: Engrailed-1 and Engrailed-2 are homeoproteins--transcription factors implicated in the morphogenesis of discrete structures. Engrailed proteins have a role in patterning the midbrain-hindbrain region and are expressed in the nuclei of rat embryo midbrain-hindbrain cells. We have previously found that both endogenous and exogenously expressed Engrailed proteins also associate with membrane regions implicated in signal transduction and secretion. Within total membrane fractions, a small proportion of Engrailed--about 5%--is protected against proteinase K proteolysis, suggesting that Engrailed has access to a luminal compartment. Together with our finding that homeodomains and homeoproteins can be internalized by live cells, these observations suggest that Engrailed might act as a polypeptidic messenger. In order to investigate this possibility, we looked to see if Engrailed could be secreted. RESULTS: Engrailed expressed in COS cells can be recovered in abutting primary neurons and this is dependent on a short sequence in its homeodomain distinct from 'classical' secretion signals. This sequence, which overlaps with the sequence necessary for Engrailed internalization and which is highly conserved among homeoproteins, is the first example of an 'unconventional' sequence necessary for secretion. Less than 50% of total intracellular Engrailed is secreted and there is a correlation between secretion and access to the membrane compartment where the protein is protected against proteinase K. CONCLUSIONS: Our results lend weight to the proposal that Engrailed, and possibly other homeoproteins, might act as intercellular polypeptidic messengers.
Subject(s)
Homeodomain Proteins/metabolism , Nerve Tissue Proteins/metabolism , Neurons/metabolism , Protein Sorting Signals/metabolism , Animals , Binding Sites , COS Cells , Cells, Cultured , Coculture Techniques , Endopeptidase K/metabolism , RatsABSTRACT
BACKGROUND: Activated receptor tyrosine kinases bind downstream effector molecules with high affinity. Provided that they can be introduced into cells, peptides corresponding to these high-affinity sites should be able to compete for the interaction and thereby inhibit specific signal transduction cascades. The high-affinity binding site for phospholipase C gamma (PLCgamma) on the activated fibroblast growth factor receptor (FGFR) is centred around the tyrosine at position 766 (766Tyr), and peptides corresponding to this site inhibit PLCgamma binding to the receptor in vitro. A 16 amino-acid peptide from the third helix of the Antennapedia homeodomain protein has recently been shown to be able to act as an internalization vector that can deliver other peptides into cells. Here, we have designed a peptide that contains both the internalization sequence and the FGFR high-affinity binding site for PLCgamma, and tested it in cultures of cerebellar neurons for its ability to inhibit the activation of PLCgamma by basic FGF. RESULTS: The peptide containing the FGFR high-affinity binding site for PLCgamma inhibited phospholipid hydrolysis stimulated by basic FGF with a maximal effect at 1 microg ml-1. Phosphorylation of 766Tyr was required for this effect. The phosphorylated peptide had no effect on phospholipid hydrolysis stimulated by platelet-derived growth factor, neurotrophin-3 and bradykinin. The phosphorylated peptide also inhibited neurite outgrowth stimulated by FGF, but had no effect on neurite outgrowth stimulated by agents that activate the FGFR signal transduction cascade downstream from the activation of PLCgamma. CONCLUSIONS: Cell-permeable peptides can be designed that inhibit the function of receptor tyrosine kinases. In this context we have developed a peptide that prevents the FGFR from activating PLCgamma, and have used this peptide to obtain the first direct evidence that activation of PLCgamma is required for the neurite outgrowth response stimulated by basic FGF.
Subject(s)
Fibroblast Growth Factor 2/pharmacology , Neurites , Phosphatidylinositols/metabolism , Phosphopeptides/pharmacology , Amino Acid Sequence , Animals , Binding Sites , Cell Membrane Permeability , Cells, Cultured , Fibroblast Growth Factor 2/antagonists & inhibitors , Hydrolysis , Isoenzymes/metabolism , Molecular Sequence Data , Phospholipase C gamma , Rats , Receptors, Fibroblast Growth Factor/metabolism , Type C Phospholipases/metabolismABSTRACT
An important issue in developmental biology is the identification of homeoprotein target genes. We have developed a strategy based on the internalization and nuclear addressing of exogenous homeodomains, using an engrailed homeodomain (EnHD) to screen an embryonic stem (ES) cell gene trap library. Eight integrated gene trap loci responded to EnHD. One is within the bullous pemphigoid antigen 1 (BPAG1) locus, in a region that interrupts two neural isoforms. By combining in vivo electroporation with organotypic cultures, we show that an already identified BPAG1 enhancer/promoter is differentially regulated by homeoproteins Hoxc-8 and Engrailed in the embryonic spinal cord and mesencephalon. This strategy can therefore be used for identifying and mutating homeoprotein targets. Because homeodomain third helices can internalize proteins, peptides, phosphopeptides, and antisense oligonucleotides, this strategy should be applicable to other intracellular targets for characterizing genetic networks involved in a large number of physiopathological states.
Subject(s)
Carrier Proteins , Cytoskeletal Proteins , Homeodomain Proteins/genetics , Nerve Tissue Proteins , Non-Fibrillar Collagens , Sequence Analysis, DNA/methods , Transcription Factors , Animals , Autoantigens/biosynthesis , Autoantigens/genetics , Brain/embryology , Brain/metabolism , Cell Nucleus/metabolism , Collagen/biosynthesis , Collagen/genetics , Cytoplasm/metabolism , Dystonin , Electroporation , Embryo, Mammalian/cytology , Homeodomain Proteins/biosynthesis , Homeodomain Proteins/chemistry , Homeodomain Proteins/metabolism , Mice , Models, Genetic , Plasmids/metabolism , Promoter Regions, Genetic , Protein Isoforms , RNA, Messenger/metabolism , Reverse Transcriptase Polymerase Chain Reaction , Spinal Cord/embryology , Spinal Cord/metabolism , Stem Cells/cytology , Collagen Type XVIIABSTRACT
The homeodomain of Antennapedia, a Drosophila transcription factor, translocates across biological membranes. Within this 60-amino-acid polypeptide, a shorter sequence of 16 amino acids was identified that can be used as an internalization vector for several types of cargo (i.e. other peptides and oligonucleotides) into the cytoplasm and nucleus of all cell types. This article describes our present understanding of this phenomenon and discusses its potential applications in cell biology.
Subject(s)
Homeodomain Proteins/metabolism , Nuclear Proteins , Transcription Factors/metabolism , Amino Acid Sequence , Animals , Antennapedia Homeodomain Protein , Cell Nucleus/metabolism , Conserved Sequence , Drosophila , Drosophila Proteins , Homeodomain Proteins/chemistry , Humans , Models, Structural , Molecular Sequence Data , Mutagenesis, Site-Directed , Point Mutation , Protein Structure, Secondary , Recombinant Proteins/chemistry , Recombinant Proteins/metabolism , Transcription, GeneticABSTRACT
When overexpressed, a short cytoplasmic domain of the amyloid precursor protein (APP), normally unmasked in the brain of Alzheimer's disease patients, activates caspase-3 and induces neuronal death. Death induction by this "Jcasp" domain is lost when tyrosine 653 is changed into an aspartate, suggesting specific interactions with unknown partners. To identify these putative partners and start to elucidate the mechanisms involved in Jcasp-induced cell death, we internalized a biotinylated version of the peptide into primary neurons and analyzed intracellular interacting proteins by pull-down and mass spectrometry. We find that SET protein, also called template-activating factor (TAF1beta) or phosphatase 2A inhibitor 2 (I2(PP2A)), specifically binds Jcasp early after internalization and that SET and Jcasp interact directly in vitro. Down-regulation of SET reduces Jcasp-induced cell death, confirming a role of this protein in Jcasp-induced apoptosis. Conversely, SET gain of function increases cell death, which suggests that SET level is crucial for neuronal survival/death. Taken together, these results suggest that SET is part of a neuronal apoptotic pathway related to Alzheimer's disease and provides a new entry in the analysis of this pathology.
Subject(s)
Amyloid beta-Protein Precursor/chemistry , Apoptosis , Chromosomal Proteins, Non-Histone/physiology , Cytoplasm/metabolism , Neurons/pathology , Transcription Factors/physiology , Alzheimer Disease/metabolism , Animals , Aspartic Acid/chemistry , Biological Assay , Brain/embryology , Cell Death , Cell Line, Tumor , Cell Survival , DNA-Binding Proteins , Down-Regulation , Histone Chaperones , Humans , Immunohistochemistry , Mass Spectrometry , Models, Biological , Neurons/metabolism , Oligonucleotides, Antisense/chemistry , Oligonucleotides, Antisense/pharmacology , Peptides/chemistry , Protein Binding , Protein Structure, Tertiary , Rats , Recombinant Proteins/chemistry , Silver Staining , Tyrosine/chemistryABSTRACT
Homeoproteins are well known for their role in defining the shape of organs during early development. The late expression of some homeogenes in the nervous system suggests that they might have other, additional functions, possibly in neurite growth and target recognition. The 60 amino acid-long peptide corresponding to the homeobox of Antennapedia (pAntp) translocates through the membrane of neurons in culture and reaches their nuclei. This process is followed by an enhanced morphological differentiation of the neurons. Internalization by neurons is four-fold that observed with fibroplasts. This difference is abolished upon treatment with Endo-N which specifically cleaves alpha,2-8 bonds in polysialic acid. To understand the mode of action of the peptide, we constructed three mutants modified in their capacity to specifically bind promoters and/or to translocate through the cell membrane. The biological properties of the mutants demonstrate that the neurotrophic action of pAntp requires its internalization and integrity of its specific DNA-binding capacity.
Subject(s)
DNA-Binding Proteins/physiology , Genes, Homeobox , Homeodomain Proteins , Neuropeptides/physiology , Nuclear Proteins/physiology , Transcription Factors , Animals , Antennapedia Homeodomain Protein , Cells, Cultured , RatsABSTRACT
To identify genes regulated by homeoprotein transcription factors in postnatal neurons, the DNA-binding domain (homeodomain) of Engrailed homeoprotein was internalized into rat cerebellum neurons. The internalized homeodomain (EnHD) acts as a competitive inhibitor of Engrailed and of several homeoproteins (Mainguy et al., 2000). Analysis by differential display revealed that microtubule-associated protein 1B (MAP1B) mRNA is upregulated by EnHD. This upregulation does not require protein synthesis, suggesting a direct effect of the homeodomain on MAP1B transcription. The promoter region of MAP1B was cut into several subdomains, and each subdomain was tested for its ability to bind Engrailed and EnHD and to associate with Engrailed-containing cerebellum nuclear extracts. In addition, the activity, and regulation by Engrailed, of each subdomain and of the entire promoter were evaluated in vivo by electroporation in the chick embryo neural tube. These experiments demonstrate that MAP1B promoter is regulated by Engrailed in vivo. Moreover, they show that one promoter domain that contains all ATTA homeoprotein cognate binding sites common to the rat and human genes is an essential element of this regulation. It is thus proposed that MAP1B, a cytoskeleton protein involved in neuronal growth and regeneration, is under homeoprotein transcriptional regulation.
Subject(s)
Gene Expression Regulation, Developmental , Homeodomain Proteins/metabolism , Microtubule-Associated Proteins/metabolism , Neurons/metabolism , Transcription Factors/metabolism , Animals , Binding Sites/physiology , Cells, Cultured , Central Nervous System/embryology , Central Nervous System/metabolism , Cerebellum/cytology , Cerebellum/embryology , Cerebellum/metabolism , Chick Embryo , Gene Expression Profiling , Genes, Reporter , Homeodomain Proteins/antagonists & inhibitors , Homeodomain Proteins/genetics , Mice , Microtubule-Associated Proteins/genetics , Neurons/cytology , Promoter Regions, Genetic/physiology , Protein Structure, Tertiary/physiology , RNA, Messenger/metabolism , Rats , Up-RegulationABSTRACT
Outgrowth of the dendrites and the axon is the basis of the establishment of the neuronal shape, and it requires addition of new membrane to both growing processes. It is not yet clear whether one or two exocytotic pathways are responsible for the respective outgrowth of axons and dendrites. We have previously shown that tetanus neurotoxin-insensitive vesicle-associated membrane protein (TI-VAMP) defines a novel network of tubulovesicular structures present both at the leading edge of elongating dendrites and axons of immature hippocampal neurons developing in primary culture and that TI-VAMP is an essential protein for neurite outgrowth in PC12 cells. Here we show that the expression of the N-terminal domain of TI-VAMP inhibits the outgrowth of both dendrites and axons in neurons in primary culture. This effect is more prominent at the earliest stages of the development of neurons in vitro. Expression of the N-terminal domain deleted form of TI-VAMP has the opposite effect. This constitutively active form of TI-VAMP localizes as the endogenous protein, particularly concentrating at the leading edge of growing axons. Our results suggest that a common exocytotic mechanism that relies on TI-VAMP mediates both axonal and dendritic outgrowth in developing neurons.
Subject(s)
Axons/physiology , Dendrites/physiology , Exocytosis/physiology , Neurons/metabolism , Animals , Autoantigens , Brain/cytology , Brain/metabolism , Calcium-Binding Proteins/metabolism , Calreticulin , Cells, Cultured , Electroporation , Endocytosis/physiology , Gene Expression , Green Fluorescent Proteins , In Vitro Techniques , Luminescent Proteins/genetics , Membrane Proteins/genetics , Membrane Proteins/metabolism , Mice , Neurons/cytology , Protein Isoforms/genetics , Protein Isoforms/metabolism , Protein Structure, Tertiary/physiology , Qa-SNARE Proteins , R-SNARE Proteins , Rats , Recombinant Fusion Proteins/genetics , Recombinant Fusion Proteins/metabolism , Ribonucleoproteins/metabolism , TransfectionABSTRACT
The established view in cellular biology dictates that the cellular internalization of hydrophilic macromolecules can only be achieved through the classical endocytosis pathway. However, in the past five years several peptides have been demonstrated to translocate across the plasma membrane of eukaryotic cells by a seemingly energy-independent pathway. These peptides have been used successfully for the intracellular delivery of macromolecules with molecular weights several times greater than their own. Cellular delivery using these cell-penetrating peptides offers several advantages over conventional techniques because it is efficient for a range of cell types, can be applied to cells en masse and has a potential therapeutic application.