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1.
Nutr Cancer ; 69(3): 505-511, 2017 Apr.
Article in English | MEDLINE | ID: mdl-28287317

ABSTRACT

Cancer cell resistance to chemotherapy is one of the obstacles for better cancer treatment, and inflammatory signaling pathways, such as NF-κB signaling pathway, have been recognized to be involved in such chemoresistance. In this study, we aim to identify a new approach for overcoming cancer chemoresistance by using natural compounds. As a result of screening by using Murine B16F10 melanoma cell line constitutively expressing NF-κB luciferase reporter gene, we identified Saikosaponin B2 as an effective inhibitor for etoposide-induced NF-κB activation in B16F10NFkB cells. Saikosaponin B2 sensitized etoposide-induced cell death in B16F10 melanoma cells through the induction of apoptosis. Along with apoptosis induction, we observed an induction of γ-H2AX expression, which is a molecular signature for DNA damage, upon the combination treatment of etoposide and Saikosaponin B2. Among Saikosaponin family compounds, we found that Saikosaponin B1, but not Saikosaponin A, sensitized etoposide-induced cytotoxicity implicating the structural requirement of Saikosaponin B for such chemosensitization. By testing the combination of Saikosaponin B1 and B2 with 9 clinical anticancer drugs, Saikosaponin B showed a certain preference in the combination with those tested anticancer drugs. Collectively, we conclude Saikosaponin B can be an attractive adjuvant for enhancing the clinical effect of cancer chemotherapy.


Subject(s)
Melanoma/drug therapy , Oleanolic Acid/analogs & derivatives , Saponins/pharmacology , Animals , Antineoplastic Agents/pharmacology , Apoptosis/drug effects , Cell Death/drug effects , Cell Line, Tumor , Cell Survival , Drug Resistance, Neoplasm , Etoposide/toxicity , Mice , NF-kappa B/genetics , NF-kappa B/metabolism , Oleanolic Acid/pharmacology , Signal Transduction
2.
Bioorg Med Chem Lett ; 27(9): 1967-1971, 2017 05 01.
Article in English | MEDLINE | ID: mdl-28342587

ABSTRACT

From the chloroform extract of the leaves of Uvaria dac, four new highly-oxygenated cyclohexene derivatives named uvaridacols I-L (1-4) were isolated together with nine previously reported compounds (5-13). Their structures were determined based on the extensive NMR spectroscopic data and circular dichroism spectroscopic analysis. Among the new compounds, uvaridacol L (4) displayed strong preferential cytotoxicity in the nutrient deprived medium against five different tested pancreatic cancer cell lines, PANC-1 (PC50, 20.1µM), PSN-1 (PC50, 9.7µM), MIA PaCa-2 (PC50, 29.1µM), Capan-1 (73.0µM) and KLM-1 (25.9µM).


Subject(s)
Antineoplastic Agents, Phytogenic/chemistry , Antineoplastic Agents, Phytogenic/pharmacology , Cyclohexenes/chemistry , Cyclohexenes/pharmacology , Oxygen/chemistry , Uvaria/chemistry , Antineoplastic Agents, Phytogenic/isolation & purification , Cell Line, Tumor , Cyclohexenes/isolation & purification , Drug Screening Assays, Antitumor , Humans , Pancreas/drug effects , Pancreatic Neoplasms/drug therapy , Plant Leaves/chemistry
3.
Cancer Sci ; 107(10): 1416-1421, 2016 Oct.
Article in English | MEDLINE | ID: mdl-27403934

ABSTRACT

Although the secretory matricellular protein connective tissue growth factor (CTGF) has been reported to be related to lung cancer metastasis, the precise mechanism by which CTGF regulates lung cancer metastasis has not been elucidated. In the present study, we show the molecular link between CTGF secretion and the p38 pathway in the invasive and metastatic potential of non-small-cell lung cancer (NSCLC). Among three different human NSCLC cell lines (PC-14, A549, and PC-9), their in vitro invasiveness was inversely correlated with the level of CTGF secretion. By supplementing or reducing CTGF secretion in NSCLC culture, dysregulation of the invasive and metastatic potential of NSCLC cell lines was largely compensated. By focusing on the protein kinases that are known to be regulated by CTGF, we found that the p38 pathway is a key downstream signal of CTGF to regulate the metastatic potential of NSCLC. Importantly, a negative correlation between CTGF and phosphorylation status of p38 was identified in The Cancer Genome Atlas lung adenocarcinoma dataset. In the context of the clinical importance of our findings, we showed that p38 inhibitor, SB203580, reduced the metastatic potential of NSCLC secreting low levels of CTGF. Collectively, our present findings indicate that the CTGF/p38 axis is a novel therapeutic target of NSCLC metastasis, particularly NSCLC secreting low levels of CTGF.


Subject(s)
Carcinoma, Non-Small-Cell Lung/metabolism , Carcinoma, Non-Small-Cell Lung/pathology , Connective Tissue Growth Factor/metabolism , Lung Neoplasms/metabolism , Lung Neoplasms/pathology , MAP Kinase Signaling System , p38 Mitogen-Activated Protein Kinases/metabolism , Animals , Cell Line, Tumor , Disease Models, Animal , Female , Humans , Mice , Neoplasm Metastasis
4.
Cancer Sci ; 107(9): 1206-14, 2016 Sep.
Article in English | MEDLINE | ID: mdl-27384869

ABSTRACT

Although it has been suspected that inflammation is associated with increased tumor metastasis, the exact type of immune response required to initiate cancer progression and metastasis remains unknown. In this study, by using an in vivo tumor progression model in which low tumorigenic cancer cells acquire malignant metastatic phenotype after exposure to inflammation, we found that IL-17A is a critical cue for escalating cancer cell malignancy. We further demonstrated that the length of exposure to an inflammatory microenvironment could be associated with acquiring greater tumorigenicity and that IL-17A was critical for amplifying such local inflammation, as observed in the production of IL-1ß and neutrophil infiltration following the cross-talk between cancer and host stromal cells. We further determined that γδT cells expressing Vδ1 semi-invariant TCR initiate cancer-promoting inflammation by producing IL-17A in an MyD88/IL-23-dependent manner. Finally, we identified CD30 as a key molecule in the inflammatory function of Vδ1T cells and the blockade of this pathway targeted this cancer immune-escalation process. Collectively, these results reveal the importance of IL-17A-producing CD30(+) Vδ1T cells in triggering inflammation and orchestrating a microenvironment leading to cancer progression.


Subject(s)
Inflammation/immunology , Inflammation/metabolism , Interleukin-17/biosynthesis , Ki-1 Antigen/metabolism , Neoplasms/etiology , Neoplasms/metabolism , T-Lymphocyte Subsets/immunology , T-Lymphocyte Subsets/metabolism , Animals , Cell Line, Tumor , Disease Models, Animal , Disease Progression , Immunity , Inflammation/complications , Mice , Mice, Knockout , Models, Biological , Neoplasms/pathology , Tumor Microenvironment/immunology
5.
Cancer Sci ; 106(7): 867-74, 2015 Jul.
Article in English | MEDLINE | ID: mdl-25940224

ABSTRACT

Although the importance of the host tissue microenvironment in cancer progression and metastasis has been established, the spatiotemporal process establishing a cancer metastasis-prone tissue microenvironment remains unknown. In this study, we aim to understand the immunological character of a metastasis-prone microenvironment in a murine 4T1 breast tumor model, by using the activation of nuclear factor-κb (NF-κB) in cancer cells as a sensor of inflammatory status and by monitoring its activity by bioluminescence imaging. By using a 4T1 breast cancer cell line stably expressing an NF-κB/Luc2 reporter gene (4T1 NF-κB cells), we observed significantly increased bioluminescence approximately 7 days after metastasis-prone orthotopic mammary fat-pad inoculation but not ectopic s.c. inoculation of 4T1 NF-κB cells. Such in vivo NF-κB activation within the fat-pad 4T1 tumor was diminished in immune-deficient SCID or nude mice, or T cell-depleted mice, suggesting the requirement of host T cell-mediated immune responses. Given the fat-pad 4T1 tumor expressed higher inflammatory mediators in a T cell-dependent mechanism compared to the s.c. tumor, our results imply the importance of the surrounding tissue microenvironment for inflaming tumors by collaborating with T cells to instigate metastatic spread of 4T1 breast cancer cells.


Subject(s)
Lung Neoplasms/immunology , Mammary Neoplasms, Animal/immunology , T-Lymphocytes/immunology , Tumor Microenvironment/immunology , Animals , Cell Line, Tumor , Cell Movement , Female , Lung Neoplasms/secondary , Lymphocytes, Tumor-Infiltrating/immunology , Macrophages/immunology , Mammary Neoplasms, Animal/pathology , Mice, Inbred BALB C , Mice, Nude , Mice, SCID , Neoplasm Transplantation
6.
Biochem Biophys Res Commun ; 458(4): 856-61, 2015 Mar 20.
Article in English | MEDLINE | ID: mdl-25701783

ABSTRACT

Epidermal growth factor receptor (EGFR) mutation is one of the hallmarks of cancer progression and resistance to anticancer therapies, particularly non-small cell lung carcinomas (NSCLCs). In contrast to the canonical EGFR activation in which tyrosine residues are engaged, we have demonstrated that the non-canonical pathway is triggered by phosphorylation of serine and threonine residues through p38 and ERK MAPKs, respectively. The purpose of this study is to investigate the role of non-canonical EGFR pathway in resistance mechanism against cisplatin treatment. Wild type and mutated (exon 19 deletion) EGFR-expressing cells responded similarly to cisplatin by showing MAPK-mediated EGFR phosphorylation. It is interesting that internalization mechanism of EGFR was switched from tyrosine kinase-dependent to p38-dependent fashions, which is involved in a survival pathway that counteracts cisplatin treatment. We therefore introduce a potential combinatorial therapy composed of p38 inhibition and cisplatin to block the activation of EGFR, therefore inducing cancer cell death and apoptosis.


Subject(s)
Antineoplastic Agents/pharmacology , Carcinoma, Non-Small-Cell Lung/drug therapy , Carcinoma, Non-Small-Cell Lung/genetics , Cisplatin/pharmacology , ErbB Receptors/genetics , Lung Neoplasms/drug therapy , Lung Neoplasms/genetics , Apoptosis/drug effects , Carcinoma, Non-Small-Cell Lung/pathology , Cell Line, Tumor , Drug Resistance, Neoplasm , ErbB Receptors/metabolism , Humans , Lung/drug effects , Lung/metabolism , Lung/pathology , Lung Neoplasms/pathology , MAP Kinase Signaling System/drug effects , Mutation , Phosphorylation/drug effects
7.
Helicobacter ; 20(5): 381-9, 2015 Oct.
Article in English | MEDLINE | ID: mdl-25704183

ABSTRACT

BACKGROUND: The interaction of Helicobacter pylori with gastric epithelial cells can result in the activation of transcription factor NF-κB via TGF-ß-activated kinase 1 (TAK1). In this study, we have demonstrated the role of H. pylori in the activation of EGFR via TAK1-mediated phosphorylation of p38. MATERIALS AND METHODS: Gastric epithelial AGS or MKN-45 cells were co-cultured with wild-type or cagA(-) H. pylori strains. H. pylori was added to the cells, and the activation of EGFR, p65 (NF-κB) subunit, p38, ERK, and TAK1 was examined by Western blotting. Infected cells were pretreated with or without ligands, chemical inhibitors, anti-HB-EGF antibody, and siRNAs to evaluate the effects on phosphorylation of various EGFR residues. Fluorescence microscopy and flow cytometry were performed to detect the internalization of EGFR. RESULTS: Incubating cells with wild-type and CagA(-) H. pylori strains resulted in the rapid and transient phosphorylation of serine residues of EGFR. RNAi experiments using siRNA against TAK1 and p38 pathways blocked the phosphorylation of serine residue. Immunofluorescence and flow cytometry revealed that EGFR was internalized in H. pylori-infected cells after EGFR phosphorylation in a p38-dependent manner. In contrast, pretreatment with gefitinib and anti-HB-EGF antibody did not block both the phosphorylation and internalization of EGFR. CONCLUSION: Helicobacter pylori induces internalization of EGFR via novel TAK1-p38-serine activation pathway which is independent of HB-EGF. The interaction between TAK1 and EGFR in H. pylori-infected cells might open new dimensions in understanding H. pylori-associated gastric carcinogenesis.


Subject(s)
ErbB Receptors/metabolism , Helicobacter pylori/metabolism , Host-Pathogen Interactions , MAP Kinase Kinase Kinases/metabolism , Protein Processing, Post-Translational , Serine/metabolism , p38 Mitogen-Activated Protein Kinases/metabolism , Blotting, Western , Cell Line , Coculture Techniques , Flow Cytometry , Humans , Microscopy, Fluorescence , Phosphorylation
8.
Biol Pharm Bull ; 38(1): 109-15, 2015.
Article in English | MEDLINE | ID: mdl-25744466

ABSTRACT

Cinnamomum cassia is widely employed for gastrointestinal complaints such as dyspepsia, flatulence, diarrhea, and vomiting. Studies report cinnamaldehyde (CM) as a major active constituent of cinnamon. The aim of this study was to evaluate the anti-inflammatory mechanism of CM on Helicobacter (H.) pylori-infected gastric epithelial cells in order to validate cinnamon traditional use in gastrointestinal (GI)-related disorders. AGS/MKN-45 cells and H. pylori (193C) were employed for co-culture experiments. Anti-H. pylori cytotoxic and anti-adhesion activity of CM were determined. Enzyme linked immunosorbent assay, real time polymerase chain reaction analysis and immunoblotting were used to measure the effect on interleukin-8 (IL-8) secretion/expression. The effect on activation of nuclear factor kappa B (NF-κB) was determined by immunoblot analysis. The non-cytotoxic CM (≤125 µM) was also non-bactericidal at the given time, suggesting the effect in H. pylori/cell co-culture system was not due to alteration in H. pylori viability or the toxicity to the cells. Also, CM did not show any anti-adhesion effect against H. pylori/cell co-culture. However, pre-incubation of the cells with CM significantly inhibited the IL-8 secretion/expression from H. pylori-infected cells (p<0.01). In addition, CM suppressed H. pylori-induced NF-κB activation and prevented degradation of inhibitor (I)-κB This study provides evidence that the anti-inflammatory effect of C. cassia on H. pylori-infected gastric cells is due to blockage of the NF-κB pathway by cinnamaldehyde. This agent can be considered as a potential candidate for in vivo and clinical studies against various H. pylori related gastric pathogenic processes.


Subject(s)
Acrolein/analogs & derivatives , Anti-Inflammatory Agents/pharmacology , Epithelial Cells/drug effects , Helicobacter pylori , Acrolein/pharmacology , Cell Line , Cell Line, Tumor , Epithelial Cells/metabolism , Epithelial Cells/microbiology , Gastritis/drug therapy , Helicobacter Infections/drug therapy , Helicobacter pylori/drug effects , Humans , Interleukin-8/metabolism , NF-kappa B/metabolism
9.
Cancer Sci ; 105(3): 281-9, 2014 Mar.
Article in English | MEDLINE | ID: mdl-24344732

ABSTRACT

Although the heterogeneities of epithelial and mesenchymal-transitioned cancer cells are often observed within the tumor microenvironment, the biological significance of the interaction between epithelial cancer cells and mesenchymal-transitioned cancer cells is not yet understood. In this study, we show that the mesenchymal-transitioned cancer cells instigate the invasive ability and metastatic potential of the neighboring epithelial cancer cells in vitro and in vivo. We identify WNT3 and WNT5B as critical factors secreted from Transforming growth factor-induced mesenchymal cancer cells for instigating the epithelial cancer cell invasion along with the induction of secondary EMT phenotype. These results shed light on the significance of cancer heterogeneity and the interaction between epithelial and mesenchymal-transitioned cancer cells within the tumor microenvironment in promoting metastatic disease through the WNT-dependent mechanism.


Subject(s)
Epithelial Cells/physiology , Lung Neoplasms/pathology , Wnt Proteins/physiology , Wnt3 Protein/physiology , Animals , Cell Line, Tumor , Cell Movement , Coculture Techniques , Epithelial-Mesenchymal Transition , Humans , Mice , Mice, Inbred ICR , Neoplasm Invasiveness , Neoplasm Transplantation , Wnt Proteins/metabolism , Wnt3 Protein/metabolism
10.
Cancer Sci ; 105(7): 788-94, 2014 Jul.
Article in English | MEDLINE | ID: mdl-24750242

ABSTRACT

Although epidermal growth factor receptor (EGFR)-tyrosine kinase inhibitors (EGFR-TKI), including gefitinib, provide a significant clinical benefit in non-small-cell lung cancer (NSCLC) patients, the acquisition of drug resistance has been known to limit the efficacy of EGFR-TKI therapy. In this study, we demonstrated the involvement of EGF-EGFR signaling in NSCLC cell migration and the requirement of RAC1 in EGFR-mediated progression of NSCLC. We showed the significant role of RAC1 pathway in the cell migration or lamellipodia formation by using gene silencing of RAC1 or induction of constitutive active RAC1 in EGFR-mutant NSCLC cells. Importantly, the RAC1 inhibition suppressed EGFR-mutant NSCLC cell migration and growth in vitro, and growth in vivo even in the gefitinib-resistant cells. In addition, these suppressions by RAC1 inhibition were mediated through MEK or PI3K independent mechanisms. Collectively, these results open up a new opportunity to control the cancer progression by targeting the RAC1 pathway to overcome the resistance to EGFR-TKI in NSCLC patients.


Subject(s)
Carcinoma, Non-Small-Cell Lung/drug therapy , Lung Neoplasms/drug therapy , Quinazolines/pharmacology , rac1 GTP-Binding Protein/antagonists & inhibitors , rac1 GTP-Binding Protein/metabolism , Aminoquinolines/pharmacology , Animals , Antineoplastic Agents/pharmacology , Carcinoma, Non-Small-Cell Lung/genetics , Carcinoma, Non-Small-Cell Lung/metabolism , Carcinoma, Non-Small-Cell Lung/pathology , Cell Line, Tumor/drug effects , Cell Movement/genetics , Drug Resistance, Neoplasm/genetics , ErbB Receptors/antagonists & inhibitors , ErbB Receptors/genetics , ErbB Receptors/metabolism , Female , Gefitinib , Gene Silencing , Humans , Lung Neoplasms/genetics , Lung Neoplasms/metabolism , Lung Neoplasms/pathology , Mice , Mutation , Protein Kinase Inhibitors/pharmacology , Pyrimidines/pharmacology , Signal Transduction , Xenograft Model Antitumor Assays , rac1 GTP-Binding Protein/genetics
11.
Microvasc Res ; 91: 73-83, 2014 Jan.
Article in English | MEDLINE | ID: mdl-24316421

ABSTRACT

Microscopic bioimaging of blood flow and distribution of cancer cells in lungs is essential to analyze mechanism of lung metastasis. Such cancer metastasis has been well known to induce hypercoagulable states and thrombosis. In histopathological tissue sections, however, it has been difficult to capture rapid phenomenon of thrombus formation due to technical problems associated with much less retention of soluble serum components as well as dynamic histological features reflecting their living states. In this study, to achieve bioimaging of both hypercoagulable states and thrombosis induced by early metastasis of mouse B16-BL6 melanoma, "in vivo cryotechnique" (IVCT) was used, which retained soluble components at their original sites. Glutathione-coated quantum dots (QDs) were subsequently injected after melanoma cells via right ventricles to examine plasma flow with fluorescence emission. At 5s after the melanoma injection, melanoma cells were mostly stacked and intruded in alveolar capillaries with changing their shapes. Assembly of platelets initially appeared at 1min, and they aggregated around the stacked melanoma cells at 5min. Such aggregated platelets were immunopositive for both phospho-tyrosine 418 and 527 of Src, indicating their partial signal activation. Fibrin monomers were also immunolocalized around both melanoma cells and platelet aggregates, and massive immunoreaction deposits of fibrinogen were also detected near the same areas, but more strongly detected around the melanoma cells, indicating initial thrombus formation. In those areas, QDs were rarely detected, probably because of the lack of blood supply. Thus, IVCT revealed histopathological features of initial thrombosis under their circulatory conditions.


Subject(s)
Lung/blood supply , Melanoma/pathology , Microcirculation , Thrombosis/physiopathology , Animals , Blood Coagulation , Blood Platelets/metabolism , Cell Line, Tumor , Fibrinogen/chemistry , Freezing , Glutathione/chemistry , Lung/pathology , Lung Neoplasms/secondary , Male , Melanoma, Experimental/pathology , Mice , Mice, Inbred C57BL , Neoplasm Metastasis , Neoplasm Transplantation , Platelet Activation , Platelet Aggregation , Quantum Dots , src-Family Kinases/metabolism
12.
BMC Cancer ; 14: 949, 2014 Dec 15.
Article in English | MEDLINE | ID: mdl-25495942

ABSTRACT

BACKGROUND: Inhibition of metastasis through upregulation of immune surveillance is a major purpose of chemokine gene therapy. In this study, we focused on a membrane-bound chemokine CXCL16, which has shown a correlation with a good prognosis for colorectal cancer (CRC) patients. METHODS: We generated a CXCL16-expressing metastatic CRC cell line and identified changes in TNF and apoptosis-related factors. To investigate the effect of CXCL16 on colorectal liver metastasis, we injected SL4-Cont and SL4-CXCL16 cells into intraportal vein in C57BL/6 mice and evaluated the metastasis. Moreover, we analyzed metastatic liver tissues using flow cytometry whether CXCL16 expression regulates the infiltration of M1 macrophages. RESULTS: CXCL16 expression enhanced TNF-α-induced apoptosis through activation of PARP and the caspase-3-mediated apoptotic pathway and through inactivation of the NF-κB-mediated survival pathway. Several genes were changed by CXCL16 expression, but we focused on IRF8, which is a regulator of apoptosis and the metastatic phenotype. We confirmed CXCL16 expression in SL4-CXCL16 cells and the correlation between CXCL16 and IRF8. Silencing of IRF8 significantly decreased TNF-α-induced apoptosis. Liver metastasis of SL4-CXCL16 cells was also inhibited by TNF-α-induced apoptosis through the induction of M1 macrophages, which released TNF-α. Our findings suggest that the accumulation of M1 macrophages and the enhancement of apoptosis by CXCL16 might be an effective dual approach against CRC liver metastasis. CONCLUSIONS: Collectively, this study revealed that CXCL16 regulates immune surveillance and cell signaling. Therefore, we provide the first evidence of CXCL16 serving as an intracellular signaling molecule.


Subject(s)
Apoptosis/genetics , Chemokines, CXC/genetics , Colorectal Neoplasms/genetics , Colorectal Neoplasms/pathology , Liver Neoplasms/secondary , Macrophages/metabolism , Receptors, Scavenger/genetics , Tumor Necrosis Factor-alpha/metabolism , Animals , Apoptosis/drug effects , Cell Line, Tumor , Cell Membrane/metabolism , Chemokine CXCL16 , Chemokines, CXC/metabolism , Colorectal Neoplasms/immunology , Colorectal Neoplasms/metabolism , Drug Resistance/genetics , Gene Expression , Gene Silencing , Interferon Regulatory Factors/genetics , Interferon Regulatory Factors/metabolism , Macrophages/immunology , Mice , RNA Interference , Receptors, Scavenger/metabolism , Tumor Necrosis Factor-alpha/pharmacology
13.
J Nat Prod ; 77(4): 976-82, 2014 Apr 25.
Article in English | MEDLINE | ID: mdl-24592993

ABSTRACT

Campechic acids A (1) and B (2), two new polyketides, were isolated from the culture extract of Streptomyces sp., and their structures were determined by NMR and MS spectroscopic analysis. Campechic acids are polyether-polyketides functionalized by two tetrahydrofuran rings, an enolized 1,3-diketone, and multiple methyl substitutions. Absolute configuration of nine stereogenic centers in 1, except for four chiral centers in the cyclic ether moieties, was determined by the 1H NMR anisotropy method in combination with chemical degradation. Campechic acids exhibited potent inhibitory effects on tumor cell invasion with IC50 values in the nanomolar to submicromolar range.


Subject(s)
Antineoplastic Agents/isolation & purification , Polyketides/isolation & purification , Streptomyces/chemistry , Antineoplastic Agents/chemistry , Antineoplastic Agents/pharmacology , Bacillus subtilis/drug effects , Candida albicans/drug effects , Drug Screening Assays, Antitumor , Escherichia coli/drug effects , Humans , Inhibitory Concentration 50 , Mexico , Microbial Sensitivity Tests , Micrococcus luteus/drug effects , Molecular Structure , Nuclear Magnetic Resonance, Biomolecular , Polyketides/chemistry , Polyketides/pharmacology , Soil Microbiology , Staphylococcus aureus/drug effects
14.
Cancer Sci ; 104(5): 558-62, 2013 May.
Article in English | MEDLINE | ID: mdl-23347256

ABSTRACT

Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) has been recognized as a promising target for cancer therapy because it can induce apoptotic cell death in tumor cells but not normal cells. Although TRAIL shows specific tumoricidal activity, resistance to TRAIL-induced apoptosis in some tumor cells has been considered a clinical obstacle of its application. It has been shown that TRAIL provides inflammatory signals that may contribute to the TRAIL-resistance of cancer cells; however, it is not known whether TRAIL itself is involved in malignant cancer cell behavior. In the present study, we examined the functional role of TRAIL in B16F10 mouse melanoma cells, which are totally insensitive to TRAIL-induced apoptosis. By establishing B16F10 cells stably expressing the nuclear factor-κB (NFκB)-luciferase reporter gene, we found that TRAIL can activate NFκB through its death receptor DR5 in B16F10 cells. Furthermore, TRAIL-DR5 interaction not only promoted malignant behaviors of B16F10 cells, such as cell proliferation and MMP-9 production, but also induced lung metastasis of B16F10 cells in vivo. These findings may imply a contrary role for the TRAIL-DR5 pathway in the inflammatory tumor microenvironment, in its ability to induce the metastatic potential of B16F10 melanoma cells instead of inducing apoptosis.


Subject(s)
Melanoma, Experimental/metabolism , Melanoma, Experimental/pathology , NF-kappa B/metabolism , Receptors, TNF-Related Apoptosis-Inducing Ligand/metabolism , TNF-Related Apoptosis-Inducing Ligand/metabolism , Animals , Apoptosis/physiology , Cell Growth Processes/physiology , Cell Line, Tumor , Inflammation/genetics , Inflammation/metabolism , Lung Neoplasms/genetics , Lung Neoplasms/metabolism , Lung Neoplasms/secondary , Matrix Metalloproteinase 9/genetics , Matrix Metalloproteinase 9/metabolism , Melanoma, Experimental/genetics , Mice , Mice, Inbred C57BL , NF-kappa B/genetics , Neoplasm Metastasis , Receptors, TNF-Related Apoptosis-Inducing Ligand/genetics , TNF-Related Apoptosis-Inducing Ligand/genetics , Tumor Microenvironment/physiology
15.
Cancer Sci ; 104(10): 1315-22, 2013 Oct.
Article in English | MEDLINE | ID: mdl-23822636

ABSTRACT

We have recently identified tumor necrosis factor (TNF)-α-induced phosphorylation of epidermal growth factor receptor (EGFR) at Thr-669 and Ser-1046/1047 via ERK and p38 pathways, respectively. In the present study, we investigated the roles of ligand-induced phosphorylation of serine and threonine residues in EGFR-overexpressing MDA-MB-468 breast cancer cells. Epidermal growth factor and heregulin, an ErbB3 ligand, induced the phosphorylation of Thr-669 and Ser-1046/1047. Inversely, constitutive tyrosine phosphorylation of the C-terminal domain, including Tyr-1068, was significantly downregulated on ligand stimulation. Inhibition of the ERK pathway by U0126 blocked ligand-induced Thr-669 phosphorylation as well as Tyr-1068 dephosphorylation. Downregulation of constitutive tyrosine phosphorylation of EGFR in HEK293 cells stably expressing the wild type was abolished by substitution of Thr-669 for Ala. In an asymmetric EGFR homodimer structure, one Thr-669 in the receiver kinase of the dimer was involved in downregulation. Similarly, Thr-669 in an EGFR-ErbB3 heterodimer also participated in tyrosine dephosphorylation. These results indicate that ERK-mediated Thr-669 phosphorylation suppresses constitutive tyrosine phosphosphorylation in the homo- and heterodimer asymmetric conformations of the EGFR.


Subject(s)
ErbB Receptors/metabolism , MAP Kinase Signaling System , Neoplasm Proteins/metabolism , Phosphothreonine/metabolism , Protein Processing, Post-Translational , Adenocarcinoma/enzymology , Breast Neoplasms/enzymology , Butadienes/pharmacology , Cell Line, Tumor/enzymology , Dimerization , Epidermal Growth Factor/pharmacology , ErbB Receptors/chemistry , Feedback, Physiological , Female , Humans , Ligands , Neoplasm Proteins/chemistry , Neuregulin-1/pharmacology , Nitriles/pharmacology , Phosphorylation , Protein Conformation , Protein Kinase Inhibitors/pharmacology , Recombinant Proteins/pharmacology , Tumor Necrosis Factor-alpha/pharmacology
16.
J Nat Prod ; 76(7): 1337-42, 2013 Jul 26.
Article in English | MEDLINE | ID: mdl-23855338

ABSTRACT

An optically inactive C-20 bisacetylenic alcohol, (4E,16E)-icosa-4,16-diene-1,19-diyne-3,18-diol, was isolated from a marine sponge Callyspongia sp. as a result of screening of antilymphangiogenic agents from marine invertebrates. An optical resolution using chiral-phase HPLC gave each enantiomer, (-)-1 and (+)-2. Because the natural and synthetic enantiomers 1 and 2 showed different biological properties, we investigated the structure-activity relationships of bisacetylenic alcohols using 11 synthetic derivatives, and it is clarified that the essential structural unit for antiproliferative activity is the "1-yn-3-ol" on both termini and that there is a minimum chain length that connects the "1-yn-3-ol" moieties.


Subject(s)
Alcohols/isolation & purification , Callyspongia/chemistry , Diynes/isolation & purification , Alcohols/chemistry , Alcohols/pharmacology , Animals , Diynes/chemistry , Diynes/pharmacology , Japan , Marine Biology , Molecular Structure , Oceans and Seas , Stereoisomerism , Structure-Activity Relationship
17.
Int J Hyperthermia ; 29(7): 688-95, 2013 Nov.
Article in English | MEDLINE | ID: mdl-24028082

ABSTRACT

PURPOSE: Transforming growth factor-ß-activated kinase 1 (TAK1) plays a role in inhibiting apoptosis in response to multiple stresses. In the present study, we investigated the role of TAK1 in cell death induced by heat stress (HS). MATERIALS AND METHODS: TAK1 knockdown HeLa cells and their parental cells were exposed to HS at 44 °C for 15, 30, 45 min followed by colony formation assay. Heat shock proteins (HSPs) induction, NF-κB phosphorylation, and caspase-3 cleavage were estimated by western blotting using specific antibodies. Global gene expression analysis was performed using the GeneChip® system. The anti-apoptotic roles of the identified genes were elucidated using small interfering RNAs targeting those genes. RESULTS: Heat sensitivity estimated by colony formation assay and caspase-3 cleavage increased in TAK1 knockdown cells. This sensitisation was not due to alterations in HSP induction or NF-κB phosphorylation as the expression levels of these proteins did not differ significantly between the TAK1 knockdown and the parent cells after HS exposure. The GeneChip® analysis revealed differences in gene expression between both cell variants after HS exposure and defined the genetic network associated with cell death. TNF-α interacting protein 3 (TNFAIP3) and Interleukin 8 (IL-8) are two of the identified genes. RNA interference against these genes increased the cleavage of caspase-3 and cell death after HS exposure. CONCLUSION: Our findings reveal the role of TAK1 in thermoresistance and show that the mediation is independent of NF-κB phosphorylation but is dependent on TNFAIP3 and IL-8 induction.


Subject(s)
DNA-Binding Proteins/genetics , Heat-Shock Response/physiology , Interleukin-8/genetics , Intracellular Signaling Peptides and Proteins/genetics , MAP Kinase Kinase Kinases/genetics , Nuclear Proteins/genetics , Cell Survival/physiology , Gene Expression Profiling , Gene Knockdown Techniques , HeLa Cells , Heat-Shock Proteins/genetics , Heat-Shock Proteins/metabolism , Humans , MAP Kinase Kinase Kinases/metabolism , NF-kappa B/metabolism , RNA, Messenger/metabolism , Tumor Necrosis Factor alpha-Induced Protein 3
18.
Biol Pharm Bull ; 36(1): 152-7, 2013.
Article in English | MEDLINE | ID: mdl-23302649

ABSTRACT

Metastasis occurs when cancer cells detach from a tumor, travel to distant sites in the body and develop into tumors in these new locations. Most cancer patients die from metastases. Among the various forms of cancer metastasis, lymphatic metastasis is an important determinant in cancer treatment and staging. In this study, we investigated lymphangiogenesis inhibitors from crude drugs used in Japan and Korea. The three crude drugs Saussureae Radix, Psoraleae Semen and Aurantti Fructus Immaturus significantly inhibited the proliferation of temperature-sensitive rat lymphatic endothelial (TR-LE) cells in vitro. By a chromatographic method using bioassay-guided fractionation methods, costunolide (1) and dehydrocostus lactone (2) from S. Radix, p-hydroxybenzaldehyde (3), psoralen (4), angelicin (5), psoracorylifol D (6), isobavachalone (7), bavachinin (8) Δ(3),2-hydroxybakuchiol (9) and bakuchiol (10) from P. Semen and cis-octadecyl ferulate (11), (2R)-3ß,7,4'-trihydroxy-5-methoxy-8-(γ,γ-dimethylallyl)-flavanone (12), (2S)-7,4'-dihydroxy-5-methoxy-8-(γ,γ-dimethylallyl)-flavanone (13) and umbelliferone (14) from A. F. Immaturus were obtained. Three compounds (compounds 11-13) from A. F. Immaturus were isolated for the first time from this medicinal plant. Among isolated compounds, ten compounds (compounds 1, 2, 6-12, 13) showed an inhibitory effect on the proliferation and the capillary-like tube formation of TR-LE cells. In addition, all compounds except compound 12 showed selective inhibition of the proliferation of TR-LE cells compared to Hela and Lewis lung carcinoma (LLC) cells. These compounds might offer clinical benefits as lymphangiogenesis inhibitors and may be good candidates for novel anti-cancer and anti-metastatic agents.


Subject(s)
Angiogenesis Inhibitors/pharmacology , Plant Extracts/pharmacology , Poncirus , Psoralea , Saussurea , Animals , Cell Cycle/drug effects , Cell Line, Tumor , Cell Proliferation/drug effects , Endothelial Cells/cytology , Endothelial Cells/drug effects , Endothelial Cells/physiology , Fruit , HeLa Cells , Humans , Japan , Lymphangiogenesis/drug effects , Plant Roots , Rats , Republic of Korea , Seeds
19.
Planta Med ; 79(2): 157-62, 2013 Jan.
Article in English | MEDLINE | ID: mdl-23225367

ABSTRACT

Chemical investigation on the 75% ethanol extract of the roots of Streptocaulon juventas afforded two new cardiac glycosides, digitoxigenin 3-O-[O-ß-D-glucopyranosyl-(1 → 4)-2-O-acetyl-ß-D-digitalopyranoside] (1) and periplogenin 3-O-[O-ß-D-glucopyranosyl-(1 → 4)-O-ß-D-glucopyranosyl-(1 → 4)-ß-D-cymaropyranoside] (2), and thirteen known cardenolides. Structures were elucidated by spectral methods. This is the first report of the isolation of compounds 3, 10, 14, and 15 from plants of the Streptocaulon genus, while 4, 11, and 12 are hitherto unreported from Streptocaulon juventas. All the compounds were in vitro evaluated for their cytotoxic activities against the A549 cell line, and seven effective cardiac glycosides were screened against the PC-9 cell line by WST assay, which also showed strong antiproliferation activities. Moreover, the characteristic morphological changes in PC-9 cells treated with cardenolides indicated cell inhibition due to apoptosis. These results revealed that these compounds possessed potential antitumor activities.


Subject(s)
Antineoplastic Agents, Phytogenic/pharmacology , Apocynaceae/chemistry , Cardenolides/pharmacology , Cardiac Glycosides/pharmacology , Antineoplastic Agents, Phytogenic/chemistry , Antineoplastic Agents, Phytogenic/isolation & purification , Apoptosis/drug effects , Cardenolides/chemistry , Cardenolides/isolation & purification , Cardiac Glycosides/chemistry , Cardiac Glycosides/isolation & purification , Cell Line, Tumor , Cell Proliferation/drug effects , Cell Survival/drug effects , Humans , Magnetic Resonance Spectroscopy , Medicine, East Asian Traditional , Molecular Structure , Plant Roots/chemistry , Plants, Medicinal
20.
Chem Pharm Bull (Tokyo) ; 61(12): 1318-22, 2013.
Article in English | MEDLINE | ID: mdl-24436964

ABSTRACT

A new pyridine alkaloid-coupled iridoid glucoside, dipasperoside A (1), and 20 known compounds (2­21) were isolated from a water extract of Dipsacus asper roots. Compound 1 possessed a unique structural feature with a nicotinic acid nucleus coupled through C-5 with C-7 of a secoiridoid/iridoid glucoside dimer, and esterified with a C-7 hydroxyl group of an iridoid glucoside monomer. All isolates were evaluated for their inhibitory activity against nitric oxide (NO) production in a lipopolysaccharide (LPS)-activated murine macrophage cell line, RAW264.7.


Subject(s)
Alkaloids/analysis , Anti-Inflammatory Agents/analysis , Dipsacaceae/chemistry , Glucosides/analysis , Iridoids/analysis , Pyridines/analysis , Alkaloids/isolation & purification , Alkaloids/pharmacology , Animals , Anti-Inflammatory Agents/isolation & purification , Anti-Inflammatory Agents/pharmacology , Cell Line , Glucosides/isolation & purification , Glucosides/pharmacology , Iridoids/isolation & purification , Iridoids/pharmacology , Lipopolysaccharides/immunology , Macrophages/drug effects , Macrophages/immunology , Mice , Nitric Oxide/immunology , Plant Roots/chemistry , Pyridines/isolation & purification , Pyridines/pharmacology
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