ABSTRACT
Enterotoxigenic Escherichia coli (ETEC) has consistently been the predominant bacterial cause of diarrhea in many birth cohort- and hospital-based studies conducted in Egypt. We evaluated the pathogenicity of ETEC isolates in a birth cohort of children living in a rural community in Egypt. Between 2004 and 2007, we enrolled and followed 348 children starting at birth until their second year of life. A stool sample and two rectal swabs were collected from children during twice-weekly visits when they presented with diarrhea and were collected every 2 weeks if no diarrhea was reported. From routine stool cultures, five E. coli-like colonies were screened for ETEC enterotoxins using a GM1 enzyme-linked immunosorbent assay (ELISA). The isolates were screened against a panel of 12 colonization factor antigens (CFAs) by a dot blot assay. A nested case-control study evaluated the association between initial or repeat excretion of ETEC and the occurrences of diarrhea. The pathogenicity of ETEC was estimated in symptomatic children compared to that in asymptomatic controls. ETEC was significantly associated with diarrhea (crude odds ratio, 1.37; 95% confidence interval [CI], 1.24 to 1.52). The distribution of ETEC enterotoxins varied between the symptomatic children (44.2% heat-labile toxin [LT], 38.5% heat-stable toxin [ST], and 17.3% LT/ST) and asymptomatic children (55.5% LT, 34.6% ST, and 9.9% LT/ST) (P < 0.001). The CFAs CFA/I (n = 61), CS3 (n = 8), CS1 plus CS3 (n = 24), CS2 plus CS3 (n = 18), CS6 (n = 45), CS5 plus CS6 (n = 11), CS7 (n = 25), and CS14 (n = 32) were frequently detected in symptomatic children, while CS6 (n = 66), CS12 (n = 51), CFA/I (n = 43), and CS14 (n = 20) were detected at higher frequencies among asymptomatic children. While all toxin phenotypes were associated with diarrheal disease after the initial exposure, only ST and LT/ST-expressing ETEC isolates (P < 0.0001) were associated with disease in repeat infections. The role of enterotoxins and pathogenicity during repeat ETEC infections appears to be variable and dependent on the coexpression of specific CFAs.
Subject(s)
Diarrhea/microbiology , Enterotoxigenic Escherichia coli/classification , Enterotoxigenic Escherichia coli/isolation & purification , Escherichia coli Infections/microbiology , Case-Control Studies , Child, Preschool , Cohort Studies , Diarrhea/epidemiology , Egypt/epidemiology , Enterotoxigenic Escherichia coli/genetics , Enterotoxigenic Escherichia coli/physiology , Escherichia coli Infections/epidemiology , Feces/microbiology , Female , Humans , Immunoassay , Infant , Infant, Newborn , Male , Phenotype , Rural Population , Virulence Factors/analysisABSTRACT
A total of 220 enteroadherent Escherichia coli were identified from 729 Egyptian children with diarrhea using the HEp-2 adherence assay. Enteropathogenic E.coli (EPEC = 38) was common among children <6 months old and provoked vomiting, while diffuse-adhering E.coli (DAEC = 109) induced diarrheal episodes of short duration, and enteroaggregative E.coli (EAEC = 73) induced mild non-persistent diarrhea. These results suggest that EPEC is associated with infantile diarrhea in Egyptian children.
Subject(s)
Diarrhea, Infantile/epidemiology , Diarrhea, Infantile/microbiology , Enteropathogenic Escherichia coli/isolation & purification , Escherichia coli Infections/epidemiology , Escherichia coli Infections/microbiology , Escherichia coli/classification , Child , Child, Preschool , Diarrhea, Infantile/diagnosis , Egypt/epidemiology , Enteropathogenic Escherichia coli/genetics , Enzyme-Linked Immunosorbent Assay , Escherichia coli/isolation & purification , Escherichia coli Infections/diagnosis , Feces/microbiology , Female , Humans , Infant , Male , Phenotype , PrevalenceABSTRACT
Enterotoxigenic Escherichia coli (ETEC) is recognized to be a common cause of acute watery diarrhea in children from developing countries. Colonization factors (CFAs) have been identified predominantly in ETEC isolates secreting heat-stable enterotoxin (ST) or cosecreting ST with a heat-labile toxin (LT). We hypothesized that LT-only-secreting ETEC produces unique colonization factors not previously described in ST and LTST-secreting ETEC. A set of degenerate primers based on nucleotide sequence similarities between the major structural genes of CS20 (csnA), CS18 (fotA), CS12 (cswA), and porcine antigen 987 (fasA) was developed and used to screen a collection of 266 LT-secreting ETEC isolates in which no known CFA was detected. PCR-amplified products of different molecular masses were obtained from 49 (18.4%) isolates. Nucleotide sequence analysis of the PCR amplicons followed by GenBank nucleotide BLASTn analysis revealed five novel DNA sequences; translated amino acid BLASTx analysis confirmed sequence similarity to class 1b major structural proteins encoded by csnA, fotA, and fasA. Strains expressing the novel CFAs were phylotyped and analyzed using multilocus sequence typing (MLST; Achtman scheme), and the types detected were compared to those of a collection of archived global E. coli strains. In conclusion, application of the degenerate primer sets to ETEC isolates from surveillance studies increased the total number of ETEC isolates with detectable CFAs by almost 20%. Additionally, MLST analysis suggests that for many CFAs, there may be a requirement for certain genetic backgrounds to acquire and maintain plasmids carrying genes encoding CFAs.
Subject(s)
Adhesins, Bacterial/genetics , Enterotoxigenic Escherichia coli/genetics , Escherichia coli Proteins/genetics , Fimbriae, Bacterial/genetics , Bacterial Typing Techniques , Child , Child, Preschool , Cluster Analysis , DNA Primers/genetics , DNA, Bacterial/chemistry , DNA, Bacterial/genetics , Enterotoxigenic Escherichia coli/classification , Enterotoxigenic Escherichia coli/isolation & purification , Escherichia coli Infections/microbiology , Genotype , Humans , Molecular Sequence Data , Multilocus Sequence Typing , Phylogeny , Polymerase Chain Reaction , Sequence Analysis, DNAABSTRACT
To identify enteropathogens for vaccine development, we implemented clinic-based surveillance for severe pediatric diarrhea in Egypt's Nile River Delta. Over 2 years, a physician clinically evaluated and obtained stool samples for microbiology from patients with diarrhea and less than 6 years of age. In the first (N = 714) and second clinic (N = 561), respectively, 36% (N = 254) and 46% (N = 260) of children were infected with rotavirus, enterotoxigenic Escherichia coli (ETEC), Campylobacter, or Shigella. When excluding mixed rotavirus-bacterial infections, for the first and second clinic, 23% and 10% had rotavirus-associated diarrhea, and 14% and 17% had ETEC-associated diarrhea, respectively. Campylobacter-associated diarrhea was 1% and 3%, and Shigella-associated diarrhea was 2% and 1%, respectively, for the two clinics. Rotavirus-associated diarrhea peaked in late summer to early winter, while bacterial agents were prevalent during summer. Rotavirus-associated cases presented with dehydration, vomiting, and were often hospitalized. Children with Shigella- or Campylobacter-associated diarrhea reported as watery diarrhea and rarely dysentery. ETEC did not have any clinically distinct characteristics. For vaccine development and/or deployment, our study suggests that rotavirus is of principle concern, followed by ETEC, Shigella, and Campylobacter.
Subject(s)
Bacterial Infections/epidemiology , Diarrhea/microbiology , Diarrhea/virology , Population Surveillance , Rotavirus Infections/epidemiology , Bacterial Infections/microbiology , Child, Preschool , Diarrhea/epidemiology , Egypt/epidemiology , Female , Humans , Infant , Infant, Newborn , Male , SeasonsABSTRACT
Operation Bright Star (OBS) is a biennial, multinational exercise in Egypt involving 15000 US troops. Consistent with past observations in deployed troops, diarrhea is the most significant cause of morbidity. Focused efforts are ongoing to develop vaccines against the most common pathogens affecting our troops. As part of these efforts, diarrhea surveillance was conducted during OBS to monitor pathogens associated with illness and to identify new vaccine targets. A retrospective review was conducted of prior studies with similar methods. Soldiers with diarrhea presenting to the OBS clinic provided a stool sample that was inoculated into Carey-Blair transport media. Within 3 days, the Cary-Blair tubes were transported to the Naval Medical Research Unit no. 3 in Cairo where bacterial culture was performed. As part of the evaluation, 5 Escherichia coli-like colonies were collected and tested for toxin production using the GM1-ELISA. Toxin-positive isolates were further tested for colonization factors (CF) by a dot-blot assay using a standardized panel of monoclonal antibodies against CFA/I, CS1-CS7, CS17, CS8 (CFA/III), CS12 (PCFO159), and CS14 (PCFO166). Enterotoxigenic E. coli (ETEC) was the most frequently isolated pathogen during each OBS from which data were collected. The rate of ETEC-associated diarrhea ranged from 22% to 58%. Over time, there were dramatic shifts in the frequency and distribution of CFs. Over the 5 years of study, an increasing number of ETEC isolates had no known CF identified, and in 2001, only 40% of ETEC was associated with known CFs. The most commonly identified CF was CS6. Diarrheal disease, particularly ETEC, continues to be a common malady among US military personnel deployed to Egypt. We have identified ETEC CF types, especially CS6, which should be considered potential vaccine candidates. However, despite intensive testing, CFs could not be identified in most of the ETEC isolated, highlighting the need for further studies to identify novel CFs and alternative vaccine targets.
Subject(s)
Diarrhea/microbiology , Enterotoxins/metabolism , Escherichia coli/isolation & purification , Feces/microbiology , Military Personnel , Antibodies, Monoclonal , Bacterial Typing Techniques , Bacteriological Techniques , Desert Climate , Egypt , Escherichia coli/classification , Escherichia coli/metabolism , Humans , Military Medicine , Polymerase Chain Reaction , Retrospective Studies , Time Factors , United StatesABSTRACT
In a cross-sectional study of children <60 months old from Fayoum, Egypt, presenting with diarrhea, 46% (162/356) had detectable enteric pathogens. Bacterial pathogens were identified in 25% (89/356), whereas rotavirus and Cryptosporidium were detected in 21% (54/253) and 15% (39/253), respectively. Cryptosporidium is an important pathogen in this region.
Subject(s)
Cryptosporidiosis/complications , Diarrhea, Infantile/microbiology , Diarrhea, Infantile/virology , Diarrhea/microbiology , Diarrhea/virology , Population Surveillance , Chi-Square Distribution , Child, Preschool , Cross-Sectional Studies , Cryptosporidiosis/epidemiology , Diarrhea/epidemiology , Diarrhea, Infantile/epidemiology , Egypt/epidemiology , Humans , Infant , Infant, Newborn , Statistics, NonparametricABSTRACT
BACKGROUND: The aim of this study was to characterize phenotypically enterotoxins, colonization factors (CFs) and the antibiotic susceptibility of enterotoxigenic Escherichia coli (ETEC) strains isolated from cases of acute diarrhea that occurred in Europeans traveling to resorts in Mombasa, Kenya; this information is critical for the development of vaccines and empirical treatment. METHODS: Over a 1-year period from 1996 to 1997, five E. coli-like colonies were obtained from each of 463 cases with acute diarrhea. These strains were characterized for enterotoxins using GM-1 ELISA, for CFs using a dot-blot assay, and for antibiotic susceptibility using antibiotic disks. RESULTS: Of 164 strains characterized for ETEC phenotype, 30 (18%) expressed heat-labile toxin (LT) only, 83 (51%) heat-stable toxin (ST) only, and 51 (31%) both LT and ST. Analysis for CF expression demonstrated that 107 (65%) of the strains were positive for CFs, including CFA/IV (46%), CFA/II (35%), and CFA/I (5%), while less than 4% expressed less common CFs. All ETEC strains tested were resistant to erythromycin and sensitive to ceftriaxone. Over one-third of the strains were resistant to sulfamethoxazole-trimethoprim or tetracycline. Six strains were resistant to nalidixic acid; none of these were resistant to ciprofloxacin. CONCLUSIONS: Cumulatively, our findings indicate that ETEC in this region comprises a highly diverse group of bacterial enteropathogens, and that the development of prophylactic agents against ETEC faces major challenges because of this diversity.
Subject(s)
Diarrhea/microbiology , Enterotoxins/analysis , Escherichia coli Infections/microbiology , Escherichia coli/classification , Escherichia coli/pathogenicity , Travel , Anti-Bacterial Agents/pharmacology , Diarrhea/complications , Diarrhea/epidemiology , Drug Resistance, Bacterial , Escherichia coli/drug effects , Escherichia coli/genetics , Escherichia coli Infections/complications , Escherichia coli Infections/epidemiology , Female , Humans , Kenya/epidemiology , Male , PhenotypeABSTRACT
Enterotoxigenic Escherichia coli (ETEC) is a major health problem for travelers to the Middle East. During the autumn months of 2005, 2007, and 2009, U.S. military personnel participated in Operation Bright Star (OBS) exercises in Egypt. Out of 181 military personnel enrolled in a diarrheal surveillance study, E. coli-like colonies were isolated from 170 patients. Isolates were tested for the detection of ETEC enterotoxins and colonization factors (CFs) using phenotypic and genotypic methods. Additionally, we studied the secular trends of ETEC isolates obtained from OBS studies since 1999. ETEC was isolated from 51.2% and 60.0% of the patients based on enzyme-linked immunosorbent assay and polymerase chain reaction (PCR), respectively. Heat stable (ST) was the dominant enterotoxin detected followed by heat labile (LT) and LTST. Additionally, we detected a CF in 59.7% and 67.6% of the ETEC-positive isolates using dot blot and PCR assays, respectively. The predominant CF isolated was CS6 followed by CS3.
Subject(s)
Diarrhea/microbiology , Enterotoxigenic Escherichia coli/genetics , Escherichia coli Infections/microbiology , Diarrhea/epidemiology , Egypt , Enterotoxigenic Escherichia coli/isolation & purification , Enterotoxins/genetics , Epidemiological Monitoring , Escherichia coli Infections/epidemiology , Fimbriae Proteins/genetics , Genotype , Humans , Military Personnel , Phenotype , Prevalence , United States/ethnology , Virulence Factors/geneticsABSTRACT
INTRODUCTION: One approach to control enterotoxigenic Escherichia coli (ETEC) infections has been to develop vaccines focused on inducing protective immunity against surface expressed antigenic factors. One such factor is coli surface antigen 6 (CS6); ETEC isolates expressing CS6 may also simultaneously co-express surface antigens CS4 or CS5. However, there is little information regarding the inter-relationships of isolates expressing the CS6 antigen alone or in combination with CS4 or CS5. METHODOLOGY: A total of 62 CS6-associated ETEC isolates were evaluated for their antimicrobial susceptibility, mechanisms of resistance, toxin genes, colonization factor expression, and XbaI-pulsed-field gel electrophoretic profiles. RESULTS: We observed 46 XbaI profiles; 31 were exclusive to ETEC expressing CS6 alone and 15 among the ETEC co-expressing CS4 or CS5. Nearly half (47%) of these isolates were resistant to ampicillin, a third (37%) of the isolates were resistant to trimethoprim-sulfamethoxazole, and 24% of the isolates were tetracycline-resistant. A blaTEM gene was detected in 24 (83%) ampicillin-resistant isolates. Trimethoprim-sulfamethoxazole-resistant isolates (n = 23) carried either sulI (n = 1, 4%), sulII (n = 8, 35%) or both genes (n = 10, 43%); 4 had no detectable sul gene. CONCLUSIONS: Our results show a lack of clonality among Egypt CS6 E. coli isolates and supports the use and the further research on vaccines targeting this cell surface antigen.
Subject(s)
Antigens, Bacterial/analysis , Enterotoxigenic Escherichia coli/classification , Enterotoxigenic Escherichia coli/genetics , Escherichia coli Infections/microbiology , Escherichia coli Proteins/analysis , Anti-Bacterial Agents/pharmacology , Child, Preschool , Cluster Analysis , Egypt , Electrophoresis, Gel, Pulsed-Field , Enterotoxigenic Escherichia coli/drug effects , Enterotoxigenic Escherichia coli/isolation & purification , Genotype , Humans , Microbial Sensitivity Tests , Molecular Typing , Prospective Studies , Virulence Factors/geneticsABSTRACT
An Egyptian female with night sweats, headache, and back pain was diagnosed with acute brucellosis one week after returning from a North African country. Humoral immune responses to specific immunogenic proteins were investigated before and after treatment. ELISA was performed to detect levels of specific antibody (Ab) titers. Immunoblot analysis of Ab recognizing specific Brucella antigenic bands was also performed. IgA was detected on the day of disease onset. Specific agglutination titer was 1:160; it doubled three days later and treatment was implemented. Blood culture yielded Gram-negative coccobacilli after one month, confirmed as B. melitensis by AMOS-PCR. Immunoblotting revealed IgM Abs against two protein bands of 112 and 130-kDa observed only during the acute stage. On the other hand, the intensity of IgG Abs against 21 and 21.5-kDa protein bands positively correlated with the time of convalescence. Based on our observations we conclude that specific IgA levels may be used as an early diagnostic marker for Brucella and high molecular weight protein bands may be useful in the differentiation between acute and chronic brucellosis.
Subject(s)
Antibodies, Bacterial/blood , Brucella melitensis/isolation & purification , Brucellosis/diagnosis , Immunoglobulin A/blood , Agglutination Tests , Antigens, Bacterial/chemistry , Antigens, Bacterial/immunology , Brucella melitensis/genetics , Brucella melitensis/immunology , Enzyme-Linked Immunosorbent Assay , Female , Humans , Immunoblotting , Immunoglobulin G/blood , Immunoglobulin M/blood , Molecular Weight , Polymerase Chain ReactionABSTRACT
INTRODUCTION: Between 2004 and 2007, a birth cohort of Egyptian children was analysed to evaluate the epidemiology of enteric diseases. METHODOLOGY: A stool sample was collected from the study children every two weeks as well as whenever they experienced diarrhea. Samples were tested for routine bacterial pathogens as well as enteropathogenic viruses and parasites. A secondary goal of the study was to evaluate the burden of less commonly reported pathogens including Aeromonas hydrophila. RESULTS: Of the 348 study subjects, 79 had A. hydrophila isolated from their stool at some point during the study. Thirty-six children had exclusively symptomatic (S) infections while 33 had exclusively asymptomatic (AS) infections. However, 10 children had both S and AS infections. Among symptomatic cases, A. hydrophila was the sole pathogen isolated 36% of the time. An important aspect of A. hydrophila associated diarrhea was the high level of resistance to cephalosporins. CONCLUSION: Although relatively uncommon, A. hydrophila was found to be associated with diarrhea among children living in Egypt and was frequently multi-drug resistant.
Subject(s)
Aeromonas hydrophila/isolation & purification , Diarrhea/epidemiology , Gram-Negative Bacterial Infections/epidemiology , Diarrhea/microbiology , Diarrhea/pathology , Egypt/epidemiology , Feces/microbiology , Female , Gram-Negative Bacterial Infections/microbiology , Gram-Negative Bacterial Infections/pathology , Humans , Infant , Male , Prevalence , Rural PopulationABSTRACT
INTRODUCTION: Acute diarrhea continues to be a major cause of morbidity and mortality in children from developing countries. Determination of the frequency of diarrhea in an area, along with the proportion of disease caused by specific enteric agents of different origins, is considered the first step in controlling diarrheal diseases. METHODOLOGY: From 2005 to 2007, a hospital-based surveillance was conducted in two locations in Egypt to determine the causes of acute diarrhea in children younger than 5-years seeking treatment. Five additional enteric viral and parasitic pathogens were tested using commercially-available enzyme immunoassays (EIA) to re-evaluate the prevalence of diarrheal pathogens in undiagnosed cases. RESULTS: Adenovirus, astrovirus, norovirus and G. lamblia were detected as the sole pathogen in 2% (n=34), 3% (n=56), 9% (n=191) and 7% (n=146) of the cases, respectively. E. histolytica was never detected as the sole pathogen. The percentage of diarrheal cases with a known cause increased significantly, from 48% (n=1,006) to 74% (n=1,568) (P < 0.0001). CONCLUSION: In our study, the incorporation of immunoassays yielded useful data in identifying pathogens in previously pathogen-negative diarrhea cases.
Subject(s)
Adenoviridae/isolation & purification , Diarrhea/parasitology , Diarrhea/virology , Giardia lamblia/isolation & purification , Mamastrovirus/isolation & purification , Norovirus/isolation & purification , Acute Disease/epidemiology , Adenoviridae/pathogenicity , Adenoviridae Infections/diagnosis , Adenoviridae Infections/epidemiology , Astroviridae Infections/diagnosis , Astroviridae Infections/pathology , Caliciviridae Infections/diagnosis , Caliciviridae Infections/epidemiology , Child, Preschool , Diarrhea/epidemiology , Diarrhea/microbiology , Egypt/epidemiology , Feces/microbiology , Feces/parasitology , Feces/virology , Giardia lamblia/pathogenicity , Giardiasis/diagnosis , Giardiasis/epidemiology , Humans , Immunoenzyme Techniques , Infant , Mamastrovirus/pathogenicity , Norovirus/pathogenicity , Prevalence , Reagent Kits, Diagnostic , Seasons , Sensitivity and SpecificityABSTRACT
Stool samples from children < 5 years of age with diarrhea (N = 239) were examined for enteric pathogens using a combination of culture, enzyme-immunoassay, and polymerase chain reaction methods. Pathogens were detected in 122 (51%) stool samples; single pathogens were detected in 37.2% and co-pathogens in 13.8% of samples. Norovirus, rotavirus, and diarrheagenic Escherichia coli (DEC) were the most frequently detected pathogens (15.5%, 13.4%, and 11.2%, respectively); Salmonella, adenovirus, and Aeromonas were detected less frequently (7.9%, 7.1%, and 4.2%). The most commonly detected DEC was enteroaggregative E. coli (5.4%). Resistance to ≥ 3 antimicrobials was observed in 60% (18/30) of the bacterial pathogens. Salmonella resistance to ciprofloxacin (63.1%) has become a concern. Enteric viral pathogens were the most significant causative agents of childhood diarrhea in Tripoli. Bacterial pathogens were also important contributors to pediatric diarrhea. The emergence of ciprofloxacin-resistant Salmonella represents a serious health problem that must be addressed by Libyan health authorities.
Subject(s)
Anti-Infective Agents/therapeutic use , Ciprofloxacin/therapeutic use , Diarrhea/epidemiology , Diarrhea/microbiology , Aeromonas/drug effects , Aeromonas/isolation & purification , Aeromonas/pathogenicity , Child, Preschool , Drug Resistance, Bacterial , Escherichia coli/isolation & purification , Escherichia coli/pathogenicity , Feces/microbiology , Female , Humans , Infant , Infant, Newborn , Libya/epidemiology , Male , Norovirus/isolation & purification , Norovirus/pathogenicity , Prevalence , Rotavirus/isolation & purification , Rotavirus/pathogenicity , Salmonella/drug effects , Salmonella/isolation & purification , Salmonella/pathogenicityABSTRACT
Development of a genetic tool for the detection of genes encoding enterotoxins and colonization factors would greatly enhance enterotoxigenic Escherichia coli (ETEC) surveillance. Oligonucleotide primers were designed to amplify genes encoding human ST, porcine ST, LT and the structural genes of colonization factor antigen (CFA)/I, CS1 to CS8, CS12 to CS15, CS17 to CS22, and PCFO71. Screening 89 ETEC isolates phenotypically expressing a known CFA showed that, without exception, the multiplex polymerase chain reaction (mPCR) detected the structural gene of the expressed CFA, in addition to CS21 in 22.5% of isolates. Silent genes such as cssB (CS6) were also detected in 9.0%. Additionally, we screened 71 CFA phenotypically negative isolates and detected a CFA in more than 50% of tested isolates. In conclusion, we have designed a simple 4-step mPCR for the rapid detection of ETEC virulence factors. The assay is rapid, reproducible, relatively inexpensive, and has the potential to be field applicable.
Subject(s)
Enterotoxigenic Escherichia coli/genetics , Enterotoxigenic Escherichia coli/isolation & purification , Escherichia coli Infections/diagnosis , Fimbriae Proteins/genetics , Polymerase Chain Reaction/methods , Bacterial Toxins/genetics , DNA Primers/genetics , Enterotoxins/genetics , Escherichia coli Proteins/genetics , Humans , Polymerase Chain Reaction/economics , Reproducibility of Results , Time FactorsABSTRACT
The previous phase of the present study revealed that when crude extracts of Culex pipiens midgut, ovaries, and salivary glands are injected into New Zealand White (NZW) rabbits (Oryctolagus cuniculus), rabbits immunized with midgut extract exert the greatest negative impact on adult Cx. pipiens survival and fecundity. This study was conducted to further our understanding of the immunogenic nature of the aforementioned antigenic preparations, thus providing data for the ultimate goal of developing a vaccine against the numerous Cx. pipiens-vectored diseases that affect human populations throughout the world. Extracts of Cx. pipiens midgut, ovaries, and salivary glands were fractionated using Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE). The high (> 80.0 to >106.0 kDa) and low (< 18.5 kDa) molecular weight (MW) fractions of midgut extract, high MW fractions (75.0 - <106.0 kDa) of salivary gland extract, and low MW fraction (27.5 kDa) of ovary extract were excised, and used to immunize rabbits. Following immunization, anti-sera from all immunized rabbits were assayed for antibody response using Enzyme-Linked Immunosorbent Assay (ELISA), Enzyme Immunoblot transfer (EIB), and Indirect Fluorescent Antibody Techniques (IFAT). These assays resulted in both high and low MW fractions of midgut extract, with special reference to the midgut extract low MW fraction (18.0 kDa), eliciting the strongest humoral responses in immunized hosts. When Cx. pipiens were fed on rabbits immunized with the low MW fractions of midgut extract, the fecundity and survival rates were significantly less than those of mosquitoes fed on rabbits immunized with the high MW fractions of midgut extract and control rabbits (P < 0.001). It is concluded that, the low MW fraction of midgut extract is highly immunogenic, and the antibody response of immunized rabbits contributes to a significant disturbance in the life cycle of Cx. pipiens and their progeny. This impairment of feeding behavior and reproduction, in turn, could interfere with pathogen transmission.
Subject(s)
Animal Structures/chemistry , Antibodies/immunology , Antibodies/pharmacology , Culex/drug effects , Culex/physiology , Tissue Extracts/immunology , Administration, Oral , Animal Structures/immunology , Animals , Antibodies/administration & dosage , Antigens/immunology , Blotting, Western , Culex/chemistry , Enzyme-Linked Immunosorbent Assay , Feeding Methods , Female , Fluorescent Antibody Technique, Indirect , Gastrointestinal Tract/chemistry , Gastrointestinal Tract/immunology , Growth and Development/drug effects , Immunoglobulin G/blood , Immunoglobulin G/immunology , Insect Proteins/immunology , Life Cycle Stages/drug effects , Ovary/chemistry , Ovary/immunology , Rabbits , Reproduction/drug effects , Salivary Glands/chemistry , Salivary Glands/immunology , Survival Rate , Tissue Extracts/analysis , VaccinationABSTRACT
Insecticide use continues to be the primary control strategy to reduce insect vector populations. Concerns about insecticide resistance in target organisms, environmental degradation, and possible deleterious impact on human health have led researchers to seek a variety of alternative control strategies. We tested a relatively new method for controlling mosquitoes using host immune response. New Zealand White (NZW) rabbits (Oryctolagus cuniculus) were immunized with salivary gland (SGE), midgut (MGE), or ovary (OVE) extracts from female Culex pipiens L. Immunized rabbits were then exposed to unfed adult mosquitoes which were subsequently observed for changes in survival, fecundity, and hatch success. Parents that fed upon MGE- (P<0.001), SGE- (P<0.018) and OVE- (P<0.018) immunized rabbits experienced significantly higher mortality within 48 hours than parents fed on control rabbits. Midgut extract elicited the strongest effects upon survival (P<0.001), oviposition activity (P<0.001), and hatch success (P<0.001) in the parent generation. Survival (P<0.018), oviposition activity (P<0.001), and hatch success (P<0.001) were likewise strongly reduced in parents fed on SGE-immunized rabbits. Ovary extract-fed parents experienced less pronounced, but significant reductions, in survival (P<0.018) and hatch success (P<0.034). Surviving progeny were most strongly impacted by feeding upon MGE-immunized rabbits. Our study suggests that manipulating host immune response may be a suitable technique for reducing Cx. pipiens mosquito survival and fecundity, and subsequently the potential risk of disease transmission by this species.
Subject(s)
Antibodies/immunology , Antigens/immunology , Culex/immunology , Immunization , Animals , Antibody Formation , Culex/physiology , Female , Fertility , RabbitsABSTRACT
Enterotoxigenic Escherichia coli (ETEC) causes substantial diarrheal morbidity and mortality in young children in countries with limited resources. We determined the phenotypic profiles of 915 ETEC diarrheal isolates derived from Egyptian children under 3 years of age who participated in a 3-year population-based study. For each strain, we ascertained enterotoxin and colonization factor (CF) expression, the O:H serotype, and antimicrobial susceptibility. Sixty-one percent of the strains expressed heat-stable enterotoxin (ST) only, 26% expressed heat-labile enterotoxin (LT) alone, and 12% expressed both toxins. The most common CF phenotypes were colonization factor antigen I (CFA/I) (10%), coli surface antigen 6 (CS6) (9%), CS14 (6%), and CS1 plus CS3 (4%). Fifty-nine percent of the strains did not express any of the 12 CFs included in our test panel. Resistance of ETEC strains to ampicillin (63%), trimethoprim-sulfamethoxazole (52%), and tetracycline (43%) was common, while resistance to quinolone antibiotics was rarely detected. As for the distribution of observed serotypes, there was an unusually wide diversity of O antigens and H types represented among the 915 ETEC strains. The most commonly recognized composite ETEC phenotypes were ST CS14 O78:H18 (4%), ST (or LTST) CFA/I O128:H12 (3%), ST CS1+CS3 O6:H16 (2%), and ST CFA/I O153:H45 (1.5%). Temporal plots of diarrheal episodes associated with ETEC strains bearing common composite phenotypes were consistent with discrete community outbreaks either within a single or over successive warm seasons. These data suggest that a proportion of the disease that is endemic to young children in rural Egypt represents the confluence of small epidemics by clonally related ETEC strains that are transiently introduced or that persist in a community reservoir.