Immunolocalization of retinol-binding protein, cellular retinoic acid-binding protein I and retinoid X receptor beta in the porcine reproductive tract during the oestrous cycle.

Retinoid-binding proteins and nuclear receptors are expressed in the reproductive tissues of different species and their expression is hormonally regulated. In the present study, we demonstrated immunocytochemically the temporal and spatial localization of retinol-binding protein (RBP), cellular retinoic acid-binding protein I (CRABPI) and retinoid X receptor beta (RXRbeta) in porcine ovary, oviduct and uterus during the oestrous cycle. RBP and CRABPI were localized in the cytoplasm, whereas RXRbeta occurred in the nucleus. RBP was not detected in either the ovary or the oviduct at any stage of the oestrous cycle. CRABPI was present in luteal cells of the ovary only during dioestrus and in glandular and ciliated cells of the oviduct during oestrus. In the ovary, RXRbeta was always present in granulosa cells and germinal epithelium, with highest levels observed during oestrus. In the uterus, RXRbeta was present throughout the cycle in both the endometrium and the myometrium. However, changes in RXRbeta were observed in the endometrium, with highest levels observed during dioestrus. RBP and CRABPI could be observed in the endometrium only during dioestrus. The results show that the occurrence of retinoid-binding proteins and nuclear receptors in individual tissues of the reproductive tract are strongly dependent on the stage of the oestrous cycle. In the oviduct, the expression of CRABPI seems to be dependent on oestrogen, whereas in the uterus the expression of RBP and CRABPI is influenced by progesterone. The association of expression in different sections of the reproductive tissues investigated shows that the presence of specific proteins involved in retinoid metabolism was dependent on events associated with ovulation, the migration of the oocyte through the oviduct and the possible implantation of the blastocyst into the uterus.

[Simultaneous retroperitoneal operation of juxtarenal abdominal aortic aneurysm and ischemic vertebral body necrosis]. / Die einzeitige retroperitoneale Operation eines juxtarenalen Bauchaortenaneurysmas und einer ischämischen Wirbelkörpernekrose.

The combination of abdominal aortic aneurysm (AAA) and necrosis of the lumbar vertebral bodies is often the consequence of ischemia of the lumbar arteries and local compression from the aneurysm. A patient with necrosis of lumbar vertebral bodies 2 to 4 was admitted for abdominal aneurysm repair. CT scanning revealed almost complete destruction of the second and fourth lumbar vertebral bodies. In a combined operation an orthopedic and a vascular surgical team implanted two carbonic cages with autogenous splinter of the pelvic bone and an aortic vascular graft, using a retroperitoneal approach. Three months after the operation the 61-year-old man is entirely well and without any signs of back pain. He could be fully mobilized within 3 weeks postoperatively. This case study depicts the surgical techniques and discusses the advantages of the simultaneous operation and retroperitoneal exposure.

[Spondylitis caused by malignant duodeno-retroperitoneal histiocytosis--a case report]. / Spondylitis bedingt durch eine maligne duodeno-retroperitoneale Histiozytose--eine Kasuistik.

It is reported about a very extensive spondylitis of the 3rd to 5th lumbar vertebra body. The result of the autopsy was a "malign, duodenal histiocytosis". A "duodeno-retroperitoneal-fistula" led to the destruction of the vertebral bodies.

[Results of follow-up studies following proximal corrective osteotomy of the tibia]. / Nachuntersuchungsergebnisse nach proximaler Tibiakorrekturosteotomie.

Indication and method of proximal tibial corrective osteotomy in varus or valgus gonarthrosis are represented on the basis of a follow-up examination (after a period of 5.6 years on an average).

[Therapy of acromioclavicular luxation]. / Die Therapie der Akromioklavikularluxation.

62 patients underwent surgery for dislocation of the acromioclavicular joint and were followed up. In acute cases the best results could be achieved by an open reposition in combination with temporarily splinting by a Kirschner-wire and suture of capsula and ligament. A plasty using autologous ligament is necessary in rare cases only. Open reposition, screwing according to Bosworth and a plasty with a strip of autogenous cutis (Bunnell) should be performed in delayed cases.

[Fractures of the femur following prosthetic replacement of the hip-and knee joint (author's transl)]. / Femurfrakturen bei Totalendoprothesenplastiken.

10 patients who underwent prosthetic hip-or knee replacement suffered intra- and postoperatively from fractures of the femoral shaft. Loosened prostheses were replaced by longshafted prostheses. As long as the shaft of the prosthesis fits tight, stabilisation of the fracture by the plate can be achieved.

[Value of radiological diagnosis in the complex study of femur head necrosis in children. 1. Plain film and arthography in the framework of complex studies of femur head necrosis]. / Die Bedeutung der radiologischen Diagnostik bei komplexer Untersuchung von Femurkopfkernnekrosen im Wachstumsalter. 1. Mitteilung: Das Nativröntgenbild und die Arthrographie im Rahmen der komplexen Untersuchung bei Femurkopfkernnekrosen.

The results of complex investigations of case history, clinical methods, plain film radiology, laboratory findings, arthrography, histology, scintigraphy, and angiography give information of the picture of necrosis of the femoral head ossification center. Especially from the radiological diagnosis conclusions can be drawn for secondary prevention, diagnosis, prognosis, and therapy.

[Initial experiences with subcutaneous Harrington distraction without spondylodesis in scoliosis]. / Erste Erfahrungen mit der subkutanen Harrington-Distraktion ohne Spondylodese bei Skoliosen.

We report on a special method in the treatment of scoliosis using subcutaneous Harrington-distraction without spondylodesis. We operated 14 patients in all who were between 7 and 12 years old, in average 10 2/12 years Indication is made if scoliosis is extremely progressive, if Cobb's measure is above 50 degrees, after unsuccessful conservative treatment also including orthosis and physiotherapy, or if patients appeared to late in our department. In this technique only two incisions to make are necessary to place the Harrington roots to the cranial and caudal end of the scoliosis curve. The Harrington root can be 'needled' in subcutaneously. When in times of further growth the amount of correction gets noticeably lost the cranial hook could be tightened or the Harrington root could be changed easily. We need an orthosis (Cheneau or Milwaukee) for after treatment until in a final operation with spinal fusion is made. This method has showed good results although we had to cope with a bad prognostic starting position.

[Personal experiences using the Chêneau brace]. / Unsere Erfahrungen mit dem Chêneau-Korsett.

Idiopathic scoliosis under 20 degrees were treated by physiotherapeut methods alone. At scoliosis with curves between 20 and 45 degrees a correction by means of Chêneau type orthosis; is additionally used during growing age. The over all results of this combined therapy are good.

[A rare course of retroperitoneal malignant histiocytosis with duodeno-retroperitoneal fistula]. / Seltener Verlauf einer retroperitonealen malignen Histiozytose mit duodeno-retroperitonealer Fistelung.

A report is given on a 59-year-old female with retroperitoneal malignant histiocytosis and duodeno-retroperitoneal fistula. This had caused extensive autodigestion of the tumour and secondary inflammatory necrosis of the lumbar vertebral bodies. The uncommon finding and its rarity are discussed.

Distribution of vitamin A, retinol-binding protein, cellular retinoic acid-binding protein I, and retinoid X receptor beta in the porcine uterus during early gestation.

Retinol and retinol-binding protein (RBP), among the major secretory products of the uterine endometrium in the uterine fluid of pigs, are assumed to be of importance for early embryonic development. While uterine RBP has been widely characterized, little information is available on the metabolism of vitamin A itself or other specific binding proteins or nuclear receptors in the uterus of pigs. In the present study, the content and distribution of vitamin A in uterine tissue of pigs during early gestation (Days 14-30) were examined macroscopically and microscopically via autofluorescence and HPLC. In addition, the distribution of specific proteins involved in vitamin A metabolism at the cellular and nuclear level was investigated. Macroscopically, the yellowish-greenish autofluorescence characteristic of vitamin A was observed in uterine endometrium. Microscopy showed that the autofluorescence was associated with glandular and surface epithelium of the endometrium. In these structures, immunoreactive RBP was localized, as was cellular retinoic acid-binding protein I. Retinoid X receptor beta was observed in the nucleus of myometrium and endometrium. The intensity of fluorescence decreased with the progress of gestation. This decrease was paralleled by a decrease in vitamin A content of endometrium and myometrium. In general, vitamin A concentration in the endometrium was higher than in the myometrium (P < 0.01). In the myometrium, if present at all, vitamin A was found almost exclusively as retinyl esters. In the endometrium, the dominant fraction was retinol, representing more than 90% of total vitamin A. These results show for the first time that the yellowish-greenish autofluorescence in the pig uterus can be attributed to vitamin A. Differences in the form of vitamin A present in endometrium and myometrium might point to differences in metabolism. In the myometrium, vitamin A might be stored, and in the endometrium, vitamin A is present primarily as retinol-the form in which it is secreted into the uterine fluid.

[Value of radiological diagnosis in the complex study of femur head necrosis in children. 2. Scintigraphic and arteriographic studies in the framework of the complex diagnosis of femur head necrosis]. / Die Bedeutung der radiologischen Diagnostik bei komplexer Untersuchung von Femurkopfkernnekrosen im Wachstumsalter. 2. Mitteilung: Szintigraphische und arteriographische Untersuchungen im Rahmen der komplexen Diagnostik bei Femurkopfkernnekrosen.

101 scintigraphic investigations and 42 angiograms show that the vascular tamponade is the decisive factor for the beginning of necrosis of the femoral head ossification center. Plain films, scintigraphy and angiography give information of the duration of the disease. Extent and localisation of the circulatory disturbance are important for the prognosis of the disease. From stage classification and prognostic factors the conclusions for therapy can be drawn.