Dissociation of Mouse Olfactory Mucosae for Fluorescence-Activated Cell Sorting of Olfactory Sensory Neurons.

An increasing diversity of techniques investigating the biology of specific cell types and individual cells have elevated the importance of dissociation of viable cells from living tissues. Here we describe a method for the dissociation of single cells from samples of adult mouse olfactory mucosae, with an emphasis on maximizing yield of viable single cells from fluorescence-activated cell sorting. Yields are typically in the range of 80,000-150,000 viable cells per adult mouse.