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1.
Bioorg Chem ; 95: 103503, 2020 01.
Article in English | MEDLINE | ID: mdl-31855825

ABSTRACT

An investigation of a co-culture of the Armillaria sp. and endophytic fungus Epicoccum sp. YUD17002 associated with Gastrodia elata led to the isolation of eight new compounds, including five protoilludane-type sesquiterpenes (1-5) and three aryl esters (6-8), together with six known analogues (9-14). The assignments of their structures were conducted via extensive analyses of the spectroscopic data and comparison of experimental and calculatedelectronic circular dichroism(ECD)data. Notably, these new compounds were not present in the pure culture controls and were only detected in the co-cultures. Compound 4 is the first example of an ent-protoilludane sesquiterpenoid scaffold bearing a five-membered lactone. Compound 6 exhibited moderate in vitro cytotoxic activities against five human cancer cell lines (HL-60, A549, MCF-7, SMMC-7721, and SW480) with IC50 values ranging from 15.80 to 23.03 µM. Moreover, 6 showed weak acetylcholinesterase inhibitory activity (IC50 value of 23.85 µM).


Subject(s)
Antineoplastic Agents, Phytogenic/pharmacology , Armillaria/chemistry , Ascomycota/chemistry , Cholinesterase Inhibitors/pharmacology , Coculture Techniques , Gastrodia/chemistry , Polycyclic Sesquiterpenes/pharmacology , Acetylcholinesterase/metabolism , Antineoplastic Agents, Phytogenic/chemistry , Antineoplastic Agents, Phytogenic/isolation & purification , Cell Proliferation/drug effects , Cholinesterase Inhibitors/chemistry , Cholinesterase Inhibitors/isolation & purification , Dose-Response Relationship, Drug , Drug Screening Assays, Antitumor , Humans , Molecular Structure , Polycyclic Sesquiterpenes/chemistry , Polycyclic Sesquiterpenes/isolation & purification , Structure-Activity Relationship , Tumor Cells, Cultured
2.
J Nat Prod ; 82(4): 1009-1013, 2019 04 26.
Article in English | MEDLINE | ID: mdl-30785282

ABSTRACT

Co-culturing the endophytic fungus Phoma sp. YUD17001 from Gastrodia elata with Armillaria sp. in liquid nutrient medium resulted in the production of five new secondary metabolites, including two phenolic compounds, phexandiols A and B (1 and 2), three aliphatic ester derivatives, phomesters A-C (3-5), and a known fatty acid (6). The structures and absolute configurations of these compounds were elucidated by the interpretation of data from detailed spectroscopic analysis, Mosher's method, and electronic circular dichroism spectra, together with consideration of the biogenetic origins. None of the five new compounds were detected in single-strain cultures under identical fermentation conditions. The results of this work indicated that the production of 1-5 involved a complicated interaction process. None of the new compounds possessed significant cytotoxicity or antimicrobial activities.


Subject(s)
Armillaria/metabolism , Ascomycota/metabolism , Symbiosis , Circular Dichroism , Coculture Techniques , Fermentation
3.
Fitoterapia ; 140: 104422, 2020 Jan.
Article in English | MEDLINE | ID: mdl-31756377

ABSTRACT

Peniterester (1), a new tricyclic sesquiterpene, together with 6 known compounds (2-7) were isolated from the secondary metabolites of an artificial mutant Penicillium sp. T2-M20 which was obtained from the parental strain Penicillium sp. T2-8 via UV irradiation as well as nitrosoguanidine (NTG) induction. Peniterester was only produced by the mutant T2-M20 on the basis of LC-MS analysis. Meanwhile, the results of in vitro bioactivities screening indicated that peniterester owned obvious antibacterial activities against Bacillus subtilis, Escherichia coli and Staphylococcus aureus with MICs of 8.0, 8.0 and 4.0 µg/mL, respectively.


Subject(s)
Anti-Bacterial Agents/pharmacology , Penicillium/chemistry , Sesquiterpenes/pharmacology , Anti-Bacterial Agents/isolation & purification , Bacillus subtilis/drug effects , Cell Line, Tumor , China , Escherichia coli/drug effects , Gastrodia/microbiology , Humans , Microbial Sensitivity Tests , Molecular Structure , Rhizome/microbiology , Secondary Metabolism , Sesquiterpenes/isolation & purification , Staphylococcus aureus/drug effects
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