ABSTRACT
A tetrodotoxin- and hexamethonium-sensitive response to thyrotropin-releasing hormone (TRH), an in vitro contraction, first appeared in the duodenum of the 3 day old rat, was increased by day 16, decreased there-after and was extinguished after weaning. A tetrodotoxin-resistant response to TRH, a relaxation, appeared at day 13 and became distinct after weaning. The development of neurogenic receptivity for TRH precedes the appearance of myogenic receptivity in the rat duodenum.
Subject(s)
Aging , Animal Population Groups/physiology , Animals, Newborn/physiology , Animals, Suckling/physiology , Muscle, Smooth/physiology , Thyrotropin-Releasing Hormone/pharmacology , Acetylcholine/pharmacology , Animals , Duodenum/physiology , Female , Hexamethonium Compounds/pharmacology , In Vitro Techniques , Male , Muscle Contraction , Rats , Scopolamine/pharmacology , Tetrodotoxin/pharmacology , Verapamil/pharmacologyABSTRACT
Following three series of electric footshocks (10 shocks/day), one out of three rats in most cages were brought to emit ultrasonic vocalization for several minutes after a single shock. The characteristics of shock-elicited ultrasound were pure tone pulses of a frequency between 22 and 28 kHz, with duration longer than 300 msec. The same type of ultrasound is produced by subordinate male rats during agonistic behavior. The intracerebroventricular injection of beta-endorphin, dynorphin, methionine-enkephalin or leucine-enkephalin attenuated the shock-elicited ultrasonic vocalization. Psychotropic drugs such as diazepam and chlorpromazine also attenuated the shock-elicited ultrasonic vocalization. A test utilizing ultrasonic vocalization in rodents can provide useful data for studying the psychotropic properties of neuropeptides.
Subject(s)
Endorphins/pharmacology , Fear/drug effects , Vocalization, Animal/drug effects , Animals , Dynorphins/pharmacology , Enkephalins/pharmacology , Injections, Intraventricular , Male , Psychotropic Drugs/pharmacology , Rats , Rats, Inbred Strains , Substance P/pharmacology , beta-EndorphinABSTRACT
An intracerebroventricular (i.c.v.) injection of TRH to the urethane anesthetized rat stimulates the activity of the superior laryngeal nerve (n.sl) which is a vagal ramus terminating at the thyroid gland and adjacent muscles. The response to TRH, a tonic increase in the n.sl outflow, was dose dependent in the 0.005-5.0 micrograms/100 g B.W. range. In contrast to this, methionine-enkephalin (ENK), neurotensin (NT) and somatostatin (SRIF) (5 micrograms/100 g, i.c.v.) all caused a transient decrease in n.sl activity. SRIF showed the highest attenuating effect when injected alone and was capable of diminishing the increased activity produced by a prior injection of TRH. ENK and NT failed to affect the TRH-induced increased activity. When injected concomitantly with TRH, SRIF blocked the response to TRH while ENK and NT both failed to affect the response to TRH. Pretreatment with triiodothyronine for 5 days strongly inhibited the response of the n.sl outflow to TRH. On the other hand, pretreatment with atropine, haloperidol, propranolol, phenoxybenzamine and p-chlorophenylalanine failed to block the stimulating effect of TRH although the response was diminished by some antagonists. It therefore seemed that TRH transmission is involved in central stimulation and SRIF is antagonistic in this regulation of n.sl outflow to the thyroid gland.
Subject(s)
Laryngeal Nerves/physiology , Thyroid Gland/physiology , Thyrotropin-Releasing Hormone/pharmacology , Animals , Dose-Response Relationship, Drug , Enkephalin, Methionine , Enkephalins/pharmacology , Laryngeal Nerves/drug effects , Male , Neurotensin/pharmacology , Rats , Rats, Inbred Strains , Somatostatin/pharmacology , Thyrotropin-Releasing Hormone/antagonists & inhibitors , Triiodothyronine/pharmacologyABSTRACT
The nerve activity of the gastric ramus of the splanchnic (sympathetic) nerve, gastric ramus of the vagus, adrenal ramus of the splanchnic nerve and the superior laryngeal nerve (laryngeal ramus of vagus) were assessed before and after i.c.v. injection of neuropeptides in the rat. TRH stimulated the vagal branch but attenuated the sympathetic outflow to the stomach. In contrast, the sympathetic outflow to the adrenal was enhanced by TRH. SRIF suppressed the activity of all the nerves studied. VIP did not affect the sympathetic outflow to the stomach while suppressing the gastric branch of the vagus. The adrenal sympathetic branch as well as the superior laryngeal nerve was stimulated by VIP. Bombesin suppressed both vagal and sympathetic outflow to the stomach but markedly stimulated the laryngeal branch of the vagus. The adrenal sympathetic nerve was either stimulated or attenuated slightly by bombesin. These results indicate that centrally administered neuropeptides produce reactions specific for each nerve.
Subject(s)
Autonomic Nervous System/drug effects , Nerve Tissue Proteins/pharmacology , Adrenal Glands/innervation , Animals , Autonomic Nervous System/physiology , Bombesin/pharmacology , Male , Nerve Tissue Proteins/physiology , Rats , Rats, Inbred Strains , Somatostatin/pharmacology , Stomach/innervation , Thyrotropin-Releasing Hormone/pharmacology , Vasoactive Intestinal Peptide/pharmacologyABSTRACT
The intracerebroventricular (i.c.v.) injection of rabbit antiserum to thyrotropin-releasing hormone (TRH) to the urethane anesthetized rat inhibited the spontaneous electrical discharge of the superior laryngeal nerve (n.sl). On the other hand, the i.c.v. injection of rabbit antiserum to somatostatin (SRIF) failed to influence the nerve activity whereas SRIF itself is capable of inhibiting the n.sl activity. These findings suggest that TRH in the brain takes a role continuously in regulating the neural activity while SRIF is involved in the neuronal circuits as an agent for the down regulation of the autonomic nervous system.
Subject(s)
Efferent Pathways/physiology , Immune Sera , Thyroid Gland/innervation , Thyrotropin-Releasing Hormone/pharmacology , Vagus Nerve/physiology , Animals , Efferent Pathways/drug effects , Laryngeal Nerves/drug effects , Laryngeal Nerves/physiology , Male , Rats , Rats, Inbred Strains , Somatostatin/immunology , Somatostatin/pharmacology , Thyrotropin-Releasing Hormone/immunology , Vagus Nerve/drug effectsABSTRACT
The mode of inhibitory action of centrally administered SRIF on the efferent activity of autonomic nerves was investigated in the rat by assessing the SRIF-induced change in the activity of the superior laryngeal nerve with or without pretreatment with various drugs. After picrotoxin or bicuculline treatment, the inhibition of nerve activity by SRIF was abolished while reserpine and atropine failed to abolish the SRIF effect. The centrally administered GABA inhibited the activity of the superior laryngeal nerve and the cervical sympathetic trunk. However, SRIF did not affect the sympathetic trunk. Arterial blood pressure was increased by SRIF while GABA produced hypotension. These data provide evidence for a GABAergic system as the mediator of SRIF action in the brain and for the selectivity of SRIF action on the particular intermediary GABAergic neurones.
Subject(s)
Autonomic Nervous System/physiology , Somatostatin/physiology , gamma-Aminobutyric Acid/physiology , Animals , Atropine/pharmacology , Bicuculline/pharmacology , Blood Pressure/drug effects , Ganglia, Sympathetic/physiology , Hemodynamics/drug effects , Injections, Intraventricular , Laryngeal Nerves/physiology , Male , Picrotoxin/pharmacology , Rats , Rats, Inbred Strains , Reserpine/pharmacology , Somatostatin/pharmacology , gamma-Aminobutyric Acid/pharmacologyABSTRACT
Ontogenic changes in ultrasound production by isolated rat pups were compared in male and female pups of litters of various sex compositions. In both sexes, the peak of emission of sound around 50 kHz was on days 4-5, while 40 kHz sound production peaked on days 6-7. By days 4-5 each pulse is an inverted V-shape with frequencies between 40 and 55 kHz. At the peak of sound production, pulses with a slight downsweep from 40-45 kHz were prominent, and in the preweaning period, pulses of a relatively stable frequency of 35-40 kHz were emitted. There was a general tendency for sound pulses of female pups to be of short duration compared with those of male pups. The ontogenic changes in the ultrasonic distress call are less variable throughout all female pups of litters consisting of 2, 4, 6 or 8 female pups in a litter of 8. In contrast to this, it was found that male pups emit ultrasound more vigorously than female pups from days 2-3 to days 12-13 when male and female pups coexisted in the same litter. The activity of sound production of male pups which had no female littermates was similar to that of female pups. These findings suggest that the interaction of some kind between male and female pups produces the sex differences in the ultrasonic distress calls as early as several days after birth in the rat.
Subject(s)
Sex Characteristics , Vocalization, Animal/physiology , Aging/physiology , Animals , Animals, Newborn , Body Weight , Female , Male , Rats , Rats, Inbred Strains , Sound Spectrography , UltrasonicsABSTRACT
The evoked responses to 100-ms tone signals of 8, 22, 50 or 70 kHz were recorded from the frontal cortex in the conscious female rat throughout the estrous cycle. The stimulus-bound evoked potentials did not vary with the estrous cycle. At estrus, 50 kHz sounds produced a negative potential peaking at 200 ms in the cortex. In other combinations of sound frequency and estrous stage no late potentials were detected. This finding demonstrates the presence of human 'Event-related potential'-like endogenous potentials in the rat that responds to ultrasonic signals which mimic those used in intraspecific communication.
Subject(s)
Arousal/physiology , Auditory Perception/physiology , Dominance, Cerebral/physiology , Electroencephalography , Estrus/physiology , Animals , Auditory Pathways/physiology , Evoked Potentials, Auditory , Female , Inferior Colliculi/physiology , Rats , Rats, Inbred Strains , Sexual Behavior, Animal/physiology , UltrasonicsABSTRACT
Movement-concurrent rhythmic slow activity (RSA) was recorded by means of 3 pairs of bipolar electrodes which were arranged so that they orthogonally encompassed the dorsal hippocampus in the freely moving rat. The 3-channel data were combined by synchronously plotting them on independent voltage axes and the resultant 3-channel Lissajous' trajectory (3-CLT) was depicted in 3-dimensional microcomputer processing. A type of 3-CLT which traversed the surface of an ellipsoid with the long axis nearly perpendicular to the medial CA1 pyramidal layer, appeared most abundantly during walking. A 3-CLT depicting curves on a spherical surface was frequently produced during sitting. A linear 3-CLT of short duration was occasionally produced during rearing. These findings suggest that alteration of voltage axis specific to a behavior is possibly generated associating with the hippocampal RSA.
Subject(s)
Behavior, Animal/physiology , Electroencephalography , Hippocampus/physiology , Animals , Brain Stem/physiology , Electrodes , Hippocampus/cytology , Male , Neurons/physiology , Pyramidal Tracts/physiology , Rats , Rats, Inbred StrainsABSTRACT
The effects of centrally administered thyrotropin-releasing hormone (TRH) on vagal efferent and cortical electroencephalographic (EEG) activity were assessed in rat brains which were functionally isolated from the body trunk and maintained via cross-circulation. Upon withdrawal of inputs from the trunk, cervical vagal activity was markedly attenuated. The decreased activity was partially restored by TRH with a latent period of a few minutes. On the other hand, TRH immediately but transiently augmented the fast waves of the EEG. The change in brain activity produced by TRH appears to simulate the state which makes possible the generation of vagal efferent activity even though inputs from the body trunk are withdrawn.
Subject(s)
Cerebral Cortex/drug effects , Electroencephalography , Thyrotropin-Releasing Hormone/pharmacology , Vagus Nerve/drug effects , Animals , Cross Circulation , Female , Injections, Intraventricular , Rats , Rats, Inbred StrainsABSTRACT
Electroenteromyographic activity (EMG) of the duodenum was recorded in pentobarbital-anesthetized rats. TRH intraventricularly administered to rats produced changes in EMG such as increased amplitude, decreased frequency of slow waves and the association of bursts of spike potentials with nearly every cycle of the basal electric rhythm (BER). The effect was selectively prompt and marked in the EMG of proximal duodenum. The response was abolished by vagotomy or atropine injection and no response was elicited in the neonatally 6-OHDA-treated rat. Hypophysectomy, cord-transection or acute i.v. injection of 6-OHDA did not block the response. In the brain, TRH seems to stimulate the neuronal system controlling the vagus efferents involved in the regulation of the duodenal enteric nervous system which in turn modulates the myogenic excitability of the duodenum.
Subject(s)
Muscle, Smooth/drug effects , Thyrotropin-Releasing Hormone/pharmacology , Animals , Duodenum/drug effects , Electromyography , Female , Injections, Intraventricular , Male , Rats , Thyrotropin-Releasing Hormone/administration & dosageABSTRACT
The study concerned the postnatal ontogenesis of the response to methionine5-enkephalin (ENK) in the duodenum of developing rats. Sustained relaxation was produced by ENK in the isolated duodenum of 8 day old rats. The response was blocked by tetrodotoxin or naloxone. The response increased by day 18, decreased thereafter and was extinguished after 40 days. On the other hand, a tetrodotoxin resistant response to ENK, transient relaxation, appeared at day 20 and was augmented thereafter. The development of neurogenic receptivity for ENK preceded the appearance of myogenic receptivity in the rat duodenum.
Subject(s)
Duodenum/metabolism , Enkephalin, Methionine/metabolism , Muscle, Smooth/metabolism , Aging , Animals , Animals, Newborn/metabolism , Duodenum/innervation , In Vitro Techniques , Morphine/metabolism , Muscle Relaxation/drug effects , Norepinephrine/pharmacology , Rats , Rats, Inbred StrainsABSTRACT
A neonatal cerebral cortical lesion was made in rats and the effects of diazepam on ultrasonic isolation calls in pups and footshock-elicited ultrasonic distress calls in young adult rats were assessed. There was no indication that the cortical lesion influenced the production of the ultrasonic distress calls in either pups or adults. Diazepam attenuated the ultrasonic isolation calls in all the pups with and without cortical lesion, and the distress calls in normal adult rats. However, diazepam failed to exert the effect in rats which received a neonatal cortical lesion. 8-Hydroxy-2-(di-n-propylamino)tetralin hydrobromide (8-OH-DPAT), another anxiolytic, was effective to diminish the distress calls even in the adult rats which had had the neonatal damage to the cortex. These findings indicate that the intact cerebral cortex is not always required for production of ultrasonic distress calls; however, the development of the neuronal mechanism involving benzodiazepine receptors to inhibit the ultrasonic expression of anxiety or fear in adult rats is dependent on the integrity of the cerebral cortex.
Subject(s)
Anxiety/physiopathology , Cerebral Cortex/physiology , Diazepam/pharmacology , 8-Hydroxy-2-(di-n-propylamino)tetralin/pharmacology , Animals , Animals, Newborn , Female , Male , Rats , Rats, Wistar , Receptors, GABA-A/physiology , Receptors, Serotonin/physiology , Ultrasonics , Vocalization, Animal/drug effectsABSTRACT
Ultrasonic distress calls elicited in male rats by footshock under inescapable conditions were suppressed by diazepam. The suppression was blocked by pretreatment with Ro15-1788 or CGS8216, selective benzodiazepine receptor antagonists, while an opiate antagonist naloxone failed to modify the effect of diazepam. In contrast, naloxone antagonized but Ro15-1788 and CGS8216 did not antagonize beta-endorphin which also suppressed the ultrasonic vocalization. These findings suggest that benzodiazepine and opioid receptors may contribute separately to antianxiety activity.
Subject(s)
Diazepam/pharmacology , Endorphins/pharmacology , Vocalization, Animal/drug effects , Animals , Convulsants/pharmacology , Depression, Chemical , Flumazenil/pharmacology , Injections, Subcutaneous , Male , Pyrazoles/pharmacology , Rats , Rats, Inbred Strains , UltrasonicsABSTRACT
Thyrotropin-releasing hormone (TRH) produced a contraction in the isolated segment of duodenum and taenia coli of the guinea-pig (pA2, 8.0 and 8.9). TRH induced a contraction, a relaxation, or a contraction followed by relaxation in the jejunum and ileum. All the responses to TRH of the small intestine and the taenia coli were abolished in the presence of tetrodotoxin but not affected by hexamethonium. The contractile response to TRH of the small intestine was abolished and replaced by a relaxation in the presence of hyoscine. This relaxant response was not affected by guanethidine. The taenial response to TRH was partially inhibited by either hyoscine or methysergide and markedly diminished by the two together. These findings indicate that TRH acts on the myenteric neurons of the small intestine and taenia coli of the guinea pig. The contractile response of small intestine is likely to be induced through cholinergic nerves while cholinergic, serotonergic and unidentified excitatory neurons seem to be involved in the taenial response. These neurogenic actions of TRH on the guinea-pig intestine are in contrast with the myogenic natur of the response to TRH in the duodenum of the rat.
Subject(s)
Intestines/drug effects , Muscle Contraction/drug effects , Thyrotropin-Releasing Hormone/pharmacology , Animals , Dose-Response Relationship, Drug , Guanethidine/pharmacology , Guinea Pigs , Hexamethonium Compounds/pharmacology , In Vitro Techniques , Indomethacin/pharmacology , Intestine, Small/drug effects , Intestine, Small/physiology , Intestines/innervation , Intestines/physiology , Male , Methysergide/pharmacology , Muscle Relaxation/drug effects , Myenteric Plexus/drug effects , Physostigmine/pharmacology , Scopolamine/pharmacology , Serotonin/pharmacology , Somatostatin/pharmacology , Tetrodotoxin/pharmacology , Thyrotropin-Releasing Hormone/antagonists & inhibitorsABSTRACT
Met-enkephalin (ENK) induced a dose-dependent transient relaxation in vitro in the rat duodenum while morphine neither induced a response nor affected the response to ENK. The response was not blocked by tetrodotoxin but was abolished by naloxone. The response of guinea-pig duodenum to ENK was either relaxation or pulsatile contractions depending on the dose and was abolished by tetrodotoxin.
Subject(s)
Endorphins/pharmacology , Enkephalins/pharmacology , Muscle Contraction/drug effects , Muscle Relaxation/drug effects , Muscle, Smooth/drug effects , Animals , Drug Interactions , Duodenum/drug effects , Enkephalin, Methionine , Guinea Pigs , In Vitro Techniques , Male , Morphine/pharmacology , Rats , Species Specificity , Tetrodotoxin/pharmacologyABSTRACT
Neonatal chickens were injected intraventricularly with Ni(II), Pd(II), Cu(II) or Zn(II) complex of TRH and the potencies of stimulating locomotor activity were compared with that of TRH, Ni(II)-TRH was more potent than the ligand while Pd(II)-TRH was inert. Cu(II)- and Zn(II)-TRH induced the response to the same extent of the ligand. These results indicate that the action of TRH in the CNS resulting in the locomotor hyperactivity is dependent on the tertiary conformation of molecule which is modified by chelate formation.