Microalgae biofilm cultured in nutrient-rich water as a tool for the phycoremediation of petroleum-contaminated water.

This study aimed at studying the phycoremediation of petroleum-contaminated water using microalgae biofilm cultured in nutrient-rich water. Microalgae biofilm was grown in a photobioreactor containing water rich in calcium nitrate, manganese chloride, sodium potassium tartrate, calcium phosphate, and ammonium sulfate. Petroleum contaminated water was poured into a photobioreactor, and the substrate containing microalgae biofilm was inserted into the photobioreactor and allowed for eight weeks for biofilm formation. Physicochemical parameters (pH, turbidity, conductivity, sulfate, alkalinity, chloride, TDS, TSS, nitrate, salinity, iron, potassium, phosphate, chlorine, chromium, magnesium, zinc, COD, BOD, and total petroleum hydrocarbon (TPH) of the petroleum contaminated water before and after treatment were determined. The microalgae biofilm used for the treatment was characterized before and after treatment using a Scanning Electron Microscope, X-Ray Fluorescence, and Fourier-transform infrared spectroscopy. The phytochemical constituent of the microalgae biofilm was also determined before and after treatment of the petroleum-contaminated water. The result obtained shows highest removal efficiency of physicochemical parameters (turbidity (81%), conductivity (51.2), sulfate (17.5%), alkalinity 28.4%), chloride (14.6%), TDS (7.9), TSS (26%), nitrate (33%), salinity (23.4), iron (16%), potassium (22%), phosphate (28.2%), chlorine (14%), chromium (13.6%), magnesium (30.3%), zinc (40.5%), COD (8%), BOD (16.7%) and total petroleum hydrocarbon (15%)). The microalgae's characterization shows microalgae biofilm's ability to adsorb pollutants in petroleum-contaminated water due to the presence of microspores and larger surface area of the cells of the microalgae forming the biofilm or due to the absorption efficiency of the extracellular polymeric substances (EPS). The analysis of the microalgae biofilm's phytochemical parameters shows the involvement of the chemicals components in pollutants degradation and antioxidant response of the microalgae to counteract the oxidative effect resulting from the exposure of the microalgae to the contaminated water. NOVELTY STATEMENT This is the first study that attempts the phycoremediation of petroleum contaminated water using microalgae biofilm. The reduction efficiency of the parameters treated in this study is very low compared to that reported in the literature but increases with the retention day. This low reduction efficiency is attributed to the slow assimilation of organic and inorganic pollutants due to the initial growth condition. This study is the first to re-affirm that microalgae biofilm can phycoremediate petroleum-contaminated water by adsorption and assimilation due to the presence of microspores and a larger surface area the cells of the microalgae forming the biofilm or the extracellular polymetric surface covering the biofilm. Several studies have reported that phytochemicals present in microalgae play an antioxidant response role to prevent the microalgae from oxidative damage resulting from water pollution. However, this study is the first to strongly link phytochemicals to the enhancement of pollutants degradation and adsorption by microalgae biofilm.

Evaluation of Pistia stratiotes fractions as effective larvicide against Anopheles mosquitoes.

Mosquito are well-known vectors that cause diseases particularly malaria and filariasis which are detrimental to human health. These vectors occur mainly in tropical countries where more than 2 billion people live in endemic regions with about one million deaths been claimed yearly from malaria and filariasis. The study is aimed at evaluating the larvicidal activity of Pistia stratiotes fractions on Anopheles mosquitoes (Diptera: Culicidae). The ethyl acetate extract of P. stratiotes was obtained through percolation process and was chromatographed to yield nine fractions. The larvicidal activity of each of the nine fractions was tested in triplicates by exposing the larvae to 500, 250, 125, 62.5 and 31.3 µg/ml, respectively. Phytochemical screening of the nine fractions revealed the presence of alkaloids, flavonoids, glycosides and phlobatannins in varying quantities. The result obtained shows that fraction E has the highest lethal effect on the Anopheles larvae at LC50 =14.81 µg/ml and was weakly effective at 602.03 µg/ml on brine shrimp larvae. The gas chromatography mass spectrometry analysis of fraction E revealed the presence of 35 pre-cursor compounds. Hence, ethyl acetate fractions of P. stratiotes could be an effective larvicide against Anopheles mosquito larvae as it has been found to be harmless to other aquatic organisms. Further work should be done on other aquatic weeds that have larvicidal potential to isolate the bioactive compounds.