Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 1 de 1
Filter
Add more filters

Database
Language
Affiliation country
Publication year range
1.
Yao Xue Xue Bao ; 48(7): 1169-77, 2013 Jul.
Article in Zh | MEDLINE | ID: mdl-24133987

ABSTRACT

The gene expressions of codeinone reductase (COR) and berberine bridge enzyme (BBE) in Papaver somniferum were blocked by RNA hairpin of RNA interference (RNAi). The complete sequences of COR and BBE genes were cloned by reverse transcription-polymerase chain reaction (RT-PCR), the results of homology comparison revealed that the cloned COR and BBE genes had high homology with the other gene family members reported in the GenBank. The target sequences of COR and BBE genes were screened in accordance with the design principle of RNAi, a 643 bp fusion gene was obtained by the method of overlapping PCR, then plant expression vector ihpRNA was constructed based on intermediate vector pHANNIBAL and plant expression vector pCEPSPS. With that 78 transgenic plants were obtained through Agrobacterium-mediated and 17 positive plants were screened by PCR, that could initially indicate that the target fragments of COR and BBE gene had been integrated into tobacco genome.


Subject(s)
NAD (+) and NADP (+) Dependent Alcohol Oxidoreductases/genetics , Nicotiana/genetics , Oxidoreductases, N-Demethylating/genetics , Papaver/enzymology , Papaver/genetics , Artificial Gene Fusion , Genetic Vectors , Plants, Genetically Modified/enzymology , Plants, Genetically Modified/genetics , RNA Interference , RNA, Small Interfering , Transformation, Genetic
SELECTION OF CITATIONS
SEARCH DETAIL