ABSTRACT
Recently, the incidence of thyroid cancer is increasing worldwide. Papillary thyroid cancer (PTC) is the most common histological type of thyroid cancer. Whole-transcriptome sequence analysis was performed to further understand the primary molecular mechanisms of the occurrence and progression of PTC. Results showed that Eva-1 homolog A (EVA1A) may be a potential gene for the PTC-associated gene in thyroid cancer. In this work, the role of EVA1A expression in thyroid cancer was investigated. Real-time PCR was performed to detect the expression level of EVA1A in 43 pairs of PTC and four thyroid cancer cell lines. The Cancer Genome Atlas (TCGA) database was used to evaluate the relationship between the expression level of EVA1A and the pathological feature of PTC. The logistic regression analysis of the TCGA data set indicated that the expression of EVA1A was an independent risk factor for tumour, nde and metastasis (TNM) in PTC. This study shows the down-regulation of EVA1A inhibited the colony formation, proliferation, migration and invasion of PTC cell lines. In the protein level, knockdown of EVA1A can regulate the expression of N-cadherin, vimentin, Bcl-xL, Bax, YAP and TAZ. This study indicated that EVA1A was an oncogene associated with PTC.
Subject(s)
Membrane Proteins/metabolism , Protein Serine-Threonine Kinases , Signal Transduction , Thyroid Cancer, Papillary/metabolism , Thyroid Neoplasms/metabolism , Adaptor Proteins, Signal Transducing/metabolism , Adult , Aged , Antigens, CD/metabolism , Apoptosis , Cadherins/metabolism , Cell Line, Tumor , Cell Movement , Cell Proliferation , Epithelial-Mesenchymal Transition/genetics , Female , Gene Expression Regulation, Neoplastic , Hippo Signaling Pathway , Humans , Intracellular Signaling Peptides and Proteins/metabolism , Male , Middle Aged , Neoplasm Invasiveness , Protein Serine-Threonine Kinases/metabolism , Risk Factors , Software , Thyroid Cancer, Papillary/pathology , Thyroid Neoplasms/pathology , Transcription Factors/metabolism , Transcriptional Coactivator with PDZ-Binding Motif Proteins , Vimentin/metabolism , YAP-Signaling Proteins , bcl-2-Associated X Protein/metabolism , bcl-X Protein/metabolismABSTRACT
Aim: Thyroid cancer (TC) is one of the most common types of endocrine malignancy and poses a significant challenge to human health. The long noncoding RNA 389641 (LOC389641) has been found to be associated with many types of cancer. However, the function of LOC389641 in papillary TC (PTC) remains unknown. Our aim is to explore LOC389641 expression and its role in TC. Materials & methods: The function of LOC389641 was determined by colony formation, migration and invasion assays in PTC. Western blot assays were performed to determine the biomarker of epithelial-mesenchymal transition. Results: In this study, we show that LOC389641 is involved in PTC, which suggests that it may be a target for TC therapies.