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Rheumatoid arthritis (RA) is the most prevalent inflammatory joint disease worldwide, leading to irreversible disability and even mortality. Unfortunately, current treatment regimens fail to cure RA due to low therapeutic responses and off-target side effects. Herein, a neutrophil membrane-cloaked, natural anti-arthritic agent leonurine (Leo), and catalase (CAT) co-loaded nanoliposomal system (Leo@CAT@NM-Lipo) is constructed to remodel the hostile microenvironment for RA remission. Due to the inflammation tropism inherited from neutrophils, Leo@CAT@NM-Lipo can target and accumulate in the inflamed joint cavity where high-level ROS can be catalyzed into oxygen by CAT to simultaneously accelerate the drug release and alleviate hypoxia at the lesion site. Besides, the neutrophil membrane camouflaging also enhances the anti-inflammatory potentials of Leo@CAT@NM-Lipo by robustly absorbing pro-arthritogenic cytokines and chemokines. Consequently, Leo@CAT@NM-Lipo successfully alleviated paw swelling, reduced arthritis score, mitigated bone and cartilage damage, and reversed multiple organ dysfunctions in adjuvant-induced arthritis rats (AIA) rats by synergistic effects of macrophage polarization, inflammation resolution, ROS scavenging, and hypoxia relief. Furthermore, Leo@CAT@NM-Lipo manifested excellent biocompatibility both at the cellular and animal levels. Taken together, the study provided a neutrophil-mimetic and ROS responsive nanoplatform for targeted RA therapy and represented a promising paradigm for the treatment of a variety of inflammation-dominated diseases.
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Recently high doping of lanthanide ions (till 100 %) is realized unprecedentedly in nanostructured upconversion (UC) phosphors. However, oddly enough, this significant breakthrough did not result in a corresponding UC enhancement at ambient temperature, which hinders the otherwise very interesting applications of these materials in various fields. In this work, taking the Er3+ -rich UC nanosystem as an example, we confirm unambiguously that the phonon-assisted cross relaxation (CR) is the culprit. More importantly, combining the theoretical modeling and experiments, the precise roles of different CR channels on UC energy loss are quantitatively revealed. As a result, lowering the temperature can exponentially enhance the relevant UC luminescence by more than two orders of magnitude. Our comprehension will play an important role in promoting the UC performance and further application of high doping rare earth materials. As a proof of concept, an Er3+ -rich core/multi-shell nanophosphor is exploited which demonstrates the great potential of our finding in the field of ultra-sensitive temperature sensing.
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Relatively low efficiency is the bottleneck for the application of lanthanide-doped upconversion nanoparticles (UCNPs). The high-level doping strategy realized in recent years has not improved the efficiency as much as expected. It is argued that cross relaxation (CR) is not detrimental to upconversion. Here we combine theoretical simulation and spectroscopy to elucidate the role of CR in upconversion process of Er3+ highly doped (HD) UCNPs. It is found that if CR is purposively suppressed, upconversion efficiency can be significantly improved. Specifically, we demonstrate experimentally that inhibition of CR by introducing cryogenic environment (40â K) enhances upconversion emission by more than two orders of magnitude. This work not only elucidates the nature of CR and its non-negligible adverse effects, but also provides a new perspective for improving upconversion efficiency. The result can be directly applied to cryogenic imaging and wide range temperature sensing.
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Simultaneous photothermal ablation of multiple tumors is limited by unpredictable photo-induced apoptosis, caused by individual intratumoral differences. Here, a multi-channel lanthanide nanocomposite was used to achieve tailored synergistic treatment of multiple subcutaneous orthotopic tumors under non-uniform whole-body infrared irradiation prescription. The nanocomposite reduces intratumoral glutathione by simultaneously activating the fluorescence and photothermal channels. The fluorescence provides individual information on different tumors, allowing customized prescriptions to be made. This enables optimal induction of hyperthermia and dosage of chemo drugs, to ensure treatment efficacy, while avoiding overtherapy. With an accessional therapeutic laser system, customized synergistic treatment of subcutaneous orthotopic cancer cases with multiple tumors is possible with both high efficacy and minimized side effects.
Subject(s)
Antineoplastic Agents , Hyperthermia, Induced , Nanocomposites , Nanoparticles , Neoplasms , Humans , Phototherapy , Antineoplastic Agents/pharmacology , Antineoplastic Agents/therapeutic use , Neoplasms/drug therapy , Nanocomposites/therapeutic use , Doxorubicin/pharmacology , Cell Line, TumorABSTRACT
OBJECTIVES: Even today, tuberculosis (TB) remains a leading public health problem; yet, the current diagnostic methods still have a few shortcomings. Lipoarabinomannan (LAM) provides an opportunity for TB diagnosis, and urine LAM detection seems to have a promising and widely applicable prospect. DESIGN OR METHODS: Four databases were systematically searched for eligible studies, and the quality of the studies was evaluated using the quality assessment of diagnostic accuracy studies-2 (QUADAS-2). Graphs and tables were created to show sensitivity, specificity, likelihood ratios, diagnostic odds ratio (DOR), the area under the curve (AUC), and so on. RESULTS: Based on the included 67 studies, the pooled sensitivity of urine LAM was 48% and specificity was 89%. In the subgroup analyses, the FujiLAM test had higher sensitivity (69%) and specificity (92%). Furthermore, among patients infected with human immunodeficiency virus (HIV), 50% of TB patients were diagnosed using a urine LAM test. Besides, the CD4+ cell count was inversely proportional to the sensitivity. CONCLUSIONS: Urine LAM is a promising diagnostic test for TB, particularly using the FujiLAM in HIV-infected adults whose CD4+ cell count is ≤100 per µl. Besides, the urine LAM test shows various sensitivities and specificities in different subgroups in terms of age, HIV infection status, CD4+ cell count, and testing method.
Subject(s)
Lipopolysaccharides/urine , Tuberculosis/diagnosis , Adult , CD4 Lymphocyte Count , Child , HIV Infections/complications , Humans , Mycobacterium tuberculosis , Sensitivity and Specificity , Tuberculosis/complications , Tuberculosis/urineABSTRACT
A copper-catalyzed efficient regioselective silylation reaction of chloro-substituted allenyl-Bdan was developed. Under mild reaction conditions, allenyl and propargyl silane compounds can be selectively obtained in moderate to high yields by adjusting the bases and solvents used in the reactions. This study offers direct and efficient methods for synthesizing multifunctionalized allenyl and propargyl silane compounds from the same initial material of chloro-substituted allenyl-Bdan.
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Since the 20th century, global climate has been recognized as the most important environmental factor affecting the distribution of plants. Angelica dahurica (A. dahurica) has been in great demand as a medicinal herb and flavoring, but the lack of seed sources has hindered its development. In this study, we utilized the Maxent model combined with Geographic Information System (GIS) to predict the potential habitat of A. dahurica in China based on its geographical distribution and 22 environmental factors. This prediction will serve as a valuable reference for the utilization and conservation of A. dahurica resources.The results indicated that: (1) the Maxent model exhibited high accuracy in predicting the potential habitat area of A. dahurica, with a mean value of the area under the ROC curve (AUC) at 0.879 and a TSS value above 0.6; (2) The five environmental variables with significant effects were bio6 (Min temperature of the coldest month), bio12 (Annual Precipitation), bio17 (Precipitation of Driest Quarter), elevation, and slope, contributing to a cumulative total of 89.6%. Suitable habitats for A. dahurica were identified in provinces such as Yunnan, Guizhou, Guangxi, Sichuan, Hunan, and others. The total area of suitable habitat was projected to increase, with expansion primarily in middle and high latitudes, while areas of decrease were concentrated in lower latitudes. Under future climate change scenarios, the centers of mass of suitable areas for A. dahurica were predicted to shift towards higher latitudes in the 2050s and 2090s, particularly towards the North China Plain and Northeast Plain. Overall, it holds great significance to utilize the Maxent model to predict the development and utilization of A. dahurica germplasm resources in the context of climate change.
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Avena sterilis L. (A. sterilis) and Avena ludoviciana Dur. (A. ludoviciana) are extremely invasive weeds with strong competitive ability and multiple transmission routes. Both species can invade a variety of dryland crops, including wheat, corn, and beans. Asia, as the world's major food-producing continent, will experience significant losses to agricultural production if it is invaded by these weeds on a large scale. This study used the MaxEnt model and ArcGIS to map the distribution of suitable habitats of the two species in Asia under climate change conditions. The constructed model comprised four levels, with a total of 25 index-level indicator factors used to evaluate the invasion risk of the two species. The results showed that the distribution of suitable habitats for both Avena species was highly dependent on precipitation and temperature. Under climate warming conditions, although overall the total suitable area is predicted to decrease compared to the current period, there are still moderately or highly suitable areas. Asian countries need to provide early warning for areas with significant increases in moderate and highly suitable zones for these two species of weeds under the background of climate change. If there is already an invaded area or if the suitability of the original area is increased, this should be closely monitored, and control measures should be taken to prevent further spread and deterioration.
Subject(s)
Avena , Climate Change , Introduced Species , Plant Weeds , Asia , Ecosystem , Crops, AgriculturalABSTRACT
Lactate is not only the energy substrate of neural cells, but also an important signal molecule in brain. In modern societies, disturbed circadian rhythms pose a global challenge. Therefore, exploring the influence of circadian period on lactate and its metabolic kinetics is essential for the advancement of neuroscientific research. In the present study, the different groups of mice (L: 8:00 a.m.; D: 20:00 p.m.; SD: 20:00 p.m. with 12 h acute sleep deprivation) were infused with [3-13C] lactate through the lateral tail vein for a duration of 2 min. After 30-min lactate metabolism, the animals were euthanized and the tissues of brain and liver were obtained and extracted, and then, the [1H-13C] NMR technology was employed to investigate the kinetic information of lactate metabolism in different brain regions and liver to detect the enrichment of various metabolic kinetic information. Results revealed the fluctuating lactate concentrations in the brain throughout the day, with lower levels during light periods and higher levels during dark periods. Most metabolites displayed strong sensitivity to circadian rhythm, exhibiting significant day-night variations. Conversely, only a few metabolites showed changes after acute sleep deprivation, primarily in the temporal brain region. Interestingly, in contrast to brain lactate metabolism, liver lactate metabolism exhibited a significant increase following acute sleep deprivation. This study explored the kinetics of lactate metabolism, hinted at potential clinical implications for disorders involving circadian rhythm disturbances, and providing a new research basis for clinical exploration of brain and liver lactate metabolism.
Subject(s)
Brain , Circadian Rhythm , Lactic Acid , Liver , Animals , Circadian Rhythm/physiology , Lactic Acid/metabolism , Kinetics , Male , Brain/metabolism , Liver/metabolism , Mice , Mice, Inbred C57BL , Magnetic Resonance Spectroscopy/methods , Sleep Deprivation/metabolismABSTRACT
Chionanthus retusus (C. retusus) has a high economic and medicinal value, but in recent years it has been included in the list of China's major protected plants and China's Red List of Biodiversity due to the serious destruction of its wild germplasm resources. Based on 131 sample points of C. retusus, this study simulated potential habitats and spatial changes of C. retusus in the 21st century using the Maxent model combined with the geographic information system ArcGIS, predicted prioritized protected areas by the Marxan model, and assessed current conservation status through GAP analysis. The results showed that (1) when the regularization multiplier was 1.5 and the feature combinations were linear, quadratic, and fragmented, the area under the curve of the subjects in the training and test sets were both above 0.9, the true skill statistic value was 0.80, and the maximum Kappa value was 0.62, meaning that the model had high accuracy; (2) Temperature seasonality, annual precipitation, min temperature for coldest month, and precipitation of wettest month had relatively strong influences on species' ranges. (3) The moderately and optimally suitable habitats of C. retusus were primly located in the areas of southwestern Shanxi, central Hebei, western Henan, Shandong, Shaanxi, Anhui and Hubei; (4) Under different future climate scenarios, the area of each class of suitable habitat will increase for varied amounts compared to the current period, with a general trend of expansion to the south; (5) The C. retusus priority protected areas were mainly located in most of Shandong, southern Liaoning, southwestern Shanxi, western Henan, and central Hebei, and its conservation vacancy area was relatively large compared to its protected area. These results will provide scientific strategies for implementing long-term conservation of C. retusus in China and similar regions under warming conditions in the 21st century.
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Introduction: Tilia amurensis Rupr (T. amurensis) is one endangered and national class II key protected wild plant in China. It has ornamental, material, economic, edible and medicinal values. At present, the resources of T. amurensis are decreasing, and the prediction of the distribution of its potential habitat in China can provide a theoretical basis for the cultivation and rational management of this species. Methods: In this study, the R language was used to evaluate 358 distribution records and 38 environment variables. The MaxEnt model was used to predict the potential distribution areas of T. amurensis under the current and future climate scenarios. The dominant environmental factors affecting the distribution of T. amurensis were analyzed and the Marxan model was used to plan the priority protected areas of this species. Results: The results showed that Bio18, Slope, Elev, Bio1, Bio9 and Bio2 were the dominant environmental factors affecting the distribution of T. amurensis. Under the future climatic scenarios, the potential suitable areas for T. amurensis will mainly distribute in the Northeast China, the total suitable area will reduce compared with the current climate scenarios, and the general trend of the centroid of suitable habitat will be towards higher latitudes. The SPF value of the best plan obtained from the priority conservation area planning was 1.1, the BLM value was 127,616, and the priority conservation area was about 57.61×104 km2. The results suggested that climate, soil and topographic factors jointly affected the potential geographical distribution of T. amurensis, and climate and topographic factors had greater influence than soil factors. Discussion: The total suitable area of T. amurensis in China under different climate scenarios in the future will decrease, so more effective protection should be actively adopted.
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There was an error in the original publication [...].
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ETHNOPHARMACOLOGICAL RELEVANCE: Insect tea is a special non-Camellia tea produced from the feces of insects in ethnic minority regions in Southwest China. Insect tea was recorded to have the traditional functions to treat summer heat, dampness, digestive disturbance, excessive phlegm, shortness of breath and otitis, etc. AIM OF THIS STUDY: This review provides an updated and comprehensive review of insect tea on the tea types, host plants, tea-producing insects, producing process, traditional uses, nutritional values, chemistry, pharmacological activities, biological safety and toxicity. Moreover, the general challenges and the possible suggestions on insect tea in future were also discussed. MATERIALS AND METHODS: The relevant literature on insect tea was obtained from scientific database, including Elsevier, PubMed, Springer, Wiley, Web of Science, Google Scholar, SciFinder, China National Knowledge Infrastructure (CNKI), Baidu Scholar, Wanfang Database, etc. In addition, pertinent information available in Ph.D theses, MSc. dissertations, books, records, and some classical Chinese herbal literature were also included. The references cited in this review were up to September 2022. RESULTS: Insect tea has been traditionally used as popular beverage with various medicinal functions in the ethnic minority regions in Southwest China for centuries. At present, ten types of insect teas are recorded in different regions. Ten species tea-producing insect and fifteen species host plants are used to in the production of the tea. Insect teas were rich in various nutrients including proteins, carbohydrates, fat, minerals, dietary fiber and Vitamins. A total of 71 compounds have been isolated from insect teas, mainly including flavonoids, ellagitannins, chlorogenic acids and other phenolic compounds as well as the alkaloids. Modern studies demonstrated that insect tea had a variety of pharmacological activities in vitro and in vivo, such as anti-diabetic, lipid-lowing and anti-hypertensive, hepato-protective, gastro-intestinal promoting, anti-cancer, anti-mutagenic, antioxidant, and anti-aging activity, etc. Moreover, existing experimental results showed that insect teas are non-toxicity and biological safety. CONCLUSION: Insect tea is a unique and niche product originated in the ethnic minority regions of Southwest China with various health-promoting benefits. Phenolics including flavonoids, ellagitannins, chlorogenic acids were reported as the main chemical constituents identified from insect tea. Multiple pharmacological activities of insect tea have been reported, which indicated that it had great potential for further development and application as drugs and health-promoting products. However, more studies on the tea-producing insects, host plants, chemistry and pharmacological activity of insect tea, as well as the toxicology should be further investigated.
Subject(s)
Ethnic and Racial Minorities , Ethnicity , Humans , Ethnopharmacology , Hydrolyzable Tannins , Minority Groups , China , Flavonoids , Nutrients , Tea , Phytochemicals/pharmacology , Medicine, Chinese Traditional/methodsABSTRACT
(1) SAH induces cellular stress and endoplasmic reticulum stress, activating the unfolded protein response (UPR) in nerve cells. IRE1 (inositol-requiring enzyme 1) is a protein that plays a critical role in cellular stress response. Its final product, Xbp1s, is essential for adapting to changes in the external environment. This process helps maintain proper cellular function in response to various stressors. O-GlcNAcylation, a means of protein modification, has been found to be involved in SAH pathophysiology. SAH can increase the acute O-GlcNAcylation level of nerve cells, which enhances the stress capacity of nerve cells. The GFAT1 enzyme regulates the level of O-GlcNAc modification in cells, which could be a potential target for neuroprotection in SAH. Investigating the IRE1/XBP1s/GFAT1 axis could offer a promising avenue for future research. (2) Methods: SAH was induced using a suture to perforate an artery in mice. HT22 cells with Xbp1 loss- and gain-of-function in neurons were generated. Thiamet-G was used to increase O-GlcNAcylation; (3) Results: Severe neuroinflammation caused by subarachnoid hemorrhage leads to extensive endoplasmic reticulum stress of nerve cells. Xbp1s, the final product of unfolded proteins induced by endoplasmic reticulum stress, can induce the expression of the hexosamine pathway rate limiting enzyme GFAT1, increase the level of O-GlcNAc modification of cells, and have a protective effect on neural cells; (4) Conclusions: The correlation between Xbp1s displayed by immunohistochemistry and O-GlcNAc modification suggests that the IRE1/XBP1 branch of unfolded protein reaction plays a key role in subarachnoid hemorrhage. IRE1/XBP1 branch is a new idea to regulate protein glycosylation modification, and provides a promising strategy for clinical perioperative prevention and treatment of subarachnoid hemorrhage.
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Soft robots possess unique deformability and hence result in great adaptability to various unconstructive environments; meanwhile, untethered soft actuation techniques are critical in fully exploiting their potential for practical applications. However, restricted by the material's softness and structural compliance, most untethered actuation systems were incapable of achieving fully soft construction with a powerful output. While in Nature, with a fully soft body, a squid can burst high-pressure jet flow from a cavity that drives the squid to swim swiftly. Here, inspired by such a unique actuation strategy of squids, an entirely soft pump capable of high-pressure output, fast jetting, and untethered control is presented, and it helps a bionic soft robotic squid to achieve a high-efficient untethered motion in water. The soft pump is designed by a reversible liquid-gas phase transition of an inductive heating magnetic liquid metal composite that acts as an adjustable power source with high heat efficiency. In particular, being purely soft, the pump can yet lift â¼20 times its weight and achieve â¼3 times the specific pressure of the previous record. It may promote the application of soft robots with independent actuation, high output power, and embodied energy supply.
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In the original publication [...].
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Rapid laboratory detection is remarkably crucial to diagnosing coronavirus disease 2019 (COVID-19) infection, due to whose outbreak causes to the world pandemic. The BinaxNOW antigen card (BinaxNOW) is a simple, effective, and cheap tool to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The meta-analysis in this study was conducted to evaluate the diagnostic performance of BinaxNOW for SARS-CoV-2. The researchers independently retrieved the related databases (PubMed, Embase, Web of Science, Cochrane Library) before May 1st, 2021, and extracted the relevant data based on the early inclusion/exclusion criterion. Quality Assessment of Diagnostic Accuracy Study-2 was used to evaluate the quality of the enrolled studies. Stata 16.0, Meta-DiSc 1.4, and Review Manager 5.3 were used to generate analytical data for the statistical analysis. 59 sets of data were identified from the seven studies included in this meta-analysis. The combined sensitivity, specificity, positive likelihood ratio, negative likelihood ratio, diagnostic odds ratio, and their 95% confidence intervals were 0.77 (0.76 to 0.79), 0.99 (0.99 to 0.99), 65.72 (48.23 to 89.56), 0.23 (0.19 to 0.28), and 461.10 (281.55 to 755.13), respectively. The area under curve was 0.9910 in the summary receiver operating characteristic curve. BinaxNOW is beneficial for symptomatic patients' onset within 7 days. CT value and testing site may be the heterogeneity source of BinaxNOW accuracy. Moreover, this technology has an efficient performance for diagnosing COVID-19, especially in patients with heavy viral load. BinaxNOW may become a practical tool for large-scale or at-home use for COVID-19 in the post-pandemic era.Highlightsâ Pooled sensitivity with 0.77 and specificity with 0.99 in the BinaxNOW assay.â CT value and testing site may be the heterogeneity source of BinaxNOW accuracy.â BinaxNOW is beneficial for symptomatic patients' onset within 7 days.â BinaxNOW may become a practical tool for large-scale or at-home use for COVID-19.
Subject(s)
COVID-19 , SARS-CoV-2 , Humans , Databases, Factual , Odds Ratio , Pandemics , COVID-19 TestingABSTRACT
When combined with diverse sensors, soft robots significantly improve their functionalities and intelligence levels. However, most of the existing soft sensors require complex signal analysis devices or algorithms, which severely increase the complexity of soft robot systems. Here, based on the unique fluidic property of liquid metal, we propose a magnet-controllable soft microswitch that can be well-integrated into a soft robot system, e.g., a soft gripper to help it facilely detect and precisely grab objects. The microswitch consists of a flexible soft beam electrode and a fixed electrode, forming a soft microsystem. By tuning the cohesion force of the liquid metal between the electrodes, the microswitch can convert its states between an individual and a self-locking state. The microswitch can achieve a reasonable rapid response (~12 ms) and high switching frequency (~95 Hz). Furthermore, soft microswitches can be customized into logic units and also coupled to control a digital tube showing various numbers. Our work provides a new simple soft sensor unit that may enhance the intelligence of soft systems.
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A copper-catalyzed borylation of propargyl dichlorides was developed under mild reaction conditions. The corresponding chloro-substituted allenyl-Bdan products were obtained in good yields. The utilities of allenyl-Bdan products were examined by their diverse derivatizations.
Subject(s)
Copper , Catalysis , StereoisomerismABSTRACT
Here, we describe the synthesis of a novel type of rare-earth-doped nanoparticles (NPs) for multimodal imaging, by combining the rare-earth elements Ce, Gd and Nd in a crystalline host lattice consisting of CaF2 (CaF2: Ce, Gd, Nd). CaF2: Ce, Gd, Nd NPs are small (15-20 nm), of uniform shape and size distribution, and show good biocompatibility and low immunogenicity in vitro. In addition, CaF2: Ce, Gd, Nd NPs possess excellent optical properties. CaF2: Ce, Gd, Nd NPs produce downconversion emissions in the second near-infrared window (NIR-II, 1000-1700 nm) under 808 nm excitation, with a strong emission peak at 1056 nm. Excitation in the first near- infrared window (NIR-I, 700-900 nm) has the advantage of deeper tissue penetration power and reduced autofluorescence, compared to visible light. Thus, CaF2: Ce, Gd, Nd NPs are ideally suited for in vivo fluorescence imaging. In addition, the presence of Gd3+ makes the NPs intrinsically monitorable by magnetic resonance imaging (MRI). Moreover, next to fluorescence and MR imaging, our results show that CaF2: Ce, Gd, Nd NPs can be used as imaging probes for photoacoustic imaging (PAI) in vitro. Therefore, due to their biocompatibility and suitability as multimodal imaging probes, CaF2: Ce, Gd, Nd NPs exhibit great potential as a traceable imaging agent in biomedical applications.