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Development and validation of a sensitive and specific sodB-based quantitative PCR assay for molecular detection of Ehrlichia species.

Qurollo, Barbara A; Riggins, Dana; Comyn, Alaire; Zewde, Merone T; Breitschwerdt, Edward B.

J Clin Microbiol ; 52(11): 4030-2, 2014 Nov.

Article in English | MEDLINE | ID: mdl-25143580

ABSTRACT

We developed a sensitive and specific sodB-based quantitative PCR assay to detect Ehrlichia spp. The assay's limit of detection was 5 copies/reaction, and it did not amplify nonspecific DNA. Compared with a 16S rRNA gene PCR target, the sodB target may offer an improved molecular diagnostic assay to detect Ehrlichia spp.

Subject(s)

Bacterial Proteins/genetics , Ehrlichia/genetics , Ehrlichia/isolation & purification , Ehrlichiosis/diagnosis , Real-Time Polymerase Chain Reaction/methods , Superoxide Dismutase/genetics , Animals , DNA, Bacterial/chemistry , DNA, Bacterial/genetics , Dogs , Ehrlichiosis/microbiology , Ehrlichiosis/veterinary , Humans , Molecular Sequence Data , RNA, Ribosomal, 16S/genetics , Sensitivity and Specificity , Sequence Analysis, DNA

ABSTRACT

Subject(s)

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