Multiple fluorescence polymer dots-based differential array sensors for highly efficient heavy metal ions detection.

Water pollution caused by harmful heavy metal ions (HMIs) can significantly impact aquatic ecosystems and pose a high risk to human health. In this work, equipped with ultra-high fluorescence brightness, efficient energy transfer, and environmentally friendly performance, polymer dots (Pdots) were employed to construct a pattern recognition fluorescent HMIs detection platform. A single-channel unary Pdots differential sensing array was first developed to identify multiple HMIs with 100% classification accuracy. Then an "all-in-one" multiple Förster resonance energy transfer (FRET) Pdots differential sensing platform was constructed to discriminate HMIs in the artificial polluted water samples and actual water samples, exhibiting high classification accuracy in distinguishing HMIs. The proposed strategy leverages the compounded cumulative differential variation of diverse sensing channels for analytes, which is anticipated to find extensive applications in other fields for detection purposes.

Microfluidic-based platforms for cell-to-cell communication studies.

Intercellular communication is critical to the understanding of human health and disease progression. However, compared to traditional methods with inefficient analysis, microfluidic co-culture technologies developed for cell-cell communication research can reliably analyze crucial biological processes, such as cell signaling, and monitor dynamic intercellular interactions under reproducible physiological cell co-culture conditions. Moreover, microfluidic-based technologies can achieve precise spatial control of two cell types at the single-cell level with high throughput. Herein, this review focuses on recent advances in microfluidic-based 2D and 3D devices developed to confine two or more heterogeneous cells in the study of intercellular communication and decipher the advantages and limitations of these models in specific cellular research scenarios. This review will stimulate the development of more functionalized microfluidic platforms for biomedical research, inspiring broader interests across various disciplines to better comprehend cell-cell communication and other fields, such as tumor heterogeneity and drug screening.

Functionalized graphene-based electrochemical array sensors for the identification of distinct conformational states of Amyloid Beta in Alzheimer's disease.

Aß oligomers have been widely accepted as significant biomarkers for Alzheimer's disease (AD) detection, monitoring, and therapy since they are highly correlated with AD development. In this work, an electrochemical array-based sensing platform was successfully built using a group of functionalized graphene with different physicochemical features. Since the electro-insulated Aß peptide species severely interfered with the electron transport on the electrode surface, the presence of Aß led to a significant change in the electrochemical impedance signal. The resulting variety of the impedance was then classified and processed by linear discriminant analysis. The constructed sensing platform can discriminate different Aß forms, the mixture of various Aß forms, and different ratios of Aß42 to Aß40 with 100% accuracy by only the combination of dual probes. Furthermore, it also exhibited excellent performance for screening Aß inhibitors and metal chelators. The strategy utilizes the infinitesimal general discrepancy instead of specific biomarker recognition, exhibiting the advantage of no requirement to know the exact information about the specific ligand and receptor in advance, which is promising to be widened for the other biosensing detection fields.