ABSTRACT
Antianxiety action of diterpene alkaloid songorine was studied using Vogel conflict test. Songorine in a dose of 0.25 mg/kg demonstrated high anxiolytic activity comparable to that of phenazepam and produced no sedative effect.
Subject(s)
Alkaloids/pharmacology , Anti-Anxiety Agents/pharmacology , Diterpenes/pharmacology , Stress, Psychological/drug therapy , Aconitum/chemistry , Alkaloids/isolation & purification , Animals , Anti-Anxiety Agents/isolation & purification , Behavior, Animal/drug effects , Benzodiazepines/pharmacology , Conflict, Psychological , Diterpenes/isolation & purification , Dose-Response Relationship, Drug , Male , Mice , Mice, Inbred CBA , Stress, Psychological/physiopathologyABSTRACT
This study demonstrated the possibility of in vivo activation of progenitor cells by hyaluronidase. Specifically, treatment with hyaluronidase increased the number of mesenchymal and bone marrow precursor cells, their proliferative activity, and differentiation. Also, it promoted stem cell mobilization into blood under effect of granulocyte colony-stimulating factor (G-CSF) and enhanced progenitor cell adhesive properties. Therapeutic efficiency of transplantation of mononuclear cells isolated from peripheral blood after administration of G-CSF improved under effect of hyaluronidase. Hyaluronidase immobilized by electron beam synthesis nanotechnology exhibited high specific activity with respect to stem cells via both enteral and parenteral routes.