ABSTRACT
In rats with modeled posttraumatic knee osteoarthrosis, negative changes in subchondral bone metabolism were revealed: a tendency to an increase in osteocalcin concentration, a decrease in sclerostin and osteoprotegerin levels, and a significant increase in FGF-23 concentration accompanied by a slight elevation of inorganic phosphorous and significant increase in total calcium levels in comparison with the corresponding parameters in intact controls. These findings demonstrate crucial importance of structural integrity of the subchondral bone, because its protection improves the results of reconstructive therapy for local cartilage defects.
Subject(s)
Bone and Bones/metabolism , Knee Injuries/complications , Osteoarthritis, Knee/etiology , Animals , Animals, Outbred Strains , Bone and Bones/pathology , Calcium/metabolism , Cartilage, Articular/metabolism , Disease Models, Animal , Knee Injuries/metabolism , Knee Injuries/pathology , Knee Joint/metabolism , Knee Joint/pathology , Male , Osteoarthritis, Knee/metabolism , Osteoarthritis, Knee/pathology , Osteocalcin/metabolism , Osteoprotegerin/metabolism , Phosphorus/metabolism , RatsABSTRACT
Using the rat model of posttraumatic osteoarthrosis of the knee joint induced by surgical transection of their anterior cruciate ligaments, we showed that irreversible loss of hyaluronan by the extracellular matrix of the joint cartilage tissue against the background of oxidative stress accompanied by accumulation of intermediate LPO products in blood serum and formation of thiol system incompetence was one of the key patterns of dystrophic degeneration of the cartilage tissue. Considerable metabolic shifts were associated with structural modification of the articular hyaline cartilage: its thinning and a decrease of chondrocyte density and their abnormal spatial distribution in the matrix with predominance of solitary isolated cells with signs of karyopyknosis and karyolysis.