ABSTRACT
To confirm circulation of Anajatuba virus in Maranhao, Brazil, we conducted a serologic survey (immunoglobulin G ELISA) and phylogenetic studies (nucleocapsid gene sequences) of hantaviruses from wild rodents and persons with hantavirus pulmonary syndrome. This virus is transmitted by Oligoryzomys fornesi rodents and is responsible for hantavirus pulmonary syndrome in this region.
Subject(s)
Disease Reservoirs/virology , Environmental Monitoring , Hantavirus Pulmonary Syndrome/epidemiology , Orthohantavirus/classification , Sigmodontinae/virology , Adult , Animals , Antibodies, Viral/blood , Brazil/epidemiology , Contact Tracing , Cross-Sectional Studies , Epidemiological Monitoring , Female , Orthohantavirus/genetics , Orthohantavirus/isolation & purification , Hantavirus Pulmonary Syndrome/blood , Hantavirus Pulmonary Syndrome/veterinary , Humans , Immunoglobulin G/blood , Male , Molecular Sequence Data , Phylogeny , RNA, Viral/analysis , RNA, Viral/genetics , Seroepidemiologic StudiesABSTRACT
A cross-sectional serosurvey was conducted to assess the proportion of persons exposed to hantaviruses in a virus-endemic area of the state of Minas Gerais, Brazil. Findings of this study suggested the presence of > or =1 hantaviruses circulating in this region causing hantavirus pulmonary syndrome, mild disease, or asymptomatic infection.
Subject(s)
Hantavirus Pulmonary Syndrome/epidemiology , Adolescent , Adult , Aged , Antibodies, Viral/blood , Brazil/epidemiology , Child , Cross-Sectional Studies , Enzyme-Linked Immunosorbent Assay , Female , Humans , Immunoglobulin G/blood , Male , Middle AgedABSTRACT
Pasteurised bovine milk from retail markets in the State of São Paulo, Brazil, was analysed for the presence of streptomycin (STP) and dihydrostreptomycin (DHSTP) residues. An ELISA kit was used for screening and a LC-APCI-MS/MS QToF method for confirmatory analysis. Both methods were intra-laboratory validated and found suitable for screening and confirmatory testing, respectively, for STP and DHSTP residues in pasteurised bovine milk at concentration levels below the maximum residue limit (MRL) established for these substances (200 µg kg(-1) expressed as the sum of the concentrations of STP and DHSTP). No residues of STP and DHSTP at detectable levels were found in the analysed samples (n = 299).