Genome analysis of Shewanella putrefaciens 4H revealing the potential mechanisms for the chromium remediation.

Microbial remediation of heavy metal polluted environment is ecofriendly and cost effective. Therefore, in the present study, Shewanella putrefaciens stain 4H was previously isolated by our group from the activated sludge of secondary sedimentation tank in a dyeing wastewater treatment plant. The bacterium was able to reduce chromate effectively. The strains showed significant ability to reduce Cr(VI) in the pH range of 8.0 to 10.0 (optimum pH 9.0) and 25-42 ℃ (optimum 30 ℃) and were able to reduce 300 mg/L of Cr(VI) in 72 h under parthenogenetic anaerobic conditions. In this paper, the complete genome sequence was obtained by Nanopore sequencing technology and analyzed chromium metabolism-related genes by comparative genomics The genomic sequence of S. putrefaciens 4H has a length of 4,631,110 bp with a G + C content of 44.66% and contains 4015 protein-coding genes and 3223,  2414, 2343 genes were correspondingly annotated into the COG, KEGG, and GO databases. The qRT-PCR analysis showed that the expression of chrA, mtrC, and undA genes was up-regulated under Cr(VI) stress. This study explores the Chromium Metabolism-Related Genes of S. putrefaciens 4H and will help to deepen our understanding of the mechanisms of Cr(VI) tolerance and reduction in this strain, thus contributing to the better application of S. putrefaciens 4H in the field of remediation of chromium-contaminated environments.

Effect of Size Tuning of Hexagonal SnS2 Nanosheet on the Efficiency of Photocatalytic Degradation Processes.

Recent research on SnS2 materials aims to enhance their photocatalytic efficiency for water pollution remediation through doping and constructing heterojunctions. These methods face challenges in cost-effectiveness and practical scalability. This study synthesizes hexagonal SnS2 nanosheets of various sizes via a hydrothermal method, assessing their performance in degrading methyl orange (MO) and reducing hexavalent chromium (Cr(VI)). The results show that smaller SnS2 nanosheets exhibit higher photocatalytic efficiency under sunlight. Specifically, 50 mg of small-sized nanosheets degraded 100 ml of MO (10 mgL-1) in 30 min and reduced Cr(VI) (10 mgL-1) in 105 min. The enhanced performance is attributed to: i) an energy bandgap of 2.17 eV suitable for visible light, and ii) more surface sulfur (S) vacancies in smaller nanosheets, which create electronic states near the Fermi level, reducing electron-hole recombination. This study offers a straightforward strategy for improving 2D materials like SnS2.

Sustainable Biomass Acts as an Electron Donor for Cr(VI) Reduction during the Subcritical Hydrothermal Process: Molecular Insights into the Role of Hydrochar and Liquid Compounds.

Heavy metal pollution is a critical environmental issue that has garnered significant attention from the international community. Subcritical hydrothermal liquefaction (HTL) as an emerging green technology has demonstrated remarkable promise in environmental remediation. However, there is limited research on the remediation of highly toxic Cr(VI) using HTL. This study reveals that the HTL reaction of biomass enables the simultaneous reduction and precipitation of Cr(VI). At 280 °C, the reduction of Cr(VI) was nearly complete, with a high reduction rate of 98.9%. The reduced Cr as Cr(OH)3 and Cr2O3 was primarily enriched in hydrochar, accounting for over 99.9% of the total amount. This effective enrichment resulted in the removal of Cr(VI) from the aqueous phase while simultaneously yielding clean liquid compounds like organic acids and furfural. Furthermore, the elevated temperature facilitated the formation of Cr(III) and enhanced its accumulation within hydrochar. Notably, the resulting hydrochar and small oxygenated compounds, especially aldehyde, served as electron donors for Cr(VI) reduction. Additionally, the dissolved Cr facilitated the depolymerization and deoxygenation processes of macromolecular compounds with lignin-like structures, leading to more small oxygenated compounds and subsequently influencing Cr(VI) reduction. These findings have substantial implications for green and sustainable development.

New insight into the effects of p-benzoquinone on photocatalytic reduction of Cr(VI) over Fe-doped g-C3N4.

It is of great significance to establish an effective method for removing Cr(VI) from wastewater. Herein, Fe-doped g-C3N4 (namely Fe-g-C3N4-2) was synthesized and then employed as photocatalyst to conduct the test of Cr(VI) reduction. Notably, the embedding of Fe ion in g-C3N4 can offer the Fe2+/Fe3+ redox couples, so reducing the interfacial resistance of charge transfer and suppressing the recombination of photogenerated electrons and holes. The impurity energy levels will form in g-C3N4 after the introduction of Fe ion, thereby boosting the light absorption capacity of catalyst. Thus, Fe-g-C3N4-2 showed good performance in photocatalytic Cr(VI) reduction, and the reduction efficiency of Cr(VI) can reach 39.9% within 40 min. Different with many previous studies, current work unexpectedly found that the addition of p-benzoquinone (BQ) can promote the Cr(VI) reduction, and the reduction efficiency of Cr(VI) over Fe-g-C3N4-2 was as high as 93.2% in the presence of BQ (1.5 mM). Further analyses showed that BQ can be reduced to hydroquinone (HQ) by photogenerated electrons, and UV light can also directly induce BQ to generate HQ by using H2O as the hydrogen donor. The HQ with reducing ability can accelerate the Cr(VI) reduction. In short, current work shared some novel insights into photocatalytic Cr(VI) reduction in the presence of BQ. Future research should consider possible reactions between photogenerated electrons and BQ. For the UV-induced photocatalysis, the suitability of BQ as the scavenger of O2•‒ must be given carefully consideration.

Preparation of core-shell structure Ag@TiO2 plasma photocatalysts and reduction of Cr(VI): Size dependent and LSPR effect.

The poor light absorption and low carrier separation efficiency of Titanium dioxide (TiO2) limit its further application. The introduction of plasma metal Ag have the potential to solve these drawbacks owing to its plasma resonance effect. Thus core-shell structure Ag@TiO2 plasma photocatalysts was prepared by using facile reduction method in this work. More specifically, Ag@TiO2 composite catalysts with different Ag loading amounts were prepared in the presence of surfactant PVP. Ag@TiO2 demonstrates excellent light absorption performance and photoelectric separation efficiency compared with pure TiO2. As a result, it displays excellent performance of Cr(VI) reduction under visible light. The optimal composite catalysts Ag@TiO2-5P achieves exceptional visible-light-driven photocatalytic Cr(VI) reduction efficiency of 0.01416 min-1 that is 2.29 times greater than pure TiO2. To investigate the role of PVP, we also synthesized Ag@TiO2-5 without PVP. The experimental results show that although Ag@TiO2-5 Cr(VI) reduction performance is superior to pure TiO2, it significantly decreases compared with Ag@TiO2-5P. The results of TEM and optoelectronic testing show that agglomeration of Ag particles leads to a decrease in the photoelectric separation efficiency of Ag@TiO2-5. The smaller Ag particles provide more active sites and demonstrating a stronger overall local surface plasmon resonance (LSPR) effect. DMPO spin-trapping ESR spectra testing indicates that ∙O2- and ∙OH are the main reactive species. This research provides a potential strategy to prepare Ag-based plasma photocatalysts for environment protection.

Role of chromium reductases, antioxidants, and biosorption against oxidative damage of metals by Bacillus cereus.

Current research was performed to look for the performance of Bacillus cereus PY3 for metal detoxification. Strain PY3 was recognized as B. cereus using 16 S rRNA. Higher rate of removal of Zn and Cr (VI) by PY3 was obtained between pH 6-8 and 100-500 µg/mL in 24 h. Highest removal of Cr6+ by strain PY3 was achieved at acidic, neutral, and alkaline atmosphere, 100-300 µg Cr6+ /mL and 25-35°C. Supernatant of PY3 detoxified Cr6+ into Cr3+ then cell pellet (debris) adsorbed them. The mechanism of metal removal was due to the release of cytolic extracts. Release of antioxidants and bio-film played a protective role against cell damage. Metals increased antioxidants and bio-film formation. SEM images showed the smooth external structure of PY3 when cells were exposed to metals thus confirming the role of cells for detoxification. Results Above facts conclude that PY3 can remove metallic pollution in polluted soil.

Novel Ag@NH2-UiO-66(Zr) photocatalyst with controllable charge transfer pathways for efficient Cr(VI) remediation.

Rational fabrication of core-shell photocatalysts to hamper the charge recombination is extraordinarily essential to enhance photocatalytic activity. In this work, core-shell Ag@NH2-UiO-66 (Ag@NU) Schottky heterojunctions with low Ag content (1 wt%) were constructed by a two-step solvothermal method and adopted for Cr(VI) reduction under LED light. Typically, the one with the Ag: NH2-UiO-66 mass ratio (1 : 100) led to 100% Cr(VI) removal within 1 h, superior to bare NH2-UiO-66 and Ag/NH2-UiO-66 (Ag was directly decorated on NH2-UiO-66 surface). The enhanced photocatalytic activity was related to the migration of the electrons on the CB of NH2-UiO-66 to Ag NPs through a Schottky barrier, and thus the undesired charge carriers recombination was avoided. This result was also evidenced by Density functional theory (DFT) calculations. The computational simulations indicate that the introduction of Ag effectively narrowed the band gap of NH2-UiO-66, facilitating the transfer of photo-generated electrons, expanding the light absorption area, and significantly enhancing photocatalytic efficiency. Most importantly, such a core-shell structure can inhibit the formation of •O2-, letting the direct Cr(VI) reduction by photo-excited e-. In addition, this structure can also protect Ag from being oxidized by O2. Ten cyclic tests evidenced the Ag@NU had excellent chemical and structural stability. This research offers a novel strategy for regulating the Cr(VI) reduction by establishing core-shell photocatalytic materials.

Microbially induced carbonate precipitation with Arthrobacter creatinolyticus: An eco-friendly strategy for mitigation of chromium contamination.

Chromium contamination from abandoned industrial sites and inadequately managed waste disposal areas poses substantial environmental threat. Microbially induced carbonate precipitation (MICP) has shown promising, eco-friendly solution to remediate Cr(VI) and divalent heavy metals. In this study, MICP was carried out for chromium immobilization by an ureolytic bacterium Arthrobacter creatinolyticus which is capable of reducing Cr(VI) to less toxic Cr(III) via extracellular polymeric substances (EPS) production. The efficacy of EPS driven reduction was confirmed by cellular fraction analysis. MICP carried out in aqueous solution with 100 ppm of Cr(VI) co-precipitated 82.21% of chromium with CaCO3 and the co-precipitation is positively correlated with reduction of Cr(VI). The organism was utilized to remediate chromium spiked sand and found that MICP treatment decreased the exchangeable fraction of chromium to 0.54 ±â€¯0.11% and increased the carbonate bound fraction to 26.1 ±â€¯1.15% compared to control. XRD and SEM analysis revealed that Cr(III) produced during reduction, influenced the polymorph selection of vaterite during precipitation. Evaluation of MICP to remediate Cr polluted soil sample collected from Ranipet, Tamil Nadu also showed effective immobilization of chromium. Thus, A. creatinolyticus proves to be viable option for encapsulating chromium contaminated soil via MICP process, and effectively mitigating the infiltration of Cr(VI) into groundwater and adjacent water bodies.

Sulfur vacancy-enhanced In2S3-x hollow microtubes for photocatalytic Cr (VI) and tetracycline removal.

Morphological regulation and defect engineering are efficient methods for photocatalytic technology by improving photon absorption and electron dissociation. Herein, In2S3-x hollow microtubes with S-vacancies (MIS) were fabricated via a simple solvothermal reaction using In-based metal-organic frameworks (In-MOFs) as a precursor. Experimental results demonstrate that the hollow structure and optimal S-vacancies can jointly accelerate the photocatalytic reaction, attributed to a larger specific surface area, more active sites, and faster electron transfer efficiency. The champion MIS(2) displayed significantly better photocatalytic activity for Cr(VI) reduction and tetracycline (TC) degradation. The Cr(VI) reduction rate by MIS(2) is 3.67 and 2.82 times higher than those of optimal In2S3 template-free (HIS(2)) and MIS(1) with poor S-vacancies, respectively. The removal efficiency of TC by MIS(2) is 1.37 and 1.15 times higher than those of HIS(2) and MIS(1). Further integration of MIS(2) with aerogel simplifies the recovery process significantly.

Sustainable Cr(VI) reduction in a membrane-less TPBC-MFC driven by solid watermelon rind.

Sustainable Cr(VI) reduction by microbial fuel cell (MFC) is a major challenge due to the electrode passivation and available electron donors. In this study, the chromate removal across a period of more than three months in a membrane-less TPBC-MFC with solid watermelon rind (SWMR) as electron donors was investigated. The TPBC benefited the Cr(VI) reduction and voltage output owing to the enhanced mass transfer. The average Cr(VI) removal efficiency (RE) of 97%, effluent COD of 80 mg/L and voltage output of 130 mV were achieved during the long-term operation on the TPBC-MFC. The SEM-EDS analysis showed that all biofilms were predominated by rod- and coccus-shaped bacteria and the Cr(VI) reduction was mainly carried out by the S-cathode. The XPS, XRD and FT-IR analysis revealed that the major product of cathodic Cr(VI) reduction was a Cr(III) precipitate in the form of Cr(OH)3. Microbial community structure disclosed that fermentation microorganisms (e.g. Anaeroarcus) and electroactive bacteria (e.g. Porphyromonadaceae) jointly responsible for SWMR degradation and electricity generation were dominant at the anode, while the chromate-associated microorganisms (e.g. Comamonadaceae and Cloacibacterium) dominated at the cathode. The biofilms adsorbing Cr(OH)3 precipitates fell off from the cathode periodically to avoid the passivation. Overall, our study suggests a really sustainable approach with which a goal of simultaneously reusing watermelon rind, reducing Cr(VI) and producing electricity was attained perfectly.

A comprehensive study on the co-removal of Cr (VI) and ciprofloxacin via microbial-photocatalytic coupling: Mechanistic insights and performance evaluation.

The increasing contamination of water systems by antibiotics and heavy metals has become a growing concern. The intimately coupled photocatalysis and biodegradation (ICPB) approach offers a promising strategy for the effective removal of mixed pollutants. Despite some prior research on ICPB applications, the mechanism by which ICPB eliminates mixed pollutants remains unclear. In our current study, the ICPB approach achieved approximately 1.53 times the degradation rate of ciprofloxacin (CIP) and roughly 1.82 times the reduction rate of Cr (VI) compared to photocatalysis. Remarkably, after 30 days, the ICPB achieved a 96.1% CIP removal rate, and a 97.8% reduction in Cr (VI). Our investigation utilized three-dimensional fluorescence analysis and photo-electrochemical characterization to unveil the synergistic effects of photocatalysis and biodegradation in removal of CIP and Cr (VI). Incorporation of B-Bi3O4Cl (B-BOC) photocatalyst facilitated electron-hole separation, leading to production of ·O2-, ·OH, and h+ species which interacted with CIP, while electrons reduced Cr (VI). Subsequently, the photocatalytic products were biodegraded by a protective biofilm. Furthermore, we observed that CIP, acting as an electron donor, promoted the reduction of Cr (VI). The microbial communities revealed that the number of bacteria favoring pollutant removal increased during ICPB operation, leading to a significant enhancement in performance.

Effect of oxide interactions on chromium speciation transformation during simulated municipal solid waste incineration.

Chromium released during municipal solid waste incineration (MSWI) is toxic and carcinogenic. The removal of chromium from simulated MSWI flue gas by four sorbents (CaO, bamboo charcoal (BC), powdered activated carbon (PAC), and Al2O3) and the effects of four oxides (SiO2, Al2O3, Fe2O3, and CaO) on chromium speciation transformation were investigated. The results showed that the removal rates of total Cr by the four sorbents were Al2O3 < CaO < PAC < BC, while the removal rates of Cr(VI) by the four sorbents were Al2O3 < PAC < BC < CaO. CaO had a strong oxidizing effect on Cr(III), while BC and PAC had a better-reducing effect on Cr(VI). SiO2 was better for the reduction of Na2CrO4 and K2CrO4 above 1000°C due to its strong acidity, and the addition of CaO significantly inhibited the reduction of Cr(VI). MgCrO4 decomposed above 700°C to form MgCr2O4, and the reaction between MgCrO4 and oxides also existed in the form of a more stable trivalent spinel. Furthermore, when investigating the effect of oxides on the oxidation of Cr(III) in CrCl3, it was discovered that CaO promoted the conversion of Cr(III) to Cr(VI), while the presence of chlorine caused chromium to exist in the form of Cr(V), and increasing the content of CaO and extending the heating time facilitated the oxidation of Cr(III). In addition, silicate, aluminate, and ferrite were generated after the addition of SiO2, Al2O3, and Fe2O3, which reduced the alkalinity of CaO and had an important role in inhibiting the oxidation of Cr(III). The acidic oxides can not only promote the reduction of Cr(VI) but also have an inhibitory effect on the oxidation of Cr(III) ascribed to alkali metals/alkaline earth metals, and the proportion of acidic oxides can be increased moderately to reduce the generation of harmful substances in the hazardous solid waste heat treatment.

Recycling of waste aluminum scraps to fabricate sulfidated zero-valent iron-aluminum particles for enhanced chromate removal.

Massive waste aluminum scraps produced from the spent aluminum products have high electron capacity and can be recycled as an attractive alternative to materials based on zero-valent iron (Fe0) for the removal of oxidative contaminants from wastewater. This study thus proposed an approach to fabricate micron-sized sulfidated zero-valent iron-aluminum particles (S-Al0@Fe0) with high reactivity, electron selectivity and capacity using recycled waste aluminum scraps. S-Al0@Fe0 with a three-layer structure contained zero-valent aluminum (Al0) core, Fe0 middle layer and iron sulfide (FeS) shell. The rates of chromate (Cr(VI)) removal by S-Al0@Fe0 at pH 5.0‒9.0 were 1.6‒5.9 times greater than that by sulfidated zero-valent iron (S-Fe0). The Cr(VI) removal capacity of S-Al0@Fe0 was 8.2-, 11.3- and 46.9-fold greater than those of S-Fe0, zero-valent iron-aluminum (Al0-Fe0) and Fe0, respectively. The chemical cost of S-Al0@Fe0 for the equivalent Cr(VI) removal was 78.5% lower than that of S-Fe0. Negligible release of soluble aluminum during the Cr(VI) removal was observed. The significant enhancement in the reactivity and capacity of S-Al0@Fe0 was partially ascribed to the higher reactivity and electron density of the Al0 core than Fe0. More importantly, S-Al0@Fe0 served as an electric cell to harness the persistent and selective electron transfer from the Al0-Fe0 core to Cr(VI) at the surface via coupling Fe0-Fe2+-Fe3+ redox cycles, resulting in a higher electron utilization efficiency. Therefore, S-Al0@Fe0 fabricated using recycled waste aluminum scraps can be a cost-effective and environmentally-friendly alternative to S-Fe0 for the enhanced removal of oxidative contaminants in industrial wastewater.

Cr(VI) Reduction and Fe(II) Regeneration by Penicillium oxalicum SL2-Enhanced Nanoscale Zero-Valent Iron.

Nanoscale zero-valent iron (nZVI) faces significant challenges in Cr(VI) remediation through aggregation and passivation. This study identified a Cr(VI)-resistant filamentous fungus (Penicillium oxalicum SL2) for nZVI activation and elucidated the synergistic mechanism in chromium remediation. P. oxalicum SL2 and nZVI synergistically and effectively removed Cr(VI), mainly by extracellular nonenzymatic reduction (89.1%). P. oxalicum SL2 exhibited marked iron precipitate solubilization and Fe(II) regeneration capabilities. The existence of the Fe(II)-Cr(V)-oxalate complex (HCrFeC4O9) indicated that in addition to directly reducing Cr(VI), iron ions generated by nZVI stimulated Cr(VI) reduction by organic acids secreted by P. oxalicum SL2. RNA sequencing and bioinformatics analysis revealed that P. oxalicum SL2 inhibited phosphate transport channels to suppress Cr(VI) transport, facilitated iron and siderophore transport to store Fe, activated the glyoxylate cycle to survive harsh environments, and enhanced organic acid and riboflavin secretion to reduce Cr(VI). Cr(VI) exposure also stimulated the antioxidative system, promoting catalase activity and maintaining the intracellular thiol/disulfide balance. Cr(VI)/Fe(III) reductases played crucial roles in the intracellular reduction of chromium and iron, while nZVI decreased cellular oxidative stress and alleviated Cr(VI) toxicity to P. oxalicum SL2. Overall, the P. oxalicum SL2-nZVI synergistic system is a promising approach for regenerating Fe(II) while reducing Cr(VI).

Simultaneous removal of ternary heavy metal ions by a newly isolated Microbacterium paraoxydans strain VSVM IIT(BHU) from coal washery effluent.

In the present work, the removal of Cr (VI), Cd (II) and Pb (II) at 50 mg/L of each metal ion concentration was investigated by Microbacterium paraoxydans strain VSVM IIT(BHU). The heavy metal binding on the bacterial cell surface was confirmed through X-ray photoelectron spectroscopy and energy dispersive X-ray. X-ray photoelectron spectroscopy analysis also confirmed the reduction of Cr (VI) to Cr (III). Heavy metal removal dynamics was investigated by evaluating dimensionless, and the value of Nk (9.49 × 10-3, 9.92 × 10-3 and 1.23 × 10-2 for Cr (VI), Cd (II) and Pb (II) ions) indicated that the removal of heavy metals by bacterial isolate was mixed diffusion and transfer controlled. It was found that both the experimental and predicted values for isolated bacterial strain coincided with each other with a good R2 value in the L-M Algorithm range of 0.94-0.98 for the ternary metal ion system. The bacterial isolate presented a maximum heavy metal ion removal efficiency of 91.62% Cr (VI), 89.29% Pb (II), and 83.29% Cd (II) at 50 mg/L.

Enhanced photocatalytic activity of tin oxide-doped molybdenum disulfide nanohybrids under visible light irradiation: Antibiotics elimination, heavy metal reduction and antibacterial behavior.

Nano-heterojunction photocatalytic can operate removal of pollutants, which is basic for the sustainable development of a clean environment. Herein, we propose a novel MoS2/SnO2 (MS) S-scheme heterojunction by a facile hydrothermal process, which is cheap, easily available, highly visible-light response, and good stability. The MS nano-heterojunction suggested superior performance with the photocatalytic degradation of 97.6% within 100 min for ciprofloxacin (CIP) removal, which was 5.74 and 4.88 folds higher than that of pristine MoS2 and SnO2, respectively. The fabricated MS photocatalysts displayed outstanding photocatalytic efficiency toward Cr (VI) reduction. The removal capability of Cr (VI) reached up to 92.5% within 60 min. The photodegradation efficiency was 5.2 folds that of pristine MoS2. In addition, the antibacterial performance approximately approached 100% for E. coli within 10 min, which was more apparent than the others. A series of excellent results implied that MS nano-heterojunction had a high ultraviolet and visible light absorbance, larger specific surface area, outstanding electron-hole pairs migration and higher capability of photo-response electrons and holes separation rate. This system offers a novel window into the evolution of nano-heterojunction for wastewater treatment and solar energy harvesting applications.

Developing a population-state decision system for intelligently reprogramming extracellular electron transfer in Shewanella oneidensis.

The unique extracellular electron transfer (EET) ability has positioned electroactive bacteria (EAB) as a major class of cellular chassis for genetic engineering aimed at favorable environmental, energy, and geoscience applications. However, previous efforts to genetically enhance EET ability have often impaired the basal metabolism and cellular growth due to the competition for the limited cellular resource. Here, we design a quorum sensing-based population-state decision (PSD) system for intelligently reprogramming the EET regulation system, which allows the rebalanced allocation of the cellular resource upon the bacterial growth state. We demonstrate that the electron output from Shewanella oneidensis MR-1 could be greatly enhanced by the PSD system via shifting the dominant metabolic flux from initial bacterial growth to subsequent EET enhancement (i.e., after reaching a certain population-state threshold). The strain engineered with this system achieved up to 4.8-fold EET enhancement and exhibited a substantially improved pollutant reduction ability, increasing the reduction efficiencies of methyl orange and hexavalent chromium by 18.8- and 5.5-fold, respectively. Moreover, the PSD system outcompeted the constant expression system in managing EET enhancement, resulting in considerably enhanced electron output and pollutant bioreduction capability. The PSD system provides a powerful tool for intelligently managing extracellular electron transfer and may inspire the development of new-generation smart bioelectrical devices for various applications.

Goethite and riboflavin synergistically enhance Cr(VI) reduction by Shewanella oneidensis MR-1.

Bioreduction of Cr(VI) is cost-effective and environmentally friendly, however, the slow bioreduction rate limits its application. In this study, the potential synergistic enhancement of Cr(VI) bioreduction by shewanella oneidensis MR-1 (S. oneidensis) with goethite and riboflavin (RF) was investigated. The results showed that the S. oneidensis reaction system reduce 29.2% of 20 mg/L Cr(VI) after 42 h reaction, while the S. oneidensis/goethite/RF reaction system increased the Cr(VI) reduction rate to 87.74%. RF as an efficient electron shuttle and Fe(II) from goethite bioreduction were identified as the crucial components in Cr(VI) reduction. XPS analysis showed that the final precipitates of Cr(VI) reduction were Cr(CH3C(O)CHC(O)CH3)3 and Cr2O3 and adhered to the bacterial cell surface. In this process, the microbial surface functional groups such as hydroxyl and carboxyl groups participated in the adsorption and reduction of Cr(VI). Meanwhile, an increase in cytochrome c led to an increase in electron transfer system activity (ETSA), causing a significant enhancement in extracellular electron transfer efficiency. This study provides insight into the mechanism of Cr(VI) reduction in a complex environment where microorganisms, iron minerals and RF coexist, and the synergistic treatment method of Fe(III) minerals and RF has great potential application for Cr(VI) detoxification in aqueous environment.

Resistance mechanisms and remediation potential of hexavalent chromium in Pseudomonas sp. strain AN-B15.

The understanding of bacterial resistance to hexavalent chromium [Cr(VI)] are crucial for the enhancement of Cr(VI)-polluted soil bioremediation. However, the mechanisms related to plant-associated bacteria remain largely unclear. In this study, we investigate the resistance mechanisms and remediation potential of Cr(VI) in a plant-associated strain, AN-B15. The results manifested that AN-B15 efficiently reduced Cr(VI) to soluble organo-Cr(III). Specifically, 84.3 % and 56.5 % of Cr(VI) was removed after 48 h in strain-inoculated solutions supplemented with 10 and 20 mg/L Cr(VI) concentrations, respectively. Transcriptome analyses revealed that multiple metabolic systems are responsible for Cr(VI) resistance at the transcriptional level. In response to Cr(VI) exposure, strain AN-B15 up-regulated the genes involved in central metabolism, providing the reducing power by which enzymes (ChrR and azoR) transformed Cr(VI) to Cr(III) in the cytoplasm. Genes involved in the alleviation of oxidative stress and DNA repair were significantly up-regulated to neutralize Cr(VI)-induced toxicity. Additionally, genes involved in organosulfur metabolism and certain ion transporters were up-regulated to counteract the starvation of sulfur, molybdate, iron, and manganese induced by Cr(VI) stress. Furthermore, a hydroponic culture experiment showed that toxicity and uptake of Cr(VI) by plants under Cr(VI) stress were reduced by strain AN-B15. Specifically, strain AN-B15 inoculation increased the fresh weights of the wheat root and shoot by 55.5 % and 18.8 %, respectively, under Cr(VI) stress (5 mg/L). The elucidation of bacterial resistance to Cr(VI) has an important implication for exploiting microorganism for the effective remediation of Cr(VI)-polluted soils.

Construction of UiO-66-NH2 decorated by MoS2 QDs as photocatalyst for rapid and effective visible-light driven Cr(VI) reduction.

The photoactive metal-organic frameworks (MOFs) are good candidates for photocatalysts, but the quick electron-hole pairs recombination has greatly restricted the photocatalytic ability of MOFs. To improve the photoactivity of MOFs, MOFs-based composite materials have been extensively studied. Here, we successfully integrated MoS2 quantum dots (QDs) with UiO-66-NH2 for the first time under hydrothermal conditions. The as-prepared MoS2 QDs/UiO-66-NH2 (MS-U) had good visible light response ability (absorption edge at 445 nm), and charge separation and transfer ability, which lays the foundation for the photocatalytic Cr(VI) reduction. Photocatalytic studies revealed that MoS2 QDs-5/UiO-66-NH2 (MS-U-5) had superior Cr(VI) reduction activity than pure MoS2 QDs and UiO-66-NH2. MS-U-5 could remove 98% Cr(VI) at pH= 2 with visible light irradiation for 20 min, which is the fastest visible light driven Cr(VI) reduction rate among the reported MOFs-based composite photocatalysts without the presence of any cocatalysts or scavengers as far as we know. Importantly, MS-U-5 could be reused at least three times. In the end, the possible electron transfer path and mechanism of Cr(VI) reduction was also investigated.