ABSTRACT
A bacterial strain CC-CTC003T was isolated from a synthetic wooden board. Cells of strain CC-CTC003T were Gram-stain-negative, rod-shaped, motile by gliding and formed yellow colonies. Optimal growth occurred at 25 °C, pH 7 and in the presence of 1â% NaCl. The phylogenetic analyses based on 16S rRNA genes revealed that strain CC-CTC003T belonged to the genus Flavobacterium and was most closely related to Flavobacterium cerinum (95.3â% sequence identity), Flavobacterium maris (94.9â% sequence identity), Flavobacterium qiangtangense (94.8â%) and Flavobacterium subsaxonicum (94.7â%) and had less than 94.7â% sequence similarity to other members of the genus. Average nucleotide identity (ANI) values between strain CC-CTC003T and the type strains of other closely related species were 70.1-74.1â%. The digital DNA-DNA hybridization (dDDH) with F. cerinum was 19.4â%. Strain CC-CTC003T contained C15â:â0, iso-C15â:â0, iso-C15â:â0 3-OH, iso-C17â:â0 3-OH, summed feature 3 (C16â:â1 ω6c / C16â:â1 ω7c) and summed feature 9 (C16â:â0 10-methyl / iso-C17â:â1 ω9c) as the predominant fatty acids. The polar lipid profile consisted of phosphatidylethanolamine, four uncharacterized aminophospholipids, two aminolipids and one unidentified glycolipid. The major polyamine was sym-homospermidine and contained MK-6 as major isoprenoid quinone. The DNA G+C content of the genomic DNA was 39.2 mol%. On the basis of the phylogenetic inference and phenotypic data, strain CC-CTC003T should be classified as a novel species, for which the name Flavobacterium supellecticarium sp. nov. is proposed. The type strain is CC-CTC003T (=BCRC 81146T=JCM 32838T).