Carbon usage in yellow-fleshed Manihot esculenta storage roots shifts from starch biosynthesis to cell wall and raffinose biosynthesis via the myo-inositol pathway.

Cassava is a crucial staple crop for smallholder farmers in tropical Asia and Sub-Saharan Africa. Although high yield remains the top priority for farmers, the significance of nutritional values has increased in cassava breeding programs. A notable negative correlation between provitamin A and starch accumulation poses a significant challenge for breeding efforts. The negative correlation between starch and carotenoid levels in conventional and genetically modified cassava plants implies the absence of a direct genomic connection between the two traits. The competition among various carbon pathways seems to account for this relationship. In this study, we conducted a thorough analysis of 49 African cassava genotypes with varying levels of starch and provitamin A. Our goal was to identify factors contributing to differential starch accumulation. Considering carotenoid levels as a confounding factor in starch production, we found that yellow- and white-fleshed storage roots did not differ significantly in most measured components of starch or de novo fatty acid biosynthesis. However, genes and metabolites associated with myo-inositol synthesis and cell wall polymer production were substantially enriched in high provitamin A genotypes. These results indicate that yellow-fleshed cultivars, in comparison to their white-fleshed counterparts, direct more carbon toward the synthesis of raffinose and cell wall components. This finding is underlined by a significant rise in cell wall components measured within the 20 most contrasting genotypes for carotenoid levels. Our findings enhance the comprehension of the biosynthesis of starch and carotenoids in the storage roots of cassava.

Influence of exogenous 24-epibrassinolide on improving carotenoid content, antioxidant capacity and gene expression in germinated maize seeds.

BACKGROUND: Carotenoids have various physiological functions, such as immune regulation and cancer prevention. Germination could further improve the content of carotenoids in maize seeds. In this study, yellow maize seeds (Suyu 29) were soaked and germinated with different concentrations of 24-epibrassinolide. The changes of germination percentage, sprout length, bioactive components, antioxidant capacity and carotenoid content of the maize seeds were analyzed. Additionally, the relative expression of key genes in the carotenoid synthesis pathway was investigated. RESULTS: The results showed that the sprout length, germination percentage, soluble protein, free amino acids, proline, endogenous abscisic acid, vitamin C, total phenolics and carotenoids displayed a significant increasing trend compared with the control group (P < 0.05). The activity of superoxide dismutase and peroxidase increased by 55.1% and 58.5% versus the control group, and the antioxidant capacity of 2,2-diphenyl-1-picrylhydrazyl, 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) and ferric reducing antioxidant power was 19.8%, 13.4% and 44.1% higher than that of the control group (P < 0.05). Compared with the control group, the expression of genes was significantly up-regulated (P < 0.05). Under the treatment of 0.1 mg L-1 of 24-epibrassinolide, carotenoid content reached the highest value. The carotenoids showed a positive correspondence with antioxidant enzyme activity, antioxidant capacity and total phenolics content (P < 0.05). CONCLUSION: This study showed that 0.1 mg L-1 of exogenous 24-epibrassinolide promoted the accumulation of carotenoids and improved the antioxidant capacity and the quality of germinated maize seeds. It could provide a method for the development of germinated maize products enriched in carotenoids. © 2024 Society of Chemical Industry.

Identification of TaGL1-B1 gene controlling grain length through regulation of jasmonic acid in common wheat.

Grain length is one of the most important factors in determining wheat yield. Here, a stable QTL for grain length was mapped on chromosome 1B in a F10 recombinant inbred lines (RIL) population, and the gene TaGL1-B1 encoding carotenoid isomerase was identified in a secondary large population through multiple strategies. The genome-wide association study (GWAS) in 243 wheat accessions revealed that the marker for TaGL1-B1 was the most significant among all chromosomes. EMS mutants of TaGL1 possessed significantly reduced grain length, whereas TaGL1-B1-overexpressed lines possessed significantly increased grain length. Moreover, TaGL1-B1 strongly interacted with TaPAP6. TaPAP6-overexpressed lines had significantly increased grain length. Transcriptome analysis suggested that TaPAP6 was possibly involved in the accumulation of JA (jasmonic acid). Consistently, JA content was significantly increased in the TaGL1-B1 and TaPAP6 overexpression lines. Additionally, the role of TaGL1-B1 in regulating carotenoids was verified through QTL mapping, GWAS, EMS mutants and overexpression lines. Notably, overexpression of TaGL1-B1 significantly increased wheat yield in multiple locations. Taken together, overexpression of TaGL1-B1 enhanced grain length, probably through interaction with TaPAP6 to cause the accumulation of JA that improved carotenoid content and photosynthesis, thereby resulted in increased wheat yield. This study provided valuable genes controlling grain length to improve yield and a potential insight into the molecular mechanism of modulating JA-mediated grain size in wheat.

Extraction of Bioactive Compounds from C. vulgaris Biomass Using Deep Eutectic Solvents.

C. vulgaris microalgae biomass was employed for the extraction of valuable bioactive compounds with deep eutectic-based solvents (DESs). Particularly, the Choline Chloride (ChCl) based DESs, ChCl:1,2 butanediol (1:4), ChCl:ethylene glycol (1:2), and ChCl:glycerol (1:2) mixed with water at 70/30 w/w ratio were used for that purpose. The extracts' total carotenoid (TCC) and phenolic contents (TPC), as well as their antioxidant activity (IC50), were determined within the process of identification of the most efficient solvent. This screening procedure revealed ChCl:1,2 butanediol (1:4)/H2O 70/30 w/w as the most compelling solvent; thus, it was employed thereafter for the extraction process optimization. Three extraction parameters, i.e., solvent-to-biomass ratio, temperature, and time were studied regarding their impact on the extract's TCC, TPC, and IC50. For the experimental design and process optimization, the statistical tool Response Surface Methodology was used. The resulting models' predictive capacity was confirmed experimentally by carrying out two additional extractions under conditions different from the experimental design.

The new kid on the block: a dominant-negative mutation of phototropin1 enhances carotenoid content in tomato fruits.

Phototropins, the UVA-blue light photoreceptors, endow plants to detect the direction of light and optimize photosynthesis by regulating positioning of chloroplasts and stomatal gas exchange. Little is known about their functions in other developmental responses. A tomato Non-phototropic seedling1 (Nps1) mutant, bearing an Arg495His substitution in the vicinity of LOV2 domain in phototropin1, dominant-negatively blocks phototropin1 responses. The fruits of Nps1 mutant were enriched in carotenoids, particularly lycopene, compared with its parent, Ailsa Craig. On the contrary, CRISPR/CAS9-edited loss of function phototropin1 mutants displayed subdued carotenoids compared with the parent. The enrichment of carotenoids in Nps1 fruits is genetically linked with the mutation and exerted in a dominant-negative fashion. Nps1 also altered volatile profiles with high levels of lycopene-derived 6-methyl 5-hepten2-one. The transcript levels of several MEP and carotenogenesis pathway genes were upregulated in Nps1. Nps1 fruits showed altered hormonal profiles with subdued ethylene emission and reduced respiration. Proteome profiles showed a causal link between higher carotenogenesis and increased levels of protein protection machinery, which may stabilize proteins contributing to MEP and carotenogenesis pathways. The enhancement of carotenoid content by Nps1 in a dominant-negative fashion offers a potential tool for high lycopene-bearing hybrid tomatoes.

Content of Carotenoids, Violaxanthin and Neoxanthin in Leaves of Triticum aestivum Exposed to Persistent Environmental Pollutants.

Persistent pollutants such as pharmaceuticals, pesticides, musk fragrances, and dyes are frequently detected in different environmental compartments and negatively impact the environment and humans. Understanding the impacts of diffuse environmental pollutants on plants is still limited, especially at realistic environmental concentrations of contaminants. We studied the effects of key representatives of two major classes of environmental pollutants (nine different antibiotics and six different textile dyes) on the leaf carotenoid (violaxanthin and neoxanthin) content in wheat (Triticum aestivum L.) using different pollutant concentrations and application times. The wheat plants were watered with solutions of selected environmental pollutants in two different concentrations of 0.5 mg L-1 and 1.5 mg L-1 for one week (0.5 L) and two weeks (1 L). Both categories of pollutants selected for this study negatively influenced the content of violaxanthin and neoxanthin, whereas the textile dyes represented more severe stress to the wheat plants. The results demonstrate that chronic exposure to common diffusively spread environmental contaminants constitutes significant stress to the plants.

Effects of Long-Term Exposure to Low-Power 915 MHz Unmodulated Radiation on Phaseolus vulgaris L.

The morphophysiological response of Phaseolus vulgaris L. to low-power electromagnetic radiation was investigated in order to assess the potential harmful effects of long-term continuous exposure. The plants were grown in two separate electromagnetic field (EMF) shielded rooms, in a controlled, greenhouse-like environment. One batch was continuously irradiated during the growth period (from sowing to maturity) and the other one was used as a reference. An unmodulated signal at 915 MHz (the central frequency between the uplink and downlink of the GSM900 mobile communications band) was used, with a maximum power density of 10 mW/m2 measured near the plants. The plants were analyzed using ultraviolet-visible, statistical, morphometric, and electron microscopy methods. Significant differences were observed regarding the height of the plants, number of inflorescences, and chlorophyll and carotenoid content, all closely connected with the ultrastructural changes observed in the leaves. The irradiated batch grew higher (19% increase in plant height, 20% increase in stem and leaves' dry mass), with 18% fewer inflorescences, and extremely long roots (34% increase in dry mass). The ultrastructure of the irradiated leaves showed irregular cells and a higher content of plastoglobules in the chloroplasts. All results indicate that the irradiated plants suffered significant morphological modifications during their long-term exposure to the specific EM radiation. Bioelectromagnetics. © 2020 Bioelectromagnetics Society.

Standard Analytical Methods, Sensory Evaluation, NIRS and Electronic Tongue for Sensing Taste Attributes of Different Melon Varieties.

Grafting by vegetables is a practice with many benefits, but also with some unknown influences on the chemical composition of the fruits. Our goal was to assess the effects of grafting and storage on the extracted juice of four orange-fleshed Cantaloupe type (Celestial, Donatello, Centro, Jannet) melons and two green-fleshed Galia types (Aikido, London), using sensory profile analysis and analytical instruments: An electronic tongue (E-tongue) and near-infrared spectroscopy (NIRS). Both instruments are known for rapid qualitative and quantitative food analysis. Linear discriminant analysis (LDA) was used to classify melons according to their varieties and storage conditions. Partial least square regression (PLSR) was used to predict sensory and standard analytical parameters. Celestial variety had the highest intensity for sensory attributes in Cantaloupe variety. Both green and orange-fleshed melons were discriminated and predicted in LDA with high accuracies (100%) using the E-tongue and NIRS. Galia and Cantaloupe inter-varietal classification with the E-tongue was 89.9% and 82.33%, respectively. NIRS inter-varietal classification was 100% with Celestial variety being the most discriminated as with the sensory results. Both instruments, classified different storage conditions of melons (grafted and self-rooted) with high accuracies. PLSR showed high accuracy for some standard analytical parameters, where significant differences were found comparing different varieties in ANOVA.

Stability and genotype by environment interaction of provitamin A carotenoid and dry matter content in cassava in Uganda.

Efforts are underway to develop staple crops with improved levels of provitamin A carotenoids to help combat dietary vitamin A deficiency (VAD), which has afflicted the health of resource-poor people in the developing world. As a staple crop for more than 500 million people in sub-Saharan Africa, cassava enriched with provitamin A carotenoids could have a widespread nutritional impact. To this effect, 13 provitamin A clones were evaluated in a randomized complete block design in six environments to assess genotype by environment interaction (GEI) effects for total carotenoid (TCC) and dry matter content (DMC) in roots. Additive main effect and multiplicative interaction analysis showed significant variation among genotypes for TCC, DMC, fresh root weight and harvest index. Environmental effects were non-significant for TCC, but GEI effects were significantly large for all traits measured. There were significant temporal increments for all traits measured within 12 months after planting. TCC correlated negatively with DMC, illustrating an important challenge to overcome when developing provitamin A cassava varieties without compromising DMC, which is a major farmer-preference trait. Nonetheless, best performing genotypes were identified for TCC, DMC and FRW, and these could constitute genetic resources for advancement or developing breeding populations through hybridization.

Cistus creticus subsp. eriocephalus as a Model for Studying Plant Physiological and Metabolic Responses to Environmental Stress Factors.

Variations in physiology and metabolic products of Cistus creticus subsp. eriocephalus along an altitudinal gradient (350-750 m.a.s.l.) within the Monti Lucretili Regional Natural Park (central Italy) were studied. The results showed that the phenol production was in relationship with the net photosynthetic rates and the chlorophyll content. In particular, the increasing caffeic acid (CA) content with altitude suggested its role in providing an additional photo-protection mechanism, by its ability to consume photochemical reducing power and acting as an alternative C-atom sink under high light conditions. The metabolic production was tested by high performance thin layer chromatography (HPTLC) fingerprint analysis, highlighting the potential of this technique in biologic studies.

Rapid Screening of Microalgae as Potential Sources of Natural Antioxidants.

In order to rapidly screen microalgae species as feedstocks for antioxidants, extracts were obtained from 16 microalgae strains (under 11 genera, 7 classes) using two methods: a one-step extraction with ethanol/water and a three-step fractionating procedure using hexane, ethylacetate, and water successively. Measuring the total phenol content (TPC), total carotenoid content (TCC), and antioxidant activity of the extracts, indicating TPC and TCC, played an important role in determining the antioxidant activity of the microalgae. A weighted scoring system was used to evaluate the antioxidant activity, and the scores of microalgal samples from two extraction methods were calculated using the same system. Among the investigated microalgae, Euglena gracilis SCSIO-46781 had the highest antioxidant score, contributing to high TPC and TCC, followed by Arthrospira platensis SCSIO-44012, Nannochloropsis sp. SCSIO-45224, Phaeodactylum tricornutum SCSIO-45120, and Nannochloropsis sp. SCSIO-45006, respectively. Additionally, the above-mentioned five strains are currently being applied in commercial production, indicating this system could be effective not only for screening microalgal antioxidants, but also for screening microalgal species/strains with strong adaptation to environmental stress, which is a critical trait for their commercial cultivation.

Influence of Air-Drying Conditions on Quality, Bioactive Composition and Sensorial Attributes of Sweet Potato Chips.

The drying process is an essential thermal process for preserving vegetables and can be used in developing dried products as healthy alternative snacks. The effects of air-drying conditions using a convection dryer with hot air at different temperatures (60°, 65°, 70°, 75°, and 80 °C, in the range 5-200 min, at a fixed air speed of 2.3 m/s) were tested on the quality of slices (2.0 ± 0.1 mm) of dried sweet potato (Bellevue PBR). For each time and temperature, drying condition, physicochemical parameters (moisture content, CIELab color, texture parameters, total phenolic and carotenoid contents) and a sensory evaluation by a panel at the last drying period (200 min) were assessed. Drying time was shown to have a more significant effect than temperature on the quality of dried sweet potato as a snack, except for carotenoid content. Given the raw tuber content, thermal degradation (p < 0.05) of total phenolic compounds (about 70%), regardless of tested conditions, contrasted with the higher stability of total carotenoids (<30%). The dried product, under optimal conditions (≥75 °C for 200 min), achieved a moisture content (≤10%) suitable for preservation, providing a crispy texture with favourable sensory acceptance and providing a carotenoid content similar to the raw product.

Process optimization and evaluation of the effects of different time-temperature sous vide cooking on physicochemical, textural, and sensory characteristics of whiteleg shrimp (Litopenaeusvannamei).

The objective of the current study was to optimize the cook-chill conditions of high-value whiteleg shrimp (Litopenaeus vannamei) processed using the sous vide (SV) technique and to assess the effects of various time-temperature combinations on the physicochemical, textural, and sensory qualities. For optimization, a Response Surface Methodology (RSM) approach utilizing a Central Composite Design (CCD) was adopted. Optimum SV cooking conditions to acquire minimum texture (hardness) of 7235 g was 13.48 min and 81.87 °C, expressible moisture of 18.48% was 14.5 min and 84.5 °C, and cook loss of 5.58% was 5 min and 75 °C. Texture (hardness) and expressible moisture decreased while cooking loss increased with increasing time-temperature treatment. Redness and yellowness values increased (p < 0.05) with increasing SV cooking time-temperature, but lightness values were nearly consistent in all treatments. With increasing time and temperature, TBARs and total carotenoid content increased (p < 0.05). However, the TBARs values were within accepted limits and ranged from 0.05 to 0.08 mg malonaldehyde/kg. Sensory evaluation indicated that all SV cooked samples were well accepted, with overall scores ≥7. These results suggest that the SV cooking temperature and time had a substantial impact on the textural, physicochemical, and sensory characteristics of shrimp. In addition, increasing time-temperature increased cooking and moisture loss, but decreased hardness and higher sensory scores made the product more acceptable to consumers.

Leaf photosynthetic pigment as a predictor of leaf maximum carboxylation rate in a farmland ecosystem.

The leaf maximum rate of carboxylation (Vcmax) is a key parameter of plant photosynthetic capacity. The accurate estimation of Vcmax is crucial for correctly predicting the carbon flux in the terrestrial carbon cycle. Vcmax is correlated with plant traits including leaf nitrogen (Narea) and leaf photosynthetic pigments. Proxies for leaf chlorophyll (Chlarea) and carotenoid contents (Cararea) need to be explored in different ecosystems. In this study, we evaluated the relationship between leaf maximum rate of carboxylation (scaled to 25°C; Vcmax25) and both leaf Narea and photosynthetic pigments (Chlarea and Cararea) in winter wheat in a farmland ecosystem. Our results showed that Vcmax25 followed the same trends as leaf Chlarea. However, leaf Narea showed smaller dynamic changes before the flowering stage, and there were smaller seasonal variations in leaf Cararea. The correlation between leaf Vcmax25 and leaf Chlarea was the strongest, followed by leaf Cararea and leaf Narea (R2 = 0.69, R2 = 0.47 and R2 = 0.36, respectively). The random forest regression analysis also showed that leaf Chlarea and leaf Cararea were more important than leaf Narea for Vcmax25. The correlation between leaf Vcmax25 and Narea can be weaker since nitrogen allocation is dynamic. The estimation accuracy of the Vcmax25 model based on Narea, Chlarea, and Cararea (R2 = 0.75) was only 0.05 higher than that of the Vcmax25 model based on Chlarea and Cararea (R2 = 0.70). However, the estimation accuracy of the Vcmax25 model based on Chlarea and Cararea (R2 = 0.70) was 0.34 higher than that of the Vcmax25 model based on Narea (R2 = 0.36). These results highlight that leaf photosynthetic pigments can be a predictor for estimating Vcmax25, expanding a new way to estimate spatially continuous Vcmax25 on a regional scale, and to improve model simulation accuracy.

Enhancement of body coloration of sword-tail fish (Xiphophorus helleri): Plant-derived bio-resources could be converted into a potential dietary carotenoid supplement.

The effects of natural plant-derived carotenoid sources on body coloration and growth performance of Sword-Tail Fish (Xiphophorus helleri) were investigated using four different dietary treatments; Commercial Feed (CF), China Rose Flower (CRF), Marigold Flower (MFF), and Carrot (CAF). CF was used as the control treatment without a natural source of carotenoids. The sources were mixed with feed (15 g/100 g) during the diet preparation. Fish were fed with four treatments twice a day for eight weeks, and then sampling was done weekly. A total of 96 fish were used for the experiment, and the mean initial weights were 3.314 g. MFF-treated fishes showed better growth performance in terms of mean weight, length and Specific Growth Rate (SGR), and carotenoid gain than the other treatments. The lowest Food Conversion Ratio (FCR) was also observed in the MFF-treated fish. The study suggested that marigold flower mixed feed could increase the body coloration and boost the growth performance of sword-tail fish and may be manufactured.

Effects of ultrasonic treatment on color, carotenoid content, enzyme activity, rheological properties, and microstructure of pumpkin juice during storage.

Freshly squeezed pumpkin juice (Cucurbita moschata D.) was sonicated at various power levels at a constant frequency of 25 kHz and a treatment time of 10 min. Samples were stored in the dark for 0, 4, 8, and 12 days at 4 °C and were subsequently analyzed. The combined effects of power level and storage period on color parameters, carotenoid content, particle size distribution, cloud value, rheological characteristics, and microstructure were investigated. The results showed ultrasonic-treated samples had little effect on carotenoid content, cloud value, particle size distribution, and polydispersity during storage compared to those of the untreated samples. The L⁎, a⁎, b⁎, and C* values decreased significantly during 8-12 days of storage, resulting in a significant increase in ΔE, especially 400 W/10 min-treated samples. Meanwhile, the enzyme activity and rheological properties increased significantly on storage days 8-12. However, the microstructure of all samples did not change significantly during storage. Based on these results, during the storage period, the physical and chemical properties of 400 W/10 min-ultrasonic treated pumpkin juice were retained more than those in the untreated pumpkin juice. Therefore, ultrasonic treatment has broad application prospects in preserving bioactive substances and physicochemical properties and improving the storage life of fresh pumpkin juice.

Correlation of Skin Carotenoid Content with 3-Day Dietary Intake in Community Dwelling Older Adults.

Pressure-mediated reflection spectroscopy (RS) measures skin carotenoid content (SCC). Continued validation of this method is pertinent to validate its use as an objective measure of fruit and vegetable intake. This study aimed to assess relationships between SCC scores and self-reported dietary carotenoid intake from 3-day food records in community dwelling older adults. This was a cross-sectional analysis of baseline data among cognitively normal older adults (n=95) participating in the Nutrition Interventions for Cognitive Enhancement (NICE) study. Food-derived vitamin A (R=0.24, p<0.05) and food plus supplemental vitamin A (R=0.29, p<0.01) were correlated with SCC. All food-derived carotenoids (R: 0.20-0.39, p<0.05) except beta-cryptoxanthin and lycopene were correlated with SCC. Lutein + zeaxanthin from food more strongly correlated with SCC (R=0.38, p<0.001) than combined food plus supplemental intake (R=0.31, p=0.002). Correlations for total fruit and SCC (R=0.23, p=0.02), total vegetable and SCC (R= 0.31, p=0.002), and combined total fruit and vegetable and SCC (R=0.35, p<0.001) were significant; no sub-categories of fruit or vegetables except dark green vegetables (Rho=0.4, p<0.001) had significant correlations with SCC. The current study demonstrates RS-derived SCC scores correlate with 3-day self-reported intakes of F/V and certain carotenoids in community dwelling older adults.

Enrichment of flaxseed (Linum usitatissimum) oil with carotenoids of sea buckthorn pomace via ultrasound-assisted extraction technique: Enrichment of flaxseed oil with sea buckthorn.

Currently, flaxseed oil is used as an important functional food constituent owing to its large content of omega-3 fatty acids. However, flaxseed oil does not contain carotenoids that could enhance the oxidative stability of the oil. In this study, carotenoids extracted from sea buckthorn pomace were used to enrich cold-pressed flaxseed oil via an ultrasound-assisted extraction technique (UAE). The process parameters were optimized through Box-Behnken design to maximize the carotenoid content in the flaxseed oil. The results obtained by statistical analysis indicated that the yield of 14.02 mg/L of carotenoid content was found in the enriched flaxseed oil at 75.6 min, feed to oil ratio of 19.9 (wt. basis), and amplitude 80.81%. Further, UAE at optimum process parameters was compared with the conventional extraction (CE) method, and it was found that UAE had ~ 49 wt% of higher carotenoid content relative to CE. The physicochemical properties of the enriched flaxseed oil were determined to evaluate the effects of carotenoid enrichment in the flaxseed oil. Based on the outcomes of the present investigation, enriched flaxseed oil could be the potential source for the pharmaceuticals and nutraceuticals industry.

Mitigating the Negative Effect of Drought Stress in Oat (Avena sativa L.) with Silicon and Sulphur Foliar Fertilization.

A field experiment was carried out in the 2020-2021 growing season, aiming at investigating the abiotic stress tolerance of oat (Avena sativa L.) with silicon and sulphur foliar fertilization treatments and monitoring the effect of treatments on the physiology, production and stress tolerance of winter oat varieties. In the Hungarian national list of varieties, six winter oat varieties were registered in 2020, and all of the registered varieties were sown in a small plot field experiment in Debrecen, Hungary. The drought tolerance of the oat could be tested, because June was very dry in 2021; the rainfall that month totaled 6 mm only despite a 30-year average of 66.5 mm, and the average temperature for the month was 3.2 °C higher than the 30-year average. Foliar application of silicon and sulphur fertilizers caused differences in the photosynthesis rate, total conductance to CO2, transpiration, water use efficiency, leaf area, chlorophyll content, carotenoid content, thousand kernel weight (TKW) and yield of winter oat. The application of silicon significantly increased the photosynthesis rate (16.8-149.3%), transpiration (5.4-5.6%), air-leaf temperature difference (16.2-43.2%), chlorophyll (1.0%) and carotenoid (2.5%) content. The yield increased by 10.2% (Si) and 8.0% (Si plus S), and the TKW by 3.3% (Si) and 5.0% (Si plus S), compared to the control plots. The plants in the control plots assimilated less CO2 while transpiring 1 m3 water more than in the Si, S or Si plus S fertilized plots. The effect of the silicon varied from 9.0 to 195.4% in water use efficiency (WUE) in the three development stages (BBCH52, BBCH65 and BBCH77). A lower leaf area index was measured in the foliar fertilized plots; even so, the yield was higher, compared to that from the control plots. Great variation was found in response to the foliar Si and S fertilization among winter oat varieties-in WUE, 2.0-43.1%; in total conductance to CO2, 4.9-37.3%; in leaf area, 1.6-34.1%. Despite the droughty weather of June, the winter oat varieties produced a high yield. The highest yield was in 'GK Arany' (7015.7 kg ha-1), which was 23.8% more than the lowest yield ('Mv Kincsem', 5665.6 kg ha -1). In the average of the treatments, the TKW increased from 23.9 to 33.9 g (41.8%). 'Mv Hópehely' had the highest TKW. Our results provide information about the abiotic stress tolerance of winter oat, which, besides being a good model plant because of its drought resistance, is an important human food and animal feed.

Synergism of Dietary Co-Supplementation with Lutein and Bile Salts Improved the Growth Performance, Carotenoid Content, Antioxidant Capacity, Lipid Metabolism, and Lipase Activity of the Marbled Spinefoot Rabbitfish, Siganus rivulatus.

A 60-day feeding trial was conducted to assess the effects of dietary supplementation with bile salts (BS), lutein (LTN), and their combination on growth, survival, carotenoid content, and antioxidant status of rabbitfish (Siganus rivulatus) larvae. Fish were fed four isonitrogenous (34.39% protein) and isoenergetic (20.57 kJ/g) diets supplemented with BS (0.15 g kg-1), LTN (0.1 g kg-1), BS+LTN (0.15 and 0.1 g kg-1, respectively), and a non-supplemented control diet. The results revealed that fish fed BS+LTN had the highest significant specific growth rate (4.37% day-1), feed efficiency (46.55%), and survival (97.78%). Lutein supplementation improved whole-body protein content, meanwhile, fish fed a BS-supplemented diet had a higher lipid content. The carotenoid deposition was significantly increased with LTN and BS+LTN in skin, muscle, and whole body compared to the control and BS treatment. All dietary supplementation of BS and LTN showed significant improvement in total antioxidant capacity, catalase, and glutathione peroxidase activities. Additionally, LTN alone or BS+LTN significantly reduced malondialdehyde levels by 5.30 and 29.91%, respectively compared to the control. BS supplementation modulated aminopeptidases activities, triglycerides, cholesterol, and increased the activity of pancreatic lipase. Therefore, it could be inferred that dietary supplementation with LTN in combination with BS could improve the growth performance, carotenoid deposition, antioxidant status, lipid digestion, and metabolism of S. rivulatus.