The oral 13C-bicarbonate technique for determination of energy expenditure in dogs: dietary and environmental factors affecting the respiratory quotient and 13C recovery factor.

The oral 13C-bicarbonate technique (o13CBT) can be used for short-term measurements of CO2 production (RCO2) and energy expenditure (EEx). The method relies on appropriate estimates for the respiratory quotient (RQ) and recovery factor (RF) of 13C. Four Retriever dogs were included in four experiments to validate the o13CBT against indirect calorimetry (IC), and determine RQ and RF; Expt. 1: feeding different protein:fat:carbohydrate ratios [in % of metabolisable energy]: 25:33:42 in a maintenance (Mnt.) diet; 38:26:36 in a high-protein high-fibre (HFibre) diet and 27:56:17 in a high-fat (HFat) diet, simultaneously with start of measurements (T0); Expt. 2: the Mnt. diet at T0 or 4 h postprandial (T4); Expt. 3: T4 at different ambient temperatures, 22°C and 15°C; Expt. 4: T4 after 1 h physical activity. The RCO2 and EEx were determined from the respiration chamber measurements made simultaneously with IC and the o13CBT (o13CBTonline), and in Expts. 1 and 2, also on two consecutive days using o13CBT with collection of breath into breath bags (o13CBTbreathbags). The RQ values obtained at T0 reflected dietary compositions, with the highest least square mean (LSM) of 0.954  for the Mnt. diet, 0.905 for the HFibre and 0.877 for the HFat diet (p < 0.05). An increased interval between meal and measurement period decreased RQ significantly (p < 0.05) in Expt. 2, LSM being 0.954 at T0 and 0.909 at T4. Ambient temperature (Expt. 3) and physical activity (Expt. 4) did not influence postprandial RQ. The RF values were not significantly affected by diet (Expt. 1). Measurements starting at T0 (Expt. 2) resulted in higher (p < 0.05) RF values than at T4 (LSM = 0.971 and 0.836, respectively). The ambient temperatures (Expt. 3) did not influence postprandial RF. However, when dogs were physically active prior to measurements (Expt. 4), RF values (LSM = 1.019) were higher (p < 0.05) than when resting only (LSM = 0.836). Calculations based on RQ and RF determined in each experiment resulted in RCO2 and EEx values which were not different regardless of method used, except for Expt. 1 where EEx-values [kJ · kg BW-0.75 · d-1] were higher (p < 0.05) when measured with o13CBTbreathbags (460) than by IC (421) and o13CBTonline (420). Provided accurate RQ and RF values, the o13CBTbreathbags can be used as an independent and minimally invasive research tool to determine EEx in dogs under carefully standardised conditions.

Use of indirect calorimetry to evaluate utilization of energy in lactating Jersey dairy cattle consuming diets with increasing inclusion of hydrolyzed feather meal.

A study using indirect calorimetry and 12 lactating multiparous Jersey cows (53 ± 23 d in milk at the beginning of the experiment; mean ± standard deviation) was conducted to evaluate the utilization of energy in cattle consuming diets containing increasing hydrolyzed feather meal (HFM). A triplicated 4 × 4 Latin square design with 35-d periods (28-d adaption and 4-d collections) was used to compare 4 different dietary treatments. Treatments contained (DM basis) HFM at 0% (0HFM), 3.3% (3.3HFM), 6.7% (6.7MFM), and 10.0% (10HFM). Diets were formulated such that HFM replaced blood meal and nonenzymatically browned soybean meal. With increasing HFM, linear increases were observed for dietary NEL content (1.61, 1.64, 1.69, and 1.70 ± 0.042 Mcal/kg of DM for 0HFM, 3.3HFM, 6.7MFM, and 10HFM, respectively), and the efficiency of converting ME to NEL (0.708, 0.711, 0.717, and 0.719). Apparent total-tract digestibility of CP linearly decreased with increasing HFM (63.4, 61.1, 59.9, and 58.6 ± 1.46% for 0HFM, 3.3HFM, 6.7MFM, and 10HFM, respectively), whereas long-chain fatty acid digestibility increased with increasing HFM (77.2, 77.7, 78.5, and 80.6 ± 1.30%). With increased inclusion of HFM, fecal N excretion increased (199, 230, 239, 237 ± 12.1 g/d for 0HFM, 3.3HFM, 6.7MFM, and 10HFM, respectively), whereas urinary N excretion decreased (166, 151, 155, and 119 ± 14.8 g/d). Increasing the concentration of HFM resulted in a quadratic effect on DMI (19.6, 20.2, 20.3, and 19.1 ± 0.79 kg/d for 0HFM, 3.3HFM, 6.7MFM, and 10HFM, respectively) and milk yield (31.7, 32.0, 31.9, and 29.7 ± 1.32 kg/d). Increasing HFM linearly decreased the milk protein concentration (3.34, 3.29, 3.23, and 3.23 ± 0.158 for 0HFM, 3.3HFM, 6.7MFM, and 10HFM, respectively) and yield (1.05, 1.05, 1.02, and 0.96 ± 0.040 kg). The inclusion of HFM did not affect energy-correct milk yield (average of 39.3 ± 1.54). Results of this study suggest that HFM can increase dietary NEL content compared with blood meal and nonenzymatically browned soybean meal and maintained energy-corrected milk yield; however, feeding HFM at greater than 6.7% of diet DM decreased DMI, and protein availability may have been reduced with increased HFM, leading to a linear decrease in milk protein concentration and yield.

Estimation of metabolisable energy and net energy of rice straw and wheat straw for beef cattle by indirect calorimetry.

Two experiments were conducted to estimate the metabolisable energy (ME) and net energy (NE) of rice straw and wheat straw for beef cattle. In each experiment, 16 Wandong bulls (Chinese indigenous yellow cattle) were assigned to 4 dietary treatments in a completely randomised design. Four dietary treatments included one corn silage-concentrate basal diet and three test diets in which the basal diet was partly substituted by rice straw (Exp. 1) or wheat straw (Exp. 2) at 100, 300 and 600 g/kg. Total collection of faeces and urine was conducted for 5 consecutive days after a 2-week adaption period, followed by a 4-d period where gas exchange measurements were measured by an open-circuit respiratory cage. Linear regression equations of rice straw- or wheat straw-associated ME and NE contribution in test diets against rice straw or wheat straw substitution amount were developed to predict the ME and NE values of rice straw and wheat straw. These regression equations resulted in ME and NE values (dry matter basis) of 6.76 and 3.42 MJ/kg for rice straw and 6.43 and 3.28 MJ/kg for wheat straw, respectively. The NE and ME requirement for maintenance of Wandong cattle fed a straw-based diet were 357 and 562 kJ·kg-0.75·d-1, respectively. The regression-derived ME and NE have lower standard errors and coefficients of variation than those estimated by any single substitution ratio. Our study found that the regression method based on multiple point substitution is more reliable than the substitution method for energy evaluation of feedstuffs for beef cattle.

Effect of sorghum distillers dried grains with solubles and microbial enzymes on metabolizable and net energy values of broiler diets.

There is generally no information regarding the influence of distillers dried grains with solubles (DDGS) on the net energy (NE) value of broiler diets. It was hypothesized that the ME content of DDGS may overestimate its productive energy, leading to higher heat increment when used in broiler diets. Furthermore, it was of interest to investigate the potential benefits of a mixture of carbohydrases and protease on energy partitioning in diets containing DDGS. In this study, 2 evaluations, comparative slaughter (CS) or indirect calorimetry (IC), were conducted using the same batch of birds and feed. Two levels of DDGS (0 or 30%) without or with an enzyme mixture were used in a 2 × 2 factorial arrangement of treatments. The CS evaluation used 240 male Ross 308 broiler chicks with 6 replicates (10 birds each) per treatment from d 18 to 28. Carcass energy values were examined in 2 birds per replicate on each of d 18 and 28 to determine heat production (HP), NE, and ME. The IC evaluation used 32 birds acclimatized to 16 closed-circuit calorimeters (2 birds per chamber) following energy partitioning measurement by gaseous exchange and total excreta collection from 18 to 24 d. Birds fed DDGS had increased feed efficiency during starter and grower phases (P < 0.001). The CS showed no difference in HP between birds fed diets with or without DDGS. When measured by IC, birds fed diets containing DDGS had higher HP and lower NE (P < 0.01). Enzyme supplementation had no effect on HP, ME, or NE:ME ratio measured using CS or IC but increased NE and NE intake (P < 0.01) from d 18 to 28. Carcass fat retention was decreased (P < 0.05) and there was a lower NE (P < 0.01) in birds fed the DDGS-diets. This study showed that 30% dietary DDGS affects energy partitioning in broilers with more heat produced, decreased dietary NE, and less fat retained in the carcass.

Effects of photoperiod on food intake, activity and metabolic rate in adult neutered male cats.

With the continued rise in feline obesity, novel weight management strategies are needed. To date, strategies aimed at altering physical activity, an important factor in weight maintenance, have been lacking. Photoperiod is known to cause physiological changes in seasonal mammals, including changes in body weight (BW) and reproductive status. Thus, our objective was to determine the effect of increased photoperiod (longer days) on voluntary physical activity levels, resting metabolic rate (RMR), food intake required to maintain BW, and fasting serum leptin and ghrelin concentrations in adult cats. Eleven healthy, adult, neutered, male domestic shorthair cats were used in a randomized crossover design study. During two 12-week periods, cats were exposed to either a short-day (SD) photoperiod of 8 h light: 16 h dark or a long-day (LD) photoperiod of 16 h light: 8 h dark. Cats were fed a commercial diet to maintain baseline BW. In addition to daily food intake and twice-weekly BW, RMR (via indirect calorimetry), body composition [via dual-energy X-ray absorptiometry (DEXA)] and physical activity (via Actical activity monitors) were measured at week 0 and 12 of each period. Fasting serum leptin and ghrelin concentrations were measured at week 0, 6 and 12 of each period. Average hourly physical activity was greater (p = 0.008) in LD vs. SD cats (3770 vs. 3129 activity counts/h), which was primarily due to increased (p < 0.001) dark period activity (1188 vs. 710 activity counts/h). This corresponded to higher (p < 0.0001) daily metabolizable energy intake (mean over 12-week period: 196 vs. 187 kcal/day), and increased (p = 0.048) RMR in LD cats (9.02 vs. 8.37 kcal/h). Body composition, serum leptin and serum ghrelin were not altered by photoperiod. More research is needed to determine potential mechanisms by which these physiological changes occurred and how they may apply to weight management strategies.

The oral (13)C-bicarbonate technique for estimation of energy expenditure in dogs: validation against indirect calorimetry.

To get more knowledge about the energy requirements of dogs and to formulate appropriate feeding guidelines, it is essential to determine their energy expenditure (EE) in a reliable and feasible way. In this study, the non-invasive oral stable isotope (13)C-bicarbonate technique (o(13)CBT) was validated against indirect calorimetry (IC) for the determination of CO2-production and EE in dogs. Eleven privately owned dogs were simultaneously measured with IC and the o(13)CBT after being fasted overnight. All dogs were measured twice on two separate days. For calculation, measurements were divided into two groups depending on dogs' behaviour during the measurement. Dogs of Group 1 (n = 17) were resting calmly in the chamber and dogs of Group 2 (n = 5) were more active. Mean heart rate was significantly higher in Group 2 (102 beats per minute [bpm]) than in Group 1 (77 bpm) (p < 0.001). Within groups, the CO2-production and EE [kJ d(-1) kg BW(-0.75)] estimated by the o(13)CBT or IC did not differ significantly (Group 1: [Formula: see text] = 368; EEIC = 363; Group 2: [Formula: see text] = 701; EEIC = 718). However, the estimated (13)C recovery factor (RF) for the estimation of CO2-production was significantly different between Groups 1 and 2 (0.72 and 0.94, respectively, p < 0.001). The respiratory quotient (RQ), which is needed for the estimation of EE, did not differ between groups. This study shows that the non-invasive o(13)CBT can be used for accurate estimation of the CO2-production rate and EE in resting dogs. A value of 0.77 can be applied as an estimate of the RQ in fasted dogs and 0.72 as an appropriate estimate for RF when dogs are resting calmly during the measurements.

Research Note: Effect of fasting time on the fasting heat production, blood metabolites, and body components of layer-type pullets.

Fasting heat production (FHP) is used to assess the maintenance net energy requirement of animals. Herein, the FHP of layer-type pullets was estimated. In trial 1, 16 40-day-old Jingfen layer-type pullets were divided into 4 groups of 4 chickens and placed in 4 respiratory chambers. Pullets had free access to feed and water. After 4-d acclimatization, feed was withdrawn, and chickens were measured for FHP for 3 consecutive days. In trial 2, twenty-four 40-day-old pullets were placed in 4 respiratory calorimetry chambers, with 6 pullets per chamber. After 4-d acclimatization, one chamber was randomly selected and all pullets in the chamber was sampled at 5, 25, 50, or 65 h after feed withdrawal. The result showed that FHP declined with fasting time and reached the lowest level between 48 and 72 h. Respiratory quotient was decreased (P < 0.05) between 24 and 48 h compared with that in the first 24 h after fasting. The FHP in the light period showed a significant to decline with fasting time (P < 0.01), whereas the FHP in the dark period was decreased (P < 0.01) 24 h after fasting. Body weight, thigh mass, and abdominal fat decreased (P < 0.05) at 25 h after fasting. Serum glucose were increased (P < 0.01) and while triglycerides were significantly decreased (P < 0.01) at 50 h compared with that at 5 and 25 h time point. The result suggests that the adequate measuring period for FHP for layer-type pullets is from 24 to 48 h after fasting. The FHP of 7-wk-old layer-type pullets was 562.20 kJ/kg of BW0.75/d under a 10-h light and 14-h dark lighting regime.

Development of a dynamic model for prediction of energy in milk protein, lactose, fat, and enteric methane emissions in goats based on energy balance and indirect calorimetry studies.

Feed costs are overwhelmingly the largest expense for dairy producers. Thus, improving milk production efficiency (milk fat and protein are the main incomes for farmers) is of great economic importance in the dairy industry. The main objective of this study was to develop a dynamic energy partitioning model to describe and quantify how dietary energy from carbohydrate, protein, and fat is transferred to milk (protein, lactose, and fat) in dairy goats. In addition, due to increasing worldwide concerns regarding livestock contribution to global warming, methane (CH4) emission was quantified. For modeling purposes, 158 individual goat observations were used and randomly split into 2/3 for model development and 1/3 for internal evaluation. For external evaluation, 20 different energy balance studies from the literature (77 observations) were evaluated. The Root Mean Square Prediction Error (RMSPE) was 13.2% for loss of energy in CH4, 16.8% for energy in fat, 19.4% for energy in protein, and 22.3 energy in lactose. Mean bias was around zero for all variables and the slope bias was zero for milk energy in lactose, close to 1% for milk fat (1.01%), and around 3% and 10% for protein and CH4, respectively. Random bias was greater than 85% for energy in CH4 and milk energy components indicating non-systematic errors and that the equation in the model fitted the data properly. Analyses of residuals appeared to be randomly distributed around zero. Slopes of regression lines for residuals vs. predicted were positive for milk fat energy, zero for lactose, and negative for milk energy in protein and CH4. This model suggested for use with mixed diets and by-products to obtain balanced macronutrient supply, methane emissions, and milk performance during mid lactation could be an interesting tool to help farmers simulate scenarios that increase milk fat and protein, evaluate CH4 emissions, without the costs of running animal trials.

The present model using mixed diets with different by-products to obtain macronutrient balance, methane emission, and milk performance during mid lactation could be an interesting tool to help farmers, without the costs of running animal experiments. The dietary change from grain-based to partial replacement with agro-industrial-byproducts in mid-lactation dairy goats was accompanied by transformations in carbohydrate and fat energy transfer to support production. The output underscored that both oxidation of carbohydrate and fat interact to maintain milk energy output.

Quantifying resistant starch using novel, in vivo methodology and the energetic utilization of fermented starch in pigs.

To quantify the energy value of fermentable starch, 10 groups of 14 pigs were assigned to one of two dietary treatments comprising diets containing 45% of either pregelatinized (P) or retrograded (R) corn starch. In both diets, a contrast in natural ¹³C enrichment between the starch and nonstarch components of the diet was created to partition between enzymatic digestion and fermentation of the corn starch. Energy and protein retention were measured using indirect calorimetry after adapting the pigs to the diets for 3 wk. Fecal ¹³C enrichment was higher in the R-fed pigs (P < 0.001) and 43% of the R resisted enzymatic digestion. Energy retained as protein was unaffected and energy retained as fat was 29% lower than in P-fed pigs (P < 0.01). Prior to the morning meal, end products of fermentation substantially contributed to substrate oxidation in the R-fed pigs. During the 3-4 h following both meals, heat production was higher (P < 0.05) in P-fed pigs, but this was not preferentially fueled by glucose from corn starch. Digestible energy intake, metabolizable energy intake, and energy retention were reduced (P < 0.05) in R-fed pigs compared with P-fed pigs by 92, 54, and 33 kJ/(kg°·75 · d), respectively. Therefore, the energy values of fermented resistant starch were 53, 73, and 83% of the digestible, metabolizable, and net energy values of enzymatically degradable starch, respectively. Creating a contrast in natural ¹³C enrichment between starch and nonstarch dietary components provides a promising, noninvasive, in vivo method for estimating the proportion of dietary starch fermented in the gastrointestinal tract.

Introduction of adult cats to indirect calorimetry respiration chambers causes increased energy expenditure and respiratory quotient that decrease following acclimation.

OBJECTIVE: To replicate a previously defined behavioral procedure to acclimate adult cats to temporary restriction in indirect calorimetry chambers and measure energy expenditure and respiratory quotient changes during acclimation. ANIMALS: 8 healthy adult cats (4 spayed females, and 4 neutered males; mean ± SEM age, 2.5 ± 1.5 years; mean body weight, 4.8 ± 1.8 kg). PROCEDURES: Cats underwent a 13-week incremental acclimation procedure whereby cats were acclimated to the chambers in their home environment (weeks 1 to 3), to the study room (weeks 4 to 6), and to increasing lengths of restriction within their home environment (weeks 7 to 8) and the chambers (weeks 9 to 13). Cat stress score, respiratory rate, fearfulness (assessed with a novel object test), energy expenditure, and respiratory quotient were measured. Data were analyzed by use of a repeated-measures mixed model. RESULTS: Stress, based on cat stress scores, fearfulness, and respiration, peaked at weeks 4, 9, and 10 but returned to baseline levels by week 11. Energy expenditure and respiratory quotient peaked at weeks 10 and 11, respectively, but were reduced significantly by weeks 11 and 13, respectively. All cats returned to baseline by the end of the study and were deemed fully acclimated. CLINICAL RELEVANCE: Changes in perceived stress level, energy expenditure, and respiratory quotient at various stages of the acclimation procedure suggest that stress should be considered a significant variable in energy balance measurements when indirect calorimetry is used in cats. An incremental acclimation procedure should therefore be used to prepare cats for the temporary space restriction necessary for indirect calorimetry studies.

Influence of a high-protein diet on energy balance in obese cats allowed ad libitum access to food.

The influence of a high-protein [HP, 47% of metabolizable energy (ME)] diet on energy balance was evaluated in obese cats allowed ad libitum access to food. Energy intake, body weight, body composition, energy expenditure, and concentrations of hormones and metabolites associated with carbohydrate and lipid metabolism (glucose, insulin, free fatty acids, triglycerides and leptin) were measured in cats after consuming either a moderate protein (MP, 27% of ME) or HP diet for 4 months. Indirect respiration calorimetry showed that resting and total energy expenditure (kJ/day) adjusted for either body weight or lean body mass was increased in cats consuming the HP in relation to MP diets. However, voluntary energy intake also was increased in the HP treatment and, thus, there was no difference in body weight between animals consuming the two diets. Body composition measurements using deuterium oxide dilution showed that dietary protein content did not alter amounts of either lean body mass or fat mass. No significant differences (p > 0.05) were observed between the two treatment groups for blood glucose, free fatty acid or leptin concentrations, although there was a trend (p = 0.054) towards an increase of serum insulin concentrations in the cats eating the HP diet. This study showed that short-term ad libitum feeding of an HP diet did not reduce food intake or promote weight loss in obese cats. However, energy expenditure was increased in the HP diet group and it is possible that this effect of HP might help promote weight loss when energy intake is restricted.

Development of a dynamic energy-partitioning model for enteric methane emissions and milk production in goats using energy balance data from indirect calorimetry studies.

The main objective of this study was to develop a dynamic energy balance model for dairy goats to describe and quantify energy partitioning between energy used for work (milk) and that lost to the environment. Increasing worldwide concerns regarding livestock contribution to global warming underscore the importance of improving energy efficiency utilization in dairy goats by reducing energy losses in feces, urine and methane (CH4). A dynamic model of CH4 emissions from experimental energy balance data in goats is proposed and parameterized (n = 48 individual animal observations). The model includes DM intake, NDF and lipid content of the diet as explanatory variables for CH4 emissions. An additional data set (n = 122 individual animals) from eight energy balance experiments was used to evaluate the model. The model adequately (root MS prediction error, RMSPE) represented energy in milk (E-milk; RMSPE = 5.6%), heat production (HP; RMSPE = 4.3%) and CH4 emissions (E-CH4; RMSPE = 11.9%). Residual analysis indicated that most of the prediction errors were due to unexplained variations with small mean and slope bias. Some mean bias was detected for HP (1.12%) and E-CH4 (1.27%) but was around zero for E-milk (0.14%). The slope bias was zero for HP (0.01%) and close to zero for E-milk (0.10%) and E-CH4 (0.22%). Random bias was >98% for E-CH4, HP and E-milk, indicating non-systematic errors and that mechanisms in the model are properly represented. As predicted energy increased, the model tended to underpredict E-CH4 and E-milk. The model is a first step toward a mechanistic description of nutrient use by goats and is useful as a research tool for investigating energy partitioning during lactation. The model described in this study could be used as a tool for making enteric CH4 emission inventories for goats.

Minimum dietary methionine requirements in Miniature Dachshund, Beagle, and Labrador Retriever adult dogs using the indicator amino acid oxidation technique.

The objective of this study was to determine the minimum requirement (MR) for methionine (Met), when cyst(e)ine (Cys) is provided in excess, in adult dogs of three different breed sizes using the indicator amino acid (AA) oxidation (IAAO) technique. In total, 12 adult dogs were used: 1 neutered and 3 spayed Miniature Dachshunds (4.8 ± 0.4 kg body weight [BW], mean ± SD), 4 spayed Beagles (9.5 ± 0.7 kg BW, mean ± SD), and 4 neutered Labrador Retrievers (31.8 ± 1.7 kg BW, mean ± SD). A deficient Met basal diet with excess Cys was formulated. Dogs were fed the basal diet randomly supplemented with different Met-Alanine (Ala) solutions to achieve final Met concentrations in experimental diets of 0.21%, 0.26%, 0.31%, 0.36%, 0.41%, 0.46%, and 0.66% (as-fed basis). After 2 d of adaptation to the experimental diets, dogs underwent individual IAAO studies. During the IAAO study day, the total feed was divided into 13 equal meals; at the sixth meal, dogs were fed a bolus of l-[1-13C]-phenylalanine (Phe), and thereafter, l-[1-13C]-Phe was supplied with every meal. The total production of 13CO2 during isotopic steady state was determined by the enrichment of 13CO2 in breath samples, and the total production of CO2 measured using indirect calorimetry. The mean MR for Met and the upper 95% confidence limit (CL) were determined using a two-phase linear mixed-effects regression model. For Miniature Dachshunds, the MR for Met was between the first two dietary Met concentrations and is, therefore, between 35.7 and 44.1 mg.kg BW-1·d-1 (0.21% to 0.26%, as-fed basis; no requirement could be determined on a metabolic BW basis). For Beagles and Labrador Retrievers, the MR for Met was 57.5 and 50.4 mg.kg BW-1·d-1, 107.7 and 121.8 mg/kg BW^0.75, or 0.338 and 0.360%, respectively (as-fed basis). The upper 95% CL of Met requirements was 77.9 and 72.4 mg.kg BW-1·d-1, 147.8 and 159.6 mg/kg BW^0.75,or 0.458 and 0.517% for Beagles, and Labradors, respectively (as-fed basis). When pooling data from Beagles and Labrador Retrievers, the MR and upper 95% CL were 56.0 and 75.8 mg.kg BW-1·d-1 or 118.4 and 150.5 mg/kg BW^0.75 or 0.360% and 0.482% (as-fed basis). In conclusion, the MR and the upper 95% CL for Met are different for Dachshunds when compared with Beagles and Labrador Retrievers. Using this low-protein diet, the estimated upper 95% CL Met requirement for Beagles and Labrador is higher than those recommended in the National Research Council (NRC), but NRC is similar to the estimated upper 95% CL for Dachshunds.

Adult dogs of different breed sizes have similar threonine requirements as determined by the indicator amino acid oxidation technique.

Threonine (Thr) requirements for immature (growing) Beagles have been determined, but little knowledge is available on Thr requirements for maintenance in mature dogs. Moreover, differences of Thr requirements among different breeds or sizes of adult dogs have not been investigated. The objective of the present study was to determine Thr requirements in adult dogs of three different breeds using the indicator amino acid oxidation (IAAO) technique. In total, 13 adult dogs were used, 4 Miniature Dachshunds (5.8 ± 0.4 kg body weight [BW]; 3 spayed and 1 neutered), 4 spayed Beagles (9.3 ± 0.6 kg BW), and 5 neutered Labrador Retrievers (30.5 ± 1.7 kg BW). Dogs were fed a Thr-deficient diet (Thr = 0.23%) and randomly allocated to receiving one of seven concentrations of Thr supplementation (final Thr concentration in experimental diets was 0.23%, 0.33%, 0.43%, 0.53%, 0.63%, 0.73%, and 0.83%; as fed basis) for 2 d. After 2 d of adaptation to the experimental diets, dogs underwent individual IAAO studies. During the IAAO studies, total daily feed was divided into 13 equal meals; at the sixth meal, dogs were fed a bolus of l-[1-13C]-Phenylalanine (Phe) (9.40 mg/kg BW), and thereafter, l-[1-13C]-Phe (2.4 mg/kg BW) was supplied with every meal. Before feeding the next experimental diet, dogs were fed a Thr-adequate basal diet for 4 d (Thr = 0.80% as fed basis) in known amounts that maintained individual dog BW. Total production of 13CO2 during isotopic steady state was determined by enrichment of 13CO2 in breath samples and total production of CO2 measured using indirect calorimetry. The mean requirements for Thr, defined as the breakpoint, and the 95% confidence interval (CI) were determined using a two-phase linear regression model. For Miniature Dachshunds, the two-phase model was not significant, and Thr requirements could not be determined. Mean Thr requirements for Beagles and Labradors were 72.2 and 64.1 mg/kg BW on an as-fed basis, respectively. The requirement for Thr between these two dog breeds was not different (P > 0.10). Thus, the data for Beagles and Labradors were pooled and a mean requirement for Thr was determined at 66.9 mg/kg BW, and the 95% CI was estimated at 84.3 mg/kg BW. In conclusion, estimated Thr requirements for Beagles and Labradors did not differ, and these recommendations are higher than those suggested by NRC (2006) and AAFCO (2014) for adult dogs at maintenance.

Effects of diet type on nutrient utilization and energy balance in drylot heifers1.

Feeding cattle in intensified settings allows cow-calf producers to decrease their reliance on grazed forage and utilize alternative feedstuffs. During times of intense management, diet type may alter energy utilization. Fourteen pregnant MARC III heifers (405 ± 44 kg BW) were used in a 180 d experiment to determine effects of diet type on nutrient and energy utilization. Heifers were randomly assigned to one of two treatments, a forage diet (FOR; 2.10 Mcal metabolizable energy [ME]/kg; 95.75% forage) or a concentrate diet (CONC; 2.94 Mcal ME/kg; 71% concentrate), and individually fed to meet maintenance energy requirements (0.135 Mcal ME/kg BW0.75). The CONC diet contained dry-rolled corn, corn stalks (10.16 cm grind size), soybean meal, corn silage (approximately 45% corn grain; stored in a plastic bag), dicalcium phosphate, urea, and a premix pellet; FOR contained alfalfa hay (harvested at mid-bloom), corn silage, dicalcium phosphate, and a premix pellet. Measurements of energy intake and digestibility were measured over a 4-d period on days 116, 172, and 235 of gestation. Using portable headbox calorimeters, measurements of O2, CO2, and CH4 gases were collected over a period of 24 h. Data were analyzed in a completely randomized design with diet as fixed effect. Dry matter and organic matter digestibility were greater for CONC than FOR (P < 0.01). Intake of gross energy (GE) and digestible energy (DE) were greater for FOR (P < 0.01), but by design, ME intake was not different between treatments (P = 0.26). Energy lost as methane (% of GE intake) was not different between treatments (P = 0.49). The ratio of ME to DE was greater for CONC (86.8 vs. 82.8; P = 0.01) than FOR. Heat production relative to ME was not different between treatments (P = 0.85). Maternal tissue energy did not differ and was 1.2 Mcal/d for CONC and 0.9 Mcal/d for FOR (P = 0.73). Greater nitrogen (N) consumption was observed for FOR (192.2 g/d) than CONC (134.0 g/d; P < 0.01), and retained N was greater for FOR than CONC (P < 0.01) on days 116 and 235 of gestation. Neither concentrate-based or forage-based diets affected body condition score (P = 0.26). Heifers fed concentrate-based diets retained more energy in part because they had larger calves, but this energy was not recovered in maternal tissue.

Technical note: a new facility for continuous respiration measurements in lactating cows.

An open-circuit indirect calorimetry system consisting of 4 climate-controlled respiration chambers for cattle has been constructed and validated. The system allows for the continuous monitoring of O(2), CO(2), and CH(4) concentrations in chamber air, and the simultaneous determination of feed and water intake, overall physical activity, position changes, standing and lying times, and animal behavior. For complete balance trials, feces, urine, and milk can be collected quantitatively. Most importantly, lactating cows can be milked in the chamber, and blood samples can be drawn from permanent catheters without disruption of the measurements. The investigator, on entering the chamber, wears a facemask connected to the ambient air during the whole milking process. Data are routed to a data acquisition system with appropriate data evaluation software developed in our research unit. Thus, dynamic changes of the above-named parameters during the course of the day or of longer time periods can be monitored. Such data are critical for understanding the complex regulation and interplay of feed intake, energy metabolism, climatic conditions, and milk production.

Technical note: development of an indirect calorimetry system to determine heat production in individual lactating sows1.

The ability to determine total heat production (THP) in individual sows and litters can be logistically difficult and often requires the use of multiple animals to generate data on a per room basis. Furthermore, these systems may be costly to construct, precluding their use by many researchers. Therefore, the objective was to develop a low-cost indirect calorimetry system to determine THP in individual lactating sows and litters. Six indirect calorimeters were constructed to house 1 sow and litter in a crate throughout farrowing and a 21-d lactation period. Farrowing crates were placed within a high-density polyethylene pan filled with water and then a polyvinyl chloride frame was constructed around the crate. The frame provided a structure to hold the inlet and outlet air pipes, feed and water inlets, air circulation fans, and a polyethylene plastic sheet that was secured at the bottom of the frame and submerged under water to maintain an air tight seal. Chamber accuracies for O2 and CO2 were evaluated by ethanol combustion. One week pre-farrowing, 6 pregnant multiparous sows (parity 2.9 ± 0.9; 218.3 ± 38.6 kg BW) were housed individually in each farrowing crate and the calorimeters were maintained at thermoneutral conditions (20.9 ± 2.6°C and 43.7 ± 18.6% relative humidity) throughout lactation. On lactation day 4, 8, 14, and 18, indirect calorimetry was performed on all sows and their litters, as well as 2 piglets from a sentinel litter to determine THP and the respiratory quotient (RQ). Sentinel piglet data were used to estimate THP and RQ for the sows independent of the litter. Sow + litter THP (kcal/h) increased (P = 0.01; 16.6%) on day 8 compared to day 4 and was greater (27.3%) on day 14 and day 18 compared to day 4 and day 8. Sow THP was greater (P = 0.01) on day 8 (401.19 ± 17.15 kcal/h) and day 14 (430.79 ± 12.42 kcal/h) compared to day 4 (346.16 ± 16.62 kcal/h), and was greater on day 14 compared to day 8, and on day 18 (386.16 ± 20.02 kcal/h) compared to day 14. No sow + litter RQ differences (P = 0.21; 1.02 ± 0.04) were detected by day of lactation. However, sow RQ was reduced (P = 0.01) on day 14 (0.98 ± 0.02) compared to day 4 (1.03 ± 0.03), day 8 (1.02 ± 0.02), and day 18 (1.04 ± 0.03). In summary, this cost-effective system (total cost: $1,892 USD) can allow researchers to accurately evaluate THP in individual lactating sows and their litters.

Estimation of the net energy and protein requirements for maintenance of male arctic foxes (Alopex lagopus) during the growth period1,2.

The maintenance requirements of net energy and net protein were assumed to represent the most accurate and important values totally for the animal's utilization. The objective of this experiment was to determine the net energy and net protein requirements for maintenance of growing arctic foxes. The experiments was evaluated using regression models estimated from data collected by means of indirect calorimetry, nitrogen balance trials, and digestion and metabolism experiments. Thirty-six growing arctic foxes (3 487 ± 261.7 g) at the age of 85 days were randomly assigned to four groups with 9 animals in each group. Arctic foxes were fed a complete formula diet at four intake levels (100%, or 80%, 60%, and 40% of feed requirements) from 24 July 2017 to 23 September 2017. Arctic foxes in each treatment were kept individually in respiration chambers after 1-d adaptation at day 2 for a 3-d balance trial and then at day 5 followed by a 3-d fasting period. The metabolizable energy intake (MEI), heat production in the fed state (HP), and retained energy (RE) of arctic foxes significantly decreased (P < 0.01) as the feed intake level decreased. Fasting heat production (FHP) of arctic foxes was not influenced by feed intake level (P > 0.05). The metabolizable energy maintenance requirement (MEm) and net energy maintenance requirement (NEm) estimated from the linear relationship between RE and MEI were 230 and 217 kJ/kg of body weight BW0.75/d, respectively. The MEm and NEm estimated by logarithmic regression of HP on MEI were 225 and 209 kJ/kg BW0.75/d, respectively. The net N maintenance requirement (NNm) and net protein maintenance requirement (NPm) estimated from the linear relationship between retained nitrogen (RN) and daily nitrogen intake (NI) were 179.6 mg/kg BW0.75/d and 1.123 g/kg BW0.75/d, respectively. It is concluded that NEm and NPm values obtained fill the net energy and protein requirements shortage, and provide the basic data for establishing the standard of nutrition demand of breeding arctic foxes in China.

Tryptophan requirements in small, medium, and large breed adult dogs using the indicator amino acid oxidation technique1.

Tryptophan (Trp) is an indispensable amino acid (AA) for dogs of all life stages; however, although Trp requirements for growing dogs are derived from 3 dose-response studies, there are no empirical data on Trp requirements for adult dogs at maintenance. The study objective was to determine Trp requirements of adult dogs of 3 different breeds using the indicator amino acid oxidation (IAAO) technique. Four spayed or neutered Miniature Dachshunds (5.28 ± 0.29 kg BW), 4 spayed Beagles (9.32 ± 0.41 kg BW), and 5 neutered Labrador Retrievers (30.51 ± 2.09 kg BW) were used. After a 14-d adaptation to a Trp-adequate basal diet (Trp = 0.482% dry matter), all dogs were fed a mildly Trp-deficient diet for 2 d (Trp = 0.092% dry matter) before being randomly allocated to receiving 1 of 7 concentrations of Trp supplementation (final Trp content in experimental diets was 0.092, 0.126, 0.148, 0.182, 0.216, 0.249, and 0.283% dry matter) and all dogs received all Trp treatments. After 2-d adaptation to the experimental diets, dogs underwent individual IAAO studies. Total feed was divided in 13 equal meals; at the sixth meal, dogs were fed a bolus of L-[1-13C]-Phenylalanine (Phe) (9.40 mg/kg BW), and thereafter, L-[1-13C]-Phe was supplied (2.4 mg/kg BW) with every meal. Total production of 13CO2 during isotopic steady state was determined by enrichment of 13CO2 in breath samples and total production of CO2 measured using indirect calorimetry. The maintenance requirement for Trp and the 95% confidence interval (CI) were determined using a 2-phase linear regression model. Mean Trp requirements were estimated at 0.154, 0.218, and 0.157% (dry-matter) for Dachshunds, Beagles, and Labradors, respectively. The upper 95% CI were 0.187, 0.269, and 0.204% (dry-matter) for Dachshunds, Beagles, and Labradors. In conclusion, estimated Trp requirements are higher for Beagles compared with Labradors or Dachshunds, and all estimated requirements are higher than those currently recommended by the NRC and AAFCO.

Dietary phenylalanine requirements are similar in small, medium, and large breed adult dogs using the direct amino acid oxidation technique.

We have previously determined phenylalanine (Phe) requirements in mature dogs; however, little information is available on differences of Phe minimum requirements on different breed sizes. The objective of this study was to determine Phe requirements in adult dogs of three different breed sizes using the direct AA oxidation (DAAO) technique. In total, 12 adult dogs were used, four Miniature Dachshunds (5.3 ± 0.6 Kg BW; 1.8 ± 0.1 years old; mean ± SD), four Beagles (8.3 ± 0.7 Kg BW; 6.7 ± 0.2 years old; mean ± SD), and four Labrador Retrievers (34.9 ± 2.2 Kg BW; 4.4 ± 1.4 years old; mean ± SD). A basal Phe-deficient diet with excess of tyrosine (Tyr) was formulated. Dogs were randomly fed the basal diet supplemented with increasing levels of Phe; the Phe content in the final experimental diets was 0.24, 0.29, 0.34, 0.44, 0.54, 0.64, and 0.74%. After 2 d of adaptation to the experimental diets, dogs underwent individual DAAO studies. During the DAAO studies, total daily feed was divided in 13 equal meals; at the sixth meal, dogs were fed a bolus of L-[1-13C]-Phe (9.40 mg/kg BW), and thereafter, L-[1-13C]-Phe (2.4 mg/kg BW) was supplied with every meal. Total production of 13CO2 (F13CO2) during isotopic steady state was determined by enrichment of 13CO2 in breath samples and total production of CO2 measured using indirect calorimetry. The mean requirement for Phe and the 95% confidence interval (CI) was determined using a two-phase linear regression model. To account for differences in feed intake, requirements were expressed in mg.kg BW-1.d-1. The mean requirement for Phe were 41.9, 41.3, and 42.6, and upper 95% CI of Phe requirements were 57.3, 58.4, and 64.8 mg.kg BW-1.d-1 for Miniature Dachshunds, Beagles, and Labrador Retrievers, respectively. The mean requirement and the upper 95% CI for the pooled data (all dogs) was 45.3 and 55.4 mg.kg BW-1.d-1, respectively. In conclusion, the Phe requirements for different breeds were similar among dog breeds studied. However, Phe recommendations proposed in this study are lower than those proposed by NRC and AAFCO (mg.kg BW-1.d-1).