ABSTRACT
OBJECTIVES: To present clinical and cytopathological features of nine cases of chordomas, diagnosed over 9 years and confirmed by brachyury (T) immunostaining. METHODS: Conventional cytological smears, stained with Papanicolaou and May-Grünwald Giemsa, along with corresponding histopathological (n = 8) and immunostained sections (n = 8) were reviewed. Immunohistochemical staining was performed on tissue sections by polymer detection technique. RESULTS: Nine tumours occurred in seven males and two females, with age ranging from 36 to 72 years (average = 58.7), in the sacrum (seven) and spine (two). On fine needle aspiration cytology, five cases were either diagnosed with or diagnosed with a suggestion of a chordoma, while three cases were diagnosed with chordoma as a differential diagnosis. On review, smears were moderately cellular, comprising myxoid stroma (9/9), epithelioid cells (9/9), physaliphorous cells (8/9), including binucleation (7/9), prominent nucleolisation (2/9), pleomorphic cells (2/9) and intranuclear inclusions (3/9). Immunohistochemically, tumour cells expressed cytokeratin (4/4), pan cytokeratin (4/4), epithelial membrane antigen (8/8), S100 protein (6/8) and brachyury (8/8). Five patients underwent surgical excision, including two who underwent adjuvant radiotherapy (RT) and four patients who underwent RT. During follow-up (n = 8), a single patient developed recurrence and another presented with metastatic lesions. Finally, five patients were alive with disease (7-53 months); a single patient was free of disease (4 months), and two patients died of disease; the latter cases displayed pleomorphic cells and intranuclear inclusions. CONCLUSIONS: Chordomas can be primarily diagnosed by fine needle aspiration cytology in a typical clinicoradiological setting with a combination of key cytomorphological features. Pleomorphic cells and intranuclear inclusions are associated with a relatively aggressive subtype. An exact diagnosis has treatment implications and requires confirmation by brachyury immunostaining.
Subject(s)
Chordoma/diagnosis , Cytodiagnosis , Fetal Proteins/isolation & purification , T-Box Domain Proteins/isolation & purification , Adult , Aged , Biopsy, Fine-Needle , Chordoma/genetics , Chordoma/pathology , Female , Fetal Proteins/genetics , Gene Expression Regulation, Neoplastic/genetics , Humans , Male , Middle Aged , Mucin-1/genetics , T-Box Domain Proteins/geneticsABSTRACT
Proteomics has brought with it the hope of identifying novel biomarkers for the fetal aneuploidies. This hope is built on the ability of proteomic technologies, such as two-dimensional gel electrophoresis (2-DE) with immobilized pH gradients combined with protein identification by mass spectrometry (MS). The large dynamic range of plasma necessitates the effective removal of abundant plasma proteins to allow analysis of the lower concentration analytes. There are many factors that make this research very challenging, beginning with standardization of sample collection, consistent sample preparation, and continuing through the entire analytical process. Therefore, reproducible sample complexity reduction methods such as depletions or fractionations are an essential first step in biomarker discovery experiments. For qualitative and quantitative evaluation of the proteome, the fluorescent dye stains offer several advantages over traditional staining methods. The sensitivity of the fluorescent dye stains such as SYPRO Ruby is comparable with that of silver staining and also has a broad dynamic range, which allows accurate protein quantification being compatible with MS methods for protein identification. Despite its limitations for proteome analysis 2-DE is currently the workhorse for proteomics. Taking into account the factors such as cost, availability and ease of use, 2-DE electrophoresis is one of the most apposite approaches toward the methodical characterization of proteomes.
Subject(s)
Aneuploidy , Fetal Proteins/metabolism , Prenatal Diagnosis/methods , Proteomics , Biomarkers/blood , Electrophoresis, Gel, Two-Dimensional , Female , Fetal Proteins/genetics , Fetal Proteins/isolation & purification , Fluorescent Dyes , Humans , Maternal-Fetal Exchange , Pregnancy , Reproducibility of Results , Spectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationABSTRACT
We have cloned the gene for a novel Ets-related transcription factor, new Ets-related factor (NERF), from human spleen, fetal liver, and brain. Comparison of the deduced amino acid sequence of NERF with those of other members of the Ets family reveals that the level of homology to ELF-1, which is involved in the regulation of several T- and B-cell-specific genes, is highest. Homologies are clustered in the putative DNA binding domain in the middle of the protein, a basic domain just upstream of this domain, and several shorter stretches of homology towards the amino terminus. The presence of two predominant NERF transcripts in various fetal and adult human tissues is due to at least three alternative splice products, NERF-1a, NERF-1b, and NERF-2, which differ in their amino termini and their expression in different tissues. Only NERF-2 and ELF-1, and not NERF-1a and NERF-1b, function as transcriptional activators of the lyn and blk gene promoters, although all isoforms of NERF bind with affinities similar to those of ELF-1 to a variety of Ets binding sites in, among others, the blk, lck, lyn, mb-1, and immunoglobulin H genes and are expressed at similar levels. Since NERF and ELF-1 are coexpressed in B and T cells, both might be involved in the regulation of the same genes.
Subject(s)
DNA-Binding Proteins/chemistry , Genes , Transcription Factors/chemistry , Transcription Factors/isolation & purification , Adult , Amino Acid Sequence , Animals , Base Sequence , Binding Sites , Cell Line , Cell Line, Transformed , Chlorocebus aethiops , Cloning, Molecular , Fetal Proteins/isolation & purification , Fibroblasts/metabolism , Gene Expression Regulation , HeLa Cells/metabolism , Humans , Mice , Molecular Sequence Data , Multigene Family , Nuclear Proteins , Organ Specificity , RNA Splicing , RNA, Messenger/genetics , RNA, Messenger/isolation & purification , Regulatory Sequences, Nucleic Acid , Sequence Alignment , Sequence Homology, Amino Acid , Transcription Factors/genetics , Transcription Factors/physiology , Transcriptional Activation , src-Family Kinases/geneticsABSTRACT
Formins are key regulators of actin cytoskeletal dynamics that constitute a diverse protein family that is present in all eukaryotes examined. They typically consist of more than 1000 amino acids and are defined by the presence of two conserved regions, namely the formin homology 1 and 2 domains. Additional conserved domains comprise a GTPase-binding domain for activation, a C-terminal autoregulation motif and an N-terminal recognition domain. In this study, the N-terminal region (residues 1-339) of the human formin homology domain-containing protein 1 (FHOD1) was purified and crystallized from 20%(w/v) PEG 4000, 10%(v/v) glycerol, 0.3 M magnesium chloride and 0.1 M Tris-HCl pH 8.0. Native crystals belong to space group P1, with unit-cell parameters a = 35.4, b = 73.9, c = 78.7 A, alpha = 78.2, beta = 86.2, gamma = 89.7 degrees. They contain two monomers of FHOD1 in the asymmetric unit and diffract to a resolution of 2.3 A using a synchrotron-radiation source.
Subject(s)
Fetal Proteins/chemistry , Nuclear Proteins/chemistry , Peptide Fragments/chemistry , Crystallization , Fetal Proteins/isolation & purification , Formins , Humans , Nuclear Proteins/isolation & purification , Peptide Fragments/isolation & purification , Protein Structure, TertiaryABSTRACT
Lymphocyte reactivity to 3-M KCl extracts from fetuses of different species of origin was shown by the macrophage electrophoretic mobility (MEMB) and/or the leukocyte migration inhibition tests in tumor-bearing mice and humans. In chemical carcinogenesis, reactivity was detectable before tumors developed. Six weeks after sc injection of 1,000 micrograms benzo[a]pyrene [(BP) CAS: 50-32-8] into XVII/Bln mice, the MEMB test became positive. The latent period was 15 weeks after 1.0 micrograms BP, indicating a dose-response relationship of the phenomenon. Painting of mouse skin with 7,12-dimethylbenz[a]anthracene [(DMBA) CAS: 57-97-6], croton oil (CAS: 8001-28-3), or benzene (CAS: 71-43-2) had the same sensitizing effect as BP. In contrast to the strong carcinogens BP and DMBA that caused lymphocyte reactivity to persist until tumors developed, benzene and the promoter croton oil induced only a transient effect. Termination of treatment abolished reactivity within 12 weeks. Lymphocyte reactivity to fetal extract in normal cell proliferation was evident from the fact that two-thirds-hepatectomized rats and BCG-treated mice became MEMB-positive. In hepatectomized rats the effect was reversible according to the completion of liver regeneration. Lymphocytes from tumor-bearing mice reacted with mouse and human fetal extract as well as with extracts from different developmental stages of frogs and fish. Fetal extracts were assumed to contain a phylogenetically conserved antigen.
Subject(s)
Antigens, Neoplasm/immunology , Fetal Proteins/immunology , Lymphocytes/immunology , Neoplasms/immunology , Precancerous Conditions/immunology , Animals , Antigens, Neoplasm/isolation & purification , Cell Division , Cell Line , Chick Embryo , Cross Reactions , Embryo, Mammalian/immunology , Embryo, Nonmammalian , Female , Fetal Proteins/isolation & purification , Fetus/immunology , Fishes , Guinea Pigs , Hepatectomy , Humans , Immunity, Cellular , Male , Mice , Neoplasm Transplantation , Neoplasms/chemically induced , Phylogeny , Precancerous Conditions/chemically induced , Pregnancy , Ranidae , Rats , SnakesABSTRACT
Two variants of alpha-fetoprotein in rat amniotic fluid were separated by their different affinity for concanavalin A-Sepharose, which selectively binds alpha-D-manno-pyranosides and alpha-D-glucopyranosides. Both forms had the same mobility upon polyacrylamide gel electrophoresis. The binding of 17beta-estradiol per mg of alpha-fetoprotein, determined both immunologically and electrophoretically, was the same for both variants. These results indicate that a specific carbohydrate portion of the molecule is not necessary for steroid binding.
Subject(s)
Amniotic Fluid/analysis , Estradiol , Fetal Proteins , alpha-Fetoproteins , Animals , Binding Sites , Chromatography, Affinity , Electrophoresis, Polyacrylamide Gel , Female , Fetal Proteins/isolation & purification , Neuraminidase , Pregnancy , Protein Binding , Rats , alpha-Fetoproteins/isolation & purificationABSTRACT
Basic helix-loop-helix-PER-ARNT-SIM (bHLH-PAS) proteins form dimeric transcription factors to mediate diverse biological functions including xenobiotic metabolism, hypoxic response, circadian rhythm and central nervous system midline development. The Ah receptor nuclear translocator protein (ARNT) plays a central role as a common heterodimerization partner. Herein, we describe a novel, embryonically expressed, ARNT interacting protein (AINT) that may be a member of a larger coiled-coil PAS interacting protein family. The AINT C-terminus mediates interaction with the PAS domain of ARNT in yeast and interacts in vitro with ARNT and ARNT2 specifically. AINT localizes to the cytoplasm and overexpression leads to non-nuclear localization of ARNT. A dynamic pattern of AINT mRNA expression during embryogenesis and cerebellum ontogeny supports a role for AINT in development.
Subject(s)
Carrier Proteins/metabolism , Fetal Proteins/metabolism , Receptors, Aryl Hydrocarbon , Transcription Factors/metabolism , Amino Acid Sequence , Animals , Aryl Hydrocarbon Receptor Nuclear Translocator , Binding Sites , Carrier Proteins/genetics , Carrier Proteins/isolation & purification , Cerebellum/embryology , Cloning, Molecular , DNA-Binding Proteins/genetics , DNA-Binding Proteins/metabolism , Embryonic and Fetal Development , Fetal Proteins/genetics , Fetal Proteins/isolation & purification , Gene Expression , Helix-Loop-Helix Motifs , Humans , Hypoxia-Inducible Factor 1 , Hypoxia-Inducible Factor 1, alpha Subunit , Mice , Microtubule-Associated Proteins , Molecular Sequence Data , Nuclear Proteins/genetics , Nuclear Proteins/metabolism , RNA, Messenger , Saccharomyces cerevisiae , Sequence Homology, Amino Acid , Signal Transduction , Subcellular Fractions , Transcription Factors/genetics , Two-Hybrid System TechniquesABSTRACT
Various models of normal and abnormal developmental systems were addressed to get an insight into molecular parameters of cell differentiation at the level of protein gene products. Electrophoretic analysis of heterogeneous protein mixtures permitted qualitative analysis of developing systems, particularly during organogenesis in mammals, as well as of neoplastic growth in the animal and plant kingdoms. From our earlier findings indicating that the definite protein patterns characteristic of adult organs are acquired long after the adult morphological and histological characteristics of these tissues have developed, it has been repeatedly proven that quantitative changes in whole proteins is not a dependable indicator of cell differentiation.
Subject(s)
Fetal Proteins/isolation & purification , Gene Expression Regulation , Neoplasm Proteins/isolation & purification , Animals , Blood Proteins/analysis , Cell Differentiation , Female , Genital Neoplasms, Female/blood , Humans , Isoenzymes , L-Lactate Dehydrogenase/analysis , Male , Mice , Plant Tumors/etiology , Rats , Teratoma/blood , Transcriptional Activation , alpha-Fetoproteins/analysisABSTRACT
We investigated the ontogeny of the human placental membrane somatomedin-C (Sm-C)/insulin-like growth factor I (IGF-I) receptor by affinity labeling with the cross-linking agent disuccinimidyl suberate (DSS). Specific Sm-C receptors, identified from as early as 6 weeks gestation, demonstrated no apparent structural changes through the course of gestation. Second trimester human fetal brain membranes cross-linked to [125I]Sm-C exhibited an identical pattern of receptor binding. These findings in both placenta and fetal brain membrane are consistent with the proposed heterotetrameric structure of the Sm-C/IGF-I receptor. Using a similar DSS cross-linking technique, we identified Sm-C-binding proteins in second trimester amniotic fluid [apparent molecular mass (Mr), 38,000 and 35,000] and term cord plasma (Mr, 41,000, 38,000, and 35,000). Identically sized binding components (Mr, 35,000-45,000) were also found in membrane preparations of preterm placenta and brain after cross-linking. Evidence that these binding species represent contamination of preterm membrane preparations with soluble amniotic fluid and/or fetal plasma Sm-C-binding proteins and that they are not derived from membrane receptors is as follows: (1) these binding species, like amniotic fluid and cord plasma binding proteins, were insensitive to competition with insulin (in concentrations as high as 0.6 mg/ml), a characteristic not shared with membrane receptor binding components; (2) these binding species were largely removed from placental membranes by extensive washing and appeared in the supernate when membrane preparations were incubated at 4 C for 18 h, indicating that they were soluble and not an integral part of the particulate membrane; (3) limited proteolysis of placental membrane preparations did not result in the appearance of similar binding species; and (4) preparation of preterm placental membranes in the presence of protease inhibitors did not eliminate these binding species. Use of traditional methodology to study binding of somatomedin to receptors in membranes prepared from preterm human tissues may be misleading because of contamination by amniotic fluid and/or plasma-derived binding proteins.
Subject(s)
Carrier Proteins/isolation & purification , Fetal Proteins/isolation & purification , Fetus/metabolism , Receptors, Cell Surface/isolation & purification , Affinity Labels , Amniotic Fluid/analysis , Brain/embryology , Brain Chemistry , Cell Membrane/metabolism , Cross-Linking Reagents , Extraembryonic Membranes/metabolism , Female , Fetal Blood/metabolism , Gestational Age , Humans , Insulin-Like Growth Factor Binding Proteins , Molecular Weight , Placenta/metabolism , Pregnancy , Receptors, SomatomedinABSTRACT
A novel member of the Formin/Diaphanous family of proteins was cloned and characterized. A 4kB mRNA is ubiquitously expressed but is found in abundance in the spleen. FHOS (Formin Homologue Overexpressed in Spleen) contains a 3414bp open reading frame and encodes for an approximately 128kDa protein. FHOS has sequence homology to Diaphanous and Formin proteins within the Formin Homology (FH)1 and FH2 domains. FHOS also contains a coiled-coil, a collagen-like domain, two nuclear localization signals, and several potential PKC and PKA phosphorylation sites. FHOS-specific antiserum was generated and used to determine that FHOS is a predominantly cytoplasmic protein and is expressed in a variety of human cell lines. FHOS was mapped to chromosome 16q22 between framework markers WI-5594 and WI-9392.
Subject(s)
Fetal Proteins/chemistry , Fetal Proteins/isolation & purification , Nuclear Proteins/chemistry , Nuclear Proteins/isolation & purification , Sequence Homology, Amino Acid , Amino Acid Sequence , Blotting, Northern , Chromosome Mapping , Chromosomes, Human, Pair 16/genetics , Cloning, Molecular , Female , Fetal Proteins/biosynthesis , Formins , HL-60 Cells , Humans , Microfilament Proteins , Molecular Sequence Data , Nuclear Proteins/biosynthesis , RNA/analysis , Sequence Alignment , Spleen/metabolism , Tumor Cells, CulturedABSTRACT
Immunoadsorption is a simple and effective method for purifying antigens and antibodies. The major interfering effect is non-biospecific binding, especially when crude preparations of antibodies or antigens are coupled to the matrix. In this paper various possibilities for eliminating these effects are compared and analysed. It was found that for antigen (AFP) purification pre-elution with a pH 10.0 buffer of high ionic strength (1.0 M) is effective. A major problem in purifying antibodies in the non-biospecific binding of IgG. This can be eliminated by using a non-biospecific immunoadsorbent column, where specific antibodies are not, or only weakly bound and IgG with irrelevant specificity but exhibiting high non-biospecific interaction is retained. This has been applied to preparation of sheep anti-pepsinogen II group protease antibodies.
Subject(s)
Antibodies/isolation & purification , Antigens/isolation & purification , Fetal Proteins/isolation & purification , alpha-Fetoproteins/isolation & purification , Absorption , Chromatography, Affinity/methods , Humans , Immunoassay , Pepsinogens/immunology , Protein Binding , Sepharose , alpha-Fetoproteins/immunologyABSTRACT
An enzymoimmunoassay (EIA) for the quantitation of soluble antigens having at least two antibody-combining sites is descirbed. This non-competitive sandwich method comprises three steps: 1) the antigen to be assayed is reacted with the antibody-coated cellulose immunosorbent, 2) the enzyme-labelled antibody is then incubated with the antigen bound to the solid phase, 3) the enzymatic activity of the immunosorbent is then measured. This activity increases with the quantity of antigen to be assayed. Examples of the application of this method to the assay of rat and human alpha-fetoprotein (AFP) are given. When applied to rat and human AFP, this assay gives reproducible results in the range of 10--1000 ng/ml and 3-1000 ng/ml respectively. AFP sera concentrations of normal and pregnant rats were assayed by EIA, radioimmunoassay RIA and rocket-immunoelectrophoresis (RIE). In all the cases good agreement was noted among these three techniques. Rheumatoid factor, whenever present, may interfere with the assay. However, the effect of this interaction can be eliminated. The advantages of the EIA method can be listed as follows: a) no pure antigen is required, b) the final color reaction is developed from the solid phase. This feature eliminates most non-specifically interfering factors, c) the range of the assay covers a 2 log-scale, d) only inexpensive equipment is used, e) results are obtained within 24 hr, f) sensitivity and reproducibility lie within a range comparable to that of RIA.
Subject(s)
Fetal Proteins/isolation & purification , alpha-Fetoproteins/isolation & purification , Animals , Antibodies/isolation & purification , Binding Sites, Antibody , Chromatography, Thin Layer , Female , Glucose Oxidase/metabolism , Humans , Immune Sera/isolation & purification , Immunization , Immunoassay/methods , Immunoelectrophoresis , Pregnancy , Rabbits , Radioimmunoassay , Rats , Time Factors , alpha-Fetoproteins/analysis , alpha-Fetoproteins/immunologyABSTRACT
It was observed in the course of other studies that rat fetal lung extracts inhibited proliferation of fetal lung cells in culture. The purpose of the present study was to isolate and characterize this cytostatic factor. It was found that fetal lungs contained a 16 kDa cytostatic factor and its concentration was twofold greater in fetal lungs of diabetic rats compared with control rats. This fetal lung cytostatic protein (FLCP) was purified by reversed-phase, heparin-affinity and gel filtration high-performance liquid chromatography and SDS-PAGE. The purified protein was electroblotted onto polyvinylidene difluoride membrane and subjected to sequence analysis. The amino-terminal sequence of this fetal lung cytostatic protein was P E P A K S A P A P X K G I G K Q X X K A X X K A ... and showed significant homology with histone H2B; however, the amino acid composition of FLCP suggested that it may be structurally distinct from histone H2B. Ion-spray mass spectrometry suggested that FLCP was made up of at least two species of the protein with molecular weights of 13,776.1 and 14,007.3 and was different from the molecular weight of rat histone H2B predicted by its cDNA sequence. The concentration of FLCP, based on amino acid compositions, was 0.32 nmol/g and 0.83 nmol/g wet fetal lung from non-diabetic and diabetic rats respectively. These findings suggest that the fetal rat lung produces a regulatory factor bearing considerable homology with but possibly different from histone H2B and that fetal lung immaturity during diabetic pregnancy might be contributed to by an increase in this factor.
Subject(s)
Diabetes Mellitus, Experimental/metabolism , Fetal Proteins/isolation & purification , Lung/embryology , Amino Acid Sequence , Animals , Chromatography, High Pressure Liquid , Diabetes Mellitus, Experimental/embryology , Electrophoresis, Polyacrylamide Gel , Fetal Proteins/genetics , Histones/genetics , Lung/chemistry , Molecular Sequence Data , Rats , Rats, Sprague-Dawley , Sequence Homology, Amino AcidABSTRACT
By screening with monoclonal antibodies (mAbs) raised against growth cone membrane fraction from fetal porcine brains, we have identified a 230 kDa antigen, termed p230. Western blot analysis of extracts from various tissues demonstrated that p230 is specifically expressed in brains, in which its expression is temporally restricted; it was especially prominent in the embryonic and the early postnatal stage, and decreased to subdetectable levels in the adult brain. Further characterization of p230 revealed that it is a peripherally-membrane associated, cell surface protein produced by astrocytes. Neurite outgrowth of E18 rat cerebral cortex neurons cultured on a monolayer of astrocytes was significantly reduced in the presence of anti-p230 polyclonal antibody. Partial amino acid sequences of p230 purified from fetal porcine brains were highly homologous to an extracellular matrix protein, tenascin-C. These lines of evidence suggest that p230, a tenascin-C-like molecule present in fetal porcine brains, plays important roles during early brain development, particularly in growth cone guidance.
Subject(s)
Astrocytes/cytology , Brain/cytology , Brain/metabolism , Cerebral Cortex/cytology , Fetal Proteins/isolation & purification , Fetal Proteins/physiology , Nerve Tissue Proteins/isolation & purification , Nerve Tissue Proteins/physiology , Neurites/physiology , Neurons/cytology , Amino Acid Sequence , Animals , Antibodies, Monoclonal , Astrocytes/metabolism , Brain Chemistry , Cells, Cultured , Cerebral Cortex/metabolism , Embryo, Mammalian , Fetal Proteins/biosynthesis , Membrane Proteins/biosynthesis , Membrane Proteins/isolation & purification , Membrane Proteins/physiology , Mice , Mice, Inbred BALB C , Molecular Sequence Data , Nerve Tissue Proteins/biosynthesis , Neurites/metabolism , Neurons/metabolism , Rats , Rats, Wistar , Sensitivity and Specificity , Sequence Homology, Amino Acid , Swine , Tenascin/genetics , Tenascin/metabolismABSTRACT
Guinea-pig conceptuses obtained on Day 15 of pregnancy were cultured for 24 h in the presence of [3H]leucine. Proteins present in the culture medium were purified by dialysis, desalted, and subjected to analysis by gel filtration chromatography, ion-exchange chromatography, and polyacrylamide gel electrophoresis. The major non-radioactive protein present co-chromatographed with serum albumin. Fresh protein synthesis occurred as indicated by the incorporation of [3H]leucine, and the proteins produced were acidic in nature. Many radioactive proteins in relatively similar amounts were produced, with protein of molecular weights 28.8 and 98.2 kDa (as determined on Sephacryl S-300HR) and of molecular weights 8.4 and 14.7 kDa (as determined on Sephadex G-75SF) being synthesized in marginally greater quantities. Consequently, from the profile of proteins synthesized and secreted, no obvious candidate emerged as the anti-luteolytic factor synthesized and secreted by the guinea-pig conceptus during early pregnancy which inhibits endometrial PGF2 alpha synthesis and maintains corpus luteal function.
Subject(s)
Corpus Luteum/physiology , Embryo, Mammalian/metabolism , Fetal Proteins/biosynthesis , Animals , Chromatography, Gel , Chromatography, Ion Exchange , Corpus Luteum/drug effects , Culture Techniques , Electrophoresis, Polyacrylamide Gel , Female , Fetal Proteins/isolation & purification , Fetal Proteins/metabolism , Guinea Pigs , PregnancyABSTRACT
The co-culture of Day-15 guinea-pig conceptuses or Day-15 pregnant guinea-pig endometrium with Day-15 non-pregnant guinea-pig endometrium had no inhibitory effect on PGF2 alpha output from the non-pregnant endometrium. Unpurified proteins secreted by the Day-15 guinea-pig conceptuses, or these proteins purified by Blue Sepharose CL-6B and ion-exchange column chromatography also had no inhibitory effect on PGF2 alpha output from Day-15 non-pregnant guinea-pig endometrium cultured in vitro. However, following the further purification of guinea-pig conceptus secreted proteins on Sephadex G-75SF, the proteins present in fraction F3:4 inhibited PGF2 alpha output from the Day-15 non-pregnant guinea-pig endometrium during the first 6 h of culture. The major protein present in F3:4 had a molecular weight of 38.2 kDa on SDS-PAGE. Proteins present in F3:4 formed only a minor proportion of the total proteins secreted. Nevertheless, the anti-luteolytic factor secreted by the guinea-pig conceptus may be this 38.2 kDa protein, but further study is required.
Subject(s)
Embryo, Mammalian/metabolism , Endometrium/metabolism , Fetal Proteins/metabolism , Pregnancy Proteins/metabolism , Prostaglandins/metabolism , Animals , Culture Media , Culture Techniques , Embryo, Mammalian/chemistry , Embryo, Mammalian/physiology , Endometrium/drug effects , Endometrium/physiology , Estrus/metabolism , Estrus/physiology , Female , Fetal Proteins/isolation & purification , Fetal Proteins/physiology , Guinea Pigs , Pregnancy , Pregnancy Proteins/isolation & purification , Pregnancy Proteins/physiologyABSTRACT
Two alpha1-globulin bands of fetal serum with relative mobilities against bromophenol blue of 0.55 and 0.58 on 7% polyacrylamide gel electrophoresis reacted with a monospecific rabbit antiserum to alpha-fetoprotein (AFP). The former globulin band was clearly detected in the fetal liver supernatant. AFP was immunochemically purified from both the fetal serum and liver, and their electrophoretic and immunochemical properties were compared. Liver AFP purified by immunoadsorbent column yielded electrophoretic mobilities and relative amounts of the two electrophoretically distinct components identical with the purified serum AFP. The immunological reactivity of the two components of the purified preparations from serum and liver against the monospecific anti-AFP serum was also indistinguishable. After the removal of the sialic acid residues from purified serum and liver AFP by treatment with neuraminidase for 6 to 12 hr, disc electrophoretic patterns on 5% polyacrylamide gel and immunoelectrophoretic patterns of the treated AFP were found to be closely similar in both preparations. It may be possible to conclude that serum and liver AFP are structurally indistinguishable and probably identical.
Subject(s)
Fetal Blood/analysis , Fetal Proteins/isolation & purification , Liver/analysis , alpha-Fetoproteins/isolation & purification , Alpha-Globulins/analysis , Animals , Electrophoresis, Disc , Immunodiffusion , Immunoelectrophoresis , Rats , alpha-Fetoproteins/immunologyABSTRACT
A membrane hyaluronate-binding protein from cerebral cortex of human embryonic brain (22-24 weeks) was purified by affinity, ion exchange chromatographies and gel-filtration. While gel-filtration analysis the protein had Mm 250 kDa. Electrophoresis under reduction conditions in the presence of DS-Na revealed a major band with Mm 85 kDa and two minor binds with Mm 68 and 36 kDa. The isolated protein did not react with antibodies against known hyaluronate-binding and other proteins with similar mass. The results show that a new membrane hyaluronate-binding protein was isolated and purified from human embryonic brain.