ABSTRACT
STUDY QUESTION: When should 'not so rare' Leydig cell tumors (LCTs) of the testis be suspected, diagnosed, and treated? SUMMARY ANSWER: LCTs are more frequent than generally believed, are associated with male infertility, cryptorchidism and gynecomastia, and should be treated conservatively (in compliant patients) with active surveillance, which appears to be a safe alternative to surgical enucleation. WHAT IS KNOWN ALREADY: Increasing referrals for testicular imaging have led to an increase in findings of LCTs. The features and natural history of these tumors remain largely unknown, as the available studies are small and heterogeneous. LCTs were previously treated aggressively and follow-up data are lacking. STUDY DESIGN, SIZE, DURATION: A case-cohort study of consecutive patients diagnosed with LCTs over a 10-year period was prospectively enrolled from 2009 to 2018 and compared to matched cohorts of patients with seminomas or no testicular lesions screened in the same timeframe. PARTICIPANTS/MATERIALS, SETTING, METHODS: Of the 9949 inpatients and outpatients referred for scrotal ultrasound, a total of 83 men with LCTs were included. Enrolled subjects underwent medical history and clinical examination and were asked to undergo routine blood tests, hormone investigations (FSH, LH, total testosterone, estradiol, inhibin B, sex hormone-binding globulin (SHBG), prolactin), and semen analysis. Patients who consented also underwent contrast-enhanced ultrasound, elastography, gadolinium-enhanced scrotal magnetic resonance imaging, and hCG stimulation test (5000 IU i.m.) with serum total testosterone and estradiol measured at 0, 24, 48, and 72 hours. MAIN RESULTS AND THE ROLE OF CHANCE: In total, 83 patients diagnosed with LCTs were compared against 90 patients diagnosed with seminoma and 2683 patients without testicular lesions (NoL). LCTs were diagnosed by enucleation (48.2%), orchiectomy (13.3%), or clinical surveillance (38.5%). Testicular volume, sperm concentration, and morphology were lower (P = 0.001, P = 0.001, and P < 0.001, respectively) in patients with LCTs than in the NoL group. FSH, LH, and SHBG were higher and the testosterone/LH ratio was lower in LCTs than in the NoL group (P < 0.001). The LCT group showed higher SHBG (P = 0.018), lower sperm concentration (P = 0.029), and lower motility (P = 0.049) than the seminoma group. Risk factors for LCTs were cryptorchidism (χ2 = 28.27, P < 0.001), gynecomastia (χ2 = 54.22, P < 0.001), and low testicular volume (χ2 = 11.13, P = 0.001). Five cases were recurrences or bilateral lesions; none developed metastases during follow-up (median, 66 months). LIMITATIONS, REASONS FOR CAUTION: This study has some limitations. First, hCG and second-line diagnostic investigations were not available for all tumor patients. Second, ours is a referral center for infertility, thus a selection bias may have altered the baseline features of the LCT population. However, given that the comparison cohorts were also from the same center and had been managed with a similar protocol, we do not expect a significant effect. WIDER IMPLICATIONS OF THE FINDINGS: LCTs are strongly associated with male infertility, cryptorchidism, and gynecomastia, supporting the hypothesis that testicular dysgenesis syndrome plays a role in their development. Patients with LCTs are at a greater risk of endocrine and spermatogenesis abnormalities even when the tumor is resected, and thus require long-term follow-up and prompt efforts to preserve fertility after diagnosis.LCTs have a good oncological prognosis when recognized early, as tissue-sparing enucleation is curative and should replace orchiectomy. Conservative surgery and, in compliant patients, active surveillance through clinical and radiological follow-up are safe options, but require monitoring of testicular failure and recurrence. STUDY FUNDING/COMPETING INTEREST(S): The project was funded by the Ministry of University and Research Grant MIUR 2015ZTT5KB. There are no conflicts of interest. TRIAL REGISTRATION NUMBER: ALCeP trial (ClinicalTrials.gov Identifier: NCT01206270).
Subject(s)
Leydig Cell Tumor/diagnosis , Orchiectomy , Testicular Neoplasms/diagnosis , Testis/surgery , Adult , Case-Control Studies , Estradiol/blood , Follicle Stimulating Hormone/blood , Follow-Up Studies , Humans , Leydig Cell Tumor/blood , Leydig Cell Tumor/surgery , Luteinizing Hormone/blood , Male , Organ Size , Prospective Studies , Sex Hormone-Binding Globulin/metabolism , Testicular Neoplasms/blood , Testicular Neoplasms/surgery , Testis/diagnostic imaging , Testosterone/blood , Treatment Outcome , Ultrasonography , Young AdultABSTRACT
OBJECTIVE: To analyze the clinical, hormonal, anatomical, and molecular characteristics of Leydig cell tumors, a very rare cause of progressive hyperandrogenism in children. STUDY DESIGN: Description of a 9-year-old boy with isosexual precocious pseudopuberty, and of a 12-year-old girl with rapidly progressive virilization, both due to a pure Leydig cell tumor. Review of all cases of pediatric Leydig cell tumors published since 1999 (when the first somatic mutations of the luteinizing hormone receptor were described) and reporting hormonal and/or molecular data. RESULTS: Boys (n = 24) are younger than girls (n = 12) at diagnosis (median 6.5 vs 13.0 years, P = .04). Plasma gonadotrophins are more often completely suppressed in boys (6 cases) than in girls (2 cases). Pure Leydig cell tumors are exceedingly rare in girls (2 cases), who most often have Sertoli-Leydig tumors. These tumors affect either testis equally (11 left, 13 right) but occur more often in the left ovary (8 left, 3 right). Activating mutations of the alpha-subunit of the G(s) stimulatory protein have not been found in either boys or girls and activating mutations of the luteinizing hormone receptor have only been found in boys. CONCLUSIONS: Leydig cell tumors in children display clinical, hormonal, anatomical, and molecular sexual dimorphism.
Subject(s)
Leydig Cell Tumor/diagnosis , Ovarian Neoplasms/diagnosis , Testicular Neoplasms/diagnosis , Biomarkers/blood , Child , Female , Genetic Markers , Gonadotropins/blood , Humans , Leydig Cell Tumor/blood , Leydig Cell Tumor/genetics , Male , Ovarian Neoplasms/blood , Ovarian Neoplasms/genetics , Phenotype , Sex Factors , Testicular Neoplasms/blood , Testicular Neoplasms/geneticsABSTRACT
When test article-related testicular toxicity or Leydig cell tumors are identified in nonclinical studies, the measurement of circulating hormones such as luteinizing hormone, follicle-stimulating hormone, inhibin, testosterone, or prolactin is often considered in order to aid mechanistic investigations or to identify potential biomarkers in man. Although some hormone levels are relatively constant, others are subject to wide variability owing to pulsatility of secretion, diurnal rhythms, and stress. To avoid being misled, it is important that this variation is factored into any study design that includes hormone measurements. Since all these possibilities start from the pathologist's reading of the tissue sections, we begin with a review of the morphologic changes that are tied to underlying alterations in hormones. We then provide the reader with basic information and representative hormone data, including coefficients of variation, for the major male reproductive hormones in the three main nonclinical species (rats, dogs, and cynomolgus monkeys). Power and probability tables for rats and dogs allow estimates of the number of animals or samples needed to provide a given likelihood of detecting a hormonal change of a given size. More importantly, we highlight the variability of this process and the real value in readers developing this information at their own site.
Subject(s)
Biomarkers/blood , Leydig Cell Tumor/chemically induced , Testis/drug effects , Testosterone/blood , Toxicity Tests/methods , Xenobiotics/toxicity , Animals , Animals, Laboratory , Dogs , Follicle Stimulating Hormone/blood , Leydig Cell Tumor/blood , Luteinizing Hormone/blood , Macaca fascicularis , Male , Prolactin/blood , Rats , Research Design , Species Specificity , Testis/metabolismABSTRACT
Gynecomastia is a frequent sign that may be physiological or caused by various benign or malignant diseases. In rare cases, it may be caused by testicular tumors. We describe a case of progressive gynecomastia at age 20 due to a Leydig cell tumor of the right testicle in a patient with a previous history of left-sided cryptorchidism. The patient underwent orchidectomy and testicular prosthesis placement, with subsequent improvement of gynecomastia and normalization of estrogen. Our case, in addition to demonstrating that gynecomastia may regress if the underlying cause is treated in a timely manner, shows that cryptorchidism may be related with the development of Leydig cell tumors in the same way as it is in other testicular tumors.
A ginecomastia é um sinal frequente que pode ser fisiológica ou causada por várias doenças benignas ou malignas. Em casos raros pode ser originada por tumores testiculares. Nós descrevemos um caso de ginecomastia de início rapidamente progressivo aos 20 anos por um tumor de células de Leydig do testículo direito em doente com história pregressa de criptorquidia esquerda. O doente foi submetido a orquidectomia e colocação de prótese testicular assistindo-se a melhoria da ginecomastia e normalização dos valores de estrogénio. O nosso caso, além de demonstrar que a ginecomastia pode regredir se a causa subjacente for tratada atempadamente, mostra que a criptorquidia poderá estar associada ao aparecimento de tumores de células de Leydig à semelhança do que acontece com outros tumores testiculares.
Subject(s)
Cryptorchidism/surgery , Gynecomastia/etiology , Leydig Cell Tumor/surgery , Orchiectomy , Testicular Neoplasms/surgery , Testis/surgery , Estrogens/blood , Humans , Leydig Cell Tumor/blood , Leydig Cell Tumor/complications , Male , Scrotum/pathology , Testicular Neoplasms/blood , Testicular Neoplasms/complications , Testis/diagnostic imaging , Treatment Outcome , Young AdultSubject(s)
Alopecia/etiology , Leydig Cell Tumor/complications , Ovarian Neoplasms/complications , Virilism/etiology , Aged , Biomarkers, Tumor/blood , CA-125 Antigen/blood , Clitoris/pathology , Female , Hirsutism/etiology , Humans , Hypertrophy , Hysterectomy , Leiomyoma , Leydig Cell Tumor/blood , Leydig Cell Tumor/diagnosis , Leydig Cell Tumor/surgery , Membrane Proteins/blood , Neoplasms, Second Primary , Omentum/surgery , Ovarian Neoplasms/blood , Ovarian Neoplasms/diagnosis , Ovarian Neoplasms/surgery , Postmenopause , Testosterone/blood , Uterine Neoplasms , Virilism/bloodSubject(s)
Alopecia/etiology , Hirsutism/etiology , Leydig Cell Tumor/complications , Ovarian Neoplasms/complications , Adenoma/surgery , Adrenal Gland Neoplasms/surgery , Adrenalectomy , Aged , Alopecia/blood , Estradiol/blood , Female , Hirsutism/blood , Humans , Leydig Cell Tumor/blood , Leydig Cell Tumor/diagnosis , Leydig Cell Tumor/surgery , Neoplasms, Multiple Primary/surgery , Ovarian Neoplasms/blood , Ovarian Neoplasms/diagnosis , Ovarian Neoplasms/surgery , Ovariectomy , Postmenopause , Sex Hormone-Binding Globulin/analysis , Testosterone/bloodABSTRACT
A 64-year-old postmenopausal female patient presented with approximately 5 years of intermittent spotting, progressive hirsutism and significantly increased libido and clitoral hypersensitivity with spontaneous orgasms multiple times a day beginning a few months prior. Initial hormone work-up revealed elevated total serum testosterone, androstenedione and 17-hydroxyprogesterone. Luteinising hormone, follicle stimulating hormone, estradiol, dehydroepiandrosterone-sulfate, thyroid stimulating hormone and prolactin were all within normal limits. Initial suspicions suggested an androgen-secreting tumour, likely in the ovary. The lesion was undetectable on transvaginal ultrasound and abdominal-pelvic CT scan. Laparoscopic bilateral salpingo-oophorectomy was performed to remove the likely source of excess androgens. Visible gross lesions were not observed intraoperatively; however, bilateral Leydig (hilus cell) tumours were confirmed by histopathology. Serum testosterone, androstenedione and 17-hydroxyprogesterone levels were normalised postoperatively within 2 weeks and 1 month, respectively.
Subject(s)
Hirsutism/etiology , Leydig Cell Tumor/diagnosis , Ovarian Neoplasms/diagnosis , Ovary/pathology , 17-alpha-Hydroxyprogesterone/blood , Androstenedione/blood , Female , Humans , Leydig Cell Tumor/blood , Leydig Cell Tumor/complications , Leydig Cell Tumor/surgery , Middle Aged , Ovarian Neoplasms/blood , Ovarian Neoplasms/complications , Ovarian Neoplasms/surgery , Ovary/surgery , Postmenopause , Salpingo-oophorectomy , Testosterone/blood , Treatment OutcomeSubject(s)
Leydig Cell Tumor/secondary , Leydig Cell Tumor/therapy , Orchiectomy , Testicular Neoplasms/pathology , Testicular Neoplasms/therapy , Aged , Biomarkers, Tumor/blood , Biopsy , Humans , Leydig Cell Tumor/blood , Lymph Node Excision , Lymphatic Metastasis , Male , Mitotic Index , Neoplasm Invasiveness , Neoplasm Staging , Reoperation , Testicular Neoplasms/blood , Testosterone/blood , Time Factors , Tomography, X-Ray Computed , Treatment OutcomeABSTRACT
Leydig cell tumors of the ovary are very rare, frequently associated with symptoms of virilization in postmenopausal patients. It is sometimes difficult to localize the tumor precisely even with modern imaging techniques. A 62-year-old patient presented with recent onset of rapidly progressive virilization including increased hirsutism, progressive balding, deepening voice and enlargement of the clitoris. Initial laboratory examination revealed a total serum testosterone level of 1330 ng/dL. Serum dehydroepiandrosterone sulfate, androstenedione and 17 hydroxyprogesterone levels were all within normal limits. Extensive pre-operative evaluations included transvaginal ultrasound, abdominal computed tomography and magnetic resonance imaging failed to localize the tumor. Therefore, selective ovarian venous hormonal sampling (SOVHS) was performed and they revealed that the total serum testosterone level was significantly higher in the left than in the right ovarian vein (7000 ng/dL vs. 225 ng/dL). A total abdominal hysterectomy and bilateral salpingo-oophorectomy was performed. Microscopic examination of the left ovary revealed a Leydig cell tumor. In conclusion, when the precise location of the tumor is not determined pre-operatively, SOVHS may be valuable to make accurate diagnosis.
Subject(s)
Leydig Cell Tumor/blood , Ovarian Neoplasms/blood , Testosterone/blood , Female , Humans , Hysterectomy , Leydig Cell Tumor/pathology , Leydig Cell Tumor/surgery , Middle Aged , Ovarian Neoplasms/pathology , Ovarian Neoplasms/surgery , Ovary/blood supply , VeinsABSTRACT
AIM: We describe the clinical features of a 6-year-old boy with sexual precocity caused by a somatic activating mutation of the luteinizing hormone (LH) receptor gene preceding gonadotropin-releasing hormone (GnRH)-dependent sexual precocity. STUDY DESIGN: Genomic DNA was extracted from the right testis and from the peripheral leukocytes followed by DNA amplification and sequencing of the LH receptor gene. We described the clinical characteristics including anthropometric parameters, bone age, and endocrine evaluation when the boy presented with sexual precocity. These data were compared with the clinical and hormonal evaluation after orchiectomy preceding GnRH-dependent sexual precocity and after subsequent treatment with GnRH agonist. RESULTS: No mutation was found in the sequence of the LH receptor gene extracted from peripheral leukocytes. Interestingly, sequencing of the tumor LH receptor gene revealed a heterozygous mutation in exon 11 encoding a replacement of Asp(578)His. Despite normalization of plasma testosterone, true precocious puberty was triggered within a year. CONCLUSIONS: Inmales with GnRH-independent sexual precocity the presence of small testicular Leydig cell tumorous lesions harboring a somatic mutation of the LH receptor gene should be considered. A close follow-up of affected patients should be instigated in order to monitor recurrence or subsequent true precocity.
Subject(s)
Leydig Cell Tumor/genetics , Mutation, Missense , Neoplasm Proteins/genetics , Puberty, Precocious/genetics , Receptors, LH/genetics , Testicular Neoplasms/genetics , Amino Acid Substitution , Child , Exons/genetics , Gonadotropin-Releasing Hormone/blood , Heterozygote , Humans , Leydig Cell Tumor/blood , Leydig Cell Tumor/physiopathology , Male , Neoplasm Proteins/metabolism , Puberty, Precocious/blood , Puberty, Precocious/physiopathology , Receptors, LH/metabolism , Testicular Neoplasms/blood , Testicular Neoplasms/physiopathologyABSTRACT
Virilizing ovarian tumors are rare and can occur at any age. In postmenopausal women, they commonly present with signs of masculinization. These tumors should be suspected in any patient with virilization and high testosterone levels (>1ng/mL). Tumor localization is sometimes difficult. These tumors are usually benign; surgical resection is the accepted treatment. Masculinizing consequences of hormonal secretions may be managed by cosmetologic treatments which should not be overlooked.
Subject(s)
Leydig Cell Tumor/surgery , Ovarian Neoplasms/surgery , Postmenopause , Virilism/etiology , Aged , Aged, 80 and over , Alopecia/etiology , Female , Humans , Leydig Cell Tumor/blood , Leydig Cell Tumor/diagnosis , Middle Aged , Ovarian Neoplasms/blood , Ovarian Neoplasms/diagnosis , Ovariectomy , Testosterone/blood , Treatment Outcome , Virilism/bloodABSTRACT
We report a case of a benign testicular Leydig-Cell Tumor (LCT) that deceived us because of an estradiol (E2) plasma levels elevation 27 months after radical orchiectomy in a body builder patient with habits of red meat abuse and no steroid assumption, without any sign of tumor recurrence. The patient was therefore asked to stop red meat assumption and E2 plasma levels returned normal. The restoration of red meat assumption showed a trend of increasing E2 plasma levels above normal range. Despite the documented usefulness of E2 plasma levels evaluation during the follow-up of LCT, elevation of this hormone could be related to other causes and presence of the so-called evironmental xenoestrogens may be one of these.
Subject(s)
Estradiol/blood , Leydig Cell Tumor/blood , Neoplasm Recurrence, Local/blood , Testicular Neoplasms/blood , Adult , Follow-Up Studies , Humans , Male , Predictive Value of Tests , Reproducibility of ResultsABSTRACT
BACKGROUND: Clinical management of germ cell tumours (GCTs) relies on monitoring of serum tumour markers. However, the markers α-fetoprotein (AFP), the ß-subunit of human chorionic gonadotropin (bHCG), and lactate dehydrogenase (LDH) are expressed in <60% of GCT cases. OBJECTIVE: To test the utility of the microRNAs (miRNAs) miR-371a-3p, miR-372-3p, miR-373-3p, and miR-367-3p as sensitive and specific GCT serum biomarkers. DESIGN, SETTING, AND PARTICIPANTS: Serum levels of miRNAs were measured in 166 consecutive patients with GCT before and after treatment and in 106 male controls. In the first 50 consecutive patients, all four miRNAs were measured. In the main study, only the most sensitive miRNA was further analysed. OUTCOME MEASUREMENTS AND STATISTICAL ANALYSIS: The specificity and sensitivity of the four miRNAs were studied using receiver operating characteristic curves. miRNA sensitivities were compared to those of classical markers. Statistical cross-comparisons of miRNA levels for GCT subgroups and controls were performed at various time points during treatment. RESULTS AND LIMITATIONS: Overall, miR-371a-3p performed best, with 88.7% sensitivity (95% confidence interval [CI] 82.5-93.3%) and 93.4% specificity (95% CI 86.9-97.3%) and an area under the curve of 0.94, outperforming AFP, bHCG, and LDH (combined sensitivity 50%). According to Kernel density estimation, the sensitivity and specificity were 86.3% and 92.5%, respectively. miR-371a-3p levels dropped to normal after completion of treatment. The miRNA levels correlated with treatment failure and relapse. Teratoma did not express miR-371a-3p. CONCLUSIONS: The miRNA miR-371a-3p is a specific and sensitive novel serum GCT biomarker that accurately correlates with disease activity. Validation of this test in a large-scale prospective study is needed. PATIENT SUMMARY: miR-371a-3p is a novel serum marker for germ cell tumours that is expressed by 88.7% of patients and thus is far more sensitive and specific than classical serum markers. It correlates with tumour burden and treatment results. Validation in a large patient cohort is needed.
Subject(s)
Leydig Cell Tumor/blood , MicroRNAs/blood , Neoplasms, Germ Cell and Embryonal/blood , Testicular Neoplasms/blood , Adult , Biomarkers, Tumor/blood , Humans , Male , Middle Aged , Sensitivity and Specificity , Young AdultSubject(s)
Adrenal Glands/blood supply , Dehydroepiandrosterone Sulfate/blood , Hirsutism/etiology , Leydig Cell Tumor/blood , Ovarian Neoplasms/blood , Ovary/blood supply , Testosterone/blood , Veins , Virilism/etiology , Aged , Algorithms , Female , Humans , Leydig Cell Tumor/diagnosis , Leydig Cell Tumor/metabolism , Leydig Cell Tumor/surgery , Ovarian Neoplasms/diagnosis , Ovarian Neoplasms/metabolism , Ovarian Neoplasms/surgery , Ovariectomy , Testosterone/metabolism , Vena Cava, InferiorABSTRACT
Leydig cell tumors (LTC) are uncommon neoplasms arising from gonadal stroma that account for 1-3% of all testicular tumors. We report a case of LCT in a 36 years old man who had been suffering from painful bilateral gynecomastia for one year. Endocrine function tests showed decreased gonadotropin concentrations, and reduction of testosterone/estradiol ratio. Ultrasonography revealed a 10-12 mm hypoechoic area in the right testis, not evident on physical examination. Right orchiectomy was performed and histological examination confirmed the supposed existence of a LCT. After surgery, the gynecomastia has completely disappeared and hormonal alterations returned to normal.
Subject(s)
Gynecomastia/etiology , Leydig Cell Tumor/complications , Leydig Cell Tumor/diagnosis , Orchiectomy , Testicular Neoplasms/complications , Testicular Neoplasms/diagnosis , Adult , Estradiol/blood , Humans , Leydig Cell Tumor/blood , Leydig Cell Tumor/surgery , Male , Testicular Neoplasms/blood , Testicular Neoplasms/surgery , Testosterone/blood , Treatment OutcomeABSTRACT
Leydig cell tumors of the ovary account for less than 0.1% of all ovarian tumors (1). We present two cases in which patients had markedly elevated serum testosterone levels and frank hirsutism. Both cases revealed right ovarian Leydig cell tumors upon oophorectomy with post-surgical resolution of hypertestosteronemia. While rare and difficult to diagnose, androgen secreting tumors should be suspected in women with hyperandrogenism and hirsutism, especially in the postmenopausal population.
Subject(s)
Leydig Cell Tumor , Ovarian Neoplasms , Aged , Female , Hirsutism/etiology , Humans , Leydig Cell Tumor/blood , Leydig Cell Tumor/complications , Leydig Cell Tumor/diagnosis , Leydig Cell Tumor/surgery , Middle Aged , Ovarian Neoplasms/blood , Ovarian Neoplasms/complications , Ovarian Neoplasms/diagnosis , Ovarian Neoplasms/surgery , Postmenopause , Testosterone/bloodABSTRACT
A GnRH superagonist, buserelin, was administered for 16 days to a postmenopausal woman with a testosterone-secreting ovarian tumor. Serum gonadotropin levels decreased by more than 70%, and serum testosterone fell by more than 50%. This short term study demonstrates that in these uncommon tumors androgen secretion is gonadotropin sensitive, and suggests that GnRH analogs may have therapeutic value in such patients.
Subject(s)
Buserelin/therapeutic use , Leydig Cell Tumor/metabolism , Ovarian Neoplasms/metabolism , Pituitary Hormone-Releasing Hormones/therapeutic use , Testosterone/metabolism , Administration, Intranasal , Chorionic Gonadotropin , Female , Follicle Stimulating Hormone/blood , Humans , Leydig Cell Tumor/blood , Luteinizing Hormone/blood , Middle Aged , Ovarian Neoplasms/blood , Testosterone/bloodABSTRACT
The effect of chronic hyperestrogenism on gonadal function was studied in three men who had estrogen-secreting Leydig cell tumors before unilateral orchidectomy and for 11-43 months after surgery. All three men had low plasma gonadotropin and testosterone levels and increased estradiol levels. Impairment of testicular steroidogenesis was also suggested by increased progesterone to 17-hydroxyprogesterone and 17-hydroxyprogesterone to androstenedione ratios in both spermatic venous plasma and the medium of Leydig tumor cells from one patient incubated in vitro. Before surgery, spermatogenesis was abnormal in two men. Testicular endocrine function and spermatogenesis did not return to normal after surgery. During the follow-up period, plasma gonadotropin levels were high in all three men, and testosterone was low normal. Estradiol levels decreased to normal immediately after surgery and then returned to the upper normal limit. The response to hCG stimulation in one man was subnormal. We conclude that chronic hyperestrogenism produced hypothalamo-pituitary inhibition as well as direct steroidogenic blockade at the testicular level. Long term impairment of both endocrine and exocrine testicular functions may be secondary to slowly reversible (or irreversible) estrogen-induced damage to tubular and Leydig cells.
Subject(s)
Gonadal Steroid Hormones/blood , Leydig Cell Tumor/blood , Orchiectomy , Testicular Neoplasms/blood , Adult , Follow-Up Studies , Gonadotropins, Pituitary/blood , Gynecomastia/etiology , Humans , Leydig Cell Tumor/complications , Leydig Cell Tumor/surgery , Male , Testicular Neoplasms/complications , Testicular Neoplasms/surgeryABSTRACT
Ovarian virilizing tumors are rare and can lead to assessment difficulties because of their small size. A 41-yr-old female was referred for evaluation of hirsutism that had increased within the previous 3 yr. Menstrual cycle length was normal. Plasma testosterone was 3.9 ng/ml (normal range, 0.2-0.8 ng/ml), was not suppressible by 2 mg dexamethasone (4.3 ng/ml), and was increased (6.3 ng/ml) after three daily injections of hCG (5000 IU). Abdominal computed tomography scan showed an adrenal nodule (13 x 6 mm) that remained unchanged after 3 months. Ultrasound examination of the pelvis was normal. Ovarian and adrenal venous catheterization did not yield additional information. Topographic assessment was made by intraoperative measurement of testosterone in the samples taken from each ovarian vein (competitive chemiluminescent immunoassay ADVIA Centaur; right ovarian vein, 105 ng/ml; left ovarian vein, 5 ng/ml; peripheral blood, 7 ng/ml). Right annexectomy resulted in normalization of testosterone levels (0.22 ng/ml). Histopathological examination found a Leydig cell tumor of hilar type (1.5 cm). This observation illustrates the usefulness of intraoperative measurement of testosterone by a rapid automated technique for topographic assessment of ovarian virilizing tumor in premenopausal women.
Subject(s)
Hirsutism/etiology , Leydig Cell Tumor/complications , Leydig Cell Tumor/surgery , Ovarian Neoplasms/complications , Ovarian Neoplasms/surgery , Testosterone/blood , Adult , Female , Humans , Intraoperative Period , Leydig Cell Tumor/blood , Leydig Cell Tumor/pathology , Osmolar Concentration , Ovarian Neoplasms/blood , Ovarian Neoplasms/pathology , Polycystic Ovary Syndrome/complicationsABSTRACT
The recovery of exocrine and endocrine testicular function was studied in six patients orchidectomized for an estrogen-producing Leydig cell tumor. Gynecomastia disappeared in four patients. The contralateral testis, whose volume was reduced, returned to normal size after 30 days. Sperm density returned to normal in only one of the four patients in whom the preoperative sperm count was reduced. One day after unilateral orchidectomy, plasma estradiol decreased to normal and testosterone (T) fell about 50%. On the 10th postoperative day, plasma T [5.60 +/- 1.20 ng/ml (SD)] was normal. On day 120, T was higher than on day 10 (6.83 +/- 1.20 ng/ml). There was no significant increase of T after a single injection of hCG (5000 IU) on day 10, and the T response was similar to that of normal men on day 120. Plasma FSH and LH were increased on the 10th postoperative day; they then decreased between 60 and 120 days after the operation but were still above the normal values on day 120. The FSH/LH ratio, which was 0.43 +/- 0.17 preoperatively returned to normal (1.60 +/- 0.25) 10 days postoperatively. In conclusion, after hemicastration for an estrogen-secreting tumor, testicular hormonal secretion returns to normal within 120 days but spermatogenesis may still be impaired at this time.