A whole process study of dual microalgae cultivation coupled to domestic wastewater treatment and wheat growth.

The multiple microalgal collaborative treatment of domestic wastewater has been extensively investigated, but its whole life cycle tracking and consequent potential have not been fully explored. Herein, a dual microalgal system was employed for domestic wastewater treatment, tracking the variation in microalgal growth and pollutants removal from shake flask scale to 18 L photobioreactors scales. The results showed that Chlorella sp. HL and Scenedesmus sp. LX1 combination had superior growth and water purification performance, and the interspecies soluble algal products promoted their growth. Through microalgae mixing ratio and inoculum size optimized, the highest biomass yield (0.42 ± 0.03 g/L) and over 91 % N, P removal rates were achieved in 18 L photobioreactor. Harvested microalgae treated in different forms all promoted wheat growth and suppressed yellow leaf rate. This study provided data support for the whole process tracking of dual microalgal system in treating domestic wastewater and improving wheat growth.

Enhancing bioremediation potential of microalgae Chlorella vulgaris and Scenedesmus acutus by NaCl for pyrene degradation.

Microalgae are increasingly recognized as promising organisms for bioremediation of organic pollutants. This study investigates the potential of enhancing the bioremediation efficiency of pyrene (PYR), a polycyclic aromatic hydrocarbon (PAH), through NaCl induced physiological and biochemical alterations in two microalgae species, Chlorella vulgaris and Scenedesmus acutus. Our findings reveal significant improvement in PYR removal when these microalgae were cultivated in the presence of 0.1% NaCl where PYR removal increased from 54 to 74% for C. vulgaris and from 26 to 75% for S. acutus. However, it was observed that NaCl induced stress had varying effects on the two species. While C. vulgaris exhibited increased PYR removal, it experienced reduced growth and biomass production, as well as lower photosynthetic efficiency when exposed to PYR and PYR + NaCl. In contrast, S. acutus displayed better growth and biomass accumulation under PYR + NaCl conditions, making it a more efficient candidate for enhancing PYR bioremediation in the presence of NaCl. In addition to assessing growth and biochemical content, we also investigated stress biomarkers, such as lipid peroxidation, polyphenol and proline contents. These findings suggest that S. acutus holds promise as an alternative microalgae species for PYR removal in the presence of NaCl, offering potential advantages in terms of bioremediation efficiency and ecological sustainability. This study highlights the importance of understanding the physiological and biochemical responses of microalgae to environmental stressors, which can be harnessed to optimize bioremediation strategies for the removal of organic pollutants like PYR.

Self-flocculation behaviour of cellulose-based bioflocculant synthesized from sewage water grown Chlorella sorokiniana and Scenedesmus abundans.

The global energy crisis has spurred a shift from conventional to clean and sustainable energy sources. Biomass derived from microalgae is emerging as an alternative energy source with diverse applications. Despite the numerous advantages of microalgae, large-scale biomass harvesting is not economical and convenient. Self-flocculation is considered an effective phenomenon facilitated by extracting the flocculating substances from microalgae that assist aggregation of algal cells into flocs. A novel cellulose-based bioflocculant has been synthesized from sewage water grown Chlorella sorokiniana and Scenedesmus abundans for harvesting application. The produced bioflocculant amounted to 38.5% and 19.38% of the dry weight of S. abundans and C. sorokiniana, respectively. Analysis via FTIR, XRD, and FESEM-EDX revealed the presence of cellulose hydroxyapatite (HA) in algae-derived cellulose. Harvesting efficiencies of 95.3% and 89.16% were attained for S. abundans and C. sorokiniana, respectively, at a dosage of 0.5 g/L. Furthermore, the bioflocculant was recovered, enabling its reuse with recovery efficiencies of 52% and 10% for S. abundans and C. sorokiniana, respectively. This simple and efficient approach has the potential to replace other harvesting methods, thereby contributing to the economic algal biofuel production.

Antimicrobial and Antioxidant Potential of Scenedesmus obliquus Microalgae in the Context of Integral Biorefinery Concept.

Small-scale photobioreactors (PBRs) in the inoculum stage were designed with internal (red or green) and external white LED light as an initial step of a larger-scale installation aimed at fulfilling the integral biorefinery concept for maximum utilization of microalgal biomass in a multifunctional laboratory. The specific growth rate of Scenedesmus obliquus (Turpin) Kützing biomass for given cultural conditions was analyzed by using MAPLE software. For the determination of total polyphenols, flavonoids, chlorophyll "a" and "b", carotenoids and lipids, UHPLC-HRMS, ISO-20776/1, ISO-10993-5 and CUPRAC tests were carried out. Under red light growing, a higher content of polyphenols was found, while the green light favoured the flavonoid accumulation in the biomass. Chlorophylls, carotenoids and lipids were in the same order of magnitude in both samples. The dichloromethane extracts obtained from the biomass of each PBR synergistically potentiated at low concentrations (0.01-0.05 mg/mL) the antibacterial activity of penicillin, fluoroquinolones or oregano essential oil against the selected food-borne pathogens (Staphylococcus aureus, Escherichia coli and Salmonella typhimurium) without showing any in vitro cytotoxicity. Both extracts exhibited good cupric ion-reducing antioxidant capacity at concentrations above 0.042-0.08 mg/mL. The UHPLC-HRMS analysis revealed that both extracts contained long chain fatty acids and carotenoids thus explaining their antibacterial and antioxidant potential. The applied engineering approach showed a great potential to modify microalgae metabolism for the synthesis of target compounds by S. obliquus with capacity for the development of health-promoting nutraceuticals for poultry farming.

Enhancement of microalgae growth using magnetic artificial cilia.

Microalgae have shown great potential as a source of biofuels, food, and other bioproducts. More recently, microfluidic devices have been employed in microalgae-related studies. However, at small fluid volumes, the options for controlling flow conditions are more limited and mixing becomes largely reliant on diffusion. In this study, we fabricated magnetic artificial cilia (MAC) and implemented them in millimeter scale culture wells and conducted growth experiments with Scenedesmus subspicatus while actuating the MAC in a rotating magnetic field to create flow and mixing. In addition, surface of MAC was made hydrophilic using plasma treatment and its effect on growth was compared with untreated, hydrophobic MAC. The experiments showed that the growth was enhanced by ten and two times with hydrophobic and hydrophilic MAC, respectively, compared with control groups which contain no MAC. This technique can be used to investigate mixing and flow in small sample volumes, and the enhancement in growth can be beneficial for the throughput of screening studies. Moreover, the methods used for creating and controlling MAC can be easily adopted in labs without microfabrication infrastructures, and they can be mastered by people with little prior experience in microfluidics.

Indirect regulation of temperature in raceway reactors by optimal management of culture depth.

Temperature and irradiance are the two most relevant factors determining the performance of microalgae cultures in open raceway reactors. Moreover, inadequate temperature strongly reduces the biomass productivity in these systems even if enough sunlight is available. Controlling the temperature in large open raceway reactors is considered unaffordable because of the large amount of energy required. This study presents an indirect method for temperature regulation in microalgae raceway reactors by optimizing the culture depth. First, the effect of the culture depth on the raceway temperature is analyzed for different seasons of the year. Afterward, a simulation study is presented where the proposed control approach is compared to the normal operation mode with constant volume in the reactor. This study is also extended to industrial scale. Relevant improvements on the temperature factor and biomass production are presented. The developed knowledge allows the improvement of the performance in open raceway reactors up to 12% without involving additional energy and costs, being a suitable solution for large industrial reactors that until now have no options for controlling the temperature.

The effects of exogenous cerium on photosystem II as probed by in vivo chlorophyll fluorescence and lipid production of Scenedesmus obliquus XJ002.

Cerium is the most abundant rare earth metal in the earth's crust, and it has deleterious effects on aquatic ecosystems from fertilizer runoff. Scenedesmus obliquus is an oil-rich microalga that grows rapidly and is sensitive to many kinds of toxins. Given that microalgae are useful indicators of eutrophication and toxic stress, it was found that lower concentrations of cerium (0.50-5.00 mg·L-1 ) stimulated algal growth and increased chlorophyll a content, whereas higher concentrations (above 50.00 mg·L-1 ) had an inhibitory effect on algal growth and chlorophyll a content. The algal growth rate and chlorophyll a content peaked at a cerium concentration of 5.00 mg·L-1 . Both the donor and acceptor sides of photosystem II (PSII) reaction centers were sensitive to cerium-induced stress. Specifically, high concentrations of cerium damaged the oxygen evolving complex and PSII reaction center and suppressed electron transport at the donor and receptor side of the reaction center, influencing the absorption, transfer, and application of light energy in S. obliquus XJ002. In addition, we established a simple method to quantify the intracellular lipid content of S. obliquus XJ002, and the optimum staining conditions for Nile red were as follows: volume percentage of dimethyl sulfoxide was 2%, the concentration of Nile red was 2.0 µg·mL-1 , and the staining time of Nile red was 5 min. The addition of cerium resulted in a significant increase in the total lipid content of XJ002. When the concentration of cerium was 50 mg·L-1 , the total lipid content was 16.26% higher than the control group. This information will enhance our ability to utilize microelement fertilizer in biomass accumulation programs and will help to further reveal the key regulatory factors in the lipid metabolism, and would lay the foundation for promoting the research of microalgae bioenergy.

Shear stress computation in a millimeter thin flat panel photobioreactor: Numerical design validated by experiments.

Flat panels are the most spread type of photobioreactors for studying light effects on a microalgae culture. Their low thickness, usually between 1 and 3 cm, aims at ensuring light homogeneity across the culture. Yet because optical density has to remain very low, studies are still limited to low cell density cultures. To alleviate this problem, even thinner photobioreactors can be designed. Nevertheless, thin flat panel reactors are very prone to induce high shear stress. This work aimed at designing a new millimeter thin panel photobioreactor to study light effects on Chlorella and Scenedesmus genera. We proposed a numerical workflow that is capable of assessing the shear stress intensity in such a reactor. The numerical predictions were validated at three different levels: 2D preliminary simulations were able to reproduce bubble commonly known behaviors; close to the nozzle, the predictions were successfully confronted to shadowgraphy experimental reference; at the mini bioreactor scale, experimental and numerical mixing were found to be close. After these throughout validations, shear stress intensity in the photobioreactor was calculated over 1000 Lagrangian tracers. The experienced shear stress was agglomerated at the population level. From the computed shear stress, it was possible to choose the minimal reactor thickness that would not hinder cell growth.

Enhancing CO2 utilization by a physical absorption-based technique in microalgae culture.

Carbon dioxide supplementation is significant for cell growth in autotrophic cultures of microalgae. However, the CO2 utilization efficiency is quite low in most processes. Aimed at this problem, six kinds of physical absorption enhancers were investigated to enhance the biological carbon sequestration of microalgae. By the addition of a small amount of CO2 absorption enhancer, the total inorganic carbon concentration of the medium was significantly increased. In addition, the biomass productivity of Scenedesmus dimorphus was maximally increased by 63% by the addition of propylene carbonate in flask cultures. In cultures using an air-lift photobioreactor equipped with a pH-feedback control system to supply CO2, the CO2 consumption was maximally reduced by 71% with added polyethylene glycol dimethyl ether. This study indicates that the incorporation of physical absorption enhancers could be a promising approach to overcome the problems of low CO2 utilization efficiency and high carbon source cost in algal biomass production.

Mixotrophic growth regime as a strategy to develop microalgal bioprocess from nutrimental composition of tequila vinasses.

The selection of a suitable growth regime can increase the physiological performance of microalgae and improve bioprocess based on these microorganisms from agro-industrial residues. Thus, this study assessed the biotechnology capacity-biomass production, biochemical composition, and nutrient uptake-from tequila vinasses (TVs) as the nutrient source of three indigenous microalgae-Chlorella sp., Scenedesmus sp., and Chlamydomonas sp.-cultured under heterotrophic and mixotrophic conditions. The results demonstrated that under the mixotrophic regime, the three microalgae evaluated reached the highest nitrogen uptake, biomass production, and cell compound accumulation. Under this condition, Chlorella sp. and Scenedesmus sp. showed the highest nutrient uptake and biomass production, 1.7 ± 0.3 and 1.9 ± 0.3 g L-1, respectively; however, the biochemical composition, mainly carbohydrates and proteins, varied depending on the microalgal strain and its growth regime. Overall, our results demonstrated the biotechnological capacity of native microalgae from TVs, which may vary not only depending on the microalgal strain but also the culture strategy implemented and the characteristics of the residue used, highlighting-from a perspective of circular bio-economy-the feasibility of implementing microalgal bioprocess to reuse and valorize the nutrimental composition of TVs through biomass and high-valuable metabolite production, depicting a sustainable strategy for tequila agro-industry in Mexico.

Effect of olive mill wastewaters on Scenedesmus sp. growth, metabolism and polyphenols removal.

BACKGROUND: The three-phase extraction process of olive oil produces highly contaminated wastewater (OMW). The elimination of this toxic by-product is an important environmental issue that requires the development of an appropriate management solution. The cultivation of microalgae using OMW as growth medium was therefore studied using single (the culture medium was formed by 0% to 80% ultrafiltered olive mill wastewater (OMUF) or OMW added to BG11) and two-stage strategies (microalgae were firstly cultivated in the BG11 medium. In the second stage, 40% and 80% of OMUF and OMW were added to the culture). In this work, biodegradation of OMW and subsequent extraction of lipid and antioxidant molecules was investigated as an ecofriendly method for the bioremediation and valorization of OMW. RESULTS: For two-stage cultivation, OMUF and OMW stress enhanced the intracellular amount of polyphenol accumulated in Scenedesmus sp. and exhibited the highest 2, 2-diphenyl-1- picrylhydrazyl radical (DPPH) and 2,2'-azino-bis (3-ethylbenzoline-6-sulfonate) radical (ABTS) scavenging ability compared with single-stage cultivation. Moreover, the lipid profile is dominated by polyunsaturated acids. In the single-stage cultivation, the Ch a, Ch b, carotenoid, carbohydrate and lipid content of 2.57, 7.4, 1.69, 368, and 644 g kg-1 were observed in 40% OMUF added culture, respectively, along with high biomass productivity and 58% of polyphenol removal. Moreover, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay showed that the biomass of Scenedesmus sp. cultured on 40% OMUF did not show any toxic effect, making it an efficient strategy. CONCLUSION: The results indicate that Scenedesmus sp. is a promising microalga for the biotreatment of OMW and the extraction of bioactive metabolites. © 2021 Society of Chemical Industry.

Effect of Salinity Stress on Lipid Accumulation in Scenedesmus sp. and Chlorella sp.: Feasibility of Stepwise Culturing.

The enhanced lipid accumulation in microalgae is envisioned under special stress conditions with the cost of algal growth, which in turn affects the overall lipid productivity. The selection of suitable stress conditions facilitates better lipid productivity without any harmful effect on microalgae growth and algal biomass production. In the present study, we have attempted to select the best salinity conditions towards better growth, biomass accumulation, and lipid productivity of microalgae. The study also envisaged testing the feasibility of the stepwise salinity stress-induced cultivation approach to minimize the growth penalty effect of microalgae. The highest specific growth rate (0.129, 0.133, 0.113 µday-1) and doubling per day (0.185, 0.193, 0.163 per day) were obtained at salinity concentration of 40 mM NaCl in BG-11 medium for Scenedesmus quadricauda (Sq19), Scenedesmus dimorphus (Sd12), and Chlorella sp. (Chl16), respectively. Maximal lipid content of 18.28, 30.70, and 32.19%, and lipid productivity of 8.59, 13.81, and 10.27 mg l-1 day-1 were achieved at 160 mM of NaCl in BG-11 media with the Sq19, Sd12, and Chl16 algal isolates, respectively. The utilization of stepwise salinity stress (160 mM) induced cultivation of Sd12 algal isolate results in higher lipid content (39.42%) and slightly improved lipid productivity than the control (without any stress, 20.4% lipid content). The results indicate the feasibility of enhancing the lipid content and productivity through the salinity-induced stepwise cultivation strategy.

Effects and possible mechanisms of sanguinarine on the competition between Raphidiopsis raciborskii (Cyanophyta) and Scenedesmus obliquus (Chlorophyta): A comparative toxicological study.

The phytogenic algicide sanguinarine shows strong inhibitory effects on some bloom-forming cyanobacteria and exhibits great potential in cyanobacterial bloom mitigation. To evaluate the possible ecological effects of sanguinarine on microalgae, the effects and possible mechanisms of sanguinarine on the competition between bloom-forming cyanobacterium Raphidiopsis raciborskii (formerly named Cylindrospermopsis raciborskii) and green alga Scenedesmus obliquus were investigated through co-culture competition test and comparative toxicological study including growth characteristics, chlorophyll fluorescence transients, activities of antioxidant enzymes, and lipid peroxidation. The results of Raphidiopsis-Scenedesmus co-culture competition test showed that sanguinarine decreased the competition ability of R. raciborskii, which benefitted S. obliquus in winning the competition. Toxicological studies have shown that sanguinarine exhibited high inhibitory effects on the growth and photosynthesis of R. raciborskii but no obvious toxicity on S. obliquus at concentrations of no more than 80 µg L-1. Oxidative damage partially contributed but was not the primary mechanism for the toxicity of sanguinarine on R. raciborskii. The results presented in this study indicate that sanguinarine may be a good algicidal candidate in mitigation of Raphidiopsis-based water bloom.

A comprehensive study on the effect of light quality imparted by light-emitting diodes (LEDs) on the physiological and biochemical properties of the microalgal consortia of Chlorella variabilis and Scenedesmus obliquus cultivated in dairy wastewater.

The effect of light wavelengths on the physiological, biochemical and lutein content of the microalgal consortia Chlorella variabilis and Scenedesmus obliquus was evaluated using different light sources. Among different light treatments, cool-white fluorescent light produced the highest biomass of 673 mg L-1 with a specific growth rate of 0.75 day-1 followed by blue (500 mg L-1; 0.73 day-1). The chlorophyll content was enhanced under blue light (10.7 mg L-1) followed by cool fluorescent light (9.3 mg L-1), whereas the lutein productivity was enhanced under cool fluorescent light (7.22 mg g-1). Protein content of the microalgal consortia was enhanced under all light treatments with the highest protein accumulation under cool-white fluorescent light (~56% of dry mass) closely followed by amber light (52% of dry mass), whereas the carbohydrate content was higher under amber light (~35% of dry mass). The results revealed that the consortia could grow well on diluted dairy wastewater thereby reducing the cost of algal production when compared with the use of inorganic media and a two-phase culture process utilizing cool fluorescent and amber light could be employed for maximizing algal biomass and nutrient composition with enhanced lutein production. The study also emphasizes on the economic efficiency of LED lights in terms of biomass produced based on the modest electricity consumed and the importance of using amber light for cultivating microalgae for its nutrient content which has seldom been studied.

Comparative life cycle assessment of autotrophic cultivation of Scenedesmus dimorphus in raceway pond coupled to biodiesel and biogas production.

Life cycle assessment (LCA) of indigenous freshwater microalgae, Scenedesmus dimorphus, cultivation in open raceway pond and its conversion to biodiesel and biogas were carried out. The LCA inventory inputs for the biogas scenario was entirely based on primary data obtained from algal cultivation (in pilot scale raceway pond), harvesting, and biogas production; while only the downstream processing involved in biodiesel production namely drying, reaction and purification were based on secondary data. Overall, eight scenarios were modeled for the integrated process involving: algae-based CO2 capture and downstream processing scenarios for biodiesel and biogas along with impact assessment of nutrient addition and extent of recycling in a life cycle perspective. The LCA results indicated a huge energy deficit and net CO2 negative in terms of CO2 capture for both the biodiesel and biogas scenarios, majorly due to lower algal biomass productivity and higher energy requirements for culture mixing. The sensitivity analysis indicated that variability in the biomass productivity has predominant effect on the primary energy demand and global warming potential (GWP, kg CO2 eq.) followed by specific energy consumption for mixing algal culture. Furthermore, the LCA results indicated that biogas conversion route from microalgae was more energy efficient and sustainable than the biodiesel route. The overall findings of the study suggested that microalgae-mediated CO2 capture and conversion to biodiesel and biogas production can be energy efficient at higher biomass productivity (> 10 g m-2 day-1) and via employing energy-efficient systems for culture mixing (< 2 W m-3).

Alternative fertilizer-based growth media support high lipid contents without growth impairment in Scenedesmus obliquus BR003.

Nitrogen (N) sources have been target in microalgae cultivation studies, considering their nutritional impact on growth and high costs. Here, we have evaluated the growth of Scenedesmus obliquus BR003, applying alternative low-cost culture media containing ammonium and urea, or combinations of both N sources. The culture media were applied for indoor and outdoor cultivation, followed by growth analyses and metabolic characterization. The alternative culture media B4 and L4 supported higher biomass production (1.4 g L-1) compared to BG11 (nitrate-based medium). In addition, the lipid percentage was higher for B4 (ammonium-based culture medium), reaching up to 25% DW. High contents of carbohydrates (60%) and proteins (40%) were also obtained in media with ammonium and urea, respectively. Considering the lower costs of alternative fertilizer-based media, using ammonium and/or urea as N sources, and the high lipid content observed, we suggest these media as viable for large-scale production of S. obliquus.

Effects of aqueous extracts from the rhizome of Pontederia cordata on the growth and interspecific competition of two algal species.

Single and co-culture systems of Microcystis aeruginosa and Scenedesmus obliquus were prepared with different initial algal densities and treated with different concentrations of aqueous extracts from the rhizome of Pontederia cordata to study its inhibitory effect on algal growth and the competitive relationship between these two algal species. The results showed that aqueous extracts could inhibit the growth of M. aeruginosa and S. obliquus, and the inhibition rate of aqueous extracts on the growth of M. aeruginosa was always higher than that of S. obliquus. A Lotka-Volterra competition model revealed that these two algal species can co-exist without the addition of aqueous extracts, and S. obliquus exhibited a stronger ability to compete than that of M. aeruginosa. Meanwhile, the dominant algal species changed with the addition of aqueous extracts regardless of the initial ratios of the two algae. The species ratio of mixed cultures had a strong effect on the interspecific interaction between the two algae. The higher proportion of S. obliquus in the initial proportion of two algae, the stronger competitive ability of S. obliquus when compared with that of M. aeruginosa.

Nitrogen-dependent metabolic regulation of lipid production in microalga Scenedesmus vacuolatus.

Microalga Scenedesmus vacuolatus exhibited maximum growth, protein and carbohydrate contents at 10.0 mM concentration of nitrate, 1.0 mM of glutamate nitrogen and at C/N ratio (12 mM acetate+10 mM nitrate). However, these cell constituents showed the highest values in the C+N grown cells, but the lipid content was found to be the highest glutamate grown cells. FTIR analysis of Lipid/Carbohydrate and Lipid/Protein ratio and flow cytometric analysis of neutral lipids revealed higher lipid content in the glutamate grown cells than in the nitrogen starved, nitrate and C+N grown cells. The nitrate reductase activity was the highest in the C+ N grown cells and the lowest activity was found in the glutamate grown cells. A corollary of these results suggested that suppression of nitrogen assimilatory system, whether by glutamate or by nitrogen deprivation, was the most suitable physiological condition for enhanced lipid synthesis and biofuel production in microalgal cells.

Effect of algal surface area and species interactions in toxicity testing bioassays.

Single and multispecies algal bioassays were assessed using copper toxicity with three green algae (Scenedesmus subspicatus, Scenedesmus quadricauda and Ankistrodesmus angustus) and one blue-green algae species (Oscillatoria prolifera). Single and multispecies toxicity tests were conducted based on cell density as per standard toxicity testing, and on equivalent surface area. A higher copper sulfate toxicity was registered for O. prolifera, followed by S. subspicatus, S. quadricauda, and A. angustus in single-species toxicity tests based on cell density. Single species toxicity tests based on surface area showed increased copper toxicity with increasing algal surface area except for A. angustus. In multispecies control bioassays, the growth of A. angustus was inhibited in the presence of other species in surface area-based tests. As compared to single species bioassays, O. prolifera, and S. quadricauda showed a decreased sensitivity to copper sulfate in both cell density and surface area based multispecies tests. However, for the algae species with the smallest surface area, S. subspicatus, 96h-EC50 value decreased in multispecies bioassays based on surface area as compared to the single species test, while it increased in multispecies bioassays based on cell density. The difference in S. subspicatus sensitivity to copper between tests based on cell density and surface area supports the need to adopt multispecies toxicity testing based on surface area to avoid the confounding effect on copper toxicity of increased biomass for metal binding. 96h-EC50 values for all species combined in the multispecies test based on cell density and on surface area were significantly different from 96h-EC50 values obtained in single species bioassays. These results demonstrate that single-species bioassays may over- or underestimate metal toxicity in natural waters.

Evaluation of the novel nanoparticle material - CdSe quantum dots on Chlorella pyrenoidosa and Scenedesmus obliquus: Concentration-time-dependent responses.

Quantum dots (QDs), as a kind of novel nanomaterial, have the extensive applications in various fields, inevitably leading to increasing risks for the ecological environment. The mobilization of cadmium including metal smelting and subsequent machining for multifarious applications has caused the release of cadmium element into the environment. In this study, we evaluated the potential toxicity of a novel nanoparticle material CdSe QDs, using two green algae Chlorella pyrenoidosa and Scenedesmus obliquus. The impact of CdSe QDs and cadmium ions on algae and the sensitivity of the two algae on target compounds were also considered and compared. Our results showed the algal growth rates and chlorophyll content decreased with increasing exposure concentrations and durations. Moreover, the glutathione levels were decreased while the activities of superoxide dismutase increased, exhibiting their pivotal functions in defeating toxic stress. The increment of malondialdehyde levels revealed that the stresses of CdSe QDs and cadmium ions were contributed to the occurrence of oxidative damage. Our study also indicated that the impact of CdSe QDs was stronger than that of cadmium nitrate and the algal response was also species-specific. In addition, the TEM photographs of the algal ultrastructure showed the presence of surface attachment and uptake of QDs.