RESUMEN
Between 2011 and 2013, three breeders of variegated squirrels (Sciurus variegatoides) had encephalitis with similar clinical signs and died 2 to 4 months after onset of the clinical symptoms. With the use of a metagenomic approach that incorporated next-generation sequencing and real-time reverse-transcriptase quantitative polymerase chain reaction (RT-qPCR), the presence of a previously unknown bornavirus was detected in a contact squirrel and in brain samples from the three patients. Phylogenetic analyses showed that this virus, tentatively named variegated squirrel 1 bornavirus (VSBV-1), forms a lineage separate from that of the known bornavirus species. (Funded by the Federal Ministry of Food and Agriculture [Germany] and others.).
Asunto(s)
Bornaviridae/genética , Encéfalo/patología , Encefalitis Viral/virología , Infecciones por Mononegavirales/virología , Sciuridae/virología , Anciano , Animales , Anticuerpos Antivirales/sangre , Anticuerpos Antivirales/líquido cefalorraquídeo , Bornaviridae/clasificación , Bornaviridae/aislamiento & purificación , Encefalitis Viral/patología , Resultado Fatal , Humanos , Imagen por Resonancia Magnética , Masculino , Persona de Mediana Edad , Infecciones por Mononegavirales/patología , Infecciones por Mononegavirales/transmisión , Filogenia , ARN Viral , Análisis de Secuencia de ADN , Zoonosis/transmisión , Zoonosis/virologíaRESUMEN
In 2013, an adult red squirrel (Sciurus vulgaris) was found dead showing, during necropsy, acute diffuse catarrhal enteritis. Using a virus screening pipeline based on a pan-viral DNA microarray with downstream next-generation sequencing a novel squirrel-associated virus was identified with mastadenovirus-typical sequence elements. Phylogenetic analysis of hexon protein amino acid sequences demonstrated the highest similarity to Equine adenovirus (AdV) 2, but a strong divergence to a squirrel AdV from Korea and other rodent AdVs. Shorter hexon gene segment investigations confirmed a close relationship with other squirrel-derived AdVs from Europe. The novel virus species was tentatively designated as Squirrel Adenovirus-1.
Asunto(s)
Infecciones por Adenoviridae/veterinaria , Adenoviridae/aislamiento & purificación , ADN Viral/genética , Secuenciación de Nucleótidos de Alto Rendimiento/métodos , Análisis de Secuencia por Matrices de Oligonucleótidos , Sciuridae/virología , Adenoviridae/genética , Infecciones por Adenoviridae/virología , Animales , FilogeniaRESUMEN
A brain sample of a Natterer's bat tested positive for rabies with classical virological techniques. Molecular techniques confirmed the presence of Bokeloh bat lyssavirus (BBLV) in Germany for the second time. Sequence analysis revealed a close genetic relationship to the initial German BBLV case. Using a TaqMan RT-PCR specific for BBLV viral RNA was detected in various other organs albeit with differences in the relative viral load.