Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 63
Filtrar
Más filtros

País/Región como asunto
Tipo del documento
Intervalo de año de publicación
1.
Acta Endocrinol (Buchar) ; 13(4): 437-440, 2017.
Artículo en Inglés | MEDLINE | ID: mdl-31149213

RESUMEN

CONTEXT: It is unclear whether treatment is necessary for transient moderate hypocalcaemia occurring after total thyroidectomy; if it is present, it is unclear which treatment modality should be preferred. OBJECTIVE: To investigate both the necessity and effectiveness of different treatment approaches of oral and/or intravenous calcium treatment in patients with transient, postoperative, moderate hypocalcaemia. DESIGN: This is a case control study made between June 2014 and June 2015. SUBJECTS AND METHODS: Forty-five patients who had serum calcium levels 6 hours after total thyroidectomy between 7.5-8 mg/dL were divided into three equal groups: an oral calcium administration group, an intravenous calcium administration group and a no-treatment group. Serum calcium and parathyroid hormone levels were measured preoperatively and on postoperative days 1, 2, 5 and 10. RESULTS: For post-thyroidectomy patients with serum calcium 7.5-8 mg/dL in the early postoperative period, no significant difference in serum calcium or parathyroid hormone was detected between groups. CONCLUSIONS: Follow-up without treatment seems to be the most effective approach for moderate hypocalcaemia occurring in the early period following total thyroidectomy; this suggests that intravenous treatment should be avoided.

2.
West Indian Med J ; 64(2): 71-5, 2015 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-26360676

RESUMEN

OBJECTIVE: One of the most important factors playing a role in chronic hepatitis B pathogenesis is cytokine release and one of the cytokines with anti-inflammatory characteristic is interleukin-10 (IL-10). The aim of the present study is to examine IL-10 levels in patients with chronic hepatitis B. SUBJECTS AND METHODS: Sixty-three patients with chronic hepatitis B disease who had not received any antiviral treatment were included in the study. Serum IL-10 level was investigated by enzyme-linked immunosorbent assay (ELISA) method. In the control group, 25 healthy individuals with mean age similar to the patient population were included. Control and patient groups were compared and data were statistically analysed. RESULTS: Interleukin-10 levels of 25 patients with hepatitis B virus (HBV) DNA levels between 2000 and 20 000 IU/mL were compared with those of 25 subjects in the control group, and the level in the chronic hepatitis B group was statistically significantly higher (p < 0.05). Interleukin-10 levels of 38 patients with HBV DNA > 20 000 IU/mL were statistically significantly higher than those in the control group. When chronic hepatitis B patients were compared among themselves, IL-10 levels increased as HBV DNA levels increased. Also, when IL-10 levels of hepatitis B 'e' antigen (HBeAg) positive patients were compared with those of HBeAg negative patients, the difference was not statistically significant. CONCLUSION: It is believed that decreasing IL-10 levels by various methods would have significant contributions in disease progression and treatment. Moreover, IL-10 level may be an important marker in HBeAg seroconversion and evaluation of treatment response.

3.
J BUON ; 18(2): 511-5, 2013.
Artículo en Inglés | MEDLINE | ID: mdl-23818370

RESUMEN

PURPOSE: Although influenza and pneumococcal vaccinations for high-risk populations are recommended by current guidelines, vaccination coverage rate (VCR) is still low in patients with malignancies and the family members living with them. METHODS: During the 2011-2012 seasonal influenza (SI), we surveyed 359 patients with solid or hematological malignancies Data were recorded in an especially designed questionnaire after face to face interview. RESULTS: The median patient age was 57 years (range 18-90) and 177 (49.3%) patients were female. Overall vaccination rate was 17% and 4.2% for influenza and pneumococcus, respectively. VCR among family members was 21.2%. The most common causes for not getting vaccinated were lack of knowledge for indication by the patients (33.5%), getting chemotherapy (22.1%), fear of side effects (12.5%), lack of efficacy (12.1%), and not advised by the attending physician (5.9%). CONCLUSION: VCR was very low among patients with cancer and their family members. To eliminate misconceptions and improve vaccination coverage in this population, educational programs for patients and for physicians focusing on safety and efficacy of vaccine are needed.


Asunto(s)
Conocimientos, Actitudes y Práctica en Salud , Vacunas contra la Influenza , Gripe Humana/prevención & control , Neoplasias/inmunología , Percepción , Infecciones Neumocócicas/prevención & control , Vacunas Neumococicas , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Actitud del Personal de Salud , Distribución de Chi-Cuadrado , Femenino , Humanos , Vacunas contra la Influenza/efectos adversos , Gripe Humana/inmunología , Gripe Humana/virología , Masculino , Persona de Mediana Edad , Oportunidad Relativa , Cooperación del Paciente , Educación del Paciente como Asunto , Infecciones Neumocócicas/inmunología , Infecciones Neumocócicas/virología , Vacunas Neumococicas/efectos adversos , Pautas de la Práctica en Medicina , Encuestas y Cuestionarios , Turquía , Vacunación , Adulto Joven
4.
Aging Brain ; 4: 100087, 2023.
Artículo en Inglés | MEDLINE | ID: mdl-37519449

RESUMEN

Background: Though the exact mechanisms regarding brain aging and its relation to neurodegenerative disorders are not precise, oxidative stress, the key regulators of apoptosis and autophagy, such as bcl-2 and beclin 1, seem to be the potential players in the aging of the cerebral cortex and hippocampus. As a type of nicotinamide adenine dinucleotide (NAD+)-dependent deacetylases, sirtuin 2 (SIRT2) has been associated to age-related diseases. However, the exact role of SIRT2 in brain aging is not well studied. The objective of the current study was to study the role of SIRT2 inhibition on brain aging through the neuroprotective mechanisms. Methods: We tested the effects of AGK-2, a SIRT2 inhibitor, on oxidative stress parameters, apoptosis and autophagy regulators including bcl-2, bax, beclin1 in young and old rats. 24 Wistar albino rats (3 months-old and 22 months-old) were divided into four groups; Young-Control (4% DMSO+PBS), Young-AGK-2 (10 µM/bw, ip), Aged-Control, and Aged-AGK-2. Following the 30 days of drug administration period the rats were sacrificed and the cerebral cortex, hippocampus, and cerebellum were isolated. Total antioxidant status (TAS) and total oxidant status (TOS) were measured as oxidative stress parameters in all three brain regions. SIRT2, bcl-2, and bax protein expression levels were measured by western blot and gene expression level of beclin 1, Atg5, and SIRT2 by real-time PCR. Results: The bcl-2, bcl-2/bax ratio, beclin 1, and TAS in the cerebral cortex of the aged group were significantly decreased; however, the TOS, oxidative stress index (OSI), and SIRT2 expression in the cerebral cortex and hippocampus increased. SIRT2 inhibition by AGK-2 reduced TOS and OSI levels in all brain regions and increased bcl-2, bcl-2/bax ratio. In aged animals, AGK-2 also increased the beclin 1 levels in the cortex and hippocampus. Conclusion: Our results indicate that SIRT2 has an essential role in brain aging. The inhibition of SIRT2 by AGK-2 may increase cell survival and decrease aging related processes in the cerebral cortex and hippocampus via decreasing oxidative stress, and increasing bcl-2 and beclin 1 expression.

5.
J Nanosci Nanotechnol ; 12(12): 9058-66, 2012 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-23447958

RESUMEN

In this study, tin/tinoxide/multi oxide/multi walled carbon nano tube (Sn/SnO2/MWCNT) composites were produced by thermal evaporation and then subsequent plasma oxidation. Buckypapers having controlled porosity were prepared by vacuum filtration from functionalized MWCNTs. Pure metallic tin was thermally evaporated on the buckypapers in argon atmosphere with different thicknesses. It was determined that the evaporated pure tin nano crystals were mechanically penetrated into pores of buckypaper to form a nanocomposite. The tin/MWCNT composites were subjected to plasma oxidation process at oxygen/argon gas mixture. Three different plasma oxidation times (30, 45 and 60 minutes) were used to investigate oxidation and physical and microstructural properties. The effect of coating thickness and oxidation time was investigated to understand the effect of process parameters on the Sn and SnO2 phases after plasma oxidation. Quantitative phase analysis was performed in order to determine the relative phase amounts. The structural properties were studied by field-emission gun scanning electron microscopy (FEG-SEM), atomic force microscopy (AFM) and X-ray diffraction (XRD).

6.
J Nanosci Nanotechnol ; 12(12): 9076-87, 2012 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-23447960

RESUMEN

In the present work, Ni/SiC metal matrix composite (MMC) coatings were prepared from a modified Watt's type electrolyte containing nano-SiC particles by direct current (DC) plating method to increase wear resistance of the electro co-deposited Ni. SiC nano particles with average particle size of 0, 1-1 microm were co-deposited with nickel matrix on the steel substrates. For this purpose, several studies were carried out to optimize deposition parameters for specific applications. The depositions were controlled to obtain the specific thickness (between 175-200 microm) and particle volume fraction in the matrix (between 0.03 and 0.10). The hardness of the resultant coatings was measured and found to be 280-571 HV depending on the particle concentration in the electrolyte and therefore, particle volume in the Ni matrix. The tribological tests were performed by a reciprocating ball-on disk apparatus sliding against M50 steel ball (Ø10 mm). All the friction and wear tests were performed under the unlubricated conditions at room temperature and in the ambient air (relative humidity 55-65%). The wear tests were carried out at different sliding velocities of 50 mm/s, 100 mm/s and 150 mm/s by using a constant dead load of 1.0 N. It was detected that increasing sliding speed resulted to increase wear rate but decreasing friction coefficient due to change in wear mechanism. Increasing sliding speed showed formation of tribo induced oxides up to 20 g/l SiC(p) in the electrolyte and wear mechanism was controlled by temperature induced fatigue wear. Further increase in the SiC(p) in the electrolyte caused an abrasive type of the wear.

7.
J Nanosci Nanotechnol ; 12(12): 9169-74, 2012 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-23447973

RESUMEN

In the present study, we report the systematic investigation of the effect of chemical oxidation on the structure of multi-walled carbon nanotubes (MWCNTs) and multi-walled carbon nanotubes buckypaper. The chemical oxidation of multi-walled carbon nanotubes was performed via using three types of chemical solutions: nitric acid, sulfuric acid/nitric acid (3/1) and ammonium hydroxide/hydrogen peroxide (1/1). The surfaces of the multi-walled carbon nanotubes were modified by forming Carboxyl and other functional groups. Flexible multi-walled carbon nanotubes buckypapers were then produced by vacuum filtration techniques from functionalized multi-walled carbon nanotubes. The characteristic properties of multi-walled carbon nanotubes specimens were investigated via Raman, and Fourier Transform Infrared Spectroscopies. The thermal properties and morphology of multi-walled carbon nanotubes were also studied byThermogravimetric Analysis and Field Emission Scanning Electron Microscopy techniques.

8.
West Indian Med J ; 61(2): 139-44, 2012 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-23155958

RESUMEN

AIM: To study the correlation of nasal Staphylococcus aureus carrier status in patients on haemodialysis, infected by hepatitis C virus (HCV), hepatitis B virus (HBV), and their sociodemographic features. SUBJECTS AND METHODS: A survey, including patients' sociodemographic features, was applied to patients by physicians in face to face interviews. Medical records regarding their serologic data were recorded from haemodialysis centres. Nasal swab samples of 2 cm depth from both nostrils of patients were obtained for nasal culture. Samples were inoculated in 5% sheep blood agar and incubated in an incubator at a temperature of 37 degrees C for 24 hours. The results were studied by the same microbiologist. RESULTS: A total of 185 patients were enrolled in the study. According to culture results, 14.1% of patients (n = 26) had methicillin sensitive Staphylococcus aureus (MSSA) and 1.1% (n = 2) had methicillin resistant Staphylococcus aureus (MRSA). Status of viral hepatitis was 3.8% (n = 8), 10.8% (n = 20) for HBV and HCV respectively. Forty per cent (n = 8) of patients with HBV (+) had MSSA carrier status. Statistically significant positive correlation between MSSA and HCV carrier was detected (r = 0.325, p = 0.001) but not between HBV carrier and MSSA (p = 0.255). CONCLUSION: In the present study, significant positivity was detected between MSSA carrier status and HCV in patients on haemodialysis and who have lived together with < or = 2 family members at home. Particularly, statistically significant correlation between HCV(+) and MSSA carrier was observed.


Asunto(s)
Portador Sano/microbiología , Hepatitis B/microbiología , Hepatitis C/microbiología , Cavidad Nasal/microbiología , Diálisis Renal , Staphylococcus aureus/aislamiento & purificación , Anciano , Femenino , Hepatitis B/complicaciones , Hepatitis C/complicaciones , Humanos , Masculino , Staphylococcus aureus Resistente a Meticilina/aislamiento & purificación , Persona de Mediana Edad , Factores Socioeconómicos
9.
J BUON ; 17(3): 457-60, 2012.
Artículo en Inglés | MEDLINE | ID: mdl-23033281

RESUMEN

PURPOSE: The role of genetic factors in the development of cancer is widely accepted. Data on the role of ABO blood group and Rh factor in breast cancer is inconclusive. The aim of this study was to investigate the presence of a possible association between HER2 (+) breast cancer in Turkish women and ABO blood groups and Rh factor. METHODS: In 294 female patients with HER2 (+) breast cancer, ABO blood groups and Rh factor were examined. The relationship of blood groups with age, menopausal status, and family history of cancer, estrogen receptor (ER), progesterone receptor (PR) and HER2 status of these patients was evaluated. Blood groups distribution of 22,821 healthy blood donors was also assessed and compared with the patients' blood groups distribution. RESULTS: The median patient age was 47 years (range 20-80) and 56% of the patients were premenopausal. ER and PR were positive in 50 and 60% of the patients, respectively. Overall, the ABO blood group distribution of the 294 HER2 (+) breast cancer patients was similar to that of the healthy blood donors (p=0.36). Likewise there was no correlation between blood type and ER, PR and menopausal status. Rh (-) patients had more frequent family cancer history and this difference was significant for patients with blood group B Rh (-) and O Rh (-) (p = 0.04). CONCLUSION: In the present study we didn't find any relationship between HER2 status and ABO blood group and Rh factor. However, further studies with larger number of patients are needed to establish the role (if any) of blood groups in patients with breast cancer.


Asunto(s)
Sistema del Grupo Sanguíneo ABO/análisis , Neoplasias de la Mama/sangre , Receptor ErbB-2/análisis , Sistema del Grupo Sanguíneo Rh-Hr/análisis , Adulto , Anciano , Anciano de 80 o más Años , Neoplasias de la Mama/química , Femenino , Humanos , Persona de Mediana Edad
10.
Acta Trop ; 225: 106221, 2022 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-34757042

RESUMEN

We analyzed the peripheral blood lymphocyte subsets of cancer patients infected with intestinal parasites, with an aim to find out the relationship between the levels of different types of lymphocytes with the prognosis of patients. 201 cancer patients aged 18 and over were included. Stool samples of the patients were examined using native-lugol, trichrome, modified trichrome (Weber's Trichrome stain), and modified Ziehl-Neelsen staining methods. Microsporidia and Cryptosporidium parvum were investigated at the genus and species levels using PCR. Lymphocyte subsets were determined by flow cytometry in blood samples. One or more parasite species were detected in 115 (56.7%) patients. The most common parasite species were Microsporidia, Blastocystis and Entamoeba coli, respectively. The frequency of parasites was high in patients with low lymphocyte percentage, CD3+ T cell and CD3+ CD4+ T (Th) cell levels in blood samples studied by flow cytometry. Microsporidia infection was significantly higher in patients with low lymphocyte percentage and Th cell levels. Similarly, C. parvum infection was found to be significantly higher in patients with low T lymphocyte percentage and Th cell level. Finally, Blastocystis infection was significantly higher in patients with low lymphocyte percentage and CD4/CD8 ratio higher than 1. The decrease in lymphocyte percentage, CD3+ T cell and Th cell count, and low CD4/CD8 ratio in cancer patients increase the frequency of intestinal parasitic infections. Based on these results, lymphocyte subsets may help identify cancer patients at high risk of opportunistic parasites. We suggest that opportunistic parasitic infections affecting the clinical course of the disease should be considered by clinicians during the follow-up and treatment of patients.


Asunto(s)
Criptosporidiosis , Parasitosis Intestinales , Subgrupos Linfocitarios , Microsporidiosis/inmunología , Adulto , Criptosporidiosis/inmunología , Cryptosporidium , Heces , Humanos , Parasitosis Intestinales/inmunología , Recuento de Linfocitos , Microsporidios , Prevalencia
11.
Gene Ther ; 17(11): 1333-40, 2010 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-20596057

RESUMEN

We studied the effect of adding chemotherapy or vector targeted chemotherapy to the administration of an Ad-sig-hMUC-1/ecdCD40L adenoviral vector prime-hMUC-1/ecdCD40L protein boost cancer vaccine (designated hMUC-1/ecdCD40L VPPP vaccine), which were administered to test mice 10 days following subcutaneous (s.c.) inoculation of 500,000 Lewis Lung Carcinoma cells, at a time when the average volume of the s.c. tumors was 50 cu mm. The survival of hMUC-1/ecdCD40L VPPP vaccine-treated mice was twice as long as untreated mice. Addition of vector-targeted chemotherapy (AdCMVCDIRESE1A/5FC) to the hMUC-1/ecdCD40L VPPP vaccine 10 days after tumor inoculation significantly (P=0.0062) prolonged the survival of the test mice over administration of the hMUC-1/ecdCD40L VPPP vaccine alone or the control mice (P<0.00001). Interestingly, the combination of the AdCMVCDIRESE1A/5FC vector-targeted chemotherapy to the hMUC-1/ecdCD40L VPPP vaccine decreased the levels of CD44(+)CD24⁻ cells in s.c. deposits of the human MUC-1-positive Lewis Lung Cancer cell line (LL2/LL1hMUC-1) by 20 fold. These results suggest that the addition of vector-targeted chemotherapy to an adenoviral-based cancer vaccine is a strategy that deserves further testing.


Asunto(s)
Adenoviridae/genética , Ligando de CD40/genética , Vacunas contra el Cáncer/uso terapéutico , Carcinoma Pulmonar de Lewis/terapia , Vectores Genéticos/genética , Neoplasias Pulmonares/terapia , Mucina-1/genética , Animales , Vacunas contra el Cáncer/inmunología , Carcinoma Pulmonar de Lewis/tratamiento farmacológico , Carcinoma Pulmonar de Lewis/inmunología , Células Cultivadas , Humanos , Neoplasias Pulmonares/tratamiento farmacológico , Neoplasias Pulmonares/inmunología , Ratones , Ratones Endogámicos C57BL , Linfocitos T Reguladores/inmunología , Células Tumorales Cultivadas
12.
Clin Exp Med ; 9(2): 101-4, 2009 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-19048184

RESUMEN

We aimed to investigate the population of gamma/delta T (gamma/delta T) cells in patients with acute brucellosis. When the bacteria penetrate to the host, the innate immune response aims to prevent the attack by non-activated professional phagocytes. At that moment, macrophages trigger the other cells of the immune system. The cells that can respond immediately are natural killer and gamma/delta T cells. The study included 40 cases of acute brucellosis and 20 healthy volunteers. In this study, it was aimed to compare gamma/delta and alpha/beta (alpha/beta) receptors of the T cells at pre- and post treatment period of patients diagnosed as brucellosis, especially to evaluate the levels of gamma/delta T cells at monitoring of the disease, using flow cytometry. As a result, it was observed that gamma/delta T cells significantly increased in peripheral blood in patients with brucellosis compared with the healthy individuals (13.23 +/- 4.7 and 5.25 +/- 1.4, respectively (p = 0.0001)). gamma/delta T cells were significantly decreased after the brucellosis treatment (p < 0.01). The results of the present study indicate that considerable counts of gamma/delta T cells are involved in acute brucellosis cases. Our findings suggest that gamma/delta TCR bearing cell counts may be used as a supplementary marker for monitoring brucellosis.


Asunto(s)
Brucelosis/inmunología , Receptores de Antígenos de Linfocitos T gamma-delta/fisiología , Enfermedad Aguda , Adolescente , Adulto , Anciano , Brucelosis/etiología , Femenino , Citometría de Flujo , Humanos , Recuento de Linfocitos , Masculino , Persona de Mediana Edad , Receptores de Antígenos de Linfocitos T alfa-beta/fisiología
13.
J BUON ; 14 Suppl 1: S141-6, 2009 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-19785056

RESUMEN

Gene therapy is one of the promising treatment modalities in cancer therapy. The current gene therapy modalities are mainly focused on the introduction of suppressed tumor suppressor genes into cancer cells, modulation of anti-tumoral immune response, and the suicide gene therapy by introducing pro-drug-activating enzyme genes into the tumor cells. Currently, various gene therapy trials are being conducted in cancer patients. However, the early results of these trials conducted so far are not so encouraging. Combination of gene therapy strategies with conventional treatment modalities such as chemotherapy, immunotherapy or radiotherapy has yielded encouraging results in experimental models and early clinical trials.


Asunto(s)
Antineoplásicos/uso terapéutico , Genes Supresores de Tumor/fisiología , Terapia Genética/métodos , Neoplasias/genética , Adenoviridae/genética , Animales , Antineoplásicos/administración & dosificación , Neoplasias Colorrectales/genética , Neoplasias Colorrectales/terapia , Genes Transgénicos Suicidas/genética , Humanos , Ratones , Neoplasias/tratamiento farmacológico , Neoplasias/inmunología , Neoplasias/radioterapia
14.
Ann Clin Lab Sci ; 47(2): 184-190, 2017 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-28442521

RESUMEN

GOALS: To raise awareness of the utility of flow cytometric detection of inflammatory markers in the early diagnosis of neonatal sepsis. PROCEDURES: In accordance with the Töllner scoring system, cases with ≥10 points are accepted as having "clinical sepsis" and cases with 0-4 points as having "no sepsis". The study group consisted of 50 newborns with clinical sepsis as well as a control group of 50 newborns without sepsis. In all cases, blood counts, C-reactive protein (CRP) levels, and procalcitonin (PCT) levels were recorded. Additionally, the "cluster of differentiation" (CD)64, CD11b, and CD62L adhesion molecules and the presence of the human leukocyte antigen HLA-DR on monocyte and neutrophil surfaces were examined by flow cytometry. RESULTS: The levels of acute-phase reactants CRP and PCT were significantly higher in the study group than in the control group (p<0.05). The cell adhesion molecules CD11b and CD64 and the human leukocyte antigen HLA-DR were significantly higher in the study group (p<0.05); CD62L levels were similar to those in the control group (p>0.05). Furthermore, receiver operating characteristic analysis indicated that neutrophil CD11b (nCD11b) is a diagnostic marker for neonatal sepsis (area under the curve [AUC]: 0.72, 95% confidence interval [CI]: 0.62-0.82, p<0.001). The sensitivity, specificity, and positive predictive value (PPV) for nCD11b were 72%, 68%, and 58.4%, respectively. Similarly, monocyte CD11b (mCD11b) positivity was found to be diagnostic (AUC 0.77, 95% CI: 0.68-0.87, p<0.001). The sensitivity and specificity for mCD11b were 72% and 68%, respectively. In addition, the sensitivity, specificity, and PPV for nHLA-DR were 62%, 60%, and 60.8%, respectively. CONCLUSION: In addition to acute-phase proteins, cell surface antigens such as CD11b, CD64, and HLA-DR should be used in routine investigations for the early diagnosis of neonatal sepsis. Such usage in combination with acute-phase reactants may enhance diagnostic accuracy.


Asunto(s)
Diagnóstico Precoz , Citometría de Flujo/métodos , Sepsis Neonatal/diagnóstico , Proteínas de Fase Aguda/metabolismo , Antígenos de Superficie/metabolismo , Demografía , Femenino , Humanos , Recién Nacido , Masculino , Monocitos/patología , Sepsis Neonatal/sangre , Sepsis Neonatal/microbiología , Neutrófilos/patología
15.
Ann R Coll Surg Engl ; 99(3): 233-236, 2017 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-27791426

RESUMEN

INTRODUCTION Non-diagnostic results of fine needle aspiration biopsy (FNAB) remain an important limitation of this technqiue. The aim of our study was to evaluate the results of core needle biopsy (CNB) of thyroid nodules and its effectiveness in non-diagnostic FNAB cases. METHODS CNBs were performed in 1,000 patients (154 male, 846 female; mean age: 50.2 years, range: 18-86 years) with a spring loaded 20G needle. Of these, 143 had initially had FNABs that were insufficient for evaluation. The CNB reports were reviewed. Patients with suspicious or malignant CNB results underwent total thyroidectomy. RESULTS When considering all 1,000 CNBs, the non-diagnostic rate was 1.5% (15/1,000). However, when the first 100 cases were eliminated as a learning curve, this reduced to 0.9% (8/900). Of the 143 cases with initial FNABs that were non-diagnostic, 0.7% (1/143) were also non-diagnostic on CNB. Twelve patients underwent surgery because of malignant CNB reports and all of these cases were confirmed as malignant by the postoperative pathology specimen results (100% accuracy). There were no major complications although three self-limiting minor complications were observed. CONCLUSIONS CNB is a safe and accurate method. It is more diagnostic than FNAB for nodular thyroid disease.


Asunto(s)
Biopsia con Aguja Gruesa , Carcinoma Neuroendocrino/patología , Carcinoma/patología , Carcinoma Anaplásico de Tiroides/patología , Neoplasias de la Tiroides/patología , Nódulo Tiroideo/patología , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Carcinoma/diagnóstico , Carcinoma/cirugía , Carcinoma Neuroendocrino/diagnóstico , Carcinoma Neuroendocrino/cirugía , Carcinoma Papilar , Femenino , Humanos , Biopsia Guiada por Imagen , Masculino , Persona de Mediana Edad , Cáncer Papilar Tiroideo , Carcinoma Anaplásico de Tiroides/diagnóstico , Carcinoma Anaplásico de Tiroides/cirugía , Neoplasias de la Tiroides/diagnóstico , Neoplasias de la Tiroides/cirugía , Nódulo Tiroideo/diagnóstico , Nódulo Tiroideo/cirugía , Tiroidectomía , Ultrasonografía , Adulto Joven
16.
Cancer Gene Ther ; 13(4): 346-56, 2006 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-16179927

RESUMEN

We constructed a conditionally replication-competent adenoviral vector Ad.Lp-CD-IRES-E1A(control) in which the expression of both the prodrug-activating cytosine deaminase gene and the viral replication E1A gene were driven by the L-plastin tumor-specific promoter. In order to overcome the low infectivity of the adenoviral vectors for breast cancer cells, and to increase the safety and efficacy for cancer gene therapy, this vector was further modified on a transductional level by simultaneously ablating the native tropism of the vector to the primary CAR receptor and inserting a RGD-4C peptide into the HI loop of the fiber, which allows the vector to use the alphavbeta3 and alphavbeta5 receptors as alternative receptors. The resulting vector was named Ad.Lp-CD-IRES-E1A(MRGD). The transduction efficiency of the vector for breast cancer cell lines which have low expression level of CAR was increased both in vitro and in vivo. The Ad.Lp-CD-IRES-E1A(MRGD) vector produces a higher vector particle yield and a greater cytotoxic effect in tumor cells which have a low expression level of CAR, than did the Ad.Lp-CD-IRES-E1A(control) vector. Intratumoral injection of the Ad.Lp-CD-IRES-E1A(MRGD) vector following the intraperitoneal injection of 5FC into xenotransplanted human breast cancer cell lines which have low expression level of CAR led to greater degree of tumor regression in vivo than did the intratumoral injection of control adenoviral vectors not so modified.


Asunto(s)
Adenoviridae/fisiología , Citosina Desaminasa/genética , Terapia Genética , Vectores Genéticos , Neoplasias Mamarias Experimentales/terapia , Proteínas E1A de Adenovirus/genética , Proteínas E1A de Adenovirus/metabolismo , Animales , Antimetabolitos Antineoplásicos/metabolismo , Antimetabolitos Antineoplásicos/farmacología , Línea Celular Tumoral , Proliferación Celular/efectos de los fármacos , Citosina Desaminasa/biosíntesis , Femenino , Flucitosina/metabolismo , Flucitosina/farmacología , Humanos , Integrina alfaVbeta3/metabolismo , Integrinas/metabolismo , Neoplasias Mamarias Experimentales/metabolismo , Neoplasias Mamarias Experimentales/patología , Glicoproteínas de Membrana , Proteínas de Microfilamentos , Oligopéptidos/genética , Oligopéptidos/metabolismo , Fosfoproteínas/genética , Fosfoproteínas/metabolismo , Regiones Promotoras Genéticas , Receptores de Vitronectina/metabolismo , Transducción Genética
17.
Int J Biol Markers ; 21(4): 206-10, 2006.
Artículo en Inglés | MEDLINE | ID: mdl-17177157

RESUMEN

BACKGROUND: Venous thromboembolism (VT) increases mortality and morbidity in cancer patients. The primary aim of this study was to evaluate the effect of VT on the survival of cancer patients and its relationship with serum vascular endothelial growth factor (VEGF) and plasma factor VIII levels. PATIENTS AND METHODS: Eighty-two patients with locally advanced or metastatic cancer were included in this study between September 2001 and March 2004, and 31 of them had VT. Fifty-one matched-paired cancer patients without VT were prospectively selected as a control group in the same period. Criteria for the selection of control group patients were having the same malignancy, stage, metastatic site, performance status and age (+/-5 years) as patients in the VT group. RESULTS: Plasma factor VIII and serum D-dimer levels in the VT group were significantly higher than those in the control group (p=0.030 and p=0.016, respectively). However, mean serum VEGF levels were similar in both groups (p=0.199). In the VT group, the median survival of patients who had higher serum VEGF levels (>150 pg/mL) was significantly shorter than that of patients in the same group with lower serum VEGF levels (p=0.005). The median survival of the VT group was 14 months, whereas it was 25 months in the control group (p=0.199). CONCLUSION: There was a worse prognostic trend for cancer patients with VT. Nevertheless, the difference in survival was not statistically significant between the groups. Plasma factor VIII and serum D-dimer levels might have prognostic value in cancer patients with VT. Cancer patients with VT and higher serum VEGF levels had a significantly poorer prognosis.


Asunto(s)
Factor VIII/análisis , Neoplasias/mortalidad , Factor A de Crecimiento Endotelial Vascular/sangre , Trombosis de la Vena/sangre , Adulto , Anciano , Productos de Degradación de Fibrina-Fibrinógeno/análisis , Humanos , Persona de Mediana Edad , Neoplasias/sangre , Estudios Prospectivos
18.
FEMS Immunol Med Microbiol ; 45(2): 253-8, 2005 Aug 01.
Artículo en Inglés | MEDLINE | ID: mdl-15949930

RESUMEN

The aim of the study was to evaluate intracellular interferon-gamma (IFN-gamma), and interleukin-4 (IL-4) levels in pre- and post-treatment periods of brucellosis patients and to determine the relationship between these parameters and patients' clinical findings. Twenty-five patients diagnosed as brucellosis and 11 aged-matched healthy volunteers were included in the study. CD3+CD4+ T lymphocytes levels were significantly lower in patients with brucellosis as compared to the control group. CD3+CD8+ T lymphocytes and CD3+IFN-gamma+ levels were increased in brucellosis patients compared with the control group. CD4+IFN-gamma+ and CD4+IL-4+ levels were no different between patients and healthy individuals. CD3+IL-4+ levels decreased in patients compared with healthy controls. Pre-treatment CD3+IFN-gamma+ levels dramatically increased in patients responsive to management compared with the unresponsive ones. In responsive cases, CD3+IFN-gamma+ levels decreased statistically after the treatment while in unresponsive cases no meaningful change was observed with respect to treatment. Adding IFN-gamma to the treatment for improving the depleted levels of IFN-gamma can be beneficial in patients with brucellosis who shows a tendency to chronicity or patients who do not respond to the treatment.


Asunto(s)
Brucelosis/inmunología , Citocinas/biosíntesis , Células TH1/inmunología , Células Th2/inmunología , Adolescente , Adulto , Anciano , Antibacterianos/uso terapéutico , Brucelosis/tratamiento farmacológico , Estudios de Casos y Controles , Citocinas/análisis , Femenino , Citometría de Flujo , Humanos , Interferón gamma/análisis , Interferón gamma/biosíntesis , Interferón gamma/uso terapéutico , Interleucina-4/análisis , Interleucina-4/biosíntesis , Masculino , Persona de Mediana Edad , Proteínas Recombinantes
19.
Basic Clin Pharmacol Toxicol ; 97(5): 325-32, 2005 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-16236146

RESUMEN

We have studied the effects of three antioxidants and amrinone, an inotropic agent, against vancomycin-induced nephrotoxicity in rats by investigating renal function and morphology. Thirty adult female Sprague Dawley rats (168-234 g) were divided into six groups. A saline-treated group served as control. The other five groups were treated for 7 days with vancomycin alone or in combination with alpha-lipoic acid, Ginkgo biloba extract 761, melatonin or amrinone. On day 8, all the rats were sacrificed by decapitation, kidney tissues were excised immediately and blood and kidney samples were collected. Blood urea and creatinine, kidney tissue malondialdehyde levels, and kidney superoxide dismutase and glutathione (GSH) peroxidase activities were measured. The kidneys were also examined for histological changes. Vancomycin administration led to increased urea, creatinine and malondialdehyde levels and decreased superoxide dismutase and GSH peroxidase activities. Co-administration of alpha-lipoic acid, Ginkgo biloba extract, melatonin or amrinone with vancomycin prevented the increases in the urea, creatinine and melondialdehyde levels and also resulted in higher superoxide dismutase and GSH peroxidase activities. The antioxidants and AMR improved the renal pathology compared to rats treated with vancomycin alone (P<0.05). These results indicate that the three antioxidants and amrinone have potential protective effects against vancomycin-induced nephrotoxicity, which might in part be due to inhibition of free oxygen radical production. Amrinone was the most effective drug as judged on the basis of the pathological findings.


Asunto(s)
Amrinona/farmacología , Antibacterianos/antagonistas & inhibidores , Antibacterianos/toxicidad , Antioxidantes/farmacología , Enfermedades Renales/inducido químicamente , Enfermedades Renales/prevención & control , Inhibidores de Fosfodiesterasa/farmacología , Vancomicina/antagonistas & inhibidores , Vancomicina/toxicidad , Animales , Femenino , Radicales Libres/metabolismo , Ginkgo biloba , Glutatión Peroxidasa/metabolismo , Corteza Renal/metabolismo , Corteza Renal/patología , Enfermedades Renales/patología , Pruebas de Función Renal , Malondialdehído/metabolismo , Melatonina/farmacología , Ratas , Ratas Sprague-Dawley , Superóxido Dismutasa/metabolismo , Sustancias Reactivas al Ácido Tiobarbitúrico/metabolismo , Ácido Tióctico/farmacología
20.
Exp Oncol ; 37(1): 53-7, 2015 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-25804233

RESUMEN

AIM: Identification of patient with increased risk of cardiotoxicity would allow not only prevention and early diagnosis of chemotherapy related cardiotoxicity but also administration of optimal dose and duration of chemotherapy. MATERIALS AND METHODS: Fifty-two women with HER2(+) breast cancer treated with trastuzumab were included in this study. Patients were prospectively followed with routine cardiac evaluation. Before and after administration of trastuzumab blood samples for NT-proBNP were also taken. RESULTS: The median age was 48.5 year (range: 26-74). Hypertension and obesity were two most common co-morbidities. The median duration application of trastuzumab was 52 weeks. During median 14.5 (3-33) months follow-up cardiac adverse events occurred in 5 (9.6%) patients and 2 out of 5 was grade III-IV heart failure. Both patients had preserved left ventricular ejection fraction and no symptom of heart failure before trastuzumab but older than 65 years old and had diabetes mellitus and obesity. High level of NT-proBNP (> 300 ng/ml) was observed in both patients and heart failure recovery was not observed. There was statistically significant difference regarding body mass index (p = 0.004) and diabetes mellitus (p = 0.002) between patients with and without cardiotoxicity. CONCLUSION: Although, cardiac biomarkers still cannot replace routine cardiac monitoring, natriuretic peptides may provide additional tool for detection of patients with high risk of cardiotoxicity and early detection of cardiotoxicity.


Asunto(s)
Anticuerpos Monoclonales Humanizados/toxicidad , Antineoplásicos/toxicidad , Neoplasias de la Mama/complicaciones , Neoplasias de la Mama/tratamiento farmacológico , Cardiotoxicidad/sangre , Cardiotoxicidad/complicaciones , Péptido Natriurético Encefálico/sangre , Fragmentos de Péptidos/sangre , Adulto , Anciano , Anticuerpos Monoclonales Humanizados/uso terapéutico , Antineoplásicos/uso terapéutico , Biomarcadores/sangre , Mama/efectos de los fármacos , Neoplasias de la Mama/sangre , Cardiotoxicidad/diagnóstico , Femenino , Estudios de Seguimiento , Corazón/efectos de los fármacos , Humanos , Persona de Mediana Edad , Trastuzumab
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA