Qualitative and Quantitative Assessment of Alveolar Bone Destruction in Adult Rats Using CBCT.

Cone-beam computed tomography (CBCT) has strong potential to be utilized in various aspects of veterinary dentistry. Using ex vivo rat maxillary bone and teeth, the purpose of this study was to compare gray value, surface area, and volumetric measurements of teeth with and without experimental periodontitis by CBCT. Periodontitis was induced in 36 molar teeth, while 36 teeth with a healthy periodontium served as control. Images of each specimen along with teeth were obtained using CBCT. The following measurements for each tooth with periodontitis (n = 36) were recorded: gray value measurement, width, height, depth, surface area, and volume of the alveolar bone loss. For the control group (n = 36), gray value measurement, surface area, and volume of the alveolar bone were recorded. All measurements were repeated after 3 weeks. As the gold standard, the rat maxillas were decalcified and paraffin-embedded for further immunocytochemical study. One-way analysis of variance (ANOVA) was used. Significance level was set at P < .05. Correlation values for gray value, width, height, depth, surface area, and volume measurements were 0.983, 0.966, 0.962, 0.880, 0.998, and 0.999, respectively, for the first and second measurements. One way ANOVA showed statistically significant differences between teeth with and without alveolar bone destruction conducted for gray value, surface area, and volume measurements (P = .000). Mean gray value, surface area, and volume measurements decreased 56.46%, 81.89%, and 78.56%, respectively, for teeth with alveolar bone destruction in comparison to healthy teeth. Cone-beam computed tomography provided useful qualitative and quantitative information regarding induced periodontitis in the rat maxilla.

Effect of voxel size on accuracy of cone beam computed tomography-aided assessment of periodontal furcation involvement.

OBJECTIVE: To assess the accuracy of cone beam computed tomography (CBCT) images obtained at different voxel sizes in detecting ligature-induced furcation involvement. STUDY DESIGN: Furcation lesions were prepared in 36 molar teeth in rats, and 24 intact teeth served as controls. CBCT Kodak 9000 images at three different voxel sizes were acquired: 0.076 mm, 0.100 mm, and 0.200 mm. Four observers assessed 3 sets of images using a 5-point evaluation scale. κ-Coefficients were calculated for intra- and interobserver agreement. Receiver operating characteristic analysis measured the true positive rate (TPR), true negative rate (TNR), and area under the curve (AUC). Accuracy values were compared by using Mood's Median Chi-Square (α<0.05). RESULTS: Intra- and interobserver agreement ranged from 0.600 to 0.999 and from 0.366 to 0.573, respectively. Highest median AUC and true positive rate (TPR) values were obtained for voxel size of 0.076 mm. The highest median TNR values were obtained for the voxel size 0.1 mm. There were no significant differences (P≥.05) among the median AUC, TPR, or TNR values for the 3 different CBCT voxel sizes. CONCLUSIONS: Given the limitations of this study, all voxel sizes performed similarly in the detection of furcation involvement.