Morphological identification of Amphitetranychus species (Acari: Tetranychidae) with crossbreeding, esterase zymograms and DNA barcode data.

The genus Amphitetranychus Oudemans (Tetranychidae) consists of only three species, A. quercivorus (Ehara & Gotoh), A. savenkoae (Reck) and A. viennensis (Zacher). The original description of A. savenkoae was extremely simple and had no drawing of the aedeagus; however, a subsequent study described only the aedeagus. The present study investigated all three species in detail using a combination of morphological traits, crossbreeding experiments, esterase zymograms and the mitochondrial cytochrome c oxidase subunit I (COI) gene. Morphological differences in the peritremes and male aedeagi were observed among the three species. Complete reproductive isolation was confirmed in the reciprocal crosses between the morphologically similar A. savenkoae and A. quercivorus (no female offspring were produced). Esterase zymograms differed interspecifically, but not intraspecifically (among individuals in a given species). All three species formed clearly separate clades with 100% bootstrap values in the COI tree, and A. savenkoae was more closely related to A. quercivorus than to A. viennensis, which corresponded to the morphological similarity of their aedeagi and setal counts on tarsi IV. A key to Amphitetranychus species is provided.

Borrelia and Other Zoonotic Pathogens in Ixodes ricinus and Dermacentor reticulatus Ticks Collected from the Chernobyl Exclusion Zone on the 30th Anniversary of the Nuclear Disaster.

Background: The 26th of April 2016 marked 30 years since the Chernobyl accident has occurred in Ukraine. As a result, the uninhabited Chernobyl region has been directly exposed to ionizing radiation for >30 years. Most work has focused on identifying associations between levels of radiation and the abundance, distribution, and mutation rates of plants and animals. Much less, however, is known about microbial communities in this affected region. To date, there are no reports on the prevalence of any tick-borne pathogens in Ixodes ricinus ticks from the Chernobyl exclusion zone (CEZ). The objective of our study was to examine the abundance of I. ricinus and Dermacentor reticulatus ticks in the CEZ and to investigate the prevalence of Borrelia burgdorferi sensu lato (s.l.) and other zoonotic agents in these ixodid ticks. Methods: A total of 260 questing I. ricinus and 100 D. reticulatus adult ticks were individually polymerase chain reaction analyzed for the presence of Anaplasma phagocytophilum, Babesia spp., Bartonella spp., Borrelia burgdorferi s.l., Francisella tularensis, and/or Rickettsia spp. Results: The respective infections rates were identified and compared with those of ixodid ticks that were concurrently collected from Kyiv. The significant differences between the infection rates of the CEZ and Kyiv ticks were observed for Rickettsia raoultii in D. reticulatus ticks (53.0% vs. 35.7%, respectively; p < 0.05) and Bartonella spp. (8.1% vs. 2.7%; P < 0.05) in I. ricinus ticks. Conclusions: Although the current data clearly demonstrated that the prevalence of some zoonotic pathogens were significantly higher in the ixodid ticks from the CEZ, a more comprehensive systematic approach is required to examine the causal effect of long-term ionizing radiation on adaptive changes of tick-borne pathogens.

Emergence of tick-borne pathogens (Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum, Ricketsia raoultii and Babesia microti) in the Kyiv urban parks, Ukraine.

To date, only limited data about the presence of ticks and circulation of tick-borne pathogens in urban parks of Kyiv in northern Ukraine are available. In total, 767 ticks (696 Ixodes ricinus and 69 Dermacentor reticulatus) collected in seven urban parks and one suburban oak wood park in Kyiv were individually analyzed by the PCR assays. Tick-borne pathogens, namely spirochetes from Borrelia burgdorferi sensu lato complex, Anaplasma phagocytophilum, and Babesia microti, were detected in 11.1% of tested I. ricinus ticks. In total, 4% of I. ricinus ticks tested positive for the presence of B. burdorferi s.l. (Borrelia afzelii and Borrelia garinii), 5.2% for A. phagocytophilum, and Ba. microti was confirmed in 1.9% of examined ticks. Mixed infections were recorded in four DNA samples, representing the prevalence of 0.6%. One female and two I. ricinus nymphs were simultaneously infected with B. afzelii and A. phagocytophilum, and one female carried B. afzelii and Ba. microti. In addition, 10.1% of D. reticulatus ticks tested positive for Rickettsia raoultii. Identification of infectious agents and their diversity, assessment of the relative epidemiological importance and determination of the prevalence in questing ticks from central parts of the cities are crucial steps towards the tick-borne diseases surveillance in urban environment.

The infection of questing Dermacentor reticulatus ticks with Babesia canis and Anaplasma phagocytophilum in the Chernobyl exclusion zone.

Tick occurrence was studied in the Chernobyl exclusion zone (CEZ) during the August-October 2009-2012. Dermacentor reticulatus ticks were collected using the flagging method and then screened for infection with Anaplasma phagocytophilum and Babesia canis by a PCR method incorporating specific primers and sequence analysis. The prevalence of infection with B. canis canis and A. phagocytophilum was found to be 3.41% and 25.36%, respectively. The results present the first evidence of B. canis canis and A. phagocytophilum in questing D. reticulatus ticks from the Chernobyl exclusion zone. They also reveal the presence of tick-borne disease foci in areas with no human activity, and confirm that they can be maintained in areas after a nuclear disaster with radioactive contamination.