Secretion and Reversible Assembly of Extracellular-like Matrix by Enzyme-Active Colloidosome-Based Protocells.

The secretion and reversible assembly of an extracellular-like matrix by enzyme-active inorganic protocells (colloidosomes) is described. Addition of N-fluorenyl-methoxycarbonyl-tyrosine-(O)-phosphate to an aqueous suspension of alkaline phosphatase-containing colloidosomes results in molecular uptake and dephosphorylation to produce a time-dependent sequence of supramolecular hydrogel motifs (outer membrane wall, cytoskeletal-like interior and extra-protocellular matrix) that are integrated and remodelled within the microcapsule architecture and surrounding environment. Heat-induced disassembly of the extra-protocellular matrix followed by cooling produces colloidosomes with a densely packed hydrogel interior. These procedures are exploited for the fabrication of nested colloidosomes with spatially delineated regions of hydrogelation.

The synthesis of Tween80/calcium phosphate nanocomposite bioactive gelatine-based gels as a proof of concept for tooth sensitivity home-treatment.

Dentine hypersensitivity interferes with the lifestyle owing to pain arising from exposed dentine surfaces in response to stimuli. One common way to treat this problem is to occlude the exposed tubules. In this work, we have proposed a home-based treatment gel for tooth sensitivity. The gel was prepared using the emulsion method and contained Tween80/calcium phosphate nanocomposite that could occlude the tubules after 10 h of application. In preparation, Tween80 was used as a surfactant, and oleic acid was used as an oil phase to form a water-in-oil nanoreactor for calcium phosphate synthesis. Finally, different concentrations of gelatine were used to transform the emulsion into a stable gel. The nanoparticles had a uniform spherical shape and a diameter of approximately 300 nm. The nanocomposite gel containing the lowest amount of gelatine (Gel-T80-5%GE) exhibited the best liquid-like property and the highest occlusion rate of 95%.

Fabrication of calcium phosphate composite polymer/SLS-stabilized emulsion-based bioactive gels and their application for dentine tubule occlusion.

OBJECTIVES: Calcium phosphate/SLS/P123 composite bioactive gels were prepared to achieve dentine tubule occlusion. METHODS: Gels containing calcium phosphate particles were prepared in a water-in-oil microemulsion system with a mixture of triblock copolymer pluronic (P123) as a co-surfactant and sodium lauryl sulfate (SLS) as a surfactant in cyclohexane. Subsequently, calcium chloride dihydrate and sodium hydrogen phosphate aqueous solutions were added in a water phase. Finally, slow evaporation of the oil phase at room temperature was performed to produce a hybrid gel. The obtained gels were investigated for their toxicity by the sulforhodamine B (SRB) assay and applied on human dentine specimens to examine their ability to occlude dentine tubules. RESULTS: The size and morphology of the calcium phosphate particles embedded in the gel depended on the concentration of P123 and SLS, which were used as a template for mineral precipitation. The prepared calcium phosphate particles (200-500 nm in diameter) with the maximum polymer and surfactant content exhibited spherical shapes. Further, on reducing their content twice and tenfold yields micro-particles with flower-like shapes. These bioactive gels were able to occlude into dentine tubules after 3 days of application with a plugging rate of 79.22% when using the smallest particles. In addition, calcium phosphate nanorods were transformed into dentine tubules with a maximum depth of 6 µm on increasing the amount of gel. CONCLUSIONS: The bioactive gels were effectively used as bioactive fillers to occlude exposed human dentine tubules.