Biology of Nicotiana glutinosa L., a newly recorded species from an archaeological excavation site in Egypt.

BACKGROUND: During a field survey of urban flora in Alexandria city in 2019-2022, an interesting species belonging to the Solanaceae was collected from a newly archaeological excavation site and identified as Nicotiana glutinosa L. Many visits were made to the herbaria of Egypt to confirm the species records, but no single record was found. Reviewing the available literature revealed that this tropical American taxon was never recorded in the flora of Egypt. AIMS: The present study was focused on N. glutinosa growth structure and plant macro- and micromorphology. METHODS: Ten sampling sites were covered for N. glutinosa size structure. Plant samples were examined for stem anatomy, leaf, seed, and pollen morphology. RESULTS: The species size structure reveals that the individual size index ranges from 1.33 to 150 cm, while its density ranges from 4 to 273 individuals /100 m-2. N. glutinosa has successfully established itself in one of the archaeological sites in Egypt, showing a "healthy" population with a high degree of size inequality, characterized by a relative majority of the juvenile individuals. Voucher specimens were deposited in the Herbarium of Alexandria University (ALEX) Faculty of Science, another specimen is processed to make herbarium specimens at the Herbarium of the Botanic Garden (Heneidy et al. collection, deposition number. 5502). CONCLUSIONS: From our observations, N. glutinosa seems to have invasive potential, as it shows characteristics shared by most invasive species that are thought to help in their successful establishment in new habitats. This article emphasizes the importance of monitoring and regularly reporting the threats of alien invasive species to avoid any possible negative impacts on indigenous biodiversity in the future.

Temporal Potential of Phragmites australis as a Phytoremediator to Remove Ni and Pb from Water and Sediment in Lake Burullus, Egypt.

In the current work, we investigated the concentration of Ni and Pb in different organs of Phragmites australis to evaluate its potential application as a phytoremediator to remove these two metals from contaminated water and sediment in Lake Burullus (a Ramsar site in Egypt). Above- and below-ground biomass of P. australis, water and sediment were sampled monthly for 1 year at six sites of Lake Burullus (three sites represent each of the northern and southern parts of the lake) using six randomly distributed quadrats (each of 0.5 × 0.5 m) at each sampling site. Significant variation was detected for Ni and Pb concentrations in the sediments and waters between the northern and southern sites of the lake. The biomass of P. australis in the southern sites was greater than that in the northern sites; in addition, the above-ground biomass was higher than the below-ground biomass. The above-ground organs accumulated higher concentrations of Ni and Pb than the below-ground organs. The Ni and Pb standing stocks data indicated that the organs of P. australis extracted higher amounts of Ni and Pb per its area from the southern rather than the northern sites. In the current study, the Ni and Pb above-ground standing stocks increased from the early growing season (February) and reached its peak during August and then decreased. The highest monthly Ni and Pb standing stock (18.2 and 18.4 g m- 2, respectively) was recorded in the above-ground organs of plants in the southern sites in August. The bioaccumulation factor of Ni was 157.6 and 153.4 in the northern and southern sites, respectively, whereas that of Pb was 175.3 and 158.3. The translocation factor of Ni and Pb from the below- to above-ground organs was generally > 1. Thus, this reed species is a potential candidate for Ni and Pb phytoextraction. Based on our results, P. australis could be used for the extraction of Ni and Pb to reduce the pollution in Lake Burullus, if the above-ground biomass is harvested at its maximum value in August, as was the case regarding the maximum standing stock of Ni and Pb.

Phytostabilization as a phytoremediation strategy for mitigating water pollutants by the floating macrophyte Ludwigia stolonifera (Guill. & Perr.) P.H. Raven.

The present study evaluated the phytoremediation potential of the floating macrophyte Ludwigia stolonifera for removing trace metals from contaminated water bodies. Forty quadrats, distributed equally in eight sites (six polluted two unpolluted sites) were selected seasonally for water, sediment and plant investigations. The leaf area, fresh and dry biomass, chlorophyll b and carotenoids contents of L. stolonifera were significantly reduced in polluted sites. L. stolonifera plants accumulated concentrations of the investigated trace metals in their roots higher than the shoots. The roots contributed to the highest concentrations of Al and Cu during spring; Fe, Mn and Ni during summer; Cd and Zn during autumn; and Cr and Pb during winter. Compared to the unpolluted sites, the below- and above-ground parts from the polluted sites accumulated higher concentrations of most investigated trace metals, except Fe. The below-ground parts of L. stolonifera had high seasonal potential for seasonal accumulation of Cd, Cu, Ni, Zn and Pb with a bioaccumulation factor that exceeded 1, the translocation factor of the investigated metals was <1. Therefore, the study species is suitable for metals phytostabilization and thus can be considered a potential phytoremediator of these metals.

Pattern of Urban Flora in Intra-City Railway Habitats (Alexandria, Egypt): A Conservation Perspective.

Intra-city railway areas are deemed large greenspaces and are believed to be key in enhancing the diversity and dynamics of urban flora. In the current study, the floristic composition and diversity along intra-city railway and tram tracks in Alexandria were surveyed. The floristic composition of the plant communities in relation to environmental factors, ecological indicators, and level of human impact was analyzed using multivariate analysis (two-way indicator species analysis (TWINSPAN) for classification and detrended correspondence analysis (DECORANA) for ordination. The multivariate ordination techniques (CCA) revealed differences in the environmental factors and climatic factors influencing the floristic composition of the railway and tram track habitats. Tram tracks suffered higher human impact while maintaining higher vitality and cover compared to train tracks. Species recorded were mainly therophytes, followed by phanerophytes and hemicryptophytes dominated by native species; however, invasive species' contribution was higher compared to surrounding regions. The number of invasive species was greater in railway areas compared to tram track areas (19 and 15, respectively). The occurrence of two endemic species (Sinapis allionii and Sonchus macrocarpus) with limited national distribution highlights the importance of these habitats as valuable refuge areas for rare and endangered species worthy of conservation action.

Ecological Distribution and Genetic Variations of Some Aloe Species in Taif, KSA.

BACKGROUND AND OBJECTIVES: Aloe is a medicinally and economically important genus. Many Aloes seem an endangered species because of over-collection, destruction of plants and destroyed of natural habitats. The objectives of current study was to survey, collect and identification of some Aloe species and to analyze genetic variations between the collected Aloe species. MATERIALS AND METHODS: Four Aloe species (A. armatissima, A. edentata, A. parvicoma and A. pseudorubroviolacea) and Agave americana (Asperagaceae) were used as plant materials for ecological and genetic studies. In RAPD and ISSR analysis 23 and 16 primers, respectively were screened. RESULTS: Ecological study showed that the 4 species are endemic: 2 are endangered (A. edentata and A. parvicoma) and the others are not-endangered (A. armatissima and A. pseudorubroviolacea), while A. americana was introduced as ornamental species. Concerning RAPD, a total of 134 reproducible bands of them 131 bands are polymorphic ~ 97.65% polymorphism were produced, which ranged from 9 bands (primer OPC-04) to 18 (primer OPA-03) bands, with an average 13.4 bands/ primer, ranging from ~300-2500 bp. According to ISSR, 113 reproducible bands were totally yielded with an average 12.6 bands/primer, from ~180-1500 bp, of which 107 poly-morphic bands number (PBN) ~94.96% polymorphism ranged from 10 bands (primer UBC-818 and primer UBC-819) to 14 (primer UBC-814) with an average of 11.9 PB/primer. CONCLUSION: The results revealed high genetic variations between 4 bands Aloe species and A. americana species, which will be in concern for improvement, breeding and conservation programs.