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1.
J Cell Sci ; 133(9)2020 05 14.
Artículo en Inglés | MEDLINE | ID: mdl-32409568

RESUMEN

Salmonella enterica is an intracellular bacterial pathogen. The formation of its replication niche, which is composed of a vacuole associated with a network of membrane tubules, depends on the secretion of a set of bacterial effector proteins whose activities deeply modify the functions of the eukaryotic host cell. By recruiting and regulating the activity of the kinesin-1 molecular motor, Salmonella effectors PipB2 and SifA play an essential role in the formation of the bacterial compartments. In particular, they allow the formation of tubules from the vacuole and their extension along the microtubule cytoskeleton, and thus promote membrane exchanges and nutrient supply. We have developed in vitro and in cellulo assays to better understand the specific role played by these two effectors in the recruitment and regulation of kinesin-1. Our results reveal a specific interaction between the two effectors and indicate that, contrary to what studies on infected cells suggested, interaction with PipB2 is sufficient to relieve the autoinhibition of kinesin-1. Finally, they suggest the involvement of other Salmonella effectors in the control of the activity of this molecular motor.This article has an associated First Person interview with the first author of the paper.


Asunto(s)
Salmonella enterica , Proteínas Bacterianas , Células HeLa , Humanos , Cinesinas/genética , Salmonella , Vacuolas
2.
Microb Pathog ; 51(3): 203-8, 2011 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-21497651

RESUMEN

Nitric oxide (NO) produced by inducible nitric oxide synthase (iNOS) is a radical effector molecule of the innate immune system that can directly inhibit pathogen replication. In order to study subsequent iNOS kidney expression in experimental leptospirosis, Golden Syrian hamsters and C3H/HeJ mice were infected intraperitoneally with 10(2) or 10(7) virulent Leptospira interrogans serovar Copenhageni (LIC) strain Fiocruz L1-130. Results showed increased levels of iNOS mRNA and protein in kidneys of infected animals when compared to that in mock-infected animals. To get a deeper insight into the role of iNOS in experimental leptospirosis, both subject species were treated or not treated with 4-aminopyridine (4-AP, 0.3mg/kg), an iNOS inhibitor. Treatment of infected hamsters with 4-AP accelerated the mortality rate to 100% by one day and increased the mortality rate from 20 to 60% in mice at 14 days post-infection. In kidney tissues, 4-AP treatment increased the bacterial burden, as demonstrated through leptospiral DNA quantification by real-time PCR, and aggravated tubulointerstitial nephritis. In addition, iNOS inhibition reduced the specific humoral response against LIC when compared to that in untreated infected animals. According to these results, iNOS expression and the resulting NO have an important role in leptospirosis.


Asunto(s)
Leptospira interrogans/inmunología , Leptospirosis/inmunología , Óxido Nítrico Sintasa de Tipo II/inmunología , Animales , Carga Bacteriana , Cricetinae , Modelos Animales de Enfermedad , Expresión Génica , Perfilación de la Expresión Génica , Riñón/inmunología , Riñón/microbiología , Riñón/patología , Mesocricetus , Ratones , Ratones Endogámicos C3H , Nefritis/inmunología , Nefritis/microbiología , Nefritis/patología , Enfermedades de los Roedores/inmunología , Análisis de Supervivencia , Regulación hacia Arriba
3.
Curr Opin Microbiol ; 64: 19-26, 2021 12.
Artículo en Inglés | MEDLINE | ID: mdl-34537517

RESUMEN

Citrobacter rodentium, a natural mouse pathogen which colonises the colon of immuno-competent mice, provides a robust model for interrogating host-pathogen-microbiota interactions in vivo. This model has been key to providing new insights into local host responses to enteric infection, including changes in intestinal epithelial cell immunometabolism and mucosal immunity. C. rodentium injects 31 bacterial effectors into epithelial cells via a type III secretion system (T3SS). Recently, these effectors were shown to be able to form multiple intracellular subnetworks which can withstand significant contractions whilst maintaining virulence. Here we highlight recent advances in understanding gut mucosal responses to infection and effector biology, as well as potential uses for artificial intelligence (AI) in understanding infectious disease and speculate on the role of T3SS effector networks in host adaption.


Asunto(s)
Infecciones por Enterobacteriaceae , Sistemas de Secreción Tipo III , Animales , Inteligencia Artificial , Citrobacter rodentium , Inmunidad , Ratones , Sistemas de Secreción Tipo III/genética , Virulencia
4.
Acta Trop ; 146: 73-80, 2015 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-25784560

RESUMEN

Toll-like receptor 2 (TLR2), a member of the Toll-like receptor family, plays an important role in the recognition of and subsequent immune response activation against leptospirosis in humans. The genetic polymorphism in TLR2 of an arginine to glutamine substitution at residue 753 (Arg753Gln) has been associated with a negative influence on TLR2 function, which may, in turn, determine the innate host response to Leptospira spp. This bacterium signals through TLR2/TLR1 heterodimers in human cells. The aim of the present study was to investigate the Arg753Gln single-nucleotide polymorphism (SNP) of the TLR2 gene, and the isoleucine to serine transversion at position 602 (Ile602Ser) of the TLR1 gene (previously associated with Lyme disease), in leptospirosis patients compared to healthy controls, carrying out a retrospective case/control study. The TLR2 polymorphism adenine (A) allele was observed in 7.3% of leptospirosis patients but was not found in the control group, whereas the guanine (G) allele of the TLR1 polymorphism was found in 63.6% of patients and 41.6% of controls. Susceptibility to leptospirosis disease was increased 10.57-fold for carriers of the TLR2 G/A genotype (P=0.0493) and 3.85-fold for carriers of the TLR1 G/G genotype (P=0.0428). Furthermore, the risk of developing hepatic insufficiency and jaundice was increased 18.86- and 27.60-fold for TLR2 G/A carriers, respectively. Similarly, the risk of developing jaundice was increased 12.67-fold for TLR1 G allele carriers (G/G and T/G genotypes). In conclusion, the present data suggest that the TLR2 Arg753Gln and TLR1 Ile602Ser SNPs influence the risk of developing leptospirosis and its severity.


Asunto(s)
Predisposición Genética a la Enfermedad , Inmunidad Innata/genética , Leptospirosis/genética , Leptospirosis/inmunología , Polimorfismo de Nucleótido Simple , Receptor Toll-Like 1/genética , Receptor Toll-Like 2/genética , Adolescente , Adulto , Anciano , Argentina , Estudios de Casos y Controles , Niño , Preescolar , Femenino , Humanos , Lactante , Masculino , Persona de Mediana Edad , Estudios Retrospectivos , Receptor Toll-Like 1/inmunología , Receptor Toll-Like 2/inmunología , Adulto Joven
5.
PLoS One ; 9(7): e102860, 2014.
Artículo en Inglés | MEDLINE | ID: mdl-25032961

RESUMEN

Leptospirosis is a global zoonosis caused by pathogenic Leptospira, which can colonize the proximal renal tubules and persist for long periods in the kidneys of infected hosts. Here, we characterized the infection of C57BL/6J wild-type and Daf1-/- mice, which have an enhanced host response, with a virulent Leptospira interrogans strain at 14 days post-infection, its persistence in the kidney, and its link to kidney fibrosis at 90 days post-infection. We found that Leptospira interrogans can induce acute moderate nephritis in wild-type mice and is able to persist in some animals, inducing fibrosis in the absence of mortality. In contrast, Daf1-/- mice showed acute mortality, with a higher bacterial burden. At the chronic stage, Daf1-/- mice showed greater inflammation and fibrosis than at 14 days post-infection and higher levels at all times than the wild-type counterpart. Compared with uninfected mice, infected wild-type mice showed higher levels of IL-4, IL-10 and IL-13, with similar levels of α-smooth muscle actin, galectin-3, TGF-ß1, IL-17, IFN-γ, and lower IL-12 levels at 90 days post-infection. In contrast, fibrosis in Daf1-/- mice was accompanied by high expression of α-smooth muscle actin, galectin-3, IL-10, IL-13, and IFN-γ, similar levels of TGF-ß1, IL-12, and IL-17 and lower IL-4 levels. This study demonstrates the link between Leptospira-induced murine chronic nephritis with renal fibrosis and shows a protective role of Daf1.


Asunto(s)
Antígenos CD55/metabolismo , Fibrosis/metabolismo , Enfermedades Renales/metabolismo , Túbulos Renales Proximales/metabolismo , Leptospirosis/metabolismo , Nefritis/metabolismo , Actinas/metabolismo , Animales , Fibrosis/microbiología , Galectina 3/metabolismo , Inflamación/metabolismo , Inflamación/microbiología , Interferón gamma/metabolismo , Interleucinas/metabolismo , Enfermedades Renales/microbiología , Túbulos Renales Proximales/microbiología , Leptospira interrogans , Leptospirosis/microbiología , Ratones , Ratones Endogámicos C57BL , Nefritis/mortalidad , Factor de Crecimiento Transformador beta1/metabolismo
7.
Neurosci Lett ; 501(3): 163-6, 2011 Sep 01.
Artículo en Inglés | MEDLINE | ID: mdl-21782004

RESUMEN

Argentine haemorrhagic fever (AHF) is a systemic febrile syndrome characterized by several haematological and neurological alterations caused by Junín virus (JUNV), a member of the Arenaviridae family. Newborn mice are highly susceptible to JUNV and the course of infection has been associated with the viral strain used. Galectin-3 (Gal-3) is an animal lectin that has been proposed to play an important role in some central nervous system (CNS) diseases. In this study, we analysed Gal-3 expression at the transcriptional and translational expression levels during JUNV-induced CNS disease. We found that Candid 1 strain induced, with relatively low mortality, a subacute/chronic CNS disease with significant glia activation and upregulation of Gal-3 in microglia cells as well as in reactive astrocytes that correlated with viral levels. Our results suggest an important role for Gal-3 in viral-induced CNS disease.


Asunto(s)
Infecciones por Arenaviridae/metabolismo , Encefalitis Viral/metabolismo , Galectina 3/biosíntesis , Virus Junin/patogenicidad , Neuroglía/metabolismo , Neuroglía/virología , Regulación hacia Arriba/fisiología , Animales , Animales Recién Nacidos , Infecciones por Arenaviridae/patología , Astrocitos/metabolismo , Astrocitos/patología , Astrocitos/virología , Modelos Animales de Enfermedad , Encefalitis Viral/patología , Fiebre Hemorrágica Americana/metabolismo , Fiebre Hemorrágica Americana/patología , Fiebre Hemorrágica Americana/virología , Ratones , Ratones Endogámicos C57BL , Microglía/metabolismo , Microglía/patología , Microglía/virología , Neuroglía/patología
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