Resilience of three indigenous goat breeds to heat stress based on phenotypic traits and PBMC HSP70 expression.

The objective of this experiment was to evaluate the influence of summer heat stress on physiological and behavioral responses of Osmanabadi, Salem Black, and Malabari goats. The study also evaluated the differences in heat shock protein 70 (HSP70) expression pattern between these breeds. The study was conducted over 45 days during summer (April-May) using 36 1-year-old female goats by randomly allocating them into six groups with six animals in each group: Osmanabadi control (Osmanabadi CON), Osmanabadi heat stress (Osmanabadi HS), Malabari control (Malabari CON), Malabari heat stress (Malabari HS), Salem Black control (Salem Black CON), and Salem Black heat stress (Salem Black HS). The Osmanabadi CON, Malabari CON, and Salem Black CON animals were housed in a shed while the Osmanabadi HS, Malabari HS, and Salem Black HS groups were subjected to heat stress by exposing them to outside environment between 1000 and 1600 h during the experimental period. All 36 animals were provided with ad libitum feed and water. The data generated were analyzed by general linear model (GLM) repeated measurement analysis of variance. Results indicated that the drinking frequency (DF) was higher (p < 0.01) in heat stress groups (12.58, 12.25, and 10.75 times for the Osmanabadi HS, Malabari HS, and Salem Black HS, respectively) as compared to their respective control groups (5.67, 6.25, 5.58 times for the Osmanabadi CON, Malabari CON, and Salem Black CON, respectively). Water intake (WI) also showed similar trend to DF. The urinating frequency also (UF) differed between breeds with lower value (p < 0.05) recorded in the Salem Black HS (1.5 times) compared to the Malabari HS (2.92 times). The highest (p < 0.05) rumination time (RuT) was recorded in the Malabari HS (48.00 min) than both the Osmanabadi HS (20.91 min) and Salem Black HS (23.67 min). The heat stress increased (p < 0.05) all physiological variables at 1400 h. The findings of this study suggest RR, RT, and PBMC HSP70 are reliable biological markers for evaluating thermo-tolerance capacity of indigenous goat breeds.

Comparative assessment of heat stress induced changes in carcass traits, plasma leptin profile and skeletal muscle myostatin and HSP70 gene expression patterns between indigenous Osmanabadi and Salem Black goat breeds.

The primary objective of the study was to compare the impact of heat stress on meat production characteristics of Osmanabadi and Salem Black breed goats based on changes in carcass characteristics, meat quality attributes, plasma leptin concentration, skeletal muscle myostatin and heat shock protein 70 (HSP70) gene expression patterns. The goats were randomly distributed into four groups: OSC (n = 6; Osmanabadi Control), OSHS (n = 6; Osmanabadi Heat Stress), SBC (n = 6; Salem Black Control) and SBHS (n = 6; Salem Black Heat Stress). The animals were slaughtered at the end of the study and their meat characteristics were assessed. This study established the impact of heat stress on a wide variety of carcass and meat quality characteristics in OS and SB goat breeds. The results from the study also provided some crucial evidence for a better resilience capacity of Salem Black breed as compared to Osmanabadi goats in maintaining the meat production during heat stress. The study also established plasma leptin and HSP70 genes to be the ideal biomarkers to reflect the impact of heat stress on meat characteristics in indigenous goats.