Understanding the molecular mechanisms for the enhanced phytoremediation of heavy metals through plant growth promoting rhizobacteria: A review.

Rapid industrialization, modern agricultural practices and other anthropogenic activities add a significant quantity of toxic heavy metals into the environment, which induces severe toxic effects on all form of living organisms, alter the soil properties and its biological activity. Remediation of heavy metal contaminated sites has become an urgent necessity. Among the existing strategies, phytoremediation is an eco-friendly and much convincing tool for the remediation of heavy metals. However, the applicability of phytoremediation in contaminated sites is restricted by two prime factors such as i) slow growth rate at higher metal contaminated sites and ii) metal bioavailability. This circumstance could be minimized and accelerate the phytoremediation efficiency by incorporating the potential plant growth promoting rhizobacterial (PGPR) as a combined approach. PGPR inoculation might improve the plant growth through the production of plant growth promoting substances and improve the heavy metal remediation efficiency by the secretion of chelating agents, acidification and redox changes. Moreover, rhizobacterial inoculation consolidates the metal tolerance and uptake by regulating the expression of various metal transporters, tolerant and metal chelator genes. However, the exact underlying molecular mechanism of PGPR mediated plant growth promotion and phytoremediation of heavy metals is poorly understood. Thus, the present review provides clear information about the molecular mechanisms excreted by PGPR strains in plant growth promotion and phytoremediation of heavy metals.

Evaluation of Cr(VI) reduction mechanism and removal by Cellulosimicrobium funkei strain AR8, a novel haloalkaliphilic bacterium.

The present study, a novel haloalkaliphilic Cr(VI) tolerant bacterial strain, Cellulosimicrobium funkei AR8, was isolated and characterized for its high Cr(VI) reduction. In batch experiments, Cr(VI) reduction was evaluated under different parametric conditions which include different pH (5-9), temperature (25-45°C), NaCl (0-3%) and Cr(VI) concentrations (100-250µg/ml). Variations in the cell surface functional groups and morphology of the bacterial cells after Cr(VI) reduction were characterized by FT-IR and SEM-EDX. FT-IR analysis revealed that cell surface functional groups such as alkanes, amide and amines are involved in chromium biosorption and SEM-EDX results showed that biosorption and immobilization of chromium species on the cell surface. Bioconversion of Cr(VI) into Cr(III) by strain AR8 was confirmed by XRD and Raman spectroscopy analysis. Intracellular localization of reduced product (Cr(III)) was visualized by TEM analysis. Various instrumentation analysis verified that Cr(VI) removal mechanism of C. funkei AR8 strain was achieved by both extra and intracellular reducing machinery. Toxicity study revealed that the bacterially reduced product exerted less toxic effects on phenotypic, survival (91.31%), hatching (84.04%) and heart function (115±1.03 beats/min) of zebrafish (Danio rerio) embryos. Higher Cr(VI) reducing ability of the strain under haloalkaliphilic condition suggests the C. funkei AR8 as a novel and efficient strain for remediating Cr(VI) contaminated industrial effluents with high salinity and alkalinity.

Characterization of multifarious plant growth promoting traits of rhizobacterial strain AR6 under Chromium (VI) stress.

Plant growth promoting rhizobacteria (PGPR) can increase the host plant tolerance to cope up with heavy metal induced stress, which can be improve plant growth. Thus, the present study was designed to isolate Cr(VI) tolerant PGPR strain and evaluate its plant growth promoting (PGP) properties under Cr(VI) stress. Rhizobacterial strain AR6 was isolated from the rhizosphere of Phaseolus vulgaris L. and showed 99% homology with Cellulosimicrobium funkei (KM032184) in BLASTn analysis. Strain AR6 was specifically selected due to its high Cr(VI) tolerance (1200µg/ml) and substantial production of PGP substances. Strain AR6 produced 36.75µg/ml of indole acetic acid (IAA), 60.40µg/ml of ammonia and 14.23µg/ml of exopolysaccharide (EPS). Moreover, strain AR6 showed positive results for catalase, protease, amylase, lipase production and phosphate solubilization. A trend of Cr(VI) concentration dependent progressive decline for PGP traits of strain AR6 was observed excluding EPS which was regularly increased on increasing concentrations of Cr(VI). Among the four tested Cr(VI) concentrations, 250µg/ml showed the maximum toxicity to PGP activities of strain AR6. Inoculation of rhizobacterial strain AR6 significantly increased the root length of test crops in the presence of Cr(VI) and produced a considerable number of colonizes on the root of versatile dicot and monocot plants. Moreover, strain AR6 exhibited strong antagonistic activity against phytopathogen Aspergillus niger. Thus, the present study suggests that metal tolerant and PGP activities of the rhizobacterial strain AR6 could be exploited for environmental and agricultural issues.